• 한국발생생물학회지:발생과생식
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• 제14권3호
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• pp.155-162
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• 2010

The trophectoderm is one of the earliest cell types to differentiate in the forming placenta. It is an important for the initial implantation and placentation during pregnancy. Trophoblast stem cells (TBSCs) develop from the blastocyst and are maintained by signals emanating from the inner cell mass. However, several limitations including rarity and difficulty in isolation of trophoblast stem cells derived from blastocyst still exist. To establish a model for trophoblast differentiation, we isolated TBSCs from human term placenta ($\geq$38 weeks) and characterized. Cell cycle was analyzed by measuring DNA content by FACS analysis and phenotype of TBSCs was characterized by RT-PCR and FACS analysis. TBSCs have expressed various markers such as self-renewal markers (Nanog, Sox2), three germ layer markers (hNF68, alpha-cardiac actin, hAFP), trophoblast specific markers (CDX-2, CK7, HLA-G), and TERT gene. In FACS analysis, TBSCs isolated from term placenta showed that the majority of cells expressed CD13, CD44, CD90, CD95, CD105, HLA-ABC, cytokeratin 7, and HLA-G. Testing for CD31, CD34, CD45, CD71, vimentin and HLA-DR were negative. TBSCs were shown to decrease the growth rate when cultured in conditioned medium without FGF4/heparin as well as the morphology was changed to a characteristic giant cell with a large cytoplasm and nucleus. In invasion assay, TBSCs isolated from term placenta showed invasion activities in in vivo using nude mice and in vitro Matrigel system. Taken together, these results support that an isolation potential of TBSCs from term placenta as well as a good source for understanding of the infertility mechanism.

• Reproductive and Developmental Biology
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• 제33권3호
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• pp.133-137
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• 2009

Pluripotent embryonic stem (ES) cells isolated from inner cell mass (ICM) of blastocyst-stage embryos are capable of differentiating into various cell lineages and demonstrate germ-line transmission in experimentally produced chimeras. These cells have a great potential as tools for transgenic animal production, screening of newly-developed drugs, and cell therapy. Miniature pigs, selectively bred pigs for small size, offer several advantages over large breed pigs in biomedical research including human disease model and xenotransplantation. In the present study, factors affecting primary culture of somatic cell nuclear transfer blastocysts from miniature pigs for isolation of ES cells were investigated. Formation of primary colonies occurred only on STO cells in human ES medium. In contrast, no ICM outgrowth was observed on mouse embryonic fibroblasts (MEF) in porcine ES medium. Plating intact blastocysts and isolated ICM resulted in comparable attachment on feeder layer and primary colony formation. After subculture of ES-like colonies, two putative ES cell lines were isolated. Colonies of putative ES cells morphologically resembled murine ES cells. These cells were maintained in culture up to three passages, but lost by spontaneous differentiation. The present study demonstrates factors involved in the early stage of nuclear transfer ES cell isolation in miniature pigs. However, long-term maintenance and characterization of nuclear transfer ES cells in miniature pigs are remained to be done in further studies.

• 한국실내디자인학회논문집
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• 제15권3호
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• pp.118-125
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• 2006

A number of the retail and traditional market customer is decrease, whereas that of the supermarket in department-store customers is increase. This case suggests that customers have a preference for much more comfortable and pleasant shopping places And making a reasonable purchase in the supermarkets where we can find various goods and price zone, is now garden variety. It is a current course that once the manager ask an architect for multi-functional space design in department-store and then the architect compose a team and start to design. Of course, the team of planner thinking manage give the design team the basic material data such as commerce analysis and the use of each layer in the department store but, the design team solve the assignment by architectural form, functional space plan and the limited architecture law, After establishing general design for architecture, we can ask shopping-mall distribution, products display and interior design of the interior design team. so it is inevitable that the interior design team concerning M$\cdot$D can find lots of complementary factors with architecture design. The purpose of this study is analyzing the differences of architecture design, which has to accept the limited law and interior design concerning M$\cdot$D, satisfying the structure and the law in the future design for the department-store. Also the purpose of this thesis is suggestion the items architects and interior designers research into together to make the inner space ideally.

• Archives of Plastic Surgery
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• 제34권4호
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• pp.508-511
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• 2007

Purpose: Dermoid cysts are almost always caused by congenital events. The most widely accepted pathogenesis is that the cysts are dysembryogenetic lesions that arise from ectodermic elements entrapped during the midline fusion. We report a rare case of dermoid cyst, which occurred not congenitally but newly in the subcutaneous scar tissue secondary to trauma. Methods: A 26-year-old man had a deep submental laceration caused by a car accident and got a primary wound closure 16 months ago. There were 18 cm-long submental hypertrophic scar and newly developed palpable masses inside the subcutaneous layer at the center of the scar. Initial impression was an epidermal cyst or a thyroglossal duct cyst. Ultrasonographic finding showed two cystic masses inside the scars at the submental area, but impressed dermoid cysts. The cystic masses were completely removed with W-plasty and histological examination were followed. Results: The histological diagnosis was dermoid cysts which were mainly composed of keratotic squamous epithelium in their inner surface linings and numerous skin appendages such as sebaceous glands, sweat glands, and hair follicles in their cystic lumens histopathologically. During the follow-up period of 25 months, there was no recurrence of any subcutaneous mass in the site of scar. Conclusion: We report a very unusual case of dermoid cysts developed by an acquired cause, considering that the accidental inclusion of deep skin elements caused by a trauma can be a critical origin of dermoid cysts.

• Archives of Plastic Surgery
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• 제38권5호
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• pp.691-694
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• 2011

Purpose: With an increase in the population of immunocompromised patients, the incidence of maxillary sinus aspergillus infection has also escalated. Maxillary sinus aspergillosis is generally extended to the sinus antrum, base or thin orbital wall and ethmoid air cell region. We experienced a case of maxillary sinus aspergillosis which was extended directly to the soft tissue of the cheek. Methods: A 46-year-old man with acute myelogenous leukemia was consulted for the defect of the anterior wall of the maxillary sinus, and cheek. Radiologic and histologic findings were consistent with invasive maxillary sinus aspergillosis. The otolaryngology department performed debridement via endoscopic sinus surgery first. Coverage of the resulting defect in the anterior wall of the maxillary sinus and its inner layer was undergone by the plastic and reconstructive surgery department, using a pedicled superficial temporal fascia flap and a split thickness skin graft. The remaining skin defect of the cheek was covered with a local skin flap. Results: The patient went through an uneventful recovery. There was no recurrence during 6 months of follow-up. Conclusion: Maxillary sinus aspergillosis usually involves the orbit or the gingiva but in some cases it may directly invade soft tissues of the cheek. Such an atypical infection extending into the cheek may lead to a large soft tissue defect requiring coverage. Thus, any undiagnosed soft tissue defect involving the cheek or maxillofacial area, especially in immunocompromised patients, should be evaluated for aspergillosis. We present this rare case, with a review of the related literature.

• 한국해양학회지
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• 제29권2호
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• pp.95-106
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• 1994

김 등 (1993)에 의해 개발된 3차원 해수유동 수치모델을 사용하여 진해만의 조류 및 취송류를 해석하였다. 만내의 흐름은 주로 지형, 바람 및 하천유출에 의해 지배되 며, 만 입구의 수로보다 내만에서 바람 의 영향을 더 강하게 받는다. 계산된 조류는 현장관측과 잘 일치한다. 조류 및 조석잔차류의 수평 및 연직 유속은 만 입구의 수로 에서는 강하나, 진해만의 서부해역과 북부해역에서는 상대적으로 약하게 일어났다. 저 층과 표층 사이에 유속우 위상차가 일어나며, 위상차는 소조기보다 소조기보다 대조기 에 더 뚜렷하게 나타났다. 또한 만내에서 표층흐름은 바람 및 하천유출류에 의해 강하 게 지배되며, 이러한 현상은 대조기보다 소조기에 더 뚜렷하게 나타났다.

• 한국해양학회지:바다
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• 제8권2호
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• pp.164-176
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• 2003

하계 진동만에서 얻어진 영양염 DIN(dissolved inorganic nitrogen) 및 DIP(dissolved inorganic phosphate)의 분포는 이들 농도가 만안쪽에서 매우 높은 것이 특징이다. 또 표층(0∼1 m)과 저층(8 m∼bottom)은 만 안쪽이 고농도 이고 중층(1∼8 m)은 상대적으로 낮다. 이러한 영양염의 분포특성을 바람, 조류, 밀도의 수평경도력 및 잔차류를 고려한 생태계모델을 이용하여 조사하였다. 수치실험은 이들 조건들이 영양염에 각기 어떻게 영향을 미치는 가에 주목하여 실시되었다 조석잔차류를 고려한 경우는 전반적으로 관측값보다 저농도의 분포를 보였고, 바람, 조석잔차류 및 밀도의 수평경도력에 의한 효과를 포함한 경우는 보다 관측값에 근접한 결과를 얻었다 이러한 결과는 통계적 분석 방법에서도 잘 뒤받침된다. 본 연구결과는 하계 진동만에서 영양염의 분포변동에 조류효과 뿐만이 아니라 바람에 의한 취송류 및 밀도의 수평경도력에 의해 발생하는 밀도류의 효과가 매우 중요함을 시사한다.

• 대한두개안면성형외과학회지
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• 제12권1호
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• pp.48-52
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• 2011

Purpose: Many advances have been made in lower eyelid reconstruction surgical procedures after tumor ablative therapy. These include skin grafts, local flaps, free flaps, and skin expansion. When a full-thickness defect of the lower eyelid is reconstructed with many free flaps, ectropion and deformity of the medial and lateral canthal areas are common late complications caused by gravitational descent. The radial forearm free flap is widely used because of its lack of bulk, ease of dissection, malleability, and hairlessness. This report introduces a novel method for preventing ectropion using a composite radial forearm free flap reconstruction and palmaris longus suspension technique. Methods: A 70-year-old man had a malignant melanoma on his left lower eyelid. The patient was referred to our department after a biopsy confirmed the initial diagnosis. A full-thickness wide resection with a 25 mm free margin was performed, and a $5{\times}8cm$ radial forearm flap was elevated with a vascularised palmaris longus tendon. The palmaris longus tendon was fixed to the medial and lateral orbital rim perisoteum and the deep temporal fascia. The buccal mucosa was grafted to reconstruct the inner conjunctival layer. The pedicle vessels were anastomosed to the left superficial temporal artery and vein. Results: The postoperative clinical course was uneventful. The flap showed good texture and color match. No ectropion was noted 14 months after surgery and the tumor did not recur. The patient was quite satisfied with the final outcomes. Conclusion: Use of a radial forearm free flap and the palmaris longus tendon is an effective method for a full-thickness lower eyelid reconstruction.

• 한국입자에어로졸학회지
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• 제14권4호
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• pp.89-95
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• 2018

People's environmental concerns for fine particles in Korea lead to the strong necessity of improving the performance of environmental control systems. Wet electrostatic precipitators (ESPs) are considered as one of the alternatives to overcome the limit of previous dry ESPs, the re-entrainment of collected particles during rapping and back corona problem for high electrical resistivity dusts etc. In this study, a wire-cylindrical ESP with a thin water film has been developed. Particle collection characteristics were compared in the ESP with operations of water film on and off. Particle collection efficiencies at various applied voltages as well as voltage-current curves were almost the same in the ESP with and without a water film. Particle collection performance for PM1.0, PM2.5 and PM10 in the wet ESP with a water film was constantly maintained with operation time even in the high dust loading environment. This results indicate that a uniform water film in our wet ESP was successfully formed with a very thin layer without any dry spot and therefore could continuously clean the collected particles on the inner wall of the ESP without any performance degradation.

• Composites Research
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• 제32권4호
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• pp.177-184
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• 2019

이미 제작된 복합재료 제품을 분석하여 층별로 어떤 기계적 물성을 가지는지 알아낼 수 있다면, 기존 제품에 비해 더 좋은 성능을 내는 복합재료 개발을 수행할 수 있게 된다. 본 연구에서는 프리프레그를 적층하여 제작된 복합재료 구조물에 대해 역설계 기법을 적용하여 내부 프리프레그 층의 물성을 계산하고자 하였다. 단순히 인장시험으로 얻어지는 물리량을 이용한 경우와 인장시험 및 모드 해석을 통해 얻어지는 물리량을 이용한 경우를 비교한 결과 후자의 정확도가 더 높음을 알 수 있었다. 최종적으로 예측된 $E_1$의 최대 오차는 0.09%였고 예측된 $E_2$의 최대 오차는 7%였다.