• Archives of Pharmacal Research
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• 제14권2호
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• pp.188-192
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• 1991

Single non-lethal doses of chloroform $(CHCL_3)$ dichlorobromomethane $(CHCL_2Br)$, dibromochloromethane $(CHCIBr_2)$, or bromoform $(CHBr_3)$ were administered to male rats. Routes of exposure including single intraperitional (ip) and subcutaneous (sc) injection were used in order to permit comparison of severity of THM effects and renal toxicity was assessed at varied times following treatment. On an equimolar basis, sc administration of $CHBr_3$ (either 12 or 3 mmoles/kg) is more effective at increasing KW/BW than ip $CHCI_3$ treatment. Plasma urea nitrogen (BUN) following ip THM injections are markedly increased with all four THM at 24 hours post treatment. BUN response to $CHCL_2Br$ and $CHCIBr_3$-effected BUN levels have essentially returned to those of vehicle control. THM sc treatment results in a BUN response similar to that seen following ip treatment, with only the time course being different. With the exception of $CHCL_3$, sc and ip-treatments appear to be equally effective in evoking absolute BUN elevations. These results suggest that THM administration induce renal toxicity dependent upon the route or exposure.

• 한국식품영양과학회지
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• 제18권2호
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• pp.175-180
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• 1989

Vibrio 패혈증의 원인균인 V. vulnificus 에 대한 치사독소의 소재, 특성 및 독력을 밝히고저 해수에서 분리된 균(V. vulnificus S-1)과 환자에게 분리된 균(V. vulnificus M-1)을 새앙쥐 (institude cancer research mouse)에 복강 주사하여 실험하였다. 환자에서 분리된 균과 해수에서 분리된 균의 치사 독성은 차이가 없었으며 새앙쥐에 복강 주사하였을 때 $LD_{50}$은 $7.80{\times}10^6$ 이었다. V. vulnificus 의 조용혈소는 새앙쥐에 대한 치사독성이 없었으며 치사 독소는 균체 내에 존재하였으며 이 독소는 $80^{\circ}C$, 20분에 불활성화 되었다.

• 한국정보통신설비학회:학술대회논문집
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• 한국정보통신설비학회 2009년도 정보통신설비 학술대회
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• pp.355-358
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• 2009

센서 네트워크에 대한 연구가 활발히 이루어지면서 센서 네트워크 보안에 대한 문제점이 많이 야기되고 있다. 무선 센서 네트워크에서는 개방된 환경에서 제한적인 자원을 가지는 노드들로 구성되어 있다. 개방된 환경에 배치된 노드들은 공격자에게 쉽게 노출되어질 수 있다. 공격자는 노드를 물리적으로 포획하여 데이터 인증에 사용하는 인증키와 같은 보안 정보들을 획득할 수 있다. 공격자는 포획된 노드를 통하여 허위 보고서로 무선 센서 네트워크에 쉽게 삽입시킬 수 있다. 이는 허위 보고서로 인한 혼란 및 위조 정보의 전달과정에서 발생하는 에너지 고갈 등의 문제점을 유발시키게 된다. 이러한 허위 보고서를 조기에 탐지 및 폐기하기 위하여 동적 여과 프로토콜(DEF: Dynamic En-route Filtering scheme)이 제안되었다. DEF에서 인증키를 재배포 하는 주기는 보안 강도와 비용을 트레이드-오프 하는 관계에 놓여있으므로 매우 중요하다. 본 논문에서는 센서네트워크에서 동적 여과 프로토콜의 인증키 재배포 주기를 결정하는 기법을 제안한다. 배포된 노드들의 위상변화, BS까지 도달한 허위보고서 비율, 공격자에게 포획된 노드의 수 등을 고려하여 재배포 여부를 결정하고 재배포가 결정되면 각 클러스터 헤드들에게 재배포를 명령하게 된다.

• Toxicological Research
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• 제14권3호
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• pp.393-400
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• 1998

As a series of safety studies on DA-3585, a recombinant human erythropoietin, its local irritancy was examined in rabbits after the following treatments; application into the conjunctival sac of the eye(single), subcutaneous injection (single and -day repeated)and intravenous injection (7-day repeated.)In addition, perivascular irritation of DA-3585 was investigated in mice. In the result of ocular irritation test, 10,000IU/ml solution of DA-3585 could be considered as a non-irritating material. The local irritation of DA-3585 by a single and 7-day repeated subcutaneous injection was negligible and not so much different from that of saline. In the vascular irritancy test, macro-and microscopic observations revealed that local irritation of DA-3585 was comparable to that of saline when injected into retroauricular vein of rabbits for 7 consecutive days. Furthermore the perivascular administration of DA-3585 upto the concentration of 10,000 IU/ml did not induce any morphological abnormalities at injection sites. The results obtained from the present study suggest that the local irritancy of DA-3585 is not different from that of saline when injected through intravenous or subcutaneous route for clinical practice.

• Parasites, Hosts and Diseases
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• 제26권2호
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• pp.121-126
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• 1988

프라지관텔 주사제의 조충류 피료 효과를 평가하였다. 개, 고양이, 산양, 사슴, 닭, 쥐 및 마우스 등 총 93마리 동물의 장관내에 기성하는 조충류 dipylidium caninum, Taenia pisiformis, Spirometra sp., Taenia taeniaeformis, Moniekia expanse, Moniexia sp., Raillietina sP. 및 인ymenolepis nana에 체중 kg당 프라지콴텔 12 mg, 9 mg, 6 mg, 3 mg, 1.5 mg을 대퇴부에 근육주사한 후 치료효과를 판정하였다. H. nana에 감염된 마우스는 6mg/kg 투여 후 3시 간부터 6시 간 사이에 충체를 배출하였다. 다른 조충류도 약제투여 48시 간 이내에 모두 체외로 배출되었다. H. nana 및 M. expansa를 제외한 모든 충체는 체외 배출시 심하게 손상되어 있었으며, D.cnninum의 충란낭은 붕괴되어 충란이 분리되었다. 조충의 종류와 감염강도 등에 따른 숙주 체중 kg당 투여 용량의 차이는 관찰되지 않았으며 Spirometra sp.에 감염되었던 한 마리의 개를 제외하고는 프락지관텔 6 mg/kg 1회 용량으로 완치되어 97.9% (46/47)의 치유율을 보임으로써 우수한 치료 효과를 나타내었다.

• 한국발생생물학회지:발생과생식
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• 제14권1호
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• pp.35-41
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• 2010

Mammalian reproduction is regulated by a feedback circuit of the key reproductive hormones such as GnRH, gonadotropin and sex steroids on the hypothalamic-pituitary-gonadal axis. In particular, the onset of female puberty is triggered by gain of a pulsatile pattern and increment of GnRH secretion from hypothalamus. Previous studies including our own clearly demonstrated that genistein (GS), a phytoestrogenic isoflavone, altered the timing of puberty onset in female rats. However, the brain-specific actions of GS in female rats has not been explored yet. The present study was performed to examine the changes in the activities of GnRH neurons and their neural circuits by GS in female rats. Concerning the drug delivery route, intracerebroventricular (ICV) injection technique was employed to eliminate the unwanted actions on the extrabrain tissues which can be occurred if the testing drug is systemically administered. Adult female rats (PND 100, 210-230 g BW) were anaesthetized, treated with single dose of GS ($3.4{\mu}g$/animal), and sacrificed at 3 hrs post-injection. To determine the transcriptional changes of reproductive hormone-related genes in hypothalamus, total RNAs were extracted and applied to the semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). ICV infusion of GS significantly raised the transcriptional activities of enhanced at puberty1 (EAP-1, p<0.05), glutamic acid decarboxylase (GAD67, p<0.01) which are known to modulate GnRH secretion in the hypothalamus. However, GS infusion could not change the mRNA level of nitric oxide synthase 2 (NOS-2). GS administration significantly increased the mRNA levels of KiSS-1 (p<0.001), GPR54 (p<0.001), and GnRH (p<0.01) in the hypothalami, but decreased the mRNA levels of LH-$\beta$ (p<0.01) and FSH-$\beta$ (p<0.05) in the pituitaries. Taken together, the present study indicated that the acute exposure to GS could directly activate the hypothalamic GnRH modulating system, suggesting the GS's disrupting effects such as the early onset of puberty in immature female rats might be derived from premature activation of key reproduction related genes in hypothalamus-pituitary neuroendocrine circuit.

• The Korean Journal of Pain
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• 제1권2호
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• pp.154-163
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• 1988

In 1983, Sten H$\ddot{a}$kanson first reported the clinical safety and efficacy of retrogasserian glycerol injection as a treatment of typical trigeminal neuralgia in 96 of 100 patients during a follow-up period of 1~6 years. Since September 1987, we have injected sterile pure glycerol into the trigeminal cistern using an anterior percutaneous approach via the foramen ovale (H$\ddot{a}$rtel route) for treatment of tic douloureux in 12 patients who were suffering from attacks pain of following discontinuation of carbamazepine. The results were as follows; 1) Eight patients were completely free from pain attacks with a single dose of glycerol (0.4 ml). The remaining four patients needed a second dose (0.4 ml) several days later following the single dose. The degree of patient's subjective satisfaction by those injections was very good in 11 and fair in one. 2) During the follow-up period (1~13 months), persistent sensory deficit as determined by the pin prick test, appeared to be mild in 10 and moderate in one patient. There was no sensory deficit in one patient. further attacks of pain from those injections were still noted. 3) As a transient complication, there was headache in all patient, facial hematoma in 4, nausea and vomiting in two each, and vertigo and herpes simplex in one each. In conclusion, we confirmed that the above glycerol injections into the trigeminal cistern were clinically very effective as a treatment of tic douloureux even though the follow-up period was short.

• Animal cells and systems
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• 제4권1호
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• pp.71-76
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• 2000

The aim of this study was to investigate expression patterns of the prolactin (PRL) and c-fos genes by 17$\beta$-estradiol (17$\beta$-E) and stress in the mouse pituitary. In the pituitary, the levels of PRL mRNA were found high with some fluctuation at 30, 50, and 90 min whereas the levels of PRL mRNA were low at 120 min when ovariectomized female mice were injected with 17$\beta$-E or vehicle. PRL mRNA levels began to increase again at 4 h and remained high up to 24 h only in the 17$\beta$-E- treated mice. The overall changes in c-fos mRNA by 17$\beta$-E were very similar to those in PRL mRNA in the pituitary. Subsequent study revealed that these high initial levels of PRL and c-fos mRNAs were caused by stress during Injection, not by 17$\beta$-E, since vehicle injection alone into the ovariectomized mice could increase the levels of PRL and c-fos mRNAs. The stress-induced elevations of PRL and c-fos mRNAs were inhibited by bromocriptin, a dopamine agonist, suggesting that the dopaminergic system is involved in the action route of injection stress. In addition, the induced levels of c-fos mRNA by 17$\beta$-E and stress in the pituitary were very low compared with those in the uterus. The time course changes in c-fos mRNA level were different between the pituitary and uterus. Taken together, these data indicate that PRL and c-tos gene expression in the pituitary are regulated by 17$\beta$-E and stress in a parallel manner, supporting the notion that c-Fos plays a role in regulation of PRL gene expression.

• Tuberculosis and Respiratory Diseases
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• 제41권5호
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• pp.568-573
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• 1994

저자들은 급성 기관지 천식환자에서 치료제로 사용하는 hydrocortisone을 투여 후 유발된 기관지 수축이 발생한 예를 경험 하였기에 문헌고찰과 함께 보고하는 바이다.

• 한국화재소방학회논문지
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• 제14권2호
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• pp.14-20
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• 2000

건물화재시에 연기에 의한 피난자의 위험을 감소시키고 소방대의 구조활동을 원활히 하기 위하여 건물 외부의 공기를 이용, 건물내부의 일부를 가압하는 가압방연시스템이 이용되고 있다. 본 연구에서는 건물의 가압방연시스템을 설계하기 위하여 건물의 각 요소, 방, 로비, 계단실, 급기샤프트 등을 연결하는 유로를 구성하여 각 요소에서의 압력손실과 유량을 계산할 수 있는 컴퓨터 프로그램을 개발하였다. 본 프로그램을 이용하여 가압용 송풍기의 적절한 용량선정과 송풍기의 용량 최적화를 위한 방안을 강구하였다.