• Food Science and Biotechnology
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• v.15 no.4
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• pp.485-493
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• 2006

Lactococcus species are noninvasive and nonpathogenic microorganisms that are widely used in industrial food fermentation and as well-known probiotics. They have been modified by traditional methods and genetic engineering to produce useful food-grade materials. The application of genetically modified lactococci in the food industry requires their genetic elements to be safe and stable from integration with endogenous food microorganisms. In addition, selection for antibiotic-resistance genes should be avoided. Several expression and secretion signals have been developed for the production and secretion of useful proteins in lactococci. Food-grade systems composed of genetic elements from lactic acid bacteria have been developed. Recent developments in this area have focused on food-grade selection markers, stabilization, and integration strategies, as well as approaches for controlled gene expression and secretion of foreign proteins. This paper reviews the expression and secretion signals available in lactococci and the development of food-grade markers, food-grade cloning vectors, and integrative food-grade systems.

• The Transactions of the Korean Institute of Power Electronics
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• v.26 no.6
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• pp.381-389
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• 2021

Most diagnosis approaches rely on historical failure data that might not be feasible in real operating conditions because the battery voltage and internal parameters are nonlinear according to various operating conditions, such as cell-to-cell configuration and initial condition. To overcome this issue, the estimator and the predictor require integrated approaches that consider comprehensive data, with the degradation process and measured data taken into account. In this paper, vector autoregressive models (VAR) with various parameters that affect overdischarge to the cell in the battery pack were constructed, and the cell-to-cell parameters were identified using an adaptive model to analyze the influence of failure prognosis. The theoretical analysis is validated using experimental results in terms of the feasibility and advantages of fault prognosis.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.34 no.12
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• pp.82-89
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• 2006

This paper describes the development results of electrohydraulic actuation system which performs the attitude and trajectory control of pitch and yaw motion using thrust vector control for high altitude launching vehicle operated in high vacuum environment (altitude higher than 300 km). As compared with electrohydraulic actuation system for low altitude launch vehicle which operated under altitude of stratosphere, the intensified development requirements, newly adopted design and manufacturing technologies, newly developed test equipments and test results are summarized in this paper. The development test and evaluation of actuation system were successfully accomplished. The developed actuation system will be installed on KSLV-I after finishing verification of interface and integration compatibility with related other systems.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.107-113
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• 1997

Transformants of White Clover(Trifolium repens L.) were efficiently produced from immature seed derived callus cocultivated with Agrobacterium twnefaciens LBA4404 harboring plant binary vector. pBI121, using acetosyringone. The mean frequencies of transformants on the two kanamycin-containing media were 16 to 19% when the immature seed-derived calli were infected with bacteria cultured in the presence of 100$\mu$M acetosyringone compared with 7% in media without acetosyringone. Transgenic white clover was subject to molecular analysis for integration into plant nuclear genome and expression of $\beta$-glucuronidase(GUS) gene. PCR and Northern blot analyses demonstrated that GUS gene was integrated into white clover nuclear genome and expressed into its mRNA. The expression of GUS gene into its protein was confirmed by spectrophotometric assay of GUS activity.

• Korean Journal of Plant Tissue Culture
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• v.21 no.1
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• pp.55-58
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• 1994

Agrobacterium tumefaciens LABA4404 harboring plant binary vector, pBI121, was used for genetic transformation of lettuce (Lactuca sativa t.). Cotyledon segments were infected with A. tumefaciens LBA4404 by cocultivation method and regenerated. Regenerated letture was subject to molecular analyses for integration into plant nuclear genome and expression of ${\beta}$-glucumnidase (GUS) gene. Southern and Northern blot analyses demonstrated that GUS gene was integrated into plant nuclear genome and expressed into its mRNA. The expression of GUS gene into its protein was confirmed by specetrophotometric assay of GUS activity.

• The Korean Journal of Applied Statistics
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• v.17 no.2
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• pp.239-252
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• 2004

It is known that the domestic and the U.S. stock prices tend to move together as those markets are closely interrelated. In this study, cointegration and causal relationships among the four stock price indexes of KOSPI, KOSDAQ, DOWJONES and NASDAQ are carefully investigated for the period of declining stock prices in the long run. When all indexes move in a similar fashion, cointegration does not exist and the causal linkages between the domestic and the U.S. stock prices appear relatively complex. On the other hand, when the domestic and the V.S. stock prices move in a different manner, cointegration exists and the causal relationships appear relatively simple. NASDAQ is apparently found to lead the domestic stock market in both periods, which is consistent with the actual market situation when the If industry is under recession.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2008.10a
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• pp.100-103
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• 2008

In this paper, we propose H.264 Encoder integrating Intra Prediction, Deblocking filter, Context-Based Adaptive Variable Length Coding, and Motion Estimation encoder module. This designed module can be operated in 440 cycle for one-macroblock. To verify the Encoder architecture, we developed the reference C from JM 9.4 and verified the our developed hardware using test vector generated by reference C. The designed circuit can be operated in 166MHz clock system, and has 1800k gate counts using Charterd 0.18um process including SRAM memory. Manufactured chip has the size of $6{\times}6mm$ and 208 pins package.

• Journal of Information Processing Systems
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• v.16 no.2
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• pp.329-342
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• 2020

In recent years, emotional text classification is one of the essential research contents in the field of natural language processing. It has been widely used in the sentiment analysis of commodities like hotels, and other commentary corpus. This paper proposes an improved W-LDA (weighted latent Dirichlet allocation) topic model to improve the shortcomings of traditional LDA topic models. In the process of the topic of word sampling and its word distribution expectation calculation of the Gibbs of the W-LDA topic model. An average weighted value is adopted to avoid topic-related words from being submerged by high-frequency words, to improve the distinction of the topic. It further integrates the highest classification of the algorithm of support vector machine based on the extracted high-quality document-topic distribution and topic-word vectors. Finally, an efficient integration method is constructed for the analysis and extraction of emotional words, topic distribution calculations, and sentiment classification. Through tests on real teaching evaluation data and test set of public comment set, the results show that the method proposed in the paper has distinct advantages compared with other two typical algorithms in terms of subject differentiation, classification precision, and F1-measure.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2003.11a
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• pp.465-470
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• 2003

In this study, the state-space Newmark method based on average velocity is presented to analyse the transient dynamic response for general dynamic system. The conventional Newmark method based on average acceleration cannot he directly to the first-order state-space differential equations introducing the state-space vector. To overcome this problem, the time-step integration algorithm, based on average velocity concept, suitable for the first-order state-space differential equations is proposed In results, the proposed method has %he numerical stability and order of accuracy, which is proved analytically, equal to those of the conventional Newmark method based on average acceleration. Also, the formulation for numerical solution is very simple and the calculation time Is nearly equal to that of the conventional Newmark method based on average acceleration in spite of an increase of two times over matrix size. This method will be look forward to applying the general dynamic system to calculate the transient dynamic response.

• The Journal of Korea Robotics Society
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• v.9 no.1
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• pp.1-10
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• 2014

In recent years, the research of 3D mapping technique in urban environments obtained by mobile robots equipped with multiple sensors for recognizing the robot's surroundings is being studied actively. However, the map generated by simple integration of multiple sensors data only gives spatial information to robots. To get a semantic knowledge to help an autonomous mobile robot from the map, the robot has to convert low-level map representations to higher-level ones containing semantic knowledge of a scene. Given a 3D point cloud of an urban scene, this research proposes a method to recognize the objects effectively using 3D graph model for autonomous mobile robots. The proposed method is decomposed into three steps: sequential range data acquisition, normal vector estimation and incremental graph-based segmentation. This method guarantees the both real-time performance and accuracy of recognizing the objects in real urban environments. Also, it can provide plentiful data for classifying the objects. To evaluate a performance of proposed method, computation time and recognition rate of objects are analyzed. Experimental results show that the proposed method has efficiently in understanding the semantic knowledge of an urban environment.