• 미생물학회지
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• 제22권1호
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• pp.49-56
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• 1984

Novozym 234에 의한 K. fragilis와 C. pseudotropicalis의 원형질체 형성과 재생에 관한 연구를 하였다. intact cell을 원형질체로 전환시키는데 정지기의 세포보다 대수기에 있는세포가 효과적이었으며 osmotic stabilizer로는 $(NH_4)_{2}SO_4$가 두 종 똑같이 원형질체 형성이나 재생에 있어서 적합했고 최적 효소농도는 K. Fragilis에서는 3mg/ml, C. pseudotropicalis에서는 1~3mg/ml이었다. 반응 후 관찰된 세포 중의 원형질체 형성 수율은 K. fragilis에서 약 95%, C. pseudotropicalis에서 약 100%였으나 원형질체의 재생은 Glusulase와 비교해 볼 때 매우 낮았다. 그러나 세포 이물질이 없는 원형질체를 얻는데는 Novozym 234가 적합한 것 같다.

• 한국미생물·생명공학회지
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• 제17권1호
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• pp.29-34
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• 1989

고도 카드뮴 내성 효모, Hansenula anomala B-7 의 intact cell에 의한 카드뮴의 세포내 축적에 미치는 계면활성제의 영향를 검토하고, Triton X-100의 존재하에서의 카드뮴 축적에 미치는 제인자 등을 검토했다. 카드뮴의 축적은 0.1% Triton X-100과 Aerosol OT에 의하여 약 40% 이상 증가되었다. 카드뮴 이온에 대한 Km값은 0.247mM로 계산되었다. 카드뮴의 축적 최적 pH는 pH7.0에서 pH10.0 사이였으며, 최적온도는 4$0^{\circ}C$였다. 카드뮴의 최적 축적은 정치보다 진탕하므로 약 3배 증가되었으며, 진탕속도는 영향을 미치지 않았다. 카드뮴과 아연 이온을 공존시키므로 카드뮴의 축적이 아연 이온에 의하여 저해되었다.

• KSBB Journal
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• 제27권6호
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• pp.367-374
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• 2012

Cigarette smoking (SM) is considered to be well known environmental toxin which contributes to the onset of various diseases. SM cause direct lungs damage, activate lungs inflammatory responses, and in some cases leads to the development of lung cancer. Cytokines in injured starfish (Asterina pectinifera) is the potential changes in its expression during the regeneration process. Especially, expression of TGF-${\beta}1$ has increased in arm cut starfish extract after eight days. Also, starfish including saponin like the ginseng. Saponin is widely used in the world because of some effective pharmacological activities. Therefore, the current study was designed to elucidate the pharmacological activities of starfish extract against cigarette smoking induced damage in cell line and pulmonary tissue. We investigate that the effect of eight days starfish extract after arm cut (8d) and intact starfish extract on cell line and mouse lung injury by SM. In cell proliferation analysis, although cigarette smoking extract (CSE) was co-treated, the higher proliferation ability is shown in 8d treatment than intact starfish extract. 8d and intact starfish extract was directly transported to pulmonary cells through respiratory organ by nebulizer inhalation. In this case of cigarette smoking, the pulmonary structure was damaged and functions become abnormal. However, 8d treated groups showed similar with the control group compared with SM group. Among them, 8d was proved to be more effective than intact starfish extract. These results demonstrate that 8d could more protect pulmonary structure and function than intact starfish extract against cigarette smoking by ginseng like saponin and regulation of inflammatory cytokines.

• Archives of Pharmacal Research
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• 제10권4호
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• pp.212-218
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• 1987

Intact brain cell aggregates were dissociated from adult rat brains without cerebellum using a sieving technique. This proparation was used to elucidate the binding characteristics of agonist to muscarinic acetylcholine receptors (mAchR) in brain. Incubation of cells with carbamylcholine (carbachol) was shown agonist-induced receptor down-regulation depending on the concentration of agonist, not depending on the incubation time. This effect of carbachol was due to a reduction in the maximal binding capacity ($B_{max}$) to the mAchR without decreasing the affinity of the remaining receptors in incubation at 37.deg.C but was not apparent inincubation at $15^{\circ}}C$In addition, it was abolished when the receptors were blocked by atropine. The decline in ($^3H$)N-methylscopolamine (($^3H$)NMS) binding induced by agonist was reflected as a significant reduction in the receptor density with no change in receptor affinity, suggesting that 'true' receptor down-regulation takes place. Moreover, when the receptors were labeled with the lipophilic antagonist ($^3H$) quinuclidinyl benzilate (($^3H$) QNB) insted of the hydrophilic ligand ($^3H$)NMS, the magnitude of the observed receptor down-regulation was significantly lower in case of the former than the latter. This suggested that exposure of intact brain cells to muscarinic agonists might induce a slight degree of accumulation of receptors in intracellular sites before the receptors are actually degraded.

• Toxicological Research
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• 제25권3호
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• pp.119-124
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• 2009

Genes related to Mycoplasma hyopneumoniae-induced inflammation were identified using the genefishing technology, an improved method for identifying differentially expressed genes (DEGs) using an annealing control primer (ACP) system in RAW264.7 cells. After treatment with M. hyopneumoniae, 16 DEGs were expressed in RAW264.7 cells using a pre-screening system. Among these 16 DEGs, 11 DEGs (DEGs 1, 4, 5-10, 12-15) were selected and sequenced directly, revealing that DEG12 (Grap), DEG14 (Gadd45), and DEG15 (secreted phosphoprotein 1) were related to inflammatory cytokines. This is the first report that intact M. hyopneumoniae induces the expression of Grap, Gadd 45${\beta}$, and secreted phosphoprotein 1 in RAW264.7 cells. Subsequently, these genes may be targets for screening novel inhibitors of the mycoplasmal inflammatory response.

• BMB Reports
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• 제46권2호
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• pp.65-72
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• 2013

In situ detection of RNAs is becoming increasingly important for analysis of gene expression within and between intact cells in tissues. International genomics efforts are now cataloging patterns of RNA transcription that play roles in cell function, differentiation, and disease formation, and they are demon-strating the importance of coding and noncoding RNA transcripts in these processes. However, these techniques typically provide ensemble averages of transcription across many cells. In situ hybridization-based analysis methods complement these studies by providing information about how expression levels change between cells within normal and diseased tissues, and they provide information about the localization of transcripts within cells, which is important in understanding mechanisms of gene regulation. Multi-color, single-molecule fluorescence in situ hybridization (smFISH) is particularly useful since it enables analysis of several different transcripts simultaneously. Combining smFISH with immunofluorescent protein detection provides additional information about the association between transcription level, cellular localization, and protein expression in individual cells.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2002년도 추계학술대회초록집
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• pp.144-144
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• 2002

Muscular lesion was detected in a 23-month-old castrated bull encountered at Kyungsan slaughter house. The lesion appeared as fat intervening muscle fibers. The affected animal had no clinical signs. On microscopic examination, there was replacement of many muscle fibers by normal fat cells. Numerous fat cells were located between muscle fibers. Remaining skeletal muscle cells were in degenerative process, and thus abnormal skeletal muscle cells had loose fibers while normal had intact ones. The advent of inflammatory cells is not at the lesion, which is unique view in steatosis.

• 한국발생생물학회지:발생과생식
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• 제17권2호
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• pp.141-147
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• 2013

Keeping the intact germinal vesicle (GV) is essential for maintaining the capacity of mammals including human. It is maintained by very complex procedures along with folliculogenesis and is a critical step for getting competent oocyte. So far, a few mechanisms involved in folliculogenesis are known but GV arrest mechanisms are largely unrevealed. Cyclic AMP, a adenosine derived substance, have been used as inhibitor of germinal vesicle breakdown as a putative oocyte maturation inhibitor. In this study, we examined the potency of adenosine as GV maintainer and a possible signaling mediator for that. A1, A2b, and A3 were detected in cumulus cells of cumulus enclosed-oocyte (CEO). Intact of germinal vesicle was not kept like in follicle but the spontaneous maturation was inhibited by exogenous adenosine. It is inhibited with concentration dependent manners. Intracellular calcium level of cumulus was extensively increased after adenosine treatment. Based on these results it is suggested that one of the pathway for GV arrest by adenosine and its receptors is calcium mediated signaling pathway in CEO.

• 미생물학회지
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• 제22권4호
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• pp.215-222
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• 1984

Rhodotorula gl$\varkappa$tinis 세포벽에 작용하는 용해 효소 생산곰팡이를 토양으로부터 분리하였고, Aspergillus f'||'&'||'micro;mig$\alpha$tus에 속하는 species로 동정되었다. 이 세포벽 용해효소는 세표외 유도효소였으며 lytic polysaccharidase 와 protease로 구성되어 생세포 용해에 공동으로 착용하였다. 이 lytic polysaccharidase는 Ascomycetous 효모에서의 주 구성 결합인 ${\beta}-1,3-$와 ${\beta}-1$, 6-glucan에는 작용치 않았다. 이 효소는 생세포에는 역가가 낮았지만 R. glutinis의 분획된 세포액에는 protease의 도움없이 작용할 수 있었다.

• Archives of Reconstructive Microsurgery
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• 제2권1호
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• pp.77-81
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• 1993

Free tissue transplantation is commonly performed with the brilliant achievement in microsurgery and anticoagulants and antithrombotic agents have been prescribed in the procedures. However, there is no clean-cut indication as to which agents would be more effective in every steps and final consequences. Low molecular weight heparins inhibiting coagulation in plateletrich plasma and acting on the vascular endothelium have antithrombotic and fibrinolysis action. The experiment with rat groin free flap transplantation after 6-hour ischemia and injection of the low molecular weight heparin was performed and the results between the injection and non-injection group were analysed as follows, 1. Both of the 24-hour groups, vessel patency was not proportional to color change of the groin flap. 2. On the second day after anastomois, heparin-injection group showed intact intima, patent lumen without thrombus, and mild granulomatous inflammation around the suture material and control group with doubtful patency revealed intimal loss and thrombus formation. 3. On the 5th, 7th, and 9th postoperative day, heparin group was patent in anastomosis and showed acute inflammatory cells. 4. The 7th-week period, heparin-injection group showed intact flap color, patent lumen with intact intima and persistent foreign body granuloma.