• Title/Summary/Keyword: inoculation

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Effect of Inoculum Density and Temperature on Clover Cyst Nematode, Heterodera trifolii (온도와 접종밀도가 클로버씨스트선충의 증식에 미치는 영향)

  • Jeong, Mungi;Lee, Dong Woon
    • Korean journal of applied entomology
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    • v.58 no.1
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    • pp.49-54
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    • 2019
  • Clover cyst nematode, Heterodera trifolii are one of the major pests that damage Chinese cabbage in high land cultivation area and have recently been distributed in Korea. This study was conducted to investigate the effect of temperature and inoculation density on the proliferation of clover cyst nematode. The clover cyst nematode was proliferated at $15-30^{\circ}C$ and the egg, female adult and cyst number was the highest at $20^{\circ}C$. As the inoculation density increased, the number of female cyst nematodes increased with the inoculation density and when 16 eggs were inoculated per gram of soil, the amount of growth, number of egg and cyst was the highest. Number of females and eggs were formed more in cabbage than in kale regardless of inoculation density. Chinese cabbage was a favorable host for clover cyst nematode growth than kale.

Gene Expression Analysis in Cucumber Leaves Primed by Root Colonization of Pseudomonas chlororaphis O6 upon Challenge-inoculation with Corynespora cassiicola.

  • Kim, M.;Kim, Y. C.;B. H. Cho
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.90.1-90
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    • 2003
  • Colonization of Pseudomonas chlororaphis O6, a nonpathogenic rhizobacterium, on the roots induced systemic resistance in cucumber plants against tai-get leaf spot, a foliar disease caused by Corynespora cassiicola. A cDNA library was constructed using mRNA extracted from the cucumber leaves 12 h after inoculation with C. cassiicola, which roots had been previously treated with O6. To identify the genes involved in the O6-mediated induced systemic resistance (ISR), we employed a subtractive hybridization method using mRNAs extracted from C cassiicola-inoculated cucumber leaves with and without previous O6 treatment on the plant roots. Differential screening of the cDNA library led to the isolation of 5 distinct genesencoding a GTP-binding protein, a putative senescence-associated protein, a galactinol synthase, a hypersensitive-induced reaction protein, and a putative aquaporin. Expressions of these genes are not induced by O6 colonization alone. Before challenge inoculation, no increase in the gene transcriptions could be detected in previously O6-treated and untreated plants but, upon subsequent inoculation with the pathogenic fungus, transcription levels in O6-treated plants rose significantly faster and stronger than in untreated plants. Therefore, the O6-mediated ISR may be associated with an enhanced capacity for the rapid and effective activation of cellular defense responses which becomes apparent only after challenge inoculation on the distal, untreated plant parts, as suggested by Conrath et al. (2002). This work was supported by a grant R11-2001-092-02006-0 from the Korea Science and Engineering Foundation through the Agricultural Plant Stress Research Center at Chonnam National University.

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Histological Detection of Phytoalexin Scoparone from Heat-Treated and UV-Illuminated Lemon Fruits After Inoculation with Penicillium digitatum

  • Kim, Jong-Jin;Yehoshua, Shimshon-Ben
    • The Plant Pathology Journal
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    • v.17 no.5
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    • pp.271-275
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    • 2001
  • Phytoalexin scoparone (6,7-dimethoxycoumarin) was induced in flavedo tissue of lemon fruit inoculated with Penicillium digitatum during heat treatment for 3 days at $36^{\circ}$. The compound was also induced in the flavedo tissue after UV illuminatiion. Induction of scoparone was deteected in the flavedo tissue by histological analysis. This fluorescent scoparone accumulated only on the 4-5 layers of cells adjacent to the inoculation site. Preinoculation with P. digitatum and subsequent heat-treatment induced resistance in the lemon fruit tissues after challenge-inoculation at the site of the first infection. the data obtained in the study suggest that lemon fruit acquired resistance against P. digitatum parallel with the scoparone production at the infection site.

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Occurrence of Narcissus Smoulder Caused by Botrytis narcissicola in Korea

  • Hong, Sung-Kee;Kim, Wan-Gyu;Cho, Weon-Dae;Kim, Hong-Gi
    • Mycobiology
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    • v.35 no.4
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    • pp.235-237
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    • 2007
  • Leaf blight and bulb rot symptoms were observed on narcissus plants grown in Yongin, Cheongwon and Namhae areas in Korea during disease survey from 1999 to 2002. A total of 15 isolates of Botrytis sp. were obtained from the infected plant parts of narcissus. All the isolates were identified as Botrytis narcissicola based on their morphological and cultural characteristics. Three isolates of B. narcissicola were tested for their pathogenicity to leaves and bulbs of narcissus by artificial inoculation. All the isolates induced leaf blight and bulb rot symptoms on the plants of narcissus by artificial inoculation. The symptoms induced by artificial inoculation were similar to those observed in the fields. This is the first report of narcissus smoulder caused by B. narcissicola in Korea.

Effect and Fading Behavior of Inoculant in the Cast Iron Melt (주철 용탕 중 접종제의 접종 효과와 감쇄 거동)

  • Lee, Hae-Seong;Kwon, Hae-Wook
    • Journal of Korea Foundry Society
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    • v.23 no.3
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    • pp.122-129
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    • 2003
  • The abilities and fading behaviors of 5 different inoculants were evaluated by the thermal analyses of the melts which were held at constant temperature after addition in induction furnace. The silicon content was preserved at the high holding temperature of 1,450${\sim}$1,500$^{\circ}C$, which reduced by oxidation at the low one. The inoculation temperature should be higher to obtain better and reproducible result. The inoculation performance of 1%Ba-Fe-Si was the best on the bases of effect and fading behavior.

Factors Affecting the Expression of Durable Resistance of Rice Cultivars to Blast caused by Pyricularia grisea Sacc 2. Components of the Resistance of Durably Resistant Cultivars for Rice Blast (벼 도열병에 대한 품종의 지속저항성 발현요인에 관한 연구 2. 도열병에 대한 지속저항성 품종의 저항성 구성요소)

  • 라동수;오정행;김장규
    • Korean Journal Plant Pathology
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    • v.13 no.2
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    • pp.85-89
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    • 1997
  • Etiological components of the rice cultivars possessing durable resistance to rice blast were evaluated. In the greenhouse test by artificial inoculation, number of leaf blast lesion in Dongjinbyeo was not increased by any race of the pathogen, while the lesion number on the resistant cultivar Palgongbyeo and susceptible cultivar Jinmibyeo was increased rapidly. Size of the lesion in Dongjinbyeo was smaller and progressed more slowly than the resistant cultivar Palgongbyeo until 11 days after inoculation. Latent period in Dongjinbyeo was intermediate. However, the period was significantly variable depending upon the races. Least amount of conidia was produced in Dongjinbyeo by race KJ-107 and the number of conidia was decreased rapidly from 11 days after inoculation.

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Survey on porcine eperythrozoonosis in Korea (국내(國內) 돼지의 에페리트로준병(病)에 대한 감염실태조사(感染實態調査))

  • Jeon, Yeong;Jang, Hwang
    • Korean Journal of Veterinary Research
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    • v.33 no.3
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    • pp.461-463
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    • 1993
  • A survey on procine eperythrozoonosis was conducted by the blood smear examinations and the animal inoculation tests with heparinized blood samples collected from slaughtered pig. The results obtained were as follows : 1. In the microscopic examination of the blood smears, 2(0.43%) of 455 slaughtered pigs in Seoul were infected with eperythrozoa, while none of 45 blood samples in Jeonbuk province was infected. The average infection rate of these areas was 0.4%. 2. In the animal inoculation tests, the eperythrozoa were detected in the splenectomized pigs 6 days after subcutaneous inoculation of the blood samples obtained from 100 pigs in Seoul, and the positive rate of slaughtered pigs was at least more than 1%.

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Degradation of Cholesterol by Bacillus subtilis SFF34 in Flatfish during Fermentation

  • Kim, Kwan-Pil;Rhee, In-Koo;Park, Heui-Dong
    • Journal of Microbiology
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    • v.41 no.4
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    • pp.284-288
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    • 2003
  • Bacillus subtilis SFF34 degrading cholesterol was applied to reduce residual cholesterol content in fermented flatfish. When the bacterial cells were inoculated as a start culture, a maximal level (1.7 U/g) of cholesterol oxidase was obtained after 10 days, which was two times higher than that (0.8 U/g) without inoculation. Residual cholesterol contents with and without inoculation of the cells were 0.5 mg/g and 0.8 mg/g after 12 days of fermentation, respectively. Cholesterol derivatives including cholesterol- 5${\alpha},\;6{\alpha}$-epoxide, 4-cholesten-3-one and 7${\beta}$-hydroxycholesterol were detected in raw flatfish as well as fermented flatfish. Campesterol and 25-hydroxycholesterol were detected only after fermentation. However, no significant differences in their contents were observed regardless of inoculation.

Comparison of Cultivation, Mushroom Yield, and Fruiting Body Characteristics of Lentinula edodes Strains according to the Inoculation Method

  • Jang, Yeongseon;Jeong, Yeun Sug;Ryoo, Rhim;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.49 no.4
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    • pp.525-530
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    • 2021
  • The cultivation in sawdust media, mushroom productivity, and fruiting body characteristics of Lentinula edodes strains NIFoS 2778 and NIFoS 3363 were compared according to the inoculation conditions. The cultivation period was 5% shorter when liquid spawn was used. Fruiting bodies were induced after 113 days of incubation on media inoculated with liquid spawn, and the cultivation period was 119 days on media inoculated with solid spawn. Mushroom productivity of NIFoS 2778 was the highest (661.4 g) when 36 mL of liquid spawn was used. For NIFoS 3363, mushroom production was higher under liquid inoculation conditions when the same amount of liquid and solid spawns were used. The mushroom characteristics of the two strains were not significantly different, except for gill width and stipe diameter.

Myositis unrelated to the inoculation site after COVID-19 vaccination: a case report

  • Jung Won Han;Jae Min Oh;Dae Hee Lee;Young Dae Jeon
    • Clinics in Shoulder and Elbow
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    • v.26 no.2
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    • pp.208-211
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    • 2023
  • We describe the case of a 49-year-old right hand-dominant woman with myositis of the biceps brachii muscle unrelated to the inoculation site following Pfizer-BioNTech COVID-19 vaccination on the deltoid muscle of the left shoulder. Coronavirus disease 2019 (COVID-19) pandemic has involved global spread, and different vaccines including inactivated, protein, vectored, and nucleic acid vaccines have been developed and administered. Common side effects of COVID-19 vaccines include general manifestations such as headache, fever, and fatigue, and various musculoskeletal symptoms. Here, we present a case of myositis occurring in the biceps brachii muscle unrelated to the inoculation site, which has not been reported previously, accompanied by a literature review.