• 제목/요약/키워드: integration vector

검색결과 300건 처리시간 0.034초

반복 계산법 및 계산 가속기법에 의한 다물체 동역학 해법 (An Accelerated Iterative Method for the Dynamic Analysis of Multibody Systems)

  • 이기수;임철호
    • 대한기계학회논문집
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    • 제16권5호
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    • pp.899-909
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    • 1992
  • 본 연구에서는 대수 미분 방정식을 풀기위한 새로운 방법을 소개한다. 본 작업에서는 Lagrange multiplier의 값이 사전에 주어졌다고 생각하여, 즉 대수 미분 방정식을 순수한 상미분 방정식으로 변환하여, 잘 알려진 시간 적분법을 적용한다. 또 정확한 Lagrange Multiplier값은 반복 계산법(iterative scheme)에 의하여 계산한 다. 시간 적분의 정확도와 제한 조건의 정확도는 모두 보장된다. 특히 제한 조건 의 경우, 위치, 속도 및 가속도의 제한 조건이 모두 만족된다. 또 정확한 Lagrange multiplier의 값을 계산 가속기법(acceleration technique)에 의하여 대단히 빨리 계 산한다. 독립 좌표를 구할 필요가 없으므로 거대한 행열을 decomposition하는 등의 복잡한 절차가 불필요하며 N-R 반복법 역시 불필요하다. 이러한 사항들 및 Jacobian 행열의 sparsity로 인하여 경제적인 계산이 가능하게 된다.

Cloning of Genomic DNAs of Trametes versicolor Acting as Autonomously Replicating Sequences in Saccharomyces cerevisiae

  • Sora An;Park, Kyoung-Phil;Park, Hyoung-Tae;Kim, Kyu-Joong;Kim, Kyunghoon
    • Journal of Microbiology
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    • 제40권3호
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    • pp.245-247
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    • 2002
  • A genomic DNA library of the fungus Trametes versicolor was constructed in a yeast integration vector which contains the URA3 gene of the budding yeast Saccharomyces cerevisiae and the gene responsible for hygromycin B resistance, and fragments acting as autonomously replicating sequences (ARSes) in the budding yeast were identified from the genomic DNA library. Sixteen recombinant plasmids from the library transformed the budding yeast Saccharomyces cerevisiae to Ura+ at high frequencies. They were maintained stably under selective conditions, but were gradually lost from yeast cells at different rates under nonselective conditions, indicating that they contain eukaryotic origins of DNA replication and exist as extrachromosomal plasmids. Base sequences of four ARS DNAs among the 16 cloned fragments revealed that all or the four contain at least one 11 bp [(A/T)TTTA(T/C)(A/G)TTT(A/T)]consensus sequence of the budding yeast ARS.

Production and Secretion of Human Interleukin-18 in Transgenic Tobacco Cell Suspension Culture

  • Sharma, Niti;Kim, Tae-Geum;Yang, Moon-Sik
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제11권2호
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    • pp.154-159
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    • 2006
  • Interleukin-18 (IL-18), otherwise known as interferon-gamma-inducing factor (IGIF), is one of several well characterized and important cytokines that contribute to host defenses. The complementary DNA (cDNA) of mature human interleukin-18 gene (hIL-18) was fused with the signal peptide of the rice amylase 1A gene (Ramy1A) and introduced into the plant expression vector under the control of a duplicated CaMV 35S promoter. The recombinant plasmid was transformed into tobacco (Nicotiana tabacum L. cv Havana) using the Agrobacterium-mediated transformation method. The integration of the hlL-18 gene into the genome of transgenic tobacco plants was confirmed by polymerase chain reaction (PCR) amplification and its expression was observed in the suspension cells that were derived from the transgenic plant callus by using Northern blot analysis. The hlL-18 protein was detected in the extracts of the transgenic callus and in the medium of the transgenic tobacco suspension culture by using immunoblot analysis. Based upon enzyme-linked immunosorbant assay (ELISA) results, the expression level of the hlL-18 protein approximated $166{\mu}g/L$ in the suspension culture medium. Bioassay results from the induction of $interferon-{\gamma}$ from a KG-1 cell line indicated that the hlL-18 secreted into the suspension culture medium was bioactive.

웹 멀티미디어 컨텐츠 서버/클라이언트 통합 시스템의 설계 및 구현 (Design and Implementation of a Integrated web Multimedia Contents Server and Client System)

  • 원덕재;김세영;노강래;신용탁;신동규;신동일
    • 한국정보처리학회:학술대회논문집
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    • 한국정보처리학회 2001년도 춘계학술발표논문집 (하)
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    • pp.611-614
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    • 2001
  • 최근 인터넷 사용의 폭발적인 증가로 인하여 웹 상에서 유기적으로 통합된 멀티미디어 컨텐츠에 대한 서비스 제공의 필요성이 대두되었다. 이에 1998년 W3C(World Wide Web Consortium)는 통합 멀티미디어 표현 언어인 SMIL(Synchronized Multimedia Integration Language)을 발표하였고, SMIL-Boston으로 진행했던 SMIL 1.0 확장 프로젝트를 SVG(Scalable Vector Graphics)의 애니메이션 기능의 도입 및 기능 확장으로 2000년 9월 SMIL 2.0으로 최종 Working Draft안을 발표하게 되었다. 이에 본 논문에서는 SMIL컨텐츠 저작을 위한 자동 생성 모듈 및 전용 저작 도구를 제공하고, SMIL 컨텐츠 데이터베이스 서버 및 재생기와의 통합으로 원활한 SMIL 컨텐츠 서비스 환경을 제공하는 웹 멀티미디어 컨텐츠 서비스를 위한 서버/클라이언트 통합 시스템을 설계 및 구현하였다.

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Efficiency of transformation mediated by Agrobacterium tumefaciens using vacuum infiltration in rice (Oryza sativa L.)

  • Safitri, Fika Ayu;Ubaidillah, Mohammad;Kim, Kyung-Min
    • Journal of Plant Biotechnology
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    • 제43권1호
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    • pp.66-75
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    • 2016
  • Agrobacterium-mediated gene transfer has recently been developed to improve rice transformation. In this study, 3 different transformation methods were tested including soaking, co-cultivation, and vacuum infiltration. Agrobacterium tumefaciens GV3101 harboring the binary vector pGreen:: LeGSNOR was used in this experiment. This study aimed to identify the most appropriate method for transferring LeGSNOR into rice. Vacuum infiltration of the embryonic calli for 5 min in Ilpum resulted in high transformation efficiency based on confirmation by PCR, RT-PCR, and qRT-PCR analyses. In conclusion, we described the development of an efficient transformation protocol for the stable integration of foreign genes into rice; furthermore, the study results confirmed that PCR is suitable for efficient detection of the integrated gene. The vacuum infiltration system is a potentially useful tool for future studies focusing on transferring important genes into rice seed calli, and may help reduce time and effort.

항공사진과 레이져 데이터의 통합에 의한 3 차원 공간정보 활용기술연구 (A Study on the Application Technique of 3-D Spatial Information by integration of Aerial photos and Laser data)

  • 연상호
    • 한국측량학회지
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    • 제28권3호
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    • pp.385-392
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    • 2010
  • A LiDAR technique has the merits that survey engineers can get a large number of measurements with high precision quickly. Aerial photos and satellite sensor images are used for generating 3D spatial images which are matched with the map coordinates and elevation data from digital topographic files. Also, those images are used for matching with 3D spatial image contents through perspective view condition composed along to the designated roads until arrival the corresponding location. Recently, 3D aviation image could be generated by various digital data. The advanced geographical methods for guidance of the destination road are experimented under the GIS environments. More information and access designated are guided by the multimedia contents on internet or from the public tour information desk using the simulation images. The height data based on LiDAR is transformed into DEM, and the real time unification of the vector via digital image mapping and raster via extract evaluation are transformed to trace the generated model of 3-dimensional downtown building along to the long distance for 3D tract model generation.

유사변환기법을 이용한 3차원 모델의 개발 (Developing a Three-dimensional Spectral Model Using Similarity Transform Technique)

  • 강관수;소재귀;정경태;선우중호
    • 한국해안해양공학회지
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    • 제5권2호
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    • pp.107-120
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    • 1993
  • 본 논문은 유사변환기법을 이용한 새로운 3차원 연직 모우드 전개 모델의 개발에 대하여 기술한다. 기본방정식을 External 모우드와 Internal 모우드로 분리시킨 다음 Internal 모우드식에 Galerkin 방법을 적용하고 구성되는 행열방정식에 유사변환기법을 적용, 기저함수의 계수 값을 구하였다. 최종 얻어지는 기저함수의 계수 값은 마찰장을 제외하고는 비연계되어 시간 간격의 제약을 거의 받지않고 연직 구조를 구할 수 있기 때문에 경제성면에서 탁월하다. 수립된 모델은 어떤 기저함수라도 적용 가능하나 현 단계에서는 Chebyshev 다항식함수가 사용되었으며, 바람응력은 일정한 것으로 가정하였다. 모델 테스트로서 정상상태의 균일한 바람응력이 가해지는 장방형 Basin에 적용하여 모델의 적용 가능성을 검증하였다.

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3단형 과학로켓 김발엔진 구동장치 개발 (Development of Gimbals Engine Actuation System for KSR(Korean Sounding Rocket)-III)

  • 민병주;박문수;이희중;최형돈
    • 한국항공우주학회지
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    • 제30권6호
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    • pp.116-123
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    • 2002
  • 본 논문에서는 피치 및 요 방향의 자세제어를 위하여 액체 연료 김발엔진의 추력벡터제어 방식을 사용하는 3단형 과학로켓의 김발엔진 구동장치 개발을 기술하였다. 김발엔진 구동장치 개발에 필요한 형상 및 성능, 환경 요구조건을 소개하였으며, 부품 및 시스템 개발원리 및 세부 사항을 설명하였다. 개발된 시스템에 대한 자체 성능 및 환경요구조건 평가를 성공적으로 완료하였다. 구동장치와 연계된 타 시스템과의 접속 및 통합 적합성 검증 후, 비행용 하드웨어 시스템으로 KSR-III 로켓에 탑재될 예정이다.

INTEGRATED DIAGNOSTIC TECHNIQUE FOR NUCLEAR POWER PLANTS

  • Gofuku, Akio
    • Nuclear Engineering and Technology
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    • 제46권6호
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    • pp.725-736
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    • 2014
  • It is very important to detect and identify small anomalies and component failures for the safe operation of complex and large-scale artifacts such as nuclear power plants. Each diagnostic technique has its own advantages and limitations. These facts inspire us not only to enhance the capability of diagnostic techniques but also to integrate the results of diagnostic subsystems in order to obtain more accurate diagnostic results. The article describes the outline of four diagnostic techniques developed for the condition monitoring of the fast breeder reactor "Monju". The techniques are (1) estimation technique of important state variables based on a physical model of the component, (2) a state identification technique by non-linear discrimination function applying SVM (Support Vector Machine), (3) a diagnostic technique applying WT (Wavelet Transformation) to detect changes in the characteristics of measurement signals, and (4) a state identification technique effectively using past cases. In addition, a hybrid diagnostic system in which a final diagnostic result is given by integrating the results from subsystems is introduced, where two sets of values called confidence values and trust values are used. A technique to determine the trust value is investigated under the condition that the confidence value is determined by each subsystem.

Nexus between Production Input and Price Commodity: An Integration Analysis of Rice Barns in East Java of Indonesia

  • WULANDARI, Dwi;NARMADITYA, Bagus Shandy;PRAYITNO, Putra Hilmi;ISHAK, Suryati;SAHID, Sheerad;QODRI, Lutfi Asnan
    • The Journal of Asian Finance, Economics and Business
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    • 제7권10호
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    • pp.451-459
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    • 2020
  • This study aims to examine the causality between production input and the price of rice in East Java, Indonesia. This study applied a quantitative method to understand in a comprehensive way the correlation between variables. The data used for this study were collected from several sources, including East Java Agriculture Office, Siskaperbapo.com, and Statistics Indonesia (BPS) of East Java. This research was carried out over five years, starting from 2014 to 2018. Furthermore, the data were analyzed using the Vector Error Correction Model (VECM) by employing E-Views (version 7). The findings of this study indicated that, in the long run, the population, rice production, and changes in people's income have a positive effect on price stability, but are inversely proportional if seen in the short term. In comparison, in the long run, farmer exchange rates variable has a negative impact on price stability, and inversely proportional in the short term, which has a positive effect. There are different implications when the people's income increases and the rice price declines; these have great potential to alleviate poverty in East Java, Indonesia. This is due to the fact that the price stability also concerns the welfare of the community.