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http://dx.doi.org/10.5487/TR.2009.25.3.119

Mycoplasma hyopneumoniae Induces Grap, Gadd45β, and secreted phosphoprotein 1 Gene Expression as Part of the Inflammatory Response in RAW264.7 Cells  

Hwang, Mi-Hyun (College of Veterinary Medicine, Kyungpook National University)
Choi, Myung-Jin (College of Veterinary Medicine, Kyungpook National University)
Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Toxicological Research / v.25, no.3, 2009 , pp. 119-124 More about this Journal
Abstract
Genes related to Mycoplasma hyopneumoniae-induced inflammation were identified using the genefishing technology, an improved method for identifying differentially expressed genes (DEGs) using an annealing control primer (ACP) system in RAW264.7 cells. After treatment with M. hyopneumoniae, 16 DEGs were expressed in RAW264.7 cells using a pre-screening system. Among these 16 DEGs, 11 DEGs (DEGs 1, 4, 5-10, 12-15) were selected and sequenced directly, revealing that DEG12 (Grap), DEG14 (Gadd45), and DEG15 (secreted phosphoprotein 1) were related to inflammatory cytokines. This is the first report that intact M. hyopneumoniae induces the expression of Grap, Gadd 45${\beta}$, and secreted phosphoprotein 1 in RAW264.7 cells. Subsequently, these genes may be targets for screening novel inhibitors of the mycoplasmal inflammatory response.
Keywords
Genefishing; Mycoplasma hyopneumoniae; Mycoplasmal inflammatory response; RAW264.7 cells;
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