• Ocean Systems Engineering
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• v.6 no.3
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• pp.265-287
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• 2016

The change of the global performance of a turret-moored FPSO (Floating Production Storage Offloading) with DP (Dynamic Positioning) control is simulated, analyzed, and compared for two different internal turret location cases; bow and midship. Both collinear and non-collinear 100-yr GOM (Gulf of Mexico) storm environments and three cases (mooring-only, with DP position control, with DP position+heading control) are considered. The horizontal trajectory, 6DOF (degree of freedom) motions, fairlead mooring and riser tension, and fuel consumptions are compared. The PID (Proportional-Integral-Derivative) controller based on LQR (linear quadratic regulator) theory and the thrust-allocation algorithm which is based on the penalty optimization theory are implemented in the fully-coupled time-domain hull-mooring-riser-DP simulation program. Both in collinear and non-collinear 100-yr WWC (wind-wave-current) environments, the advantage of mid-ship turret is demonstrated by the significant reduction in heave at the turret location due to the minimal coupling with pitch mode, which is beneficial to mooring and riser design. However, in the non-collinear WWC environment, the mid-turret case exhibits unfavorable weathervaning characteristics, which can be reduced by employing DP position and heading controls as demonstrated in the present case studies. The present study also reveals the plausible cause of the failure of mid-turret Gryphon Alpha FPSO in milder environment than its survival condition.

• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1744-1754
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• 2014

The hepatitis C virus (HCV) RNA genome is replicated by an RNA replicase complex (RC) consisting of cellular proteins and viral nonstructural (NS) proteins, including NS5B, an RNA-dependent RNA polymerase (RdRp) and key enzyme for viral RNA genome replication. The HCV RC is known to be associated with an intracellular membrane structure, but the cellular components of the RC and their roles in the formation of the HCV RC have not been well characterized. In this study, we took a proteomic approach to identify stomatin, a member of the integral proteins of lipid rafts, as a cellular protein interacting with HCV NS5B. Co-immunoprecipitation and co-localization studies confirmed the interaction between stomatin and NS5B. We demonstrated that the subcellular fraction containing viral NS proteins and stomatin displays RdRp activity. Membrane flotation assays with the HCV genome replication-competent subcellular fraction revealed that the HCV RdRp and stomatin are associated with the lipid raft-like domain of membranous structures. Stomatin silencing by RNA interference led to the release of NS5B from the detergent-resistant membrane, thereby inhibiting HCV replication in both HCV subgenomic replicon-harboring cells and HCV-infected cells. Our results identify stomatin as a cellular protein that plays a role in the formation of an enzymatically active HCV RC on a detergent-resistant membrane structure.

• Mycobiology
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• v.42 no.2
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• pp.167-173
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• 2014

A ${\beta}$-glucan synthase gene was isolated from the genomic DNA of polypore mushroom Sparassis crispa, which reportedly produces unusually high amount of soluble ${\beta}$-1,3-glucan (${\beta}$-glucan). Sequencing and subsequent open reading frame analysis of the isolated gene revealed that the gene (5,502 bp) consisted of 10 exons separated by nine introns. The predicted mRNA encoded a ${\beta}$-glucan synthase protein, consisting of 1,576 amino acid residues. Comparison of the predicted protein sequence with multiple fungal ${\beta}$-glucan synthases estimated that the isolated gene contained a complete N-terminus but was lacking approximately 70 amino acid residues in the C-terminus. Fungal ${\beta}$-glucan synthases are integral membrane proteins, containing the two catalytic and two transmembrane domains. The lacking C-terminal part of S. crispa ${\beta}$-glucan synthase was estimated to include catalytically insignificant transmembrane ${\alpha}$-helices and loops. Sequence analysis of 101 fungal ${\beta}$-glucan synthases, obtained from public databases, revealed that the ${\beta}$-glucan synthases with various fungal origins were categorized into corresponding fungal groups in the classification system. Interestingly, mushrooms belonging to the class Agaricomycetes were found to contain two distinct types (Type I and II) of ${\beta}$-glucan synthases with the type-specific sequence signatures in the loop regions. S. crispa ${\beta}$-glucan synthase in this study belonged to Type II family, meaning Type I ${\beta}$-glucan synthase is expected to be discovered in S. crispa. The high productivity of soluble ${\beta}$-glucan was not explained but detailed biochemical studies on the catalytic loop domain in the S. crispa ${\beta}$-glucan synthase will provide better explanations.

• Journal of Institute of Control, Robotics and Systems
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• v.18 no.6
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• pp.546-554
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• 2012

This paper suggests a design method of an improved phase control loop for tracking resonant frequency of solid type precision resonant gyroscope. In general, a low cost MEMS gyroscope adapts the automatic gain control loops by taking a velocity feedback configuration. This control technique for controlling the resonance amplitude shows a stable performance. But in terms of resonant frequency tracking, this technique shows an unreliable performance due to phase errors because the AGC method cannot provide an active phase control capability. For the resonance control loop design of a solid type precision resonant gyroscope, this paper presents a phase domain control loop based on linear PLL (Phase Locked Loop). In particular, phase control loop is exploited using a higher order PLL loop filter by extending the first order active PI (Proportion-Integral) filter. For the verification of the proposed loop design, a hemispherical resonant gyroscope is considered. Numerical simulation result demonstrates that the control loop shows a robust performance against initial resonant frequency gap between resonator and voltage control oscillator. Also it is verified that the designed loop achieves a stable oscillation even under the initial frequency gap condition of about 25 Hz, which amounts to about 1% of the natural frequency of a conventional resonant gyroscope.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.5
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• pp.1033-1042
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• 1994

Dynamic behavior of flexible rectangular liquid containers is analyzed by a two-dimensional coupled boundary element-finite element method. The irrotational motion of inviscid and incompressible ideal fluid is modeled by boundary elements and the motion of structure by finite elements. A singularity free integral formulation is employed for the implementation of boundary element method. Coupling is performed by using compatibility and equilibrium conditions along the interface between the fluid and structure. The fluid-structure interaction effects are reflected into the coupled equation of motion as added fluid mass matrix and sloshing stiffness matrix. By solving the eigen-problem for the coupled equation of motion, natural frequencies and mode shapes of coupled system are obtained. The free surface sloshing motion and hydrodynamic pressure developed in a flexible rectangular container due to horizontal and vertical ground motions are computed in time domain.

• Transactions of the Korean Society of Mechanical Engineers
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• v.14 no.6
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• pp.1408-1416
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• 1990

A powerful and efficient method for finding approximate solutions to initial-boundary-value problems in the transient coupled thermoelasticity is formulated in time domain using the finite element technique with time-marching strategy. The final system equations can be derived by the Guritin's variational principle using the definition of convolution integral. But, the finite element formulation for the equations of motion is modified by differentiating in time. Numerical results to some test problems are compared with analytical and other sophisticated approximate solutions. Stable responces are observed in all the given examples irrespective of incremental time steps and mesh shapes. In addition, it is shown that good numerical results are obtained even in coarser mesh or larger time step comparing to other numerical methods.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.3
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• pp.419-428
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• 2000

Multiconductor transmission line(MTL) equations are solved by FDTD(Finite-Difference Time-Domain) method to predict crosstalk and fields to transmission line coupling on lossy multiconductor transmission lines terminated in arbitrary linear loads. Skin effect losses as well as dc losses are included in the analysis. In order to increase computational efficiency, the convolution integral of internal impedance of conductors and the line currents is computed by using Prony method. For boundary conditions of MTLs terminated in linear loads, state-variable formulation is adopted. The simulated results by FDTD method are compared with the measured ones obtained by using TEM cell. The predictions are in good agreement with the measurements. In addition, it has been found that skin effect losses as well as dc losses of the conductors should be included for accurate predictions on relatively high loss transmission lines such as PCB. It has also been found that dc losses and skin-effect losses affect late-time responses and early-time responses, respectively.

• Journal of Drive and Control
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• v.15 no.1
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• pp.28-37
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• 2018

When the wind speed rises above the rated wind speed, the produced power of the wind turbines exceeds the rated power. Even more, the excessive power results in the undesirable mechanical load and fatigue. A solution to this problem is pitch control of the wind turbines. This paper presents a systematic design method of a collective pitch controller for the wind turbines using a discrete fuzzy Proportional-Integral (PI) controller. Unlike conventional PI controllers, the fuzzy PI controller has variable gains according to its input variables. Generally, tuning the parameters of fuzzy PI controller is complex due to the presence of too many parameters strongly coupled. In this paper, a systematic method for the fuzzy PI controller is presented. First, we show the fact that the fuzzy PI controller is a superset of the PI controller in the discrete-time domain and the initial parameters of the fuzzy PI controller is selected by using this relationship. Second, for simplicity of the design, we use only four rules to construct nonlinear fuzzy control surface. The tuning parameters of the proposed fuzzy PI controller are also obtained by the aforementioned relationship between the PI controller and the fuzzy PI controller. As a result, unlike the PI controller, the proposed fuzzy PI controller has variable gains which allow the pitch control system to operate in broader operating regions. The effectiveness of the proposed controller is verified with computer simulations using FAST, a NREL's primary computer-aided engineering tool for horizontal axis wind turbines.

• Journal of the Korean Society for Marine Environment & Energy
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• v.10 no.3
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• pp.173-180
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• 2007

Oscillating wave amplitude in a bottom-mounted OWC chamber designed for wave energy converter is investigated by applying characteristic wave conditions in Korean coastal water. The effects of shape parameters of OWC chamber in a view of wave energy absorbing capability are analyzed. Both experimental and numerical approaches are adopted and their results are compared to optimize the shape parameters which can result in a maximum power production under given wave distribution. The experiment was carried out in a wave flume under 2-D assumption of OWC chamber. The numerical scheme employed a hybrid Green integral equation which adopts the Rankine Green function inside chamber to take account of fluctuating air pressure, while it uses the Kelvin Green function in outer domain. Air duct diameter, chamber width, and submerged depths of front skirt and back wall of chamber changes the magnitude and peak frequency of wave absorption significantly.

• Journal of IKEEE
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• v.24 no.1
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• pp.154-160
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• 2020

This paper presents a grid current control algorithm for a grid connected inverter (GCI) system in a stationary reference frame. When a Proportional Integral (PI) controller at a stationary reference frame is used in a GCI system, steady state error and phase lags are presented because AC signals are controlled at a stationary reference frame. In this paper, a feedforward controller is applied to the PI controller to compensate the steady state error and phase lags by improving command tracking performance. In addition, disturbance rejection control is applied to the PI controller to protect the GCI system by eliminating disturbance, grid voltage in a GCI system, when a grid fault such as line-to-line fault, happens. The proposed GCI current control algorithm is analyzed in a frequency domain and a simulation model of the proposed GCI current control system is developed for verification of the performance.