• Korean Journal of Animal Reproduction
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• v.25 no.3
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• pp.237-242
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• 2001

We recently demonstrated that both linear- and supercoil-form B1/B2 SINE (short interspersed elements) sequences could increase an integration frequency of a reporter gene in preimplatation mouse embryos. In those reports, when either a control or SINE-flanked DNA was separately applied to microinjection, the proportions of $\beta$-gal positives were 16% and 63%, respectively, in linear-form DNA, and 6% and 25%, respectively, in circular-form DNA. Here, we examined the contribution of a circular-form DNA moiety to integration frequency by using a mixed-farm (linear and circular-form) DNA in microinjection. When examined in the blastocyst stage, the proportion of $\beta$-gal-positive embryos was 17.3% and 46.6% in control and SINE-flanked DNA, respectively. These results suggest that there is little contribution of circular-form DNA moiety to the resultant integration frequency, and that the majority of the integration events are mediated through a linear conformation of vector DNA. In addition, some clues on integration process could be obtained from the analysis of microinjection results.

• The Journal of Asian Finance, Economics and Business
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• v.7 no.12
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• pp.677-682
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• 2020

To investigate the nexus between urban population, gross capital formation, and economic growth in the Kingdom of Saudi Arabia, yearly data was collected from the World Bank for the period 1974- 2018. Basic statistics test and correlation matrix was used to investigate the causal effect among the tested parameters, followed by Augmented Dickey-Fuller (ADF) stationary test, co-integration analysis by Johansen test after that Vector Auto-Correction Model for both short-run and long-run and finally the Granger-Causality tests. Result of unit root test analysis shows that the urban population became stationary at I (0) level while economic growth and gross capital formation became stationary at I (1). Johansen co-integration analysis indicates that there is presence of both long-run and short-run relationship between the three variables in the Kingdom of Saudi Arabia. The result of the VECM Model reflects that both economic growth and gross capital formation have a negative impact on urban population in the short run. According to the Granger-Causality tests, there is unidirectional causality with the urban population by both gross capital formation and economic growth. Also, the result of the Granger Causality tests show that there is unidirectional causality between economic growth and gross capital formations.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.30 no.3
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• pp.8-16
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• 2002

The implicit formulation of rotor dynamics for helicopter flight simulation has been derived and and presented. The generalized vector kinematics regarding the relative motion between coordinates were expressed as a unified matrix operation and applied to get the inertial velocities and accelerations at arbitaty rotor blade span position. Based on these results the rotor aeromechanic equations for flapping dynamics, lead-lag dynamics and torque dynamics were formulated as an implicit form. Spatial integration methods of rotor dynamic equations along blade span and the expanded applicability of the present implicit formulations for arbitrary hings geometry and hinge sequences have been investigated. Time integration methods for present DAE(Differential Algebraic Equation) to calculate dynamic response calculation are recommenaded as future works.

• Journal of Astronomy and Space Sciences
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• v.28 no.1
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• pp.37-54
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• 2011

Two semi-analytic solutions for a perturbed two-body problem known as Lagrange planetary equations (LPE) were compared to a numerical integration of the equation of motion with same perturbation force. To avoid the critical conditions inherited from the configuration of LPE, non-singular orbital elements (EOE) had been introduced. In this study, two types of orbital elements, classical Keplerian orbital elements (COE) and EOE were used for the solution of the LPE. The effectiveness of EOE and the discrepancy between EOE and COE were investigated by using several near critical conditions. The near one revolution, one day, and seven days evolutions of each orbital element described in LPE with COE and EOE were analyzed by comparing it with the directly converted orbital elements from the numerically integrated state vector in Cartesian coordinate. As a result, LPE with EOE has an advantage in long term calculation over LPE with COE in case of relatively small eccentricity.

• Korean Management Science Review
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• v.25 no.3
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• pp.13-26
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• 2008

We investigate the effect of government and public sector R&D Investment at IT Industry on the amount of Production, export and nongovernment R&D Investment at IT Industry. We, firstly, examine the stationarity of each variable by the unit root t-test and perform the co-integration test for the pairs of variables. We use YECM(Vector Error Correction Model) according to the results of co-integration test for the examination of Granger-causality between variables. It is found that there exist an Granger-causality between public sector R&D Investment and nongovernment R&D investment and also between public sector R&D Investment and export. Secondly, we analyze the impulse response of government and public sector R&D Investment at IT Industry on the amount of production, export and nongovernment R&D investment at IT Industry based on VECM model. It is found that the response of the amount of production is highest at 3th period and is lowest at 8th period and that of export shows similar pattern.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.2
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• pp.101-106
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• 2002

This study was conducted to obtain the transformed tobacco (Nicotiana tubacum) plants with cytosolic ascorbate peroxidase gene(ApxSC7) using Agrobacterium tumefaciens LBA4404. A cDNA encoding the cytosolic ascorbate peroxidase of strawberry, ApxSC7, was introduced into tobacco plants via Agrobacterium-mediated gene transfer system. The expression vector, pIG-AP8, harboring ApxSC7 gene was used for production of transgenic tobacco plants. A large number of transgenic plants were regenerated on a medium containing hygromycin. Integration of ApxSC7 gene was confirmed by PCR and Southern blot analyses with genomic DNA. Northern blot analyses revealed that the pIGap8 gene was constitutively expressed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.1
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• pp.21-26
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• 2001

To develop transgenic orchardgrass resistant to reactive oxygen species produced from environmental stresses, a vector with the cytosolic glutathione reductase cDNA (BcGRl) from Chinese cabbage was constructed under the control of the cauliflower mosaic virus 35S promoter and was introduced into orchardgrass using Agrobacterium tumefaciens EHA101. Transgenic plants from hygromycin-selected calli of orchardgrass did not show any morphological difference from wild-type plants. The results of PCR amplification and genomic Southern blot analysis confirmed the integration of foreign gene into the chromosome of transgenic orchardgrass. Northern blot analysis with total RNA from leaves also confirmed the constitutive expression of BcGR1 in transgenic orchardgrass.

• Proceedings of the Korea Institute of Convergence Signal Processing
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• 2006.06a
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• pp.45-48
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• 2006

This paper presents an approach to visual information based temporal indexing of video sequences. The objective of this work is the integration of an automatic face detection and a matching system for video indexing. The face detection is done using color information. The matching stage is based on the Principal Component Analysis (PCA) followed by the Minimax Probability Machine (MPM). Using PCA one feature vector is calculated for each face which is detected at the previous stage from the video sequence and MPM is applied to these feature vectors for matching with the training faces which are manually indexed after extracting from video sequences. The integration of the two stages gives good results. The rate of 86.3% correctly classified frames shows the efficiency of our system.

• The Korean Journal of Mycology
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• v.39 no.3
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• pp.235-238
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• 2011

Agrobacterium harboring vector pCHBs with hygromycin phosphotransferase(hph) and hepatitis B virus surface antigen(HBsAg)gene was transformed into the mycelial culture of Flammulina velutipes. In particular, mild NaOH solution was treated to the mycelia before Agrobacterium infection step. This was purposed to generate putative surface wounds in the mycelial cell walls. The results showed that hygromycin-resistant($hyg^r$) mycelia could be obtained only from NaOH-treated mycelia but not from intact mycelia. The integration of $hyg^r$ gene in fungal genome was confirmed by PCR. In addition, a single transgene integration and heterologous protein expression in F. velutipes could be verified by Southern blot hybridization and western blot analysis, respectively. This study demonstrated an efficient tool for the Agrobacterium-mediated transformation of F. velutipes mycelia.

• Journal of Mechanical Science and Technology
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• v.19 no.12
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• pp.2187-2196
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• 2005

In this paper, the stiffness and the mass matrices for the in-plane motion of a thin circular beam element are derived respectively from the strain energy and the kinetic energy by using the natural shape functions of the exact in-plane displacements which are obtained from an integration of the differential equations of a thin circular beam element in static equilibrium. The matrices are formulated in the local polar coordinate system and in the global Cartesian coordinate system with the effects of shear deformation and rotary inertia. Some numerical examples are performed to verify the element formulation and its analysis capability. The comparison of the FEM results with the theoretical ones shows that the element can describe quite efficiently and accurately the in-plane motion of thin circular beams. The stiffness and the mass matrices with respect to the coefficient vector of shape functions are presented in appendix to be utilized directly in applications without any numerical integration for their formulation.