• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2005.05a
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• pp.85-85
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• 2005

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2016.09a
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• pp.156-160
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• 2016

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.11a
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• pp.113-113
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• 2002

• Journal of Forest and Environmental Science
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• v.36 no.4
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• pp.326-327
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• 2020

• The Korean Journal of Mycology
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• v.45 no.4
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• pp.394-398
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• 2017

A survey to assess the diversity of wound-associated Ophiostomatales and Microascales, the so-called ophiostomatoid fungi, on Korean native trees, was undertaken in 2017. Wounds were artificially created, and a fungus resembling a species of Sporendocladia was consistently isolated from the exposed cambium and inner bark of artificially induced wounds on Quercus variabilis. Morphological examination and DNA sequence comparisons based on the internal transcribed spacer (ITS) and 5.8S regions of the rDNA confirmed the identity of the fungus as Sporendocladia bactrospora. This is the first report on S. bactrospora occurring on Q. variabilis in Korea.

• The Plant Pathology Journal
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• v.35 no.1
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

• The Plant Pathology Journal
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• v.35 no.3
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• pp.208-218
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• 2019

Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.

• Journal of Forest and Environmental Science
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• v.36 no.2
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• pp.173-174
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• 2020

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.68.1-68.1
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• 2005

• Korean journal of applied entomology
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• v.59 no.4
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• pp.409-410
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• 2020

This report describes the damage caused by gypsy moths (Lymantria dispar) and stick bugs (Ramulus mikado) to forests and host plants in 2020. Severe tree-crown defoliation was found in central parts of Korea, especially Gangwon (1,638 ha), Gyeonggi (1,134 ha), Chungbuk (726 ha) and Seoul (476 ha). Stick bug outbreaks occurred in hilly areas, such as Mt. Bongsan, located between Seoul and Goyang.