• Current Research on Agriculture and Life Sciences
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• v.24
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• pp.37-42
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• 2006

The purpose of this study was a biota research and analysis for Close-to-nature stream restoration planning of Shinchun. The summary of this study is as follows; 1) The vascular plants in research area recorded of 45 species and insect fauna recorded of 34 species of 8 orders. As a result of table of community classification, the communities were two group; Quercus variabilis community(I), Pinus densiflora-Quercus variabilis-Quercus dentata community(II). 2) As a result of analysis on correlation of tree species, the level of significance in positive correlation between Quercus dentata and Corylus heterophyll aindicated 1% and between Pinus densiflora and Lespedeza bicolor also indicated 1%. 3) As a result of DBH analysis, it is expected that Quercus variabilis and Quercus dentata will dominateover other species in competition and its succession continuously maintains from now on in community I. In community II, it is assumed that there is a high possibility of changing into community of Quercus such as Quercus mongolica, Quercus dentata, and Quercus variabilis. 4) As a result of analysis on insect fauna, insect fauna consists of 94% of whole species as 32 species, 23 families, 8 orders. And 7 species, 7 families 4 orders was found in highly urbanized area, the vicinity of Sang-Dong bridge. 5) As mentioned above, Based on A biota fundamental research, Close-to-nature stream restoration planning were full of suggestions: i) Designating ecosystem preservation area, ii) Making Close-to-nature stream revetments, iii) Making pool-and-riffle, vi) Making decks for observation and walks for nature experience, v) Creating wetland biotope. Through these methods, it is necessary to promote bio-diversity and lead people to the space for eco-learning.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.77-80
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• 2004

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.2
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• pp.183-197
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• 2007

Field trial was conducted at the Research Farm of the Katsina State Agricultural and Rural Development Authority during the farming season of 2004 with a view to evaluate/determine the efficacy of some selected plant-derived biopesticides against the insect pests of cowpea as well as their effect on yield. The variety of the cowpea used was IT86D-719 and the plant derived biopesticides evaluated during the trial were chilli pepper, garlic, ginger, neem, sweetsop and tobacco. The experimental field was ploughed, harrowed and thereafter ridged before the commencement of the 2004 planting season. A total of twenty one (21) experimental plots were demarcated and arranged into seven treatment plots. The size of each plot was $5m{\times}4m$ while interspaces between adjacent plot and blocks were 1m and 2m, respectively. Results of the experiments showed that all the plant-derived biopesticide treatments were significantly (p>0.05) better than control treatment. The order of effectiveness of the treatments was tobacco (80-90%), sweetsop (75-85%), garlic (70-80), neem (72-78%), chilli pepper (60-70%) and ginger (30-50%). Furthermore, yield result corresponded positively with the effectiveness of the treatments. Results of the present finding suggest the use of tobacco, sweetsop and garlic as promising biopesticides in the control of cowpea insect pests.

• Korean journal of applied entomology
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• v.31 no.2
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• pp.174-181
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• 1992

Early, mid, and late maturing soybean varieties were artificially infested with larvae of Delia platura at seeding, and then later development and productivity of soybeans were studied. In addition, in hope to utilize as possible tactics in the management of D. platura in soybeans, cultural factors such as contents of organic matter in soil, soil types, and the use of plastic mulching on the degree of damage to soybean plants by D. platura were examined. Primary leaf damage by the larva delayed the forthcoming vegetative growth but not the blooming which is initiated by the change in day length. Degree of delay in growth was more severe where leaf damage was induced by insect infestation than where artificially excised, apparently owing to simultaneous damage in stems and roots besides primary leaves. Productivity of soybeans also dropped significantly in plants with 25 % or more loss in the primary leaf area by the larva. Both transparent and black plastic mulchings shortened the period from seeding to emergence of soybean thus reducing the chance of possible attack by the larva. Soybean seedlings grown in clay loam or clay were attacked less by the larva than those in other types of soil. High organic matter content in the soil enhanced speed of emergence resulting in less larval damage to the seedling.

• BMB Reports
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• v.33 no.6
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• pp.483-492
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• 2000

The Herpes simplex virus type 1 (HSV-1) strain F glycoprotein H (gH) gene in the pHLB-4 plasmid was recombinated into a baculovirus expression vector (lacZ-HcNPV) to construct a recombinant virus GH-HcNPV expressing gH. The sequences of gH and its expression were analyzed. The gH gene was located in the 6.41 kb BglII fragment. The open reading frame (ORF) of the gH gene was 2,517 bp and codes 838 amino acid residues. Insect cells infected with this recombinant virus synthesized a high level of the matured and gX-gH fusion protein with approximately 112 kDa. The fusion gH protein was localized on the membrane of the insect cells as seen by using immunofluorescence assay and accumulated in the cultured media by the SDS-PAGE and immunoprecipitation assays. The amino acid sequence presents additional characteristics compatible with the structure of a viral glycoprotein: signal peptide, putative glycosylation sites and a long C-terminal transmembrane sequence. Antibodies raised in mice to this recombinant protein recognized viral gH and neutralized the infectivity of HSV-1 in vitro. These results demonstrate that it is possible to produce a mature protein by gene transfer in eukaryotic cells, and indicate the utility of the HcNPV-insect cell system for producing and characterizing eukaryotic proteins. Furthermore, the neutralizing antibodies would appear to protect mice against HSV; accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.

• Korean journal of applied entomology
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• v.60 no.2
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• pp.185-191
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• 2021

A Quarantine Pest Research Facility (QPRF) had been build in Animal and Plant Quarantine Agency and designed to ensure alien pests are safety contained inside the laboratory. Functions of QPRF including insect rearing, research, and physical containment facility were verified to check suitability as a research laboratory using the beet armyworm, Spodoptera exigua. As insect rearing and laboratory facility, S. exigua completed its development for 32.2 days at 25℃. Egg hatching, pupation, and adult eclosion showed 80, 86.6, and 90%. Two pesticides, fluxametamide and hydramethylnon showed high susceptibility against S. exigua larvae by spraying and dipping methods. As physical containment facility, male adults, which were artificially released from laboratory, did not capture in delta trap installed sex pheromone lure during winter season. Based on these results, QPRF will be used for study on alien insect species.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1863-1870
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• 2016

Antimicrobial peptides/proteins (AMPs) are present in all types of organisms, from microbes and plants to vertebrates and invertebrates such as insects. The grasshopper Oxya chinensis sinuosa is an insect species that is widely consumed around the world for its broad medicinal value. However, the lack of available genetic information for this species is an obstacle to understanding the full potential of its AMPs. Analysis of the O. chinensis sinuosa transcriptome and expression profile is essential for extending the available genetic information resources. In this study, we determined the whole-body transcriptome of O. chinensis sinuosa and analyzed the potential AMPs induced by bacterial immunization. A high-throughput RNA-Seq approach generated 94,348 contigs and 66,555 unigenes. Of these unigenes, 36,032 (54.14%) matched known proteins in the NCBI database in a BLAST search. Functional analysis demonstrated that 38,219 unigenes were clustered into 5,499 gene ontology terms. In addition, 26 cDNAs encoding novel AMPs were identified by an in silico approach using public databases. Our transcriptome dataset and AMP profile greatly improve our understanding of O. chinensis sinuosa genetics and provide a huge number of gene sequences for further study, including genes of known importance and genes of unknown function.

• Korean Journal of Environmental Agriculture
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• v.40 no.1
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• pp.1-12
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• 2021

BACKGROUND: G protein-coupled receptors (GPCRs) are widely distributed in various organisms. Insect GPCRs shown as in vertebrate GPCRs are membrane receptors that coordinate or involve in various physiological processes such as learning/memory, development, locomotion, circadian rhythm, reproduction, etc. This study aimed to identify GPCRs expressed in fat body and compare the expression pattern of GPCRs in different temperature conditions. METHODS AND RESULTS: To identify GPCRs genes and compare their expression in different temperature conditions, total RNAs of fat body in Plutella xylostella larva were extracted and the transcriptomes have been analyzed via next generation sequencing method. From the fat body transcriptomes, genes that belong to GPCR Family A, B, and F were identified such as opsin, gonadotropin-releasing hormone receptor, neuropeptide F (NPF) receptor, muthuselah (Mth), diuretic hormone receptor, frizzled, etc. Under low temperature, expressions of GPCRs such as C-C chemokine receptor (CCR), opsin, prolactin-releasing peptide receptor, substance K receptor, Mth-like receptor, diuretic hormone receptor, frizzled and stan were higher than those at 25℃. They are involved in immunity, feeding, movement, odorant recognition, diuresis, and development. In contrast to the control (25℃), at high temperature GPCRs including CCR, gonadotropin-releasing hormone receptor, moody, NPF receptor, neuropeptide B1 receptor, frizzled and stan revealed higher expression whose biological functions are related to immunity, blood-brain barrier formation, feeding, learning, and reproduction. CONCLUSION: Transcriptome of fat body can provide understanding the pools of GPCRs. Identifications of fat body GPCRs may contribute to develop new targets for the control of insect pests.

• The Korean Journal of Pesticide Science
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• v.2 no.3
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• pp.137-146
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• 1998

Laboratory tests were carried out to evaluate the effect of several insecticides with insect growth regulator (IGR) properties on the larval development of housefly, Musca domestica, which was collected at a large pigpen in Hamyang, Gyeongnam, Korea in 1997. Commercial formulations of the chemicals were diluted with tap water into a range of concentrations, and mixed with larval media. In addition to the IGRs, imidacloprid 5% WP was tested, too. The IGRs treated at the 2nd instar stage induced higher larval mortalities than percentages of malformed pupae. The result were, however, opposite when the IGRs were treated at the 3rd instar stage. Overall mortality resulting from larval death and malformed pupae was dependent on concentration. Diflubenzuron, teflubenzuron, triflumuron, flufenoxuron, tebufenozide, and imidacloprid, treated to the 2nd instar larvae, showed mortality over 95 % at concentrations of 5 ppm, 3 ppm, 30 ppm, 5 ppm, over 1000 ppm, 1000 ppm, respectively. Higher concentrations were needed to get the same level. of mortality in the 3rd instar larvae as that in the 2nd larvae. Overall mortality over 95% at the 3rd instar could be get at concentrations of 100 ppm, 10 ppm, 300 ppm, 10 ppm of diflubenzuron, teflubenzuron, triflumuron, flufenoxuron, respectively. Tebufenozide (1,000 ppm) and imidacloprid (300 ppm) were less effective than the other chemicals, showing only 36.7% and 86.7% mortalities, respectively. The chemicals also affected pupal weight at high concentrations. Decrease of pupal weight was distinct at high concentrations of teflubenzuron, flufenoxuron, tebufenozide, imidacloprid. Diflubenzuron and triflumuron were less effective. From these results it could be concluded that the IGR insecticides can be used as control agents by interfering with moulting and pupation process of housefly, by reducing pupal weight which could be resulted in low fertility and less oviposition.

• Korean journal of applied entomology
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• v.36 no.2
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• pp.145-149
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• 1997

Occurrence pattern of major insect pests on 7 recommended potato cultivars in Korea was investigated from sowing to harvesting time at Kangneung and Daegwallyung experiment fields in 1996. Green peach aphid (Mym persicae Sulzer), potato aphid (Macrosiphum euphorbiae Thomas) and beet armyworm (Spodoptera enigua Hubner) were the major insect pests on potato leaves, and wireworm (Selatosomus puncticollis Mot.) was the major pest on tubers. There was a slight difference of average number of aphids per 50 leaves among cultivars; ranged from 22.7 on cv. Dejima to 46.3 on cv. Superior. Numbers of leaves damaged by beet armyworm larvae on cv. Shepody and cv. Jopung were 11.0 and 14.3, and these cultivars are thought to be resistant against the larvae infestation. However, degree of damage on cv. Dejima and cv. Namsuh was higher 10 times than cv. Shepody and cv. Jopung. In wireworm, the percentage of damaged tubers on cv. Irish Cobbler was lowest of 8.1%, and followed by cv. Superior, cv. Dejima, cv. Jopung, and cv. Atlantic. Whereas, those on cv. Namsuh and cv. Shepody were significantly high of 50.0% and 46.8%.