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http://dx.doi.org/10.5352/JLS.2019.29.7.785

Antibacterial and Antioxidant Activity of Chamaecyparis obtusa Extracts  

Kim, Bo Kyung (Department of Food & Nutrition, Silla University)
Kang, Jeong Hyeon (Department of Food & Nutrition, Silla University)
Oh, Geun Hye (Department of Food & Nutrition, Silla University)
Hwang, Ji-Young (Department of Food Science and Technology, Dong-eui University)
Jang, Seok Oui (Deok Hwa Food Co.)
Kim, Mihyang (Department of Food & Nutrition, Silla University)
Publication Information
Journal of Life Science / v.29, no.7, 2019 , pp. 785-791 More about this Journal
Abstract
In this study, we investigated the biological antioxidant and antibacterial activity of Chamaecyparis obtusa (C. obtuse) extracts by measuring DPPH radical scavenging and ABTS radical scavenging, and SOD-like activities. The DPPH and ABTS radical scavenging activities were increased in a dose-dependent manner, with maximum activities of 78% and 62% at an extract concentration of $50{\mu}l/ml$. The C. obtusa extracts also showed high SOD-like activity, with a maximum activity of 92.85% at a concentration of $50{\mu}l/ml$. The antibacterial activities of C. obtusa extracts were measured against six types of bacteria known to cause food poisoning and disease. Antibacterial activity was investigated against three gram-positive and three gram-negative bacteria using the paper disc agar diffusion method. The C. obtusa extracts showed antibacterial activities against B. cereus, E. coli, L. monocytogenes, S. aureus, S. typhi and V. parahaemolyticus, among which the activity against B. cereus was greatest. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of C. obtusa extracts were $30-40{\mu}l/ml$ for the 6 strains that showed an antimicrobial response by the paper disc agar diffusion method. These results suggest that C. obtusa extracts could serve as potential antibacterial agents to inhibit the growth of pathogens responsible for food poisoning and disease.
Keywords
Antioxidant; antibacterial activity; Chamaecyparis obtuse; inimum bactericidal concentration (MBC); Minimum inhibitory concentration (MIC);
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1 Kim, T. S., Kim, M. J., Kang, Y. M., Oh, G. N., Choi, S. Y., Oh, M. S., Yang, Y. S., Seo, J. M., Ryu, M. G., Kim, E, S., Ha, D. R. and Cho, B. S. 2014. Molecular characterization and toxin profile of bacillus cereus strains isolated from ready-to-eat foods. Kor. J. Food Sci. Technol. 46, 334-340.   DOI
2 Kim, Y. D., Kim, H. K. and Kim, K. J. 2003. Antimicrobial activity of solvent fraction from Cornus officianalis. Kor. J. Food Sci. 32, 829-832.   DOI
3 Kwon, T. D., Choi, S. W., Lee, S. J., Chung, K. W. and Lee, S. C. 2001. Effects of polyphenol or vitamin C ingestion on antioxidative activity during exercise in rats. Kor. J. Phys. Edu. 3, 891-899.
4 Lee, B. W. and Shin, D. H. 1991. Screening of natural antimicrobial plant extract on food spoilage microorganisms. Kor. J. Food Sci. Technol. 23, 200-204.
5 Lee, C. E., Jo, J. K., Kim, J. D., Lee, D. G., Kim, W. S. and Lee, S. H. 2017. Verification of antibacterial activity of herbal medicine extracts. J. Life Sci. 27, 611-616.   DOI
6 Lee, J, W., Yoon, J. H. and Park, J. W. 2015. Effect of grapefruit seed extract fir abtubacteruak activity on the coated packaging. Food Eng. Prog. 19, 104-110.   DOI
7 Lee, K. M., Jeong, G. T. and Park, D. H. 2004. Study of antimicrobial and DPPH radical scavenger activity of wood vinegar. KSBB J. 19, 381-384.
8 Lee, Y. C., Oh, S. W. and Hong, H. D. 2002. Antimicrobial characteristics of edible medicinal herbs extracts. Kor. J. Food Sci. Technol. 34, 700-709.
9 Kim, S. M., Kim, E. C., Choi, M. R., So, H. A., Shim, E. S., Kim, E. S., Park, S. C., Seong, C. N. and Chong, Y. C. 2008. Cytolethal distending toxin production, genotypes and atimicrobial susceptibility of Campylobacter jejuni isolates from diarrhea patients and chickens. J. Bacteriol. Virol. 38, 207-219.   DOI
10 Lee, Y. M., Bae, J. H., Kim, J. B., Kim, S. Y., Chung, M, N., Park, M. Y., Ko, J. S., Song, J. and Kim, J. H. 2012. Changes in the physiological activities of four sweet potato varieties by cooking condition. Kor. J. Nutr. 45, 12-19.   DOI
11 Lee, Y. S., Choi, J. B., Joo, E. Y. and Kim, N. W. 2007. Antioxidative activities and tyrosinase inhibition of water extracts from Ailanthus altissima. Kor. J. Food Nutr. 36, 1113-1119.   DOI
12 Lee, Y. S., Joo, E. Y. and Kim, N. W. 2005. Antioxidant activity of extract from the Lespedeza bicolor. Kor. J. Food Preserv. 12, 75-79.
13 Lehrer, R. I., Rosenmen, M., Harwig, S., Jackson, R. and Eisenhauer, P. 1991. Ultrasenstive assays for endogenous antimicrobial polypeptides. J. Immunol. Methods 137, 167-173.   DOI
14 Marklund, S. and Marklund, G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. FEBS J. 47, 469-474.
15 Moon, Y. G., Choi, K. S., Lee, K. J., Ki, K. Y. and Heo, M. S. 2006. Screening of antioxidative and antibacterial activity from hot water extracts of indigenous plants, Jeju-island. KSBB J. 21, 164-169.
16 Park, U. Y., Chang, D. S. and Cho, H. R. 1992. Screening of antimicrobial activity for medicinal herb extracts. Kor. J. Food Sci. 21, 91-96.
17 Park, C. S. and Cha, M. S. 2000. Comparison of antibacterial activies of green tea extracts and preservatives to the pathogenic bacteria. Kor. J. Food Nutr. 13, 36-44
18 Park, E. R., Lee, S. K., Hwang, H. S., Mun, C. S., Gwak, I. S., Kim, O. H. and Lee, K. H. 2008. Monitoring of natural preservative levels in food products. Kor. J. Food Nutr. 37, 1640-1646.   DOI
19 Park, H. K. and Kim, S. B. 2006. Antimicrobial activity of grapefruit seed extract. Kor. J. Food Sci. 19, 526-531.
20 Park, Y. J., Jung, S. M., Yoo, S. A., Kim, W. U., Cho, C. S., Park, B. J., Woo, J. M. and Yoon, C. H. 2015. Antinociceptive and anti-inflammatory effects of essential oil extracted from Chamaecyparis obtusa in mice. In Immunopharmacol. 29, 320-325.   DOI
21 Piddock, L. J. 1990. Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. J. Appl. Bacteriol. 68, 307-318.   DOI
22 Shi, J., Gong, J., Liu, J. E., Wu, X. and Zhang, Y. 2009. Antioxidant capacity of extract from edible flowers of Prunus mume in China and its active components. LWT-Food Sci. Technol. 42, 477-482.   DOI
23 Tokoro, A., Kobayashi, M., Tatewakio, N., Sucuki, K., Okawa, Y., Mikami, Y., Suzuki, S. and Suzuki, M. 1989. Protective effect of N-acetyl chitohexaose on Listeria monocytogenes infection in mice. Microbiol. Immunol. 33, 357-367.   DOI
24 Cano, A., Hernandez Ruiz, J., Garcia Canovas, F., Acosta, M. and Arnao, M. B. 1998. An end point method for estimation of the total antioxidant activity in plant material. Phytochem. Anal. 9, 196-202.   DOI
25 Wang, M., Li, J., Rangarajan, M., Shao, Y., LaVoie, E. J., Huang, T. C. and Ho, C. T. 1998. Antioxidative phenolic compounds from sage (Salvia officinalis). Agric. Food Chem. 46, 4869-4873.   DOI
26 An, S. A., Cho, Y. H., Ha, S. D., Park, K. H. and Yoon, K. R. 2003. Case study on risk assessment of domestic food preservatives. Food Sci. Indus. 36, 72-78.
27 Azuma, K., Nakayama, M., Koshika, M., Lppoushi, K., Yamaguchi, Y., Kohata, K., Yamaguchi, Y., Ito, H. and Higashio, H. 1999. Phenolic antioxidants from the leaves of Corchorus olitorius L. J. Agric. Food Chem. 47, 3963-3966.   DOI
28 Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181, 1199-1200.   DOI
29 Brewer, M. S., Sprouls, G. K. and Russan, C. 1994. Consumer attitudes toward food safety issues. J. Food Safety 14, 63-76.   DOI
30 Chen, Z., Bertin, R. and Froldi, G. 2013. EC50 estimation of antioxidant activity in DPPH assay using several statistical programs. Food Chem. 138, 414-420.   DOI
31 Cho, M. L., Lee, J. S., Lee, S., Son, Y. K., Bae, C. H., Yeo, J. H., Lee, H. S., Ma, J. G., Lee, O. H. and Kim, J. Y. 2015. Antioxidant activity of 11 species in Korean native forest plants. Kor. J. Food Nutr. 28, 1098-1106   DOI
32 Choi, J. S., Oh, J. I., Hwang, I. T., Kim, S. E., Chun, J. C., Lee, B. H., Kim, J. S., Kim, T. J. and Cho, K. Y. 2003. Application and high throughput screening of DPPH free radical scavenging activity by using 96-well plate. Kor. J. Pestic. Sci. 7, 92-99.
33 Han, K. I., Kim, M. R., Jo, B. K., Kim, M. J., Kang, M. J., Park, K. H., Koo, Y. E., Kim, B. S., Jung. E. G. and Han, M. D. 2015. Antimicrobial and antioxidative activities of the extracts from walnut (Juglans regia L.) green husk. J. Life Sci. 25, 433-440.   DOI
34 Choi, Y. M., Kim, M. H., Shin, J. J., Park, J. and Lee, J. S. 2003. The antioxidant activities of the some commercial teas. Kor. J. Food Nutr. 32, 723-727.   DOI
35 Chung, H. J. 2000. Antioxidative and antimicrobial activities of Opuntia ficus indica var. saboten. Kor. J. Food Nutr. 16, 160-166.
36 Gould, G. W. 1996. Industry perspectives on the use of natural antimicrobials and inhibitors for food applications. J. Food Prot. 59, 82-86.   DOI
37 Hong, N. D., Rho, Y. S., Kim, N. J. and Kim, J. S. 1990. A study on efficacy of Ulmi cortex. Kor. J. Pharmacogn. 21, 217-222
38 Jung, Y. T., Lee, I. S., Whang, K. and Yu, M. H. 2012. Antioxidant effects of Picrasma quassioides and Chamaecyparis obtusa (S. et Z.) ENDL extracts. J. Life Sci. 22, 354-359.   DOI
39 Kim, H. Y., Lee, Y. J., Kim, S. H., Hong, K. H., Kwon, Y. K., Lee, J. Y., Ha, S. C., Cho, H. Y., Chang, I. S., Lee, C. W. and Kim, K. S. 1999. Studies on the development of natural preservatives from natural products. Kor. J. Food Sci. Technol. 31, 1667-1678.
40 Kim, J. H., Chung, I. K., Kim, H. Y. and Kim, K. M. 2018. Comparison of the quality of dried persimmon (Diospyros kaki THUNB.) treated with medicinal plant extracts and food additives. Food Sci. Nutr. 6, 1991-1998.   DOI
41 Kim, S. J., Shin, J. Y., Park, Y, M., Chumg, K. M., Lee, J. H. and Kweon, D. H. 2006. Investigation of antimicrobial activity and stability of ethanol extracts of licorice root (Glycyrrhiza glabra). Kor. J. Food Sci. Technol. 38, 241-248.