• Journal of Life Science
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• v.29 no.7
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• pp.785-791
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• 2019

In this study, we investigated the biological antioxidant and antibacterial activity of Chamaecyparis obtusa (C. obtuse) extracts by measuring DPPH radical scavenging and ABTS radical scavenging, and SOD-like activities. The DPPH and ABTS radical scavenging activities were increased in a dose-dependent manner, with maximum activities of 78% and 62% at an extract concentration of $50{\mu}l/ml$. The C. obtusa extracts also showed high SOD-like activity, with a maximum activity of 92.85% at a concentration of $50{\mu}l/ml$. The antibacterial activities of C. obtusa extracts were measured against six types of bacteria known to cause food poisoning and disease. Antibacterial activity was investigated against three gram-positive and three gram-negative bacteria using the paper disc agar diffusion method. The C. obtusa extracts showed antibacterial activities against B. cereus, E. coli, L. monocytogenes, S. aureus, S. typhi and V. parahaemolyticus, among which the activity against B. cereus was greatest. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of C. obtusa extracts were $30-40{\mu}l/ml$ for the 6 strains that showed an antimicrobial response by the paper disc agar diffusion method. These results suggest that C. obtusa extracts could serve as potential antibacterial agents to inhibit the growth of pathogens responsible for food poisoning and disease.