• Journal of The Korean Society of Grassland and Forage Science
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• 제34권3호
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• pp.163-168
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• 2014

This study was conducted to investigate the effects of bacterial inoculation (Lactobacillus plantarum) and cutting height on the chemical composition, fermentation characteristics and in vitro dry matter digestibility (IVDMD) in whole crop barley silage. Barley forage (Youngyang hybrid) was harvested at about 27% of dry matter (DM) level at two different cutting height (5 vs. 15 cm). And it was chopped to 5 cm length and treated with or without L. plantarum. Four replicates of each treatment were ensiled into 10 L mini silo (3 kg) for 100 days. After 100 days, bacterial inoculation decreased (p=0.001) DM content, while increased cutting height increased (p=0.002) DM in uninoculated silage. Crude protein (CP) concentration was decreased by increasing height in uninoculated silage (8.84 vs. 8.16) but increased in inoculated silage (8.19 vs. 8.99). Both neutral detergent fiber (NDF) (p<0.011) and acid detergent fiber (ADF) (p<0.004) were decreased by increasing cutting height of forage at harvest. The IVDMD and ammonia-N was increased (p=0.001) by increasing cutting height and inoculation, respectively. Lactic acid bacteria (LAB) was increased (p=0.002) in inoculated silage, but yeast count was decreased (p=0.026) in uninoculated silages. It is concluded that increased cutting height of forage at harvest could be useful to make a fibrous portion with increase of dry matter digestibility of silages.

• Journal of Mushroom
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• 제10권4호
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• pp.174-178
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• 2012

This study were carried out to analyze nutritional ingredients of pre-inoculation and post-harvest Flammulina velutipes media. pH, moisture content, total carbon(T-C), total nitrogen(T-N), crude protein, crude fiber, and crude fat content on the 8 raw materials using Flammulina velutipes bottle cultivation medium, and after sterilization(pre-inoculation) and post-harvest medium was analyzed. This result is expected to be utilized as the basis for recycling post-harvest media.

• Food Science and Biotechnology
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• 제18권1호
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• pp.253-256
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• 2009

The purpose of the study was to develop a new process to manufacture ginseng extract containing saponin aglycon of high concentration. The process to transform saponin glycosides to saponin aglycon was analyzed by high performance liquid chromatography (HPLC). GCK-1 (open cultured mixture for 1 day at $42^{\circ}C$) had the highest content of protopanaxadiol (0.662%). However, other mixtures (GCK-2, 3, 4, 5, and 6) had less than 0.152% in the content of protopanaxadiol. In case of fermentation by inoculation of Bacillus natto, BNG-5 (B. natto inoculated mixture for 5 days at $42^{\circ}C$) showed the highest content of protopanaxadiol (0.364%). Other mixtures (BNG-1, 2, 3, 4, and 6) also showed the high content of more than 0.2% in protopanaxadiol. B. natto inoculation or open culture fermentation with soybean transformed ginseng saponin glycosides into saponin aglycon.

• The Korean Journal of Mycology
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• 제6권1호
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• pp.21-27
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• 1978

The growing behavior of Candida albicans in various concentration of glucose and corticosteroid media was studied with the method of modified hanging slide culture. The strains of Candida albicans used in this study were obtained from vaginal swab from outpatients and were isolated from cultured colonies on Sabouraud's glucose agar media. To detect the budding rate of blastospore, the diluted suspension of Candida albicans in normal saline were inoculated into various concentration of glucose (Gl, G2, G3, G4), corticosteroid (S1, S2, S3, S4) and corticosteroid with 10% pepton broth (D1, D2, D3, D4) respectively and cultured at room temperature $(22{\sim}25^{\circ}C)$. The number of budding of blastospore were counted under the high power field of light microscope (400X) at specific time interval, e.g, 1, 2, 3, 6, 9, 12, 18, and 24 hours after inoculation. The results are as following: 1. The most effective budding rate was seen in G4 media (1.25% glucose) in 18 hours aft inoculation (89%). 2. The budding rate in Sabouraud's glucose broth with various concentration of dexamethasone added, was not significantly different from that of simple Sabouraud's glucose broth within 18 hours after inoculation, but there was statistically. significant difference in two budding rate at 24 hours observation. 3. The budding rate in 10% pepton broth media with various concentration of dexamethasone was almost same budding rate in control group, which is normal saline and 10% pepton broth, except on 2 and 24 hours results.

• Environmental Analysis Health and Toxicology
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• 제13권3_4호
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• pp.133-142
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• 1998

Effects of phosphorous (P) and methylglyoxal (MG) on the cell number, dry weight, chlorophyll content, photosynthetic and respiratory rate, phosphate uptake and protein content of green algae (Scenedesrnus obliquus) were studied. The algal cell number from the medium treated with 0.5-1.0 mM of MG at 1/2 P or 1/4 P concentration was significantly lower than those of algae treated :with full strength of phosphrous in medium. The inhibitory effect of MG on algal cell division was enhenced at low concentration of phosphorous in medium. At the beginning of logrithmic phase of algal growth, the mean dry weight of algae from the medium without MG-treatment in 1/2 P media was significantly higher than that of algae treated with MG. After logrithmic phase of growth cycle, the mean dry weight of algae from the medium with 1.0 mM of MG-treatment in 1/4 P media was significantly lower than that of algae treated with or without MG. At logrithmic phase of algal growth, there were significant differences in the chlorophyll content among all groups of tested algae with various concentrations of P and MG. At 15 days after inoculation, the mean chlorophyll content per algal cell from the media without MG-treatment in 1/2P was significantly higher than that of other cells from MG-treated media. The adverse effect of MG at concentration of 0.5-1.0mM in 1/2 and 1/4 P media on photosynthetic rate was observed. The mean photosynthetic rate of algal cell without P and MG treatment at 15 days after inoculation was significantly higher than that of MGtreated algae. After logarithmic phase, the algal cell treated with 0.5mM of MG with full strength of phosphorous showed significantly high respiratory rate than that of other cell groups. There were significant differences in mean phosphate uptake rate among all groups of Scenedesmus obliquus at logarithmic phase. At 12 days after inoculation, phosphate uptake rate per each algal cell from the basic media without MG and P treatment was rapidly reduced which shows early introduction to stationary phase.

• Environmental Engineering Research
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• 제24권4호
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• pp.670-679
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• 2019

Lignin complexity molecule makes its biodegradation difficult during lignocellulosic wastes composting. So, the improvement of its biodegradation has usually been considered as an objective. This study aimed to determine the impact of Trametes trogii inoculation on organic matter and particularly on lignin and cellulose during green wastes co-composting with olive mill waste water sludge and coffee grounds. Three types of heaps (H1, H2 and H3) were investigated during 180 d. H3 and H2 were inoculated at the beginning of the process (t₀) and 120 d later (t₁₂₀), respectively while H1 was the control. Results showed the absence of pH stabilization in H3 during the first month. Also, in this period we observed a faster degradation of some easily available organic matter in H3 than in the other heaps. After 120 d, a better cellulose decomposition (25.28%) was noticed in H3 than in H1 and H2 (16%). Inoculation during the second fermentation phase induced supplementary lignin degradation in H2 with a percentage of 35% against 23 and 26% for H1 and H3, respectively. For all the runs, a Fourier Transform Infrared analysis showed aliphatic groups' decrease, OH groups' increase and lignin structural modification.

• Journal of Bio-Environment Control
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• 제18권2호
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• pp.95-100
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• 2009

This study was conducted to develop an imaging processing system of inoculation processing of a lily callus. The image processing system was composed of a camera, a image processing board, and etc. And the illuminance always decided by setting up 55W/3 wavelength lamp respectively on all aspects and the side was maintained by the lighting part. The image characteristic was examined according to each frame of RGB,therefore the culture vessel was able to be separated with B frame. The required time was 2.2 seconds in one cycle from the image acquisition to obtaining the result. The recognition rate of the container was 100%, and the result of image processing showed that the recognition success rate of lily callus was 93%.

• Korean Journal of Organic Agriculture
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• 제23권3호
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• pp.497-507
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• 2015

It is necessary to evaluate the resistance to disease among genetic resources for development of disease resistant grapes. This study was conducted to screen the resistance to crown gall in muscadine and Florida hybrid bunch grapes by pathogen inoculation. In order to compare the responses to infection with different pathogen strains, muscadine and Florida hybrid grapes were inoculated with 3 strains of Agrobacterium vitis. Although there were different levels crown gall formation among grape cultivars, there little variation in response to inoculated strains. Among 29 muscadine cultivars tested by inoculation of A. vitis 'C4612', most of them were shown to be susceptible, and 'Gold Isle' and 'Africa Queen' were highly susceptible, and two cultivars, 'Welder' and 'Jumbo' were found to be resistant to crown gall disease. Among Florida hybrid grapes, 'Daytona', 'Stover', and 'Swanee' were susceptible and 'Blanc du Bois' was moderately susceptible to crown gall. Because muscadine grapes have been actively utilized as useful genetic resources for development of new grape varieties by intersub-genus cross, this result from the screening of resistance among muscadine grapes can provide valuable information in breeding programs of grape resistant to crown gall.

• Journal of Ginseng Research
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• 제43권1호
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• pp.77-85
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• 2019

Background: Glomus intraradices is a species of arbuscular mycorrhizal fungi that, as an obligate endomycorrhiza, can form mutually beneficial associations with plants. Panax ginseng is a popular traditional Chinese medicine; however, problems associated with ginseng planting, such as pesticide residues, reduce the ginseng quality. Methods: In this experiment, we studied the effect of inoculating G. intraradices on several physiological properties and microbial communities of ginseng. UV-Visible Spectrum method was used to detect physical properties. Denaturing gradient gel electrophoresis method was used to analyze microbial communities. Results: The results indicated that inoculation with G. intraradices can improve the colonization rate of lateral ginseng roots, increase the levels of monomeric and total ginsenosides, and improve root activity as well as polyphenol oxidase and catalase activities. We also studied the bacterial and fungal communities in ginseng rhizospheric soil. In our study, G. intraradices inoculation improved the abundance and Shannon diversity of bacteria, whereas fungi showed a reciprocal effect. Furthermore, we found that G. intraradices inoculation might increase some beneficial bacterial species and decreased pathogenic fungi in rhizospheric soil of ginseng. Conclusion: Our results showed that G. intraradices can benefit ginseng planting which may have some instructive and practical significance for planting ginseng in farmland.

• Journal of Chest Surgery
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• 제54권6호
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• pp.460-465
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• 2021

Background: Metastasis and recurrence of primary cancer are the main causes of cancer mortality. Disseminated tumor cells refer to cancer cells that cause metastasis from primary cancer to other organs. Several recent studies have suggested that circulating tumor cells (CTCs) are associated with the clinical stage, cancer recurrence, cancer metastasis, and prognosis. There are several methods of isolating CTCs from whole blood; in particular, using a membrane filtration system is advantageous due to its cost-effectiveness and availability in clinical settings. In this study, an animal model of lung cancer was established in nude mice using the human large cell lung cancer cell line H460. Methods: Six-week-old nude mice were used. The H460 lung cancer cell line was injected subcutaneously into the nude mice. Blood samples were obtained from the orbital area before cell line injection, 2 weeks after injection, and 2 weeks after tumor excision. Blood samples were filtered using a polycarbonate 12-well Transwell membrane (Corning Inc., Corning, NY, USA). An indirect immunofluorescence assay was performed with the epithelial cell adhesion molecule antibody. The number of stained cells was counted using fluorescence microscopy. Results: The average size of the tumor masses was 35.83 mm. The stained cells were counted before inoculation, 2 weeks after inoculation, and 2 weeks after tumor excision. Cancer cells generally increased after inoculation and decreased after tumor resection. Conclusion: The CTC detection method using the commercial polycarbonate 12-well Transwell (Corning Inc.) membrane is advantageous in terms of cost-effectiveness and convenience.