• Wind and Structures
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• 제17권3호
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• pp.317-343
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• 2013

When evaluating flutter instability, it is often assumed that incident wind is normal to the longitudinal axis of a bridge and the flutter critical wind speed estimated from this direction is most unfavorable. However, the results obtained in this study via oblique sectional model tests of four typical types of bridge decks show that the lowest flutter critical wind speeds often occur in the yaw wind cases. The four types of bridge decks tested include a flat single-box deck, a flat ${\Pi}$-shaped thin-wall deck, a flat twin side-girder deck, and a truss-stiffened deck with and without a narrow central gap. The yaw wind effect could reduce the critical wind speed by about 6%, 2%, 8%, 7%, respectively, for the above four types of decks within a wind inclination angle range between $-3^{\circ}$ and $3^{\circ}$, and the yaw wind angles corresponding to the minimal critical wind speeds are between $4^{\circ}$ and $15^{\circ}$. It was also found that the flutter critical wind speed varies in an undulate manner with the increase of yaw angle, and the variation pattern is largely dependent on both deck shape and wind inclination angle. Therefore, the cosine rule based on the mean wind decomposition is generally inapplicable to the estimation of flutter critical wind speed of long-span bridges under skew winds. The unfavorable effect of yaw wind on the flutter instability of long-span bridges should be taken into consideration seriously in the future practice, especially for supper-long span bridges in strong wind regions.

• 한국공간정보시스템학회:학술대회논문집
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• 한국공간정보시스템학회 2004년도 춘계 학술발표회 논문집 제1권1호(통권1호)
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• pp.75-82
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• 2004

Many researcher's efforts have made a significant advancement of space frame structure with various portion, and it becomes the most outsanding one of space structures. However, with the characteristics of thin and long term of spacing, the unstable behavior of space structure is shown by initial imperfection, erection procedure or joint, especially space frame structure represents more. This kind of unstable problem could not be set up clearly and there is a huge difference between theory and experiment. Moreover, the discrete structure such as space frame has more complex solution, this it is not easy to derive the formulation of design about space structure. In this space frame structure, the character of rise-span ratio or load mode is represented by the instability of space frame structure with initial imperfection, and snap-through or bifurcation might be the main phenomenon. Therefore, in this study, space frame structure which has a lot of aesthetic effect and profitable for large space covering single layer is dealt. And because that the unstable behavior due to variation of inner force resistance in the elastic range is very important collapse mechanism, I would like to investigate unstable character as a nonlinear behavior with a geometric nonlinear. In order to study the instability. I derive tangent stiffness matrix using finite element method and with displacement incremental method perform nonlinear analysis of unit space structure, star dome and 3-ring star dome considering rise-span $ratio(\mu}$ and load $ratio(R_L)$ for analyzing unstable phenomenon.

• Journal of Microbiology and Biotechnology
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• 제32권11호
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• pp.1471-1478
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• 2022

2'-Fucosyllactose (2'-FL), the most abundant fucosylated oligosaccharide in human milk, has multiple beneficial effects on human health. However, its biosynthesis by metabolically engineered Escherichia coli is often hampered owing to the insolubility and instability of α-1,2-fucosyltransferase (the rate-limiting enzyme). In this study, we aimed to enhance 2'-FL production by increasing the expression of soluble α-1,2-fucosyltransferase from Helicobacter pylori (FucT2). Because structural information regarding FucT2 has not been unveiled, we decided to improve the expression of soluble FucT2 in E. coli via directed evolution using a protein solubility biosensor that links protein solubility to antimicrobial resistance. For such a system to be viable, the activity of kanamycin resistance protein (Kan^R) should be dependent on FucT2 solubility. Kan^R was fused to the C-terminus of mutant libraries of FucT2, which were generated using a combination of error-prone PCR and DNA shuffling. Notably, one round of the directed evolution process, which consisted of mutant library generation and selection based on kanamycin resistance, resulted in a significant increase in the expression level of soluble FucT2. As a result, a batch fermentation with the ΔL M15 pBCGW strain, expressing the FucT2 mutant (F#1-5) isolated from the first round of the directed evolution process, resulted in the production of 0.31 g/l 2'-FL with a yield of 0.22 g 2'-FL/g lactose, showing 1.72- and 1.51-fold increase in the titer and yield, respectively, compared to those of the control strain. The simple and powerful method developed in this study could be applied to enhance the solubility of other unstable enzymes.

• 전력전자학회논문지
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• 제16권5호
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• pp.511-520
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• 2011

현재 스마트 그리드 산업으로 인하여 신재생에너지의 활용이 중시되고 있다. 이러한 시점에서 신재생에너지원을 더욱 효율적이고 안정적으로 사용하기 위해서 에너지 저장장치의 필요성이 부각되고 있다. 이러한 경우 신재생 에너지원을 계통에 사용할 수 있도록 만들어 주는 부스트 컨버터와 에너지 저장장치의 에너지 전달을 위한충 방전기를 사용하게 된다. 이 두 가지 전력변환장치의 사용을 위해, 상호 간섭에 의해 각각의 제어기 설계에 어떠한 영향을 미치는지 알아야 한다. 우선 각각의 제어기 설계를 위해 시뮬레이션을 통한 간접적인 결과를 얻어내야 하는데, 이 때 인덕터를 이상적으로 생각하고 시뮬레이션을 할 수도 있고, 실제 하드웨어와 같이 인덕터에 기생하는 저항을 포함하여 시뮬레이션 할 수도 있다. 본 논문에서는 부스트 컨버터의 인덕터 기생저항이 부스트 컨버터와 충 방전기의 제어기 설계시 어떠한 영향을 미치는 지에 대해 비교해 봄으로써 시뮬레이션시 인덕터 기생저항 성분을 반드시 고려하여 설계해야 한다는 것을 주파수 영역에서의 MATLAB 시뮬레이션과 시간영역에서의 PSIM 시뮬레이션을 통해 살펴보았다.

• Structural Engineering and Mechanics
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• 제78권5호
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• pp.519-528
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• 2021

One of the most efficient designs of solar trackers for photovoltaic panels is the single-axis tracker, which holds the panels along a torque tube that is driven by a motor at the central section. These trackers have evolved to become extremely slender structures due to mechanical optimization against static load and the need of cost reduction in a very competitive market. Owing to the corresponding decrease in mechanical resistance, some of these trackers have suffered aeroelastic instability even at moderate wind speeds, leading to catastrophic failures. In the present work, an analytical and experimental approach has been developed to study that phenomenon. The analytical study has led to identify the dimensionless parameters that govern the motion of the panel-tracker structure. Also, systematic wind tunnel experiments have been carried out on a 3D aeroelastic scale model. The tests have been successful in reproducing the aeroelastic phenomena arising in real-scale cases and have allowed the identification and a close characterization of the phenomenon. The main results have been the determination of the critical velocity for torsional galloping as a function of tilt angle and a calculation methodology for the optimal sizing of solar tracker shafts.

• Steel and Composite Structures
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• 제10권5호
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• pp.385-414
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• 2010

In current European Standard EN 1993, the moment-rotation characteristics of beam-to-column joints made from steel with a yield stress > 460MPa are obtained from elastic design procedures. The strength of the joint basic components, such as the column web subject to local transverse compression, is thus limited to the yield resistance rather than the plastic resistance. With the recent developments of higher strength steel grades, the need for these restrictions should be revisited. However, as the strength of the steel is increased, the buckling characteristics become more significant and thus instability phenomena may govern the design. This paper summarizes a comprehensive set of finite element parametric studies pertaining to the strength behaviour of high-strength steel unstiffened I-columns in transverse compression. The paper outlines the implementation and validation of a three-dimensional finite element model and presents the relevant numerical test results. The finite element predictions are evaluated against the strength values anticipated by the EN 1993 for conventional steel columns and recommendations are made for revising the specifications.

• 한국전기전자재료학회논문지
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• 제29권3호
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• pp.135-140
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• 2016

In this study, we developed the solution-processed PMMA-$HfO_x$ hybrid ReRAM devices to overcome the respective drawbacks of organic and inorganic materials. The performances of PMMA-$HfO_x$ hybrid ReRAM were compared to those of PMMA- and $HfO_x$-based ReRAMs. Bipolar resistive switching behavior was observed from these ReRAMs. The PMMA-$HfO_x$ hybrid ReRAMs showed a larger operation voltage margin and memory window than PMMA-based and $HfO_x$-based ReRAMs. The reliability and electrical instability of ReRAMs were remarkably improved by blending the $HfO_x$ into PMMA. An Ohmic conduction path was commonly generated in the LRS (low resistance state). In HRS (high resistance state), the PMMA-based ReRAM showed SCLC (space charge limited conduction). the PMMA-$HfO_x$ hybrid ReRAM and $HfO_x$-based ReRAM revealed the Pool-Frenkel conduction. As a result of flexibility test, serious defects were generated in $HfO_x$ film deposited on PI (polyimide) substrate. On the other hand, the PMMA and PMMA-$HfO_x$ films showed an excellent flexibility without defect generation.

• 한국해양공학회지
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• 제15권3호
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• pp.75-80
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• 2001

The elastic plastic fracture toughness of this material is evaluated by the an unloading compliance method according to the ASTM E813-97 and E1152-97 method on the smooth and side groove 1CT specimens. The effect of smooth and side groove is studied on the material tearing modulus and characterizes the crack tip field under the plane stress and strain. SG-365 steel is observed that J-R curve and Tmat value decrease as 0%, 20%, 30%, and 40%. The 40% side grooved specimen is very useful in estimation of the $J_IC$. Because it is much easier than the smooth specimen to the onset of the ductile tearing by the R curve method. Besides. it improves the accuracy of toughness values, decreases the scattering the them and tunneling and shear lip by the side groove. Applicability of tearing modulus($T_J$ proposed by paris et al as instability panameter for this material is investigated.

• 한국추진공학회지
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• 제11권5호
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• pp.23-30
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• 2007

PSD(pogo suppression device)는 액체 추진 로켓의 추진제 공급부를 모사한 배관 시스템에 대한 기존의 실험 연구를 통해 배관 시스템의 동특성을 변화시킬 수 있음을 확인할 수 있었다. 본 연구에서는, PSD에 대한 수학적 모델을 만들고, 각 변수에 대한 값을 결정하는 과정에서 이론적으로 구하기 힘든 변수를 파악하여, 이를 기존의 실험 결과로부터 구하고자 하였다. 그를 위해, 선형화된 전달함수를 만들어 전체 시스템에 대한 불안정성을 판단하는 방법에 대해, PSD를 유체 섭동에 대한 연속 방정식 등의 지배 방정식을 이용하여, 제작된 PSD의 실제 적용시 중요한 이너턴스, 컴플라이언스, 레지스턴스 등의 변수를 수식화하고, 실험 결과를 이용하여 이를 정량화 하는 방법을 제시하였다.