• Korean Journal of Agricultural Science
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• v.24 no.1
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• pp.21-28
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• 1997

For the investigation of occurrence of insect pests and their natural enemies, total number of 353 controlled agricultural farms were visited and surveyed. In the 73% of total controlled agricultural farms, 52 species insect pests were occurred. Among these pests, the western flower thrip (Frankliniella occidentalis), the two spotted spider mite (Tetranychus urticae), the greenhouse whitefly (Trialeurodes vaporariorum), the cotton aphid (Aphis gossypii) and the green peach aphid (Myzus persicae) were mainly occurred. On the other hand, small numbers of natural enemies were sustained in 40 controlled agricultural farms, which was 11% and 17% of visited farms and pest occurred farms, respectively. For the most part of collected natural enemies, there were included in Braconid, Coccinellid and Anthocorid, as 63, 20 and 8% of them, respectively.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.2
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• pp.155-162
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• 2002

Cytochrome P45O (CYP) gene has been known to play one of the most important roles in metabolizing the exogenous materials. In insect, CYP is particularly known to detoxify toxic materials by adding oxygen molecule to the hydrophobic region of the materials. Thus, CYP-dependent metabolism is associated with the adaptation of insect to host plant chemicals. This in turn is known to be one of the driving forces for CYP diversification. In the present study, we cloned seven gene fragments of CYP 4 (CYP4) family from the midgut of the beet armyworm, Spodoptera exigua, through RT.PCT, Sequence analysis of the product showed the gene fragment to contain an open reading frame of ~150 amino acids, consisted of ~450 bp. The cloned gene fragments contained typical, conserved regions found in CYP4 family. Pairwise comparison of the deduced amino acid sequences among seven clones ranged in divergence from 0% to 52.86% and resulted in five distinct clones. The other two clones were identical or differ by one amino acid respectively to the corresponding clone, although each differed by ten nucleotides. Analysis of correlation between GenBank-registered, full length CYP4 and the cloned fragments resulted in statistically significant relationship ($r^{2}$ = 0.96085; p < 0.001), suggesting utility of the partial sequences as such full-length sequences. Phylogenetic analysis of the clones with GenBank-registered insect and mammal CYP4 family sequences by parsimony and several distance methods subdivided the clones into two groups: tones belonging to CYP4S and the others to CYP4M families.

• International Journal of Industrial Entomology and Biomaterials
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• v.1 no.2
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• pp.95-102
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• 2000

Experiments related to the field of sericulture started in the years 1900, in Korea. The sericultural experimental station in Korea was first organized among agricultural fields in Korea, indicating that sericulture in Korea was regarded as an important field of agriculture. Sericulture has been devoted to a great deal for the improvement of Korean economy during the past 100 years even under the coarse social circumstances caused particularly by the Korean War, However, the traditional Korean sericulture, aimed to produce silk yarn, was weakened, because of several reasons such as diminishment in silk consumption, increased labor charge in Korea, and so on. After this difficulty time, the Korean sericulture was revolutionized by shifting into functional sericulture from 1995, and the Korean sericulture now plays an important role for the improvement of human health. Mulberry tree, silkworm, and silk have a boundless potential to be developed as resources. We expect the know-how obtained through silkworm research would expand to the other insect research too. Thus, an area of entomological industry is hoped to prosper owing to insect research as well as sericulture. Mulberry tree is known to possess many bio-active substances, so it can be utilized as a resource for substitute medicine and a raw material for the functional food. In addition, an invention of genetically engineered mulberry variety, which will produce more bioactive substances, is expected. Silkworm is one of the most extensively studied insect organisms on the genome so far, Thus, silkworm is expected to be an "insect bio-factory", enabling mass-production of useful proteins by transformation, in which useful foreign genes are assimilated into silkworm. Silk can be transformed into several phases, because it possesses useful functional groups, which are sensitive to chemical reaction. Also, because silk fibrin itself is protein, it has a superior applicability as tissue membrane. Due to this usefulness, many researchers are now working on the silk as food, cosmetic, medical resource, and bioengineering resource, and even an expanded application is expected using silk in the future. Until now, the researches on insects were largely focused on the prevention of the damage caused by pest, instead of a beneficial aspect. However, insects are thought to be the fourth natural resource in the world, possessing unlimited potential as world resources in the near future. Therefore, our entomological research effort should be focused on the subject with potential for industrialization. Such subject includes selecting the insect species useful for environmental evaluation, construction of environment-friendly agricultural ecosystem, pollen mediation, pet, and advanced bio-resources.

• Korean journal of applied entomology
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• v.22 no.2 s.55
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• pp.98-105
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• 1983

The rapid increase in cases of insect resistance to insecticides indicates that the contribution of present chemical control practices inevitably leads to exhaustion of available insecticide resources against key insect species. Now the problem of insecticide resistance exists worldwide among insects and mites affecting field crops and animals including human beings, ranging from minimal or absent in some developing countries, where use of insecticides has been low, to extremely severe in many developed countries. Since the occurrence of insect resistance to insecticides was firstly recognized in 1908, the increase in recent decades has been almost linear and now the number of species of insects and acarines in which resistant strains have evolved have been increased to a total of 432. Of these, $261(60\%)$ are agricultural importance and $171(40\%)$ of medical/veterinary importance. The phenomenon of insecticide resistance is asserting itself as the greatest challenge to effective chemical control of many important insect pests. Resistance of insects to insecticides has a history of nearly 80 years, but its greatest increase and its strongest impact have occurred during the last 40 years following the discovery and extensive use of synthetic organic insecticides and acaricides. The impact of resistance should be considered not only in terms of greater cost of pest control due to increased dosages and number of applications but also in terms of the ecological disruption of pest-beneficial species density relationships, the loss of investment in the development of the insecticides concerned, and socio-economic disruption in agricultural communities. Despite its grave economic consequences, the phenomenon of insecticide resistance has received surprisingly little attention in Korea. Since the study of insecticides started firstly in 1963, many entomologists have been concerned with this study. According to their results, some of the rice pests and some of the mites on orchard trees, for example, have developed worrisome level of resistance in several areas of this peninsula. With many arthropods, considerable advances in the developed countries have been made in the study of the biochemical and physiological mechanisms of resistance. Progress involves the biochemical characteristics of specific defense mechanisms, their genetics, interactions, and their quantitative and qualitative contribution to resistance. But their studies arc still inadequately known and relatively little have been contributed in terms of unique schemes of population management in achieving satisfactory pest control. It is apparent that there is no easy solution to resistance as a general phenomenon. For future challenging to effective control of insect pests which are resistant to the insecticides concerned, new insecticide groups with distinctly novel mode of action are urgently needed. It is clear, however, that a great understanding of the factors which govern the intensity of selection of field population for resistance could lead to far more permanently successive use of chemicals within the framework of integrated pest management than heretofore practiced.

• The Korean Journal of Pesticide Science
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• v.5 no.4
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• pp.11-19
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• 2001

This review discusses the effects and roles of insect hormones and insect growth regulators (IGRs) on vitellogenesis in adult insects. Insect vitellogenesis is regulated by hormones such as juvenile hormone (JH), ecdysteroids, and neurosecretory hormones (ovaryecdysteroidogenic hormone : OEH) released by neurosecretory cells, diet, and other elements(male specific protein of sperm fluid). In the fat bodies, the vitellogenins are synthesized by the stimulation of JH released by corpus allatum (CA) and ecdysteroids produced by follicle cells with the ovary in most insects. Furthermore, vitellogenins are released into the hemolymph, transported to the ovarioles by carrier protein, and incorporated into oocytes for the developing ovary. Of IGRs, juvenile hormone and its mimics such as methoprene and pyriproxifen appear to have pharmacological effects such as membrane lysis, destruction of salivary grand and midgut epithlial cells, fat body cells, and ovarian tissue, and also anti-juvenile hormone such as precocenes I and II appear to have specific cytotoxicity such as inhibition of corpus allatum and oocytes development. These results suggest that IGRs may be useful as agents for integrated pest management.

• Food Science and Industry
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• v.50 no.2
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• pp.2-11
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• 2017

Packaging is the last defensive barrier that protects food products from insect infestation during storage. However, though packaging films are hermetically sealed, insects can still be attracted by strong olfactory cues and penetrate through packaging materials, resulting in contamination. Insect contamination may cause consumers to be repulsed by contaminated food products. Especially, it is well known that stored-product insects cause critical problems in the cereal industry by inducing quantitative and qualitative damages to the grain products. The contaminations are caused by insects' metabolic byproducts and body parts, consequentially caused customer repulsion. Therefore, it is necessary to repel and control insects. However, management systems for storage insects in food industry have been inadequate for many years. Synthetic pesticides has been widely used, but pesticides may accumulate in foods, causing acute and chronic symptoms in consumers. For this reason, there is a growing need for the development of natural insecticides that can replace synthetic pesticides. Thus, various reports about anti-insect packaging materials and strategies to repel insects were introduced in this study. Furthermore, we suggested new strategies to develop an insect-repelling active packaging materials which could be applied in the food packaging industry.

• Food Science and Biotechnology
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• v.17 no.2
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• pp.219-227
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• 2008

Bacillus thuringiensis was isolated as a pathogen of the sotto disease of silkmoth larvae about a hundred years ago. Since then, this bacterium has attracted attentions of not only insect pathologists but also many other scientists who are interested in its strong and specific insecticidal activity. This has led to the recent worldwide development of B. thuringiensis-based microbial insecticides and insect-resistant transgenic plants, as well as a landmark discovery of par asp orin, a cancer cell-specific cytotoxin produced by B. thuringiensis. In this review, we describe examination of interaction between inclusion proteins of B. thuringiensis and brush border membrane of insects using a surface plasmon resonance-based biosensor, identification and characterization of parasporin-4, the latest parasporin produced by the B. thuringiensis A1470 strain, and an effective method for preparing the parasporin-4 from inclusion bodies expressed in the recombinant Escherichia coli cells.

• Proceedings of the KIEE Conference
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• 2004.11c
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• pp.156-158
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• 2004

Film printing companies have many problems during a printing process. Most of all, even an insect or dirt stick to the transparent film may cause severe errors until the end of printing job, which means big economic damage to the company. To prevent some insect-dots or dirt-dots, we have to Inspect the total area of film. However, it is very difficult to inspect the film in real-time due to the high-speed of printing that usually more than 150 m/min. A hardware based approach, for example DSP-based approach can be the one of solution candidates, but the total cost and the complexity increases the very high-level. In this paper, we suggest a software based approach, using MMX technology, to inspect the film in real-time. By Many real-plant experiments, we can see the suggest approach is applicable for the inspection of food packaging film in real-time.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.134-135
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• 2003

The insect cuticle undergoes drastic morphological alterations during postembryonic development and is an extracellular structure composed mainly of chitin and proteins that are synthesized and secreted by epidermal cells. Cuticle proteins, the major components of insect integument, are recently being focused as a model fur studying the mechanisms of gene regulation during molting and metamorphosis. (omitted)

• International Journal of Industrial Entomology and Biomaterials
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• v.34 no.1
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• pp.1-5
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• 2017

Bone morphogenetic protein 2 (BMP2) plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is involved also in cardiac cell differentiation and epithelial to mesenchymal transition. In this study, We expressed human BMP2 (hBMP2) recombinant protein using Baculovirus Expression Vector System (BEVS) in Sf9 insect cells. The hBMP2 cDNA was cloned into baculovirus transfer vector, pBacgus-4x-1 and recombinant baculovirus was screened out through X-gal and GUS-fusions assay. Western blot analysis shown that molecular weight of hBMP2 recombinant protein was about 44.71 kDa.