• Journal of Ecology and Environment
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• v.30 no.3
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• pp.209-223
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• 2007

Twenty-four wetlands located in Higashi-Hiroshima City in West Japan were selected for this study in order to investigate both the relationship between aquatic plant composition and environmental conditions; and the relationship between changing land use patterns in the catchments and the concentration of different forms of nitrogen in the wetlands. The dominant and subdominant species which comprised the principal vegetation were determined based on a vegetation census conducted in each wetland during the growing season from June to August, 2006. The seasonal variations of water quality factors (pH, electrical conductivity, turbidity, dissolved oxygen, total dissolved solid, and temperature) and different forms of nitrogen such as nitrite, nitrate, ammonium, total nitrogen, dissolved organic nitrogen and dissolved inorganic nitrogen concentrations were analyzed as important indicators of water quality for the surface water of the wetlands. The surveyed wetlands were classified into three types (non-disturbed wetlands, moderately-disturbed wetlands and highly-disturbed wetlands), based on the degree of human disturbance to their catchment areas. An analysis of variance indicated that there was a significant difference among the wetland groups in the annual mean values of electrical conductivity, total dissolved solids, total nitrogen, nitrite, dissolved inorganic nitrogen and dissolved organic nitrogen. Classification of the wetlands into three groups has revealed a pattern of changes in the composition of plant species in the wetlands and a pattern of changes in nitrogen concentrations. A majority of the non-disturbed wetlands were characterized by Brasenia schrebi and Trapa bispinosa as dominant; with Potamogeton fryeri and Iris pesudacorus as sub-dominant species. For most of the moderately-disturbed wetlands, Brasenia schrebi were shown to be a dominant species; Elocheriss kuriguwai and Phragmites australis were observed as sub-dominant species. For a majority of the highly-disturbed wetlands, Typha latifolia and T. angustifolia were observed as dominant species, and Nymphea tetragona as the sub-dominant species in the study area. An analysis of land use and water quality factors indicated that forest area played a considerable role in reducing the concentration of nutrients, and can act as a sink for surface/subsurface nutrient inputs flowing into wetland water, anchor the soil, and lower erosion rates into wetlands.

• Korean Journal of Agricultural Science
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• v.43 no.3
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• pp.424-431
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• 2016

The nutrients in livestock manure produced during fermentation processes in public livestock recycling centers are used as fertilizers. However, the large amounts of swine manure produced in intensive livestock farms can be a nonpoint source of pollution. In this experiment, we investigated the chemical properties, inorganic components, and heavy metal contents in 101 samples of liquid swine manure collected from 28 public livestock recycling centers throughout the nation. Results showed that the average pH of the samples was alkaline (pH range 5.18 to 9.54), and their maximum EC was $53.2dS\;m^{-1}$. The amounts of total nitrogen and total phosphorus were in the range of 1000 - 2000 and $200-800mg\;L^{-1}$ while potassium, which constituted 47% of the total inorganic ions recovered from the liquid swine manure, amounted to $1500mg\;L^{-1}$. The most distinctive heavy metals recovered from the liquid swine manure were copper and zinc although the amounts of both heavy metals were much lesser than those of the standards as livestock liquid fertilizer set by the Rural Development Administration. On the other hand, the amount of nitrogen decreased rapidly with an increasing fermentation period from immature to mature, assumed to be lost as volatile compounds, such as ammonia, which are the major odor components during the fermentation process.

• Animal Bioscience
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• v.35 no.5
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• pp.721-729
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• 2022

Objective: An experiment was conducted to determine the effects of supplementing graded concentrations of inorganic sulphur (S) without and with folic acid (FA) in maize-soybean meal diets on performance, slaughter and anti-oxidant variables, immune responses and serum protein fractions in broiler chicken. Methods: Inorganic S was supplemented at 0.05%, 0.10%, 0.15%, and 0.20% alone or in combination with FA (4 mg/kg) in basal diet (BD) containing no supplemental methionine (Met) and FA. A control group was fed with the recommended concentration of Met. Each diet was offered to 10 pens of 5 male broiler chicks (Cobb 400) and fed ad libitum from day 1 to 42. Results: The broilers fed the BD had lower body weight gain (BWG), feed efficiency (FE), higher lipid peroxidation (LP), lower activity of glutathione peroxidase (GSHPx), lower lymphocyte proliferation ratio (LPR), and reduced concentrations of total protein, albumin, and globulin in serum. Supplementation of FA and S to the BD improved the BWG (all concentrations of S) and FE (0.20% S) similar to the control group. Similarly, the combination of S and FA significantly improved the concentrations of total protein, albumin, and globulin in serum, reduced the LP and increased the activity of GSHPx and LPR. However, responses in the above parameters were related to the concentration of S in the diet. The slaughter variables and antibody titres against the Newcastle disease were not affected with the treatments. Conclusion: Based on the results, it is concluded that the combination of S (0.2%) and FA (4 mg/kg) improved the BWG and FE, similarly supplementation of these nutrients improved the concentration of protein fractions and reduced the stress (reduced LP and improved GSHPx) variables in serum and improved the cell mediated immune response (LPR) in broilers fed sub-optimal concentrations of Met in diet.

• Journal of Soil and Groundwater Environment
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• v.27 no.6
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• pp.1-10
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• 2022

This study assessed the feasibility of iron oxide nanoparticles impregnated with biochar (INPBC), derived from woody biomass, as a stabilizing agent for the stabilization of farmland soil in the vicinity of an abandoned mine through pot experiments with 28 days of lettuce growth. The lettuce grown in the INPBC amended soils increased by more than 100% and the concentrations of inorganic elements (Cu, Ni, Zn) decreased by more than 40%. As, Cd and Pb were not transferred properly from the soils to the lettuce biomass. The bioavailability of arsenic and heavy metals in the INPBC amended soils were decreased by 26%~50%. It seems that the major mechanisms of stabilization were arsenic adsorption on iron oxides, heavy metal precipitation by soil pH increasing and heavy metal adsorption on organic matter. These results revealed that the lower bioavailability of the inorganic pollutants in the soils stabilized using INPBC induced lower transfer to the lettuce. Thus, INPBC could be used as an amendment material for the stabilization of farmland soils contaminated by arsenic and heavy metals. However, a pre-review of the chemical properties of the amended soil must be performed prior to applying INPBC in farmland soil because the concentration of the nutrients in the soil such as available phosphates and exchangeable cations (Ca, Mg, K) could be decreased due to adsorption on the surface of the iron oxides and organic matter.

• Journal of the Korean Society for Marine Environment & Energy
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• v.17 no.4
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• pp.324-332
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• 2014

In order to reveal the long-term variations of water quality in Jinhae Bay, water qualities had been monitored at 9 survey stations of Jinhae Bay during 2000~2012. The surface and bottom waters concentrations of chemical oxygen demand (COD), dissolved inorganic nitrogen (DIN), dissolved inorganic phosphorus (DIP), and chlorophyll-a (Chl.-a) were higher at the survey stations of Masan Bay than the stations of other Bays. Especially, station 1 which is located at the inner area of Masan Bay had the highest values in the concentrations of COD, DIN, and Chl.-a because there were terrestrial pollutant sources near the station 1 and sea current had not well circulated in the inner area of Masan Bay. In factor analysis, the station 1 also had the highest factor values related to factors which increase organic matters and nutrients in surface and bottom waters of Masan Bay. However, the stations (st.5, st.6, st.7, st.8, and st.9) of other Bays had lower values of the factors. In time series analysis, the COD concentrations of the bottom waters at 8 stations except for station 1 distinctly decreased. However, the COD concentrations of the surface waters showed no distinct decrease trends at all stations. In the concentrations of nutrients (DIN and DIP) of both surface and bottom waters, there were tremendous decrease trends at all stations. Therefore, these distinct decrease trends of the COD in bottom waters and the nutrients in surface and bottom waters of Jinhae Bay could have been associated with water improvement actions such as TPLMS (total pollution load management system).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.455-461
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• 2002

limiting nutrients for phytoplankton were determined by dissored inorganic nitrogen/phosphorous (DIN/DIP) in situ and algal bioassay experiment in the Seomjin River estuary during a study period from March 1999 to October 2001. DIN/DIP ranged from 14.7 to 681.1 during the study period. DIN/DIP was over 16 at the upper and middle estuarine region where salinity was lower than 25 psu and chlorophyll a concentration was high, probably indicating P-limitation in this region while the ratio was less than 16 at the high saline (> 25 psu) region, reflecting the supply of DIP from Gwangyang Bay and thereby indicating N-limitation at the lower estuarine region. These results suggested that the spatial distribution of DIN/DIP in the study region was controlled by the high supply of phosphate from Gwangyang Bay, the low input from Seomjin River and the active uptake by phytoplankton within the estuarine system. The bioassay experiments using Skeletonema costatum, Thalaasiosira rotula and in situ phytoplankton assemblage displayed relatively higher growth of phytoplankton in the P-added culture media, indicating P-limitation. This result was well consistent with the spatial distribution of inorganic nutrients. S. costatum showed a rapid adaptation to the low salinity compared to other phytoplankton species. This phenomenon seemed to account for the strong (> $90\%$ in total cell number) S. costatum bloom in autumn in this estuary. Moreover, although phytoplankton growth rate was higher in the P-added culture media at the end of culture experiment of in situ phytoplankton, the fast growth in the trace metal-added media at the beginning of the experiment suggested a possibility of limitation by other micro-nutrients such as trace metal and vitamin etc.

• Journal of the Korean Society for Marine Environment & Energy
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• v.10 no.4
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• pp.235-243
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• 2007

In order to understand the water mass properties in the southern location of the East Sea in the Korean coasts, the vertical distributions (down to 2,000 m deep) of water temperature, salinity, and dissolved inorganic nutrients were determined in April of 2005. The water mass of the surveyed location showed distinct vertical layers; highly saline surface, surface mixing layer, and thermocline of low temperature and salinity. The water layer below 300 m was characterized by water temperature lower than $1^{\circ}C$ and salinity 34.06, showing a representative water mass of the East Sea. The inorganic nutrients rapidly increased from 200m in the northern and southern parts around Ulleung Basin. A marked environmental difference was found between two layers separated by thermocline. The upper layer of the thermocline was oligotrophic and the vertical distribution of nutrient was very stable. In the water layer between 100 and 200m the nutrients slightly increased but remained still stable. From southern coasts to northeastern Ulleung, the water mass properties were site specific; the thickness of the surface mixed layer and nutricline showed a trend diminishing toward the northern locations probably due to diminished influence of Tsushima water. Redfield ratio (N:P=16:1) based on the ratio of chemical composition in organism revealed that nitrogen value continuously decreased to less than 16 with the water depth down to loom from the thermocline. The value in the water layer deeper than 100 to 200 m, thereafter, showed an increasing trend (over 16). This result was further supported by the finding of lower chlrophyll a content in the layer.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.16 no.4
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• pp.196-205
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• 2011

To investigate the contribution of macroalgae to biogeochemical nutrients and carbon cycles, we measured the uptake rates of nutrients and $CO_2$ and characteristics of fluorescence of Saccharina japonica (Laminaria japonica Areschoug) using an incubation method in an acrylic chamber. From January to May 2011, S.japonica was sampled at Ilkwang, one of well-known macroalgae culture sites around Korea and ranged 46~288 cm long and 4.8~22.0 cm wide of whole thallus. The production rate of dissolved oxygen by S. japonica (n=25) was about $6.9{\pm}5.8{\mu}mol\;g^{-1}$ fresh weight(FW) $h^{-1}$. The uptake rate of total dissolved inorganic carbon ($TCO_2$), calculated by total alkalinity and pH, was $8.9{\pm}7.9{\mu}mol\;g^{-1}\;FW\;h^{-1}$. Mean nutrients uptake were $175.6{\pm}161.1\;nmol\;N\;g^{-1}\;FW\;h^{-1}$ and $12.7{\pm}10.1\;nmol\;P\;g^{-1}\;FW\;h^{-1}$. There were logarithmic relationships between thallus length and uptake rates of nutrients and $CO_2$, which suggested that younger specimens (<100-150 cm) were much more efficient at nutrients and $CO_2$ uptake than old specimens > 150 cm. There was a positive linear correlation ($r^2$=9.4) existed between the dissolved oxygen production rate and the $TCO_2$ uptake rate, suggesting that these two factors may serve as good indicators of S. japonica photosynthesis. There was also positive linear relationship between maximal quantum yield ($F_v/F_m$) and production/uptake rates of dissolved oxygen, $TCO_2$ and phosphate, suggested that $F_v/F_m$ could be used as a good indicator of photosynthetic ability and $TCO_2$ consumption of macroalgae. Maximum relative electron transport rate ($rETR_{max}$) of S. japonica increased as thallus grew and was high in distal part of thallus which may be resulted from the increase of photosynthetic cell density per area. The annual $TCO_2$ uptake by S. japonica in Gijang area was estimated about $1.0\sim1.7{\times}10^3C$ ton, which was about 0.02-0.03% of carbon dioxide emission in Busan City. Thus, more research should be focused on macroalgae-based biogeochemical cycles to evaluate the roles and contributions of macroalgae to the global carbon cycle.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1642-1649
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• 2003

An experiment was conducted to evaluate the effects of phytase supplementation on the growth performance, nutrients utilization and bone mineralization in broiler chickens. Day-old broiler chicks (n=480) were equally devided into eight treatment groups and fed maize or wheat based isocaloric, isonitrogenous and isocalcium diets having two non phytate phosphorus (NPP) concentrations (0.50% and 0.30%) and two phytase levels (0 and 500 phytase units/kg diet) in a 42 days growth trial. Maize based dietary treatments were MC (NPP 0.50%, MN (NPP 0.30%), MNP (MN+500 units of phytase) and MCP (MC+500 units of phytase), whereas wheat based experimental diets were WC (NPP 0.50%), WN (NPP 0.30%), WNP (WN+500 units of phytase) and WCP (WC+500 units of phytase). The NPP levels were maintained by dicalcium phosphate. Reduction in dietary NPP depressed live weight gain and feed intake and increased feed conversion ratio (FCR). Phytase supplementation to low NPP (0.30%) diets significantly (p<0.05) improved the growth performances of broilers. The supplementation to low NPP diets allowed complete, safe and economic replacement of dietary inorganic P (dicalcium phosphate) to reduce feed cost per kg live weight gain of broilers. Reduction in dietary NPP did not affect retention of nutrients except phosphorus (P) but had a significant (p<0.05) depression in tibia ash and minerals (Ca, P) concentration in serum and tibia ash. Phytase supplementation at low NPP level was effective (p<0.05) in improving the retention of dry matter, Ca and P and Ca and P concentration in serum and tibia ash. However, the supplementation was not effective at high level of NPP (0.50%). There were no significant (p>0.05) differences in carcass quality among dietary treatments. The response of phytase was greater in low NPP and maize based diets as compared with high NPP and wheat based diets, respectively. The results show that phytase supplementation to low NPP (0.30%) diets improved the growth performance, relative retention of nutrients (N, Ca and P) and minerals (Ca, P) status of blood and bone in broiler chickens, with a better efficacy in maize based diets.

• Journal of Nutrition and Health
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• v.6 no.3
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• pp.37-45
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• 1973

The main character of the Korean diet has been found to be low in protein both quantity and quality and high in carbohydrate. The purpose of this survey was to study the amount of salt intake related to the dietary pattern in Korea. The nutrition survey was conducted in a mountainous farming area located in Auhchun-ri, Gaebuk-myon, Changsoo-gun, Chunbuk Province, February 14-19 in 1973 (7 days). The precise weighing method was used in evaluating the kinds of foods and nutrients intake for 24 households during a three day period. The physical examinations were performed by a doctor on 120 persons and a detailed biochemical test on both blood and urine was made on 42 persons over 40 years old. The results obtained are summarized as follows: (1) Average nutrients intake of an adult per day: calorie intake was 2,446 Cal and its components-protein(61.1g) was 10 percent, fat(12.9g) was 5 percent and carbohydrate(521g) contributed 85 percent of the total calories. Other nutrients-calcium (443mg), thiamine(1.09mg), riboflavin (0.90mg), niacin (14.4mg) and vitamin C (63.2mg) were lower than the recommended daily allowance but vitamin A(2,083 I.U.), iron(11mg) and phosphorous(998mg) were slightly higher than that. (2) To evaluate the nutritional deficiences, clinical examinations were conducted. Angular stomatitis was present in 16.7 percent of those examiners. No edema was found. The rate of osteoarthritis, hepatomegaly diseases appeared in 20 percent of the total subjects and the symptoms appeared highest among those Iron 50 to 59 years old. (3) The following chemical components of blood serum were analyzed and found to be within the normal range: glucose, blood urea nitrogen, uric acid, total protein, albumin, globulin, bilirubin, total cholesterol, inorganic phosphate, alkaline phosphatase, sodium, potassium, chloride, calcium and lecithine dehydrase. One case of each of the following were found: hyperglycemia, hypocholesterolemia, renal problem, hypoproteinaemia and diabetes mellitus, and two persons were classified as showing hypoglycemia and hyponaturemia. (4) The sodium content in urine was 199.6 mEq/L, potassium content was 24.6 mEq/L. The sugar, pH and specific gravity in the urine was shown to be normal.