• Bulletin of the Korean Chemical Society
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• 제32권4호
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• pp.1158-1164
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• 2011

5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT), a psychoactive tryptamine derivative, is a hallucinogenic drug of abuse. In this study, 5-OH-DIPT and its metabolites were identified and the quantitative method was developed and validated by using hydrophilic interaction chromatography-tandem mass spectrometry (HILICMS/MS). Chromatographic separation was achieved on an Atlantis HILIC silica column ($5{\mu}m$, $100{\times}2.1\;mm$). The metabolites of 5-MeO-DIPT in rat urine were characterized via Q1 scanning and product ion scanning. As a consequence, 5-MeO-IPT, 5-OH-DIPT, 6-OH-5-MeO-DIPT and their glucuronide conjugates were detected and identified as the metabolites of 5-MeO-DIPT. Subsequently, a quantitative method for 5-MeO-DIPT and its major metabolites, 5-MeO-IPT and 5-OH-DIPT, was developed in multiple reactions monitoring (MRM) mode. The calibration curves for all analytes evidenced good linearity over the concentration range of 1-1000 ng/mL with linear correlation co-efficients ($r^2$) in excess of 0.99. The intra- and inter-day accuracy and precision were 92.2-110.2% and 1.5-9.9%, respectively.

• Earthquakes and Structures
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• 제14권5호
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• pp.411-424
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• 2018

This study explores the inelastic behavior of systems with flexible base. The use of a single degree of freedom system (ESDOF) with equivalent ductility to represent the response of flexible base systems is discussed. Two different equations to compute equivalent ductility are proposed, one which includes the contribution of rigid body components, and other based on the overstrength of the structure. In order to asses the accuracy of ESDOF approach with the proposed equations, the behavior of a 10-story regular building with reinforced concrete (RC) moment resisting frames is studied. Local and global ductility capacity and demands are used to study the modifications introduced by base flexibility. Three soil types are considered with shear wave velocities of 70, 100 and 250 m/s. Soil-foundation stiffness is included with a set of springs on the base (impedance functions). Capacity curves of the building are computed with pushover analysis. In addition, non linear time history analysis are used to asses the ductility demands. Results show that ductility capacity of the soil-structure system including rigid body components is reduced. Base flexibility does not modify neither yield and maximum base shear. Equivalent ductility estimated with the proposed equations is fits better the results of the numerical model than the one considering elastoplastic behavior. Modification of beams ductility demand due to base flexibility are not constant within the structure. Some elements experience reduced ductility demands while other elements experience increments when flexible base is considered. Soil structure interaction produces changes in the relation between yield strength reduction factor and structure ductility demand. These changes are dependent on the spectral shape and the period of the system with fixed and flexible base.

• International Journal of Fluid Machinery and Systems
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• 제10권3호
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• pp.287-295
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• 2017

The most detrimental pressure pulsations in high-head pump-turbines is caused by the rotor-stator interaction (RSI) between the guide vanes and runner blades. When the pump-turbine operates in the S-shaped region of the characteristic curves, the deteriorative flow structures may significantly strengthen RSI, causing larger pressure pulsations and stronger vibration with an increased risk of mechanical failure. CFD simulations were carried out to analyze the impacts of flow evolution on the pressure pulsations in the S-shaped region of a model pump-turbine. The results show that the reverse flow vortex structures (RFVS) at the runner inlet have regular development and transition patterns when discharge reduces from the best efficiency point (BEP). The RFVS first occur at the hub side, and then shift to the mid-span near the no-load point, which cause the strongest pressure pulsations. The locally distributed RFVS at hub side enhance the local RSI and makes the pressure fluctuations at the corresponding sections stronger than those at the rest sections along the spanwise direction. Under the condition of RFVS at the mid-span, the smaller flow rate make the smaller difference of pressure pulsation amplitudes in the spanwise direction. Moreover, the rotating stall, rotating at 35.7%-62.5% of the runner rotational frequency, make the low frequency components of pressure pulsations distribute unevenly along the circumference in the vaneless space. However, it have little influence on the distributions of high components.

• 한국해안·해양공학회논문집
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• 제25권4호
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• pp.236-243
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• 2013

해상풍력발전기는 주위를 항행하는 선박 및 바지선등과 같은 선박에 의한 충돌피해가 발생할 수 있기 때문에 이에 대한 안정성을 고려해야 한다. 본 연구에서는 선박충돌에 대해 안정성을 고려하기 위해 해상풍력발전기의 선박충돌해석을 수행하고 충돌하중의 불확실성을 고려하기 위해 충돌취약도를 분석하였다. 충돌해석은 해저지반-기초구조물의 상호작용 및 유체를 p-y곡선과 부가질량법으로 고려하였다. 충돌취약도는 선박의 중량과 충돌각, 선박흘수를 변동성으로 고려하여 항복응력에 대한 손상수준을 추정하였으며 취약도를 분석한 결과, 850ton 바지선과 30,000DWT 화물선의 충돌속도에 취약함을 확인하였다.

• 한국지반환경공학회 논문집
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• 제13권6호
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• pp.73-81
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• 2012

해상풍력시스템의 규모가 점차적으로 증가하고 있는 추세에 따라 상부구조물을 지지하는 기초구조물 안정성 확보의 중요성이 대두되고 있다. 이에 따라, 지반공학적 측면에서 기초구조물과 지반의 상호작용이 전체 시스템에 미치는 영향을 파악하기 위한 해석방안이 요구되고 있으며, p-y 곡선 모델의 적용이 대표적인 방법이다. 본 연구에서는 풍황이 우수하여 해상풍력발전 시스템 운영을 위한 대상지로써 적합하다고 알려져 있는 제주도 해저지반(현무암)의 공학적 특성을 현재까지 제안된 암반 p-y 곡선 모델에 적용하였다. 이를 통해 제주도 및 국내 암반 분포 지역에 대한 해상풍력시스템 기초구조물과 해저암반 간의 상호작용 해석 과정을 정리하고 향후 연구 방향을 제안하였다. 전반적으로 암반 p-y 곡선 산정결과는 지표면에 근접할수록 지반반력이 증가하는 경향을 나타냈으며, 말뚝에 작용하는 모멘트, 전단력 등을 분석한 결과 암반의 공학적 특성을 적절히 모사할 수 있음을 확인하였다.

• 약학회지
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• 제43권5호
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• pp.642-651
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• 1999

Nonsedating antihistamines do net cause sedation in therapeutic doses because these drugs hardly cross the blood-brain barrier. Since most of the peripheral side dffects of conventional antihistamines are related to their muscarinic receptor blocking action, the present study was performed to investigate whether nonsedating antihistamines interact with the muscarinic receptors and discriminate the muscarinic receptor subtypes in the rat cerebral microsomal fraction which containes both $M_1,{\;}M_2,{\;}M_3{\;}and{\;}M_4$ receptors. Five nonsedating antihistamines at high concentrations inhibited [$^3H$]QNB binding to the muscarinic receptor in a dose-dependent manner. The inhibition curves of these drugs except loratadine which showed positive cooperativity (nH=1.55) were steep (nH=1), indicating interaction with a single homogenous population of the binding sites. Astemizole, clemizole and mequitazine increased the $K_D$ value for [$^3H$]QNB without affecting the binding site concentrations, and this increase in the $K_D$ value resulted from the ability of these drugs to slow [$^3H$]QNB-receptor association. The Ki values of astemizole, clemizole and mequitazine for the inhibition for the inhibition of [$^3H$]QNB binding to muscarinic receptor were 0.58, 5.99 and $0.007{\;}{\mu}M$, respectively. However, loratadine and terfenadine inhibited noncompetitively [$^3H$]QNB binding with the normalized $IC_50$ value of about $2{\;}{\mu}M$. These results demonstrate that; 1) astemizole, clemizole and mequitazine interact directly with the muscarinic receptor at high concentrations; 2) muscarinic receptor blocking potency of these drugs varies widely among drugs; 3) these drugs do not discriminate between muscarinic receptor subtypes.

• 한국지반공학회논문집
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• 제34권5호
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• pp.53-63
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• 2018

무리말뚝의 내진설계를 수행할 때 지반-말뚝 동적상호작용을 고려하는 것이 중요하다. 특히, 동적하중을 받는 무리말뚝의 횡방향 저항력은 무리말뚝 효과에 의하여 단일말뚝과 비교하여 감소한다. 그러나, 지금까지 지진하중을 받는 무리말뚝의 동적 무리말뚝 효과를 제안한 연구는 매우 부족한 실정이다. 그러므로, 본 연구에서는 건조 모래지반에 설치된 $3{\times}3$ 무리말뚝에 대한 동적 원심모형실험을 수행하여 무리말뚝 효과를 산정하였다. 이 무리말뚝 효과는 동적 p-y 곡선에서 극한 횡방향 지반반력과 지반반력계수에 대한 보정계수(multiplier)를 적용하여 고려하였다. 그리고, 본 연구에서 얻어진 동적 p-y 곡선을 Beam on Nonlinear Winkler Foundation 모델을 이용한 비선형 동해석에 적용하여 그 적용성을 검증하였다. 그 결과, 본 연구에서 제안한 무리말뚝의 보정계수가 원심모형실험 결과를 잘 모사할 수 있는 것으로 나타났다.

• 한국재료학회지
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• 제22권7호
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• pp.329-334
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• 2012

Two types of nanoclusters, termed Cluster (1) and Cluster (2) here, both play an important role in the age-hardening behavior in Al-Mg-Si alloys. Small amounts of additions of Cu and Ag affect the formation of nanoclusters. Two exothermic peaks were clearly detected in differential scanning calorimetry(DSC) curves by means of peak separation by the Gaussian method in the base, Cu-added, Ag-added and Cu-Ag-added Al-Mg-Si alloys. The formation of nanoclusters in the initial stage of natural aging was suppressed in the Ag-added and Cu-Ag-added alloys, while the formation of nanoclusters was enhanced at an aging time longer than 259.2 ks(3 days) of natural aging with the addition Cu and Ag. The formation of nanoclusters while aging at $100^{\circ}C$ was accelerated in the Cu-added, Ag-added and Cu-Ag-added alloys due to the attractive interaction between the Cu and Ag atoms and the Mg atoms. The influence of additions of Cu and Ag on the clustering behavior during low-temperature aging was well characterized based on the interaction energies among solute atoms and on vacancies derived from the first-principle calculation of the full-potential Korrinaga-Kohn-Rostoker(FPKKR)-Green function method. The effects of low Cu and Ag additions on the formation of nanoclusters were also discussed based on the age-hardening phenomena.

• 분석과학
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• 제34권5호
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• pp.193-201
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• 2021

We have demonstrated a sensitive analytical method of measuring oleracone D in mouse plasma using a liquid chromatography-tandem mass spectrometry (LC-MS/MS). Oleracone D and oleracone F (internal standard) in mouse plasma samples were processed using a liquid-liquid extraction method with methyl tertbutyl ether, resulting in high and reproducible extraction recovery (80.19-82.49 %). No interfering peaks around the peak elution time of oleracone D and oleracone F were observed. The standard calibration curves for oleracone D ranged from 0.5 to 100 ng/mL and were linear with r² of 0.992. The inter- and intra-day accuracy and precision and the stability fell within the acceptance criteria. The pharmacokinetics of oleracone D following intravenous and oral administration of oleracone D at doses of 5 mg/kg and 30 mg/kg, respectively, were investigated. When oleracone D was intravenously injected, it had first-order elimination kinetics with high clearance and volume of distribution values. The absolute oral bioavailability of this compound was calculated as 0.95 %, with multi-exponential kinetics. The low aqueous solubility and a high oral dose of oleracone D may explain the different elimination kinetics of oleracone D between intravenous and oral administration. Collectively, this newly developed sensitive LC-MS/MS method of oleracone D could be successfully utilized for investigating the pharmacokinetic properties of this compound and could be used in future studies for the lead optimization and biopharmaceutic investigation of oleracone D.

• Wind and Structures
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• 제27권6호
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• pp.381-397
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• 2018

Nonlinear behavior in fluid-structure interaction (FSI) of bridge decks becomes increasingly significant for modern bridges with increasing spans, larger flexibility and new aerodynamic deck configurations. Better understanding of the nonlinear aeroelasticity of bridge decks and further development of reduced-order nonlinear models for the aeroelastic forces become necessary. In this paper, the amplitude-dependent and neutral angle dependent nonlinearities of the motion-induced loads are further highlighted by series of computational fluid dynamics (CFD) simulations. An effort has been made to investigate a semi-analytical time-domain model of the nonlinear motion induced loads on the deck, which enables nonlinear time domain simulations of the aeroelastic responses of the bridge deck. First, the computational schemes used here are validated through theoretically well-known cases. Then, static aerodynamic coefficients of the Great Belt East Bridge (GBEB) cross section are evaluated at various angles of attack, leading to the so-called nonlinear backbone curves. Flutter derivatives of the bridge are identified by CFD simulations using forced harmonic motion of the cross-section with various frequencies. By varying the amplitude of the forced motion, it is observed that the identified flutter derivatives are amplitude-dependent, especially for $A^*_2$ and $H^*_2$ parameters. Another nonlinear feature is observed from the change of hysteresis loop (between angle of attack and lift/moment) when the neutral angles of the cross-section are changed. Based on the CFD results, a semi-analytical time-domain model for describing the nonlinear motion-induced loads is proposed and calibrated. This model is based on accounting for the delay effect with respect to the nonlinear backbone curve and is established in the state-space form. Reasonable agreement between the results from the semi-analytical model and CFD demonstrates the potential application of the proposed model for nonlinear aeroelastic analysis of bridge decks.