• Transactions of the Korean Society of Mechanical Engineers
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• v.16 no.5
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• pp.899-909
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• 1992

An iterative solution technique is presented to analyze the dynamic systems of rigid bodies subjected to kinematic constraints. Lagrange multipliers associated with the constraints are iteratively computed by monotonically reducing an appropriately defined constraint error vector, and the resulting equation of motion is solved by a well-established ODE technique. Constraints on the velocity and acceleration as well as the position are made to be satisfied at joints at each time step. Time integration is efficiently performed because decomposition or orthonormalization of the large matrix is not required at all. An acceleration technique is suggested for the faster convergence of the iterative scheme.

• Journal of Microbiology
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• v.40 no.3
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• pp.245-247
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• 2002

A genomic DNA library of the fungus Trametes versicolor was constructed in a yeast integration vector which contains the URA3 gene of the budding yeast Saccharomyces cerevisiae and the gene responsible for hygromycin B resistance, and fragments acting as autonomously replicating sequences (ARSes) in the budding yeast were identified from the genomic DNA library. Sixteen recombinant plasmids from the library transformed the budding yeast Saccharomyces cerevisiae to Ura+ at high frequencies. They were maintained stably under selective conditions, but were gradually lost from yeast cells at different rates under nonselective conditions, indicating that they contain eukaryotic origins of DNA replication and exist as extrachromosomal plasmids. Base sequences of four ARS DNAs among the 16 cloned fragments revealed that all or the four contain at least one 11 bp ［(A/T)TTTA(T/C)(A/G)TTT(A/T)］consensus sequence of the budding yeast ARS.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.2
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• pp.154-159
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• 2006

Interleukin-18 (IL-18), otherwise known as interferon-gamma-inducing factor (IGIF), is one of several well characterized and important cytokines that contribute to host defenses. The complementary DNA (cDNA) of mature human interleukin-18 gene (hIL-18) was fused with the signal peptide of the rice amylase 1A gene (Ramy1A) and introduced into the plant expression vector under the control of a duplicated CaMV 35S promoter. The recombinant plasmid was transformed into tobacco (Nicotiana tabacum L. cv Havana) using the Agrobacterium-mediated transformation method. The integration of the hlL-18 gene into the genome of transgenic tobacco plants was confirmed by polymerase chain reaction (PCR) amplification and its expression was observed in the suspension cells that were derived from the transgenic plant callus by using Northern blot analysis. The hlL-18 protein was detected in the extracts of the transgenic callus and in the medium of the transgenic tobacco suspension culture by using immunoblot analysis. Based upon enzyme-linked immunosorbant assay (ELISA) results, the expression level of the hlL-18 protein approximated $166{\mu}g/L$ in the suspension culture medium. Bioassay results from the induction of $interferon-{\gamma}$ from a KG-1 cell line indicated that the hlL-18 secreted into the suspension culture medium was bioactive.

• Proceedings of the Korea Information Processing Society Conference
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• 2001.04b
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• pp.611-614
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• 2001

최근 인터넷 사용의 폭발적인 증가로 인하여 웹 상에서 유기적으로 통합된 멀티미디어 컨텐츠에 대한 서비스 제공의 필요성이 대두되었다. 이에 1998년 W3C(World Wide Web Consortium)는 통합 멀티미디어 표현 언어인 SMIL(Synchronized Multimedia Integration Language)을 발표하였고, SMIL-Boston으로 진행했던 SMIL 1.0 확장 프로젝트를 SVG(Scalable Vector Graphics)의 애니메이션 기능의 도입 및 기능 확장으로 2000년 9월 SMIL 2.0으로 최종 Working Draft안을 발표하게 되었다. 이에 본 논문에서는 SMIL컨텐츠 저작을 위한 자동 생성 모듈 및 전용 저작 도구를 제공하고, SMIL 컨텐츠 데이터베이스 서버 및 재생기와의 통합으로 원활한 SMIL 컨텐츠 서비스 환경을 제공하는 웹 멀티미디어 컨텐츠 서비스를 위한 서버/클라이언트 통합 시스템을 설계 및 구현하였다.

• Journal of Plant Biotechnology
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• v.43 no.1
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• pp.66-75
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• 2016

Agrobacterium-mediated gene transfer has recently been developed to improve rice transformation. In this study, 3 different transformation methods were tested including soaking, co-cultivation, and vacuum infiltration. Agrobacterium tumefaciens GV3101 harboring the binary vector pGreen:: LeGSNOR was used in this experiment. This study aimed to identify the most appropriate method for transferring LeGSNOR into rice. Vacuum infiltration of the embryonic calli for 5 min in Ilpum resulted in high transformation efficiency based on confirmation by PCR, RT-PCR, and qRT-PCR analyses. In conclusion, we described the development of an efficient transformation protocol for the stable integration of foreign genes into rice; furthermore, the study results confirmed that PCR is suitable for efficient detection of the integrated gene. The vacuum infiltration system is a potentially useful tool for future studies focusing on transferring important genes into rice seed calli, and may help reduce time and effort.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.28 no.3
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• pp.385-392
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• 2010

A LiDAR technique has the merits that survey engineers can get a large number of measurements with high precision quickly. Aerial photos and satellite sensor images are used for generating 3D spatial images which are matched with the map coordinates and elevation data from digital topographic files. Also, those images are used for matching with 3D spatial image contents through perspective view condition composed along to the designated roads until arrival the corresponding location. Recently, 3D aviation image could be generated by various digital data. The advanced geographical methods for guidance of the destination road are experimented under the GIS environments. More information and access designated are guided by the multimedia contents on internet or from the public tour information desk using the simulation images. The height data based on LiDAR is transformed into DEM, and the real time unification of the vector via digital image mapping and raster via extract evaluation are transformed to trace the generated model of 3-dimensional downtown building along to the long distance for 3D tract model generation.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.5 no.2
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• pp.107-120
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• 1993

This paper presents a new modal solution of linear three-dimensional hydrodynamic equations using similarity transform technique. The governing equations are first separated into external and internal mode equations. The solution of the internal mode equation then proceeds as in previous modal models using the Galerkin method but with expansion of arbitrary basis functions. Application of similarity transform to resulting full matrix equations gives rise to a set of uncoupled partial differential equations of which the unknowns are coefficients of mode vector. Using the transform technique a computationally efficient time integration is possible. In present from the model use Chebyshev polynomials for Galerkin solution of internal mode equations. To examine model performance the model is applied to a homogeneous, rectangular basin of constant depth under steady, uniform wind field.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.30 no.6
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• pp.116-123
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• 2002

This paper describes the development of gimbals engine actuation system for KSR(Korean Sounding Rocket)-III which performs the attitude control of pitch and yaw axes by thrust vector control of liquid propellant gimbals engine. The development requirements of configuration, performance and environment are introduced, and the principles and details of components and system development are discussed. The developed system successfully fulfilled its own performance and environmental evaluation. It will be planned to perform verification of interface and integration compatibility with other related systems, and then mounted on KSR-III as a flight hardware system.

• Nuclear Engineering and Technology
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• v.46 no.6
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• pp.725-736
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• 2014

It is very important to detect and identify small anomalies and component failures for the safe operation of complex and large-scale artifacts such as nuclear power plants. Each diagnostic technique has its own advantages and limitations. These facts inspire us not only to enhance the capability of diagnostic techniques but also to integrate the results of diagnostic subsystems in order to obtain more accurate diagnostic results. The article describes the outline of four diagnostic techniques developed for the condition monitoring of the fast breeder reactor "Monju". The techniques are (1) estimation technique of important state variables based on a physical model of the component, (2) a state identification technique by non-linear discrimination function applying SVM (Support Vector Machine), (3) a diagnostic technique applying WT (Wavelet Transformation) to detect changes in the characteristics of measurement signals, and (4) a state identification technique effectively using past cases. In addition, a hybrid diagnostic system in which a final diagnostic result is given by integrating the results from subsystems is introduced, where two sets of values called confidence values and trust values are used. A technique to determine the trust value is investigated under the condition that the confidence value is determined by each subsystem.

• The Journal of Asian Finance, Economics and Business
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• v.7 no.10
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• pp.451-459
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• 2020

This study aims to examine the causality between production input and the price of rice in East Java, Indonesia. This study applied a quantitative method to understand in a comprehensive way the correlation between variables. The data used for this study were collected from several sources, including East Java Agriculture Office, Siskaperbapo.com, and Statistics Indonesia (BPS) of East Java. This research was carried out over five years, starting from 2014 to 2018. Furthermore, the data were analyzed using the Vector Error Correction Model (VECM) by employing E-Views (version 7). The findings of this study indicated that, in the long run, the population, rice production, and changes in people's income have a positive effect on price stability, but are inversely proportional if seen in the short term. In comparison, in the long run, farmer exchange rates variable has a negative impact on price stability, and inversely proportional in the short term, which has a positive effect. There are different implications when the people's income increases and the rice price declines; these have great potential to alleviate poverty in East Java, Indonesia. This is due to the fact that the price stability also concerns the welfare of the community.