• Title/Summary/Keyword: insecticides.

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Assessment of Contact and Oral Toxicity of Four Neonicotinoid Insecticides to Bumblebees (Bombus terrestris) (네오니코티노이드계 4종 농약의 서양뒤영벌 급성 접촉 및 섭식 독성평가)

  • Kim, Areumnuri;Kim, Boseon;Chon, Kyongmi;Lee, Hwan;Park, Yeon-Ki;You, Are-Sun;Park, Hong-Hyun;Yun, Hyeong-Ju
    • Korean Journal of Environmental Agriculture
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    • v.39 no.2
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    • pp.106-113
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    • 2020
  • BACKGROUND: Bumblebees have been shown to be very effective pollinators for most greenhouse tomatoes. Neonicotinoid insecticides are one of the most widely used pesticides in tomato crops in Korea. METHODS AND RESULTS: This study was carried out to investigate the toxicity of four neonicotinoid insecticides (clothianidin, dinotefuran, imidacloprid and thiamethoxam) to bumblebees based on the OECD guidelines (No.246, 247). The 48 hr LD50 (㎍ a.i. /bumblebee) values in the acute contact toxicity tests were determined as follows: clothianidin, 0.467; dinotefuran, 3.741; imidacloprid, 3.967; and thiamethoxam, 0.747. The 48 hr LD50 values in the acute oral toxicity tests were determined as follows: clothianidin, 0.005; dinotefuran, 0.056; imidacloprid, 0.325; and thiamethoxam, 0.018. The acute contact and oral toxicity of the test insecticides to bumblebees from most to least toxic was clothianidin > thiamethoxam > dinotefuran > imidacloprid. CONCLUSION: This study provided the basic toxicological data of neonicotinoid insecticides for bumblebees. In the near future, acute toxicity and mixture toxicity of other pesticides to bumblebees could be determined using this method.

Cross-resistance and Inheritance of Resistance in Laboratory-selected Strains of the Brown Planthopper(Nilaparvata lugens Stal) (벼멸구의 저항성 유발, 교차저항성 및 저항성 유전에 관한 연구)

  • 박형만;최승윤
    • Korean journal of applied entomology
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    • v.30 no.1
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    • pp.1-9
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    • 1991
  • Cross-resistance and inheritance of resistance in laboratory-selected strains of the brown planthopper to various types of the insecticides were investigated. The fenobucarb-selected ($R_{f}$), carbofuran-selected($R_{c}$), and diazinon-selected($R_{d}$) strains were 50.3, 49.2 and 5.8 times less sensitive to the corresponding insecticides than th susceptible strain. both $R_{f}$ and $R_{c}$ strains were highly resistant to the other carbamate insecticides, and moderately resistant to cypermethrin and deltamethrin, but nearly not resistant to fenvalerate and the organophosphorus insecticides except malathion and phenthate. Moderate resistance to malathion and phenthoate in the $R_{f}$ and $R_{c}$ strains was obtained at the rate of 13.0-12.0 and 8.5-7.5 times, respectively. The $R_{d}$ strain showed low levels of resistance to the carbamate, organophosphorus and pyrethroid insecticides, but negatively correlated cross-resistance to fenvalerate. Resistance of the brown planthopper to all the test insecticides was inherited by partially dominant autosomal factor(s).

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Insecticidal response of 14 insecticides against Korean Bemisia tabaci local populations (14종 살충제의 담배가루이 성충에 대한 살충제 약제반응 조사)

  • In-Hong Jeong;Leesun Kim;So-Hee Kim;Jong Hyeok Lee;Kyungjae Andrew Yoon;Si Hyeock Lee
    • Korean Journal of Environmental Biology
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    • v.41 no.4
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    • pp.627-636
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    • 2023
  • Bemisia tabaci Gennadius, one of the most invasive insect pests, has spread quickly nationwide since it was introduced to South Korea in 2008. The use of insecticides is the main management strategy for this pest, but the control efficiency has been decreased due to insecticide resistance. We collected 12 local B. tabaci populations to investigate the regional differences in efficiency and observed the mortality from 14 commercial insecticides applied at recommended concentrations (RC) and dilutions (0.1 RC and 0.01 RC) using the leaf dipping bioassay. Except for etofenprox (46-64%), thiamethoxam (37-60%), pyriproxyfen (21-61%), and pyridaben (61-65%), the other insecticides showed excellent insecticidal efficacy of 70% to 100% at their RC. In particular, flupyradifurone, emamectin benzoate, and cyantraniliprole showed high insecticidal efficacy of over 90% in all of the tested populations. Some insecticides that rapidly decreased in activity (less than 30%) at diluted concentrations or showed high resistance levels in nearby regions were classified for cautious use due to the possibility or potential to develop resistance. The results provide selected insecticides for B. tabaci control by region and could contribute to reducing insecticide abuse and increasing insecticidal efficiency in farming fields.

Mutagenicity of pesticides in the Salminella/Microsome System (Salmonella/microsomal enzyme activation system에서의 농약의 돌연변이 유발성)

  • Byeon, U-Hyeon
    • Korean Journal of Microbiology
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    • v.14 no.3
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    • pp.128-128
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    • 1976
  • 19 pesticides including 12 insecticides, 2 herbicides and 5 fungicides have been tested for mutagenic activity in the Salmonella/microsome system. It was found that insecticides, DDVP, Trichlorfon, Sumithion, Naled, fungicide, TMTD and herbicide NIP induced base substitute mutation and herbicide MO frameshift mutation. Mutagenicity of Sumithion and NIP was appeared only after rat microsomal enzyme activation and that of TMTD was increased after the microsomal enzyme activation.

Mutagenicity of pesticides in the Salminella/Microsome System (Salmonella/microsomal enzyme activation system에서의 농약의 돌연변이 유발성)

  • 변우현;현형환;이세영
    • Korean Journal of Microbiology
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    • v.14 no.3
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    • pp.123-134
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    • 1976
  • 19 pesticides including 12 insecticides, 2 herbicides and 5 fungicides have been tested for mutagenic activity in the Salmonella/microsome system. It was found that insecticides, DDVP, Trichlorfon, Sumithion, Naled, fungicide, TMTD and herbicide NIP induced base substitute mutation and herbicide MO frameshift mutation. Mutagenicity of Sumithion and NIP was appeared only after rat microsomal enzyme activation and that of TMTD was increased after the microsomal enzyme activation.

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Alterations of Blood Cholinesterase Activity and Urinary Para-nitrophenol Excretion After Exposure to Organophosphorus Insecticides (유기인제 농약 폭로로 인한 혈중 Cholinesterase 활성치와 요중 P-nitrophenol의 배설량의 변동)

  • Lee, Byung-Kook;Chung, Kyou-Chull
    • Journal of Preventive Medicine and Public Health
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    • v.7 no.1
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    • pp.115-121
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    • 1974
  • In order to evaluate the health hazard due to exposure to organophosphorus insecticides, we measured the blood cholinesterase activity ana urinary para-nitrophenol among 56 exposed subjects. They are orchard workers, rice plant workers and smithion factory workers. The clinical symptoms were also checked by physicians. We also measured the blood cholinesterase activity and urinary para-nitrophenol excretion of 20 urban people and 15 rural people who had never been exposed to organophosphorus insecticides in order to compare them according to age, sex and geographical differences. And these results were also compared with those of exposed groups. The results obtained were as follows. 1. The normal plasma cholinesterase activity and cell cholinestrase activity were $0.861{\pm}0.148\;{\Delta}pH/hr$ and $0.822{\pm}0.154\;{\Delta}pH/hr$. And normal para-nitrophenol in urine was $1.21{\pm}0.52mg/liter$. 2. No significant difference was existed in blood cholinesterase activities and urinary para-nitrophenol excre tion according to sex, age and geographical difference. 3. The plasma cholinesterase activity and cell cholinesterase activity of orchard workers, rice plant workers and smithion factory workers were $0.682{\pm}0.189\;{\Delta}pH/hr,\;0.775{\pm}0.160\;{\Delta}pH/hr,\;0.754{\pm}0.123\;{\Delta}pH/hr,\;and\;0.691{\pm}0.082\;{\Delta}pH/hr,\;0.756{\pm}0.117\;{\Delta}pH/hr,\;0.739{\pm}0.117\;{\Delta}pH/hr$. And significant decreses in blood cholinesterase activities were existed among orchard workers and smithion factory workers compared with control group. 4, The urinary para-nitrophenol excretions of orchard workers, rice plant workers and smithion factory workers were $1.33{\pm}0.66mg/liter,\;1.19{\pm}0.88mg/liter\;and\;1.37{\pm}0.67mg/liter$ and there were no significant difference between exposed groups and control group. 5. The clinical symptoms complained during and after organophosphorus insecticides exposure were frequently ranked by headache (67.7%) and vertigo (64.5%) and muscular ataxia and weakness (51.6%).

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Toxicity of several insecticides to Dichromothrips smithi Zimmermann(Thysanoptera : Thripidae) (난총채벌레의 살충제 감수성)

  • Ahn, Ki-Su;Lee, Ki-Yeol;Kang, Hyu-Jung;Park, Sung-Kyu;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.6 no.4
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    • pp.244-249
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    • 2002
  • This study was carried out to investigate the toxicities of 22 insecticides to Dichromothrips smithi. Insecticidal activity was evaluated by testing systemic action and residual effect in laboratory. All insecticides used in this study did not affect on the egg of D. smithi, although organophosphates such as fenitrothion, fenthion, methidathion, phenthoate, and phenthoate+ethofenprox suppressed the egg hatchability completely. On D. smithi larva fenitrothion, fenthion, methidathion, phenthoate, ethofenprox, thiamethoxam, abamectin, chlorfenapyr, emamectin benzoate, fipronil, spinosad, and phenthoate+ethofenprox showed 100% insecticidal activity. On D. smithi adult fenitrothion, fenthion, methidathion, phenthoate, ethofenprox, abamectin, emamectin benzoate, fipronil, spinosad, and phenthoate+ethofenprox showed 100% insecticidal activity. Root-uptake systemic effects of phenthoate on the larva of D. smithi was 43.3%. Whereas, systemic effect of other insecticides was less than 20%. Insecticide with more than 80% residual effect for 7 days after treatment were fenitrothion, fenthion, methidathion, phenthoate, ethofenprox, emamectin benzoate, fipronil, spinosad, and phenthoate.

Selection of low toxic insecticides for phytoseiid predatory mites, Amblyseius cucumeris and Amblyseius fallacis (Amblyseius cucumeris 및 Amblyseius fallacis에 대한 저독성약제 선발)

  • Choi, Byeong-Ryeol;Hilton, S.A.;Broadbent, A.B.
    • The Korean Journal of Pesticide Science
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    • v.7 no.4
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    • pp.296-301
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    • 2003
  • This study was conducted to select low toxic insecticides against natural enemies, and to evaluate resistance stability and cross-resistance to resistance strain for the fulfillment of integrated pest management development. Toxicity of imidacloprid and spinosad to Amblyseius cucumeris was relatively low regardless of the adopting test methods. In addition, those to the Amblyseius fallacis was also low by slide dipping method. The slide dipping method was useful to eliminate repellency effect by mites to the tested insecticides. Mortality of A. fallacis to deltamethrin recorded in 1994 and 1999 was 21.6% and 7.4%, respectively. Meanwhile, the permethrin-resistanct strain of A. fallacis was maintained its resistance to deltamethrin. However, the cross-resistance to the newly introduced insecticides namely imidacloprid, fipronil, chlorfenapyr, abamectin, and spinosad. was relatively low.

Studies on the Cholinesterase Inhibition and Toxicity of Various Organophosphorus Insecticides to the Hibernating Rice Stem Borer Larvae, Chilo suppressalis WALKER (이화명충에 대한 유기인살충제의 Cholinesterase 저해작용 및 살충력에 관하여)

  • Chang Chang Hyo;Saito Tetso;Iyatomi Kisabu
    • Korean journal of applied entomology
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    • v.10 no.1
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    • pp.13-22
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    • 1971
  • This experiment was conducted to investigate the differences of the in vitro inhibitory effect of various organephobphorua insecticides on the chlinesterase from rice stem borer and those of the toxicity of them against the insect, with special references to the relationship between the cholinesterase inhibition and the toxicity. The results obtained were summarized as follows: Phosphate compounds shelved stronger inhibitory effect on the cholinesterase than thhiophosphate compounds, but was not stronger in toxicity than the latter. Diethoxy compounds were not always stronger than dimethoxy in cholinesterase inhibition and the toxicity of organophosphorus insecticides. The organophosphorus insecticides that inhibited strongly the cholinesterase were not always stronger in the toxicity.

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Residual Analysis of Insecticides (Lambda-cyhalothrin, Lufenuron, Thiamethoxam and Clothianidin) in Pomegranate Using GC-μECD or HPLC-UVD

  • Hem, Lina;Park, Jong-Hyouk;Shim, Jae-Han
    • Korean Journal of Environmental Agriculture
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    • v.29 no.3
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    • pp.257-265
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    • 2010
  • In this study, the residual levels of four insecticidal compounds (lambda-cyhalothrin, lufenuron, thiamethoxam, and clothianidin) were monitored in the pomegranate, in order to assess the risk to consumers posed by the presence of such residues. The insecticides were applied at the recommended dose rates onto pomegranate trees. The samples were then collected at harvesting time after several treatments (two, three, and four treatments). After sample preparation progressed through the clean-up procedure, lufenuron, thiamethoxam, and clothianidin residues were analyzed via a HPCL-UVD, and the lambda-cyhalothrin residue was analyzed via a GC-${\mu}ECD$. The versatility of this method was evidenced by its excellent linearity (>0.9998 to 1) at broad concentration ranges. The mean recoveries evaluated from the untreated sample spiked with two different fortification levels ranged from 72.45 to 113.90%, and the repeatability (as a relative standard deviation) resulted from triplicate recovery tests was in a range from 0.80 to 11.75%. The residues of all insecticides determined from treated pomegranate samples and their LOD levels (lunfenuron, 0.01; lambda-cyhalothrin, 0.005; thiamethoxam, 0.01; clothianidin, 0.02 mg/kg) were much lower than their MRLs (0.5 mg/kg).