• Archives of Pharmacal Research
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• 제21권6호
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• pp.707-711
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• 1998

The (Na,K)ATPase is responsible for generating the ionic gradients and membrane potentials by the exchange of intracellular $Na^+$ for $K^+$. It has been recentl y shown that (Na,K)ATPase is involved in the exocytic pathway of basic fibroblast growth factor (bFGF), although it is not known that bFGF is secreted to the outside of cell through direct interaction with (Na,K) ATPase. To understand the role for (Na,K)ATPase in the secretary pathway of bFGF, we have sought to identify the cytoplasmic domains of the alpha1 isoform of (Na,K)ATPase interacting with bFGF by yeast two-hybrid system. We have also investigated the interaction between the alpha2 isoform of (Na,K)ATPase and bFGF to find out whether the interaction is isoform-specific. We found that none of the cytoplasmic domains of (Na,K)ATPase isoforms interacted with bFGF. The result suggests that the interaction between bFGF and (Na,K)ATPase might be indirect, thus requiring other proteins which are involved in the formation of protein complexes for the interaction, although we cannot exclude the possibility that the interaction requires the element of the whole alpha subunit structure that was not present in the isolated alpha subunit cytoplasmic domains.

• Journal of applied mathematics & informatics
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• 제6권1호
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• pp.31-50
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• 1999

We revisit the problems of testing three-factor classifica-tion models with a single observation per cell. A common approach in analyzing such nonreplicated data is to omit the highest order in-teraction and regard it as error. This paper discusses the use of a multiplicative model(See and Smith 1996 and 1998) which is applied on residuals in order to separate the variablility due to three-factor interaction from what is counted as random error. in particualr to test the significance of the interaction term we derived an approxi-mated distribution of the likelihood ratio test statistic based on the quadrilinear model known as Tucher's three-mode principal compo-nent model. The derivation utilizes the distribution of the eignevalues of the Wishart matrix.

• 복식문화연구
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• 제5권2호
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• pp.195-205
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• 1997

The purpose of this study was to identify the effect of genderand body cathexis on clothing behavior (interest in clothing and clothing satisfaction/dissatisfaction). The questionnaire were administered to 395 college students living in Jechon. Data were analyzed by frequency, factor analysis, t-test, ANOVA, two-way ANOVA, Duncan's Multiple Range Test and Pearson's Correlation. The results of this study were as follows : 1) body-cathexis was higher in men than women. In general, students were more satisfied with upper body parts than lower body parts. 2) There was no relationship between interest in cloghing and body-cathexis. But woman showed more interest in clothing than man, therefore sex showed main effect. And there was interaction between sex and body-cathexis on interest in clothing. 3) Clothing satisfaction was devided into aesthetic/economical/practical factors. Among three satisfaction factors, body cathexis has the main effect in economic factor, and there was no interaction. But in aesthetic factor, gender and body-cathexis showed interaction and body-cathexis showed main effect.

• 대한간호학회지
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• 제16권3호
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• pp.31-37
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• 1986

The purpose of this study is to develop a ratio scale measuring organizational charateristics in nursing. 599 staff nurses working at 10 educational hospitals in Seoul were participated during the period from July 1 to July 31, 1985. Subjects were instructed to rate at one to five points likert type scale according to the level of positive characteristics affecting the nursing organizational effectiveness. As a result of factor analysis given to 50 items with varimax rotation and rearrangement on the asis of factor loading 0.3, there are 4 factors consisted of 33 items presented: factor 1 : leadership of head nurses and interaction(11 items) factor 2 : leadership of supervisors and top managers(11 items) factor 3 : interaction with supervisors(5 items) factor 4 : organizational climate(6 items) Reliability of the factors were tested by Cronbach's Alpha, and the result was α=0.67∼0.84 Further studies are needed to identify the relationship between this tool and the effectiveness of various organization in nursing.

• 대한가정학회지
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• 제43권4호
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• pp.203-223
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• 2005

This study investigated the leisure constraints and leisure life satisfaction of the institutional elderly. The respondents were 302 elderly people residing in 5 free institutionalized elderly homes in Seoul. The data were analyzed by frequency analysis, factor analysis, One-way ANOVA, Duncan's post hoc test and regression analysis using SPSS WIN 12.0 program. Exploratory factor analysis revealed that the 10 items in the proposed scale could be grouped into four factors of constraint on leisure:'physical constraint','psychological constraint','interpersonal constraint', and 'structural constraint 'There were differences in leisure constraints according to sex, age, education degree, interaction with facility colleagues, and interaction with facility staff. There were differences in leisure life satisfaction according to sex, age, having religion or not, interaction with facility colleagues, interaction with facility staff and leisure constraints. Regression analysis results indicated that the main effective factors of leisure life satisfaction were leisure constraints.

• 대한기계학회논문집A
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• 제24권5호
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• pp.1288-1296
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• 2000

In this study, fatigue crack behavior between arbitrarily located defects was investigated by experiment. Especially, stress interaction between micro hole defects and fatigue cracking, and fatigue crack initiation life following the variation of location of micro hole defects were considered. In addition, crack initiation position by micro hole stress interaction and the relationship between stress concentration factor and fatigue initiation life are studied in detail.

• 대한가정학회지
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• 제50권7호
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• pp.109-116
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• 2012

The purpose of this study is to develop a scale for evaluation of Parent's Literacy Interaction Rating Scale (PLIRS) for preschool children and to examine its validity and reliability. The participants of this study were 342 Korean mothers with children of 3 to 5 year olds. The methods for data analysis included item analysis, factor analysis for construct validity, Pearson correlations between PLIRS and two sub tests of EC-HOME for concurrent validity, and Cronbach's ${\alpha}$ for reliability. The item discrimination was determined by comparing the highest and lowest groups using Chi-square (${\chi}^2$), and Cramer's V. The 25 items of the scale were found to be satisfactory in item discrimination. The concurrent validity was also identified by correlation between PLIRS and two sub tests of EC-HOME. Factor analysis revealed that the structure of the PLIRS consisted of three factors: 'open interaction', 'directive interaction', and 'picture-book reading interaction'. 'Open interaction' is based on 'Whole Language Approach', such as 'I have my child write as he/she likes even if they spell wrongly.' 'Directive interaction' is based on 'Code Emphasis Approach', such as 'I have my child take dictation.' 'Picture-book reading interaction' is how parents read picture-book with her/his children, such as 'When I read a picture book to my child, I ask various questions about a book story.' The Cronbach's ${\alpha}$ for internal consistency reliability range was .78 to .87 for three subscales. It was concluded that PLIRS was valid and reliable to examine the literacy interaction between parents and children in home.

• BMB Reports
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• 제48권6호
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• pp.342-347
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• 2015

The expression of epidermal growth factor receptor (EGFR) is an important diagnostic marker for triple-negative breast cancer (TNBC) cells, which lack three hormonal receptors: estrogen and progesterone receptors as well as epidermal growth factor receptor 2. EGFR transactivation can cause drug resistance in many cancers including TNBC, but the mechanism underlying this phenomenon is poorly defined. Here, we demonstrate that insulin treatment induces EGFR activation by stimulating the interaction of EGFR with insulin-like growth factor receptor 1 (IGF-1R) in the MDA-MB-436 TNBC cell line. These cells express low levels of EGFR, while exhibiting high levels of IGF-1R expression and phosphorylation. Low-EGFRexpressing MDA-MB-436 cells show high sensitivity to insulinstimulated cell growth. Therefore, unexpectedly, insulin stimulation induced EGFR transactivation by regulating its interaction with IGF-1R in low-EGFR-expressing TNBC cells. [BMB Reports 2015; 48(6): 342-347]

• 대한교통학회지
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• 제6권2호
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• pp.35-48
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• 1988

Amongst various analytic methods of internal structure of city, the factor analysis method which uses O-D matrix data has some merits and characteristics compared to other methods. 1) It is possible to find one certain interaction and flow pattern between traffic zones with in a city through reanalyzing O-D data which is too complex to grasp specific meaning or pattern of flow systems. 2) It can be easily visualized the traffic flow pattern by using adequate graphic techniques, and also can clarify the functional areas whose interaction linkages are significantly strong enough between each other. In this study, the taxi traffic O-D data between 42 traffic zones in Kwang-ju city was reanalyzied by varimax rotated factor analysis methods. As a result, four factors that have significant level factor loading (over 0.5 ) and factor score (over 1.0) were sorted out. so to speak four different functional areas were clarified in Kwang-ju city, of the West, the East, the south, and the North functional areas whose interaction linkages are significantly strong enough between each other. In the study, the taxi traffic O-D data between 42 traffic zones in Kwang-ju city was reanalyzied by varimax rotated factor analysis methods. As a result, four factors that have significant level factor loading (over 0.5) and factor score 9over 1.0) were sorted out. so to speak four different functional areas were clarified in Kwang-ju city, of the West, the East, the South, and the North functional area, then these four functional areas are almost coincided with citizen's general conception of community division and administrative district. Accordingly the factor analysis methods using traffic data seems to proved to be very accurate and useful analytic instruments for analyzing flow pattern and clarifying functional areas of city, and believed to provide basic informations and criteria for practical urban land use planning and transportation planning.

• 한국미생물·생명공학회지
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• 제33권4호
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• pp.255-259
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• 2005

본 실험에서는 HRF의 조절단백질을 알아보기 위해 HRF를 bait로 한 yeast two hybrid assay를 실행한 결과 해당과정에 관여하는 TPI(triosephosphate isomerase)라는 효소를 발견하였으며, 가장 많이 중복되어 있었다. In vitro에서 HRF는 TPI의 C말단 잔기 부근(아미노산 156-249)이 상호작용에 주로 관여하는 부위임을 알 수 있었다. 또한, HeLa 세포에서 immunoprecipitation을 이용하여 HRF와 TPI의 상호작용이 실제 in vivo에서도 일어나는 현상이라는 것을 밝혔다. 결과적으로 HRF와 TPI 상호작용은 세포내 일정량이 존재하며 여러가지 신호전달에 의해 동시에 Na,K-ATPase와도 상호작용하는 것으로 생각된다.