• Korean Journal of Microbiology
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• v.53 no.2
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• pp.71-78
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• 2017

Raman microspectroscopy is a promising tool for microbial analysis at single cell level since it can rapidly measure the cell materials including lipids, nucleic acids, and proteins by measuring the inelastic scattering of a molecule irradiated by monochromatic lights. Using Raman spectra provides high specificity and sensitivity in classification of bacteria at the strain level. In addition, a Raman approach coupled with stabled isotope such as $^{13}C$ and $^2H$ is able to detect and quantify general metabolic activity at single cell level. After bacterial detection process by Raman microspectroscopy, interested unculturable cell sorting and single cell genomics can be accomplished by combination with optical tweezer and microfluidic devices. In this review, the characteristics and applications of Raman microspectroscopy were reviewed and summarized in order to provide a better understanding of microbial analysis using Raman spectroscopy.

• Journal of the Korea institute for structural maintenance and inspection
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• v.7 no.4
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• pp.191-199
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• 2003

The inelastic response of many structural steel and reinforced concrete structures subject to extreme loadings can be characterized by elastoplastic behaviors. Although excursion beyond the elastic range is usually not permitted under normal conditions of service, the extent of permanent damage a structure may sustain when subjected to extreme conditions, such as severe blast or earthquake loading, is frequently of interest to the engineer. A blast is usually the result of an explosion defined as a "sudden expansion". This paper discusses the basic concept that defines blast loadings on structures and corresponding elastoplastic structural response (displacement, velocity, and acceleration) and try to explain a crack propagation of concrete in sudden expansion. According to nonlinear finite element analysis, the crack forms of static and dynamic states displayed different in RC structural members. This paper also provides useful data for the dynamic fracture analysis of RC frame structures.

• Journal of the Earthquake Engineering Society of Korea
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• v.2 no.1
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• pp.79-93
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• 1998

The eigenvalue problem is presented for the building with added viscoelastic dampers by using component mode method. The Lagrange multiplier formulation is used to derive the eigenvalue problem which is expressed with the natural frequencies of the building, the mode components at which the dampers are added, and the viscoelastic property of the damper. The derived eigenvalue problem has a nonstandard form for determining the eigenvalues. Therefore, the problem is examined by the graphical depiction to give new insight into the eigenvalues for the building with added viscoelastic dampers. Using the present approach the exact eigenvalues can be found and also upper and lower bounds of the eigenvalues can be obtained.

• Structural Engineering and Mechanics
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• v.73 no.5
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• pp.529-542
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• 2020

As limited well-documented experimental data are available for assessing the attributes of different deformation components of flanged walls, few appropriate models have been established for predicting the inelastic responses of flanged walls, especially those of asymmetrical flanged walls. This study presents the experimental results for three large-scale T-shaped reinforced concrete walls and examines the variations in the flexural, shear, and sliding components of deformation with the total deformation over the entire loading process. Based on the observed deformation behavior, a simple model based on moment-curvature analysis is established to estimate flexural deformations, in which the changes in plastic hinge length are considered and the deformations due to strain penetration are modeled individually. Based on the similar gross shapes of the curvature and shear strain distributions over the wall height, a proportional relationship is established between shear displacement and flexural rotation. By integrating the deformations due to flexure, shear, and strain penetration, a new load-deformation analytical model is proposed for flexure-dominant flanged walls. The proposed model provides engineers with a simple, accurate modeling tool appropriate for routine design work that can be applied to flexural walls with arbitrary sections and is capable of determining displacements at any position over the wall height. By further simplifying the analytical model, a simple procedure for estimating the ultimate displacement capacity of flanged walls is proposed, which will be valuable for performance-based seismic designs and seismic capacity evaluations.

• Journal of the Earthquake Engineering Society of Korea
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• v.23 no.6
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• pp.289-299
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• 2019

The tendency to use a probabilistic design method rather than a deterministic design method for the design of nuclear power plants (NPPs) will increase because their safety should be considered and strictly controlled in relation to various causes of damage. The distance between a seismically isolated NPP structure and a moat wall is called the clearance to stop. The clearance to stop is obtained from the 90th percentile displacement response of a seismically isolated NPP subject to a beyond design basis earthquake (BDBE) in the probabilistic design method. The purpose of this study is to analyze the effects of heating and buckling effects on the 90th percentile displacement response of a lead-rubber bearing (LRB) subject to a BDBE. The analysis results show that considering the heating and buckling effects to estimate the clearance to stop is conservative in the evaluation of the 90th percentile displacement response. If these two effects are not taken into account in the calculation of the clearance to stop, the underestimation of the clearance to stop causes unexpected damage because of an increase in the collision probability between the moat wall and the seismically isolated NPP.

• Advances in Computational Design
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• v.4 no.4
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• pp.327-342
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• 2019

Rehabilitation and retrofitting of structures designed in accordance to standard design codes is an essential practice in structural engineering and design. For steel structures, one of the challenges is to strengthen the panel zone as well as its analysis in moment-resisting frames. In this research, investigations were undertaken to analyze the influence of the panel zone in the response of structural frames through a computational approach using ETABS software. Moment-resisting frames of six stories were studied in supposition of real panel zone, different values of rigid zone factor, different thickness of double plates, and both double plates and rigid zone factor together. The frames were analyzed, designed and validated in accordance to Iranian steel building code. The results of drift values for six stories building models were plotted. After verifying and comparing the results, the findings showed that the rigidity lead to reduction in drifts of frames and also as a result, lower rigidity will be used for high rise building and higher rigidity will be used for low rise building. In frames with story drifts more than the permitted rate, where the frames are considered as the weaker panel zone area, the story drifts can be limited by strengthening the panel zone with double plates. It should be noted that higher thickness of double plates and higher rigidity of panel zone will result in enhancement of the non-linear deformation rates in beam elements. The resulting deformations of the panel zone due to this modification can have significant influence on the elastic and inelastic behavior of the frames.

• Steel and Composite Structures
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• v.33 no.1
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• pp.19-36
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• 2019

Cyclic behaviour of composite (steel-concrete) plate shear walls (CPSW) with variable column flexural stiffness is experimentally and numerically investigated. The investigation included design, fabrication and testing of three pairs of one-bay one-storey CPSW specimens. The reference specimen pair was designed in way that its column flexural stiffness corresponds to the value required by the design codes, while within the other two specimen pairs column flexural stiffness was reduced by 18% and 36%, respectively. Specimens were subjected to quasi-static cyclic tests. Obtained results indicate that column flexural stiffness reduction in CPSW does not have negative impact on the overall behaviour allowing for satisfactory performance for up to 4% storey drift ratio while also enabling inelastic buckling of the infill steel plate. Additionally, in comparison to similar steel plate shear wall (SPSW) specimens, column "pull-in" deformations are less pronounced within CPSW specimens. Therefore, the results indicate that prescribed minimal column flexural stiffness value used for CPSW might be conservative, and can additionally be reduced when compared to the prescribed value for SPSWs. Furthermore, finite element (FE) pushover simulations were conducted using shell and solid elements. Such FE models can adequately simulate cyclic behaviour of CPSW and as such could be further used for numerical parametric analyses. It is necessary to mention that the implemented pushover FE models were not able to adequately reproduce column "pull-in" deformation and that further development of FE simulations is required where cyclic loading of the shear walls needs to be simulated.

• Structural Engineering and Mechanics
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• v.60 no.6
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• pp.1021-1038
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• 2016

Considerable part of reinforced concrete building has suffered from destructive earthquakes in Turkey. This situation makes necessary to determine nonlinear behavior and seismic performance of existing RC buildings. Inelastic response of buildings to static and dynamic actions should be determined by considering both flexural plastic hinges and brittle shear hinges. However, shear capacities of members are generally neglected due to time saving issues and convergence problems and only flexural response of buildings are considered in performance assessment studies. On the other hand, recent earthquakes showed that the performance of older buildings is mostly controlled by shear capacities of members rather than flexure. Demand estimation is as important as capacity estimation for the reliable performance prediction in existing RC buildings. Demand estimation methods based on strength reduction factor (R), ductility (${\mu}$), and period (T) parameters ($R-{\mu}-T$) and damping dependent demand formulations are widely discussed and studied by various researchers. Adopted form of $R-{\mu}-T$ based demand estimation method presented in Eurocode 8 and Turkish Earthquake Code-2007 and damping based Capacity Spectrum Method presented in ATC-40 document are the typical examples of these two different approaches. In this study, eight different existing RC buildings, constructed before and after Turkish Earthquake Code-1998, are selected. Capacity curves of selected buildings are obtained with and without considering the brittle shear capacities of members. Seismic drift demands occurred in buildings are determined by using both $R-{\mu}-T$ and damping based estimation methods. Results have shown that not only capacity estimation methods but also demand estimation approaches affect the performance of buildings notably. It is concluded that including or excluding the shear capacity of members in nonlinear modeling of existing buildings significantly affects the strength and deformation capacities and hence the performance of buildings.

• Wind and Structures
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• v.28 no.3
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• pp.181-190
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• 2019

Light-frame wood structures have the ability to carry gravity loads. However, their performance during severe wind storms has indicated weakness with respect to resisting uplift wind loads exerted on the roofs of residential houses. A common failure mode observed during almost all main hurricane events initiates at the roof-to-wall connections (RTWCs). The toe-nail connections typically used at these locations are weak with regard to resisting uplift loading. This issue has been investigated at the Insurance Research Lab for Better Homes, where full-scale testing was conducted of a house under appropriate simulated uplift wind loads. This paper describes the detailed and sophisticated numerical simulation performed for this full-scale test, following which the numerical predictions were compared with the experimental results. In the numerical model, the nonlinear behavior is concentrated at the RTWCs, which is simulated with the use of a multi-linear plastic element. The analysis was conducted on four sets of uplift loads applied during the physical testing: 30 m/sincreased by 5 m/sincrements to 45 m/s. At this level of uplift loading, the connections exhibited inelastic behavior. A comparison with the experimental results revealed the ability of the sophisticated numerical model to predict the nonlinear response of the roof under wind uplift loads that vary both in time and space. A further component of the study was an evaluation of the load sharing among the trusses under realistic, uniform, and code pressures. Both the numerical model and the tributary area method were used for the load-sharing calculations.

• Journal of the Korean Geotechnical Society
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• v.34 no.11
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• pp.71-80
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• 2018

In this study, the seismic response of cut-and-cover box tunnels is evaluated from pseudo-static analyses and the fragility curves are derived. A series of site profiles were used to evaluate the effect of soil conditions. A total of 20 ground motions were used. The fragility curves were developed as functions of peak ground acceleration for three damage states, which are minor, moderate, and extensive states. The damage indices, defined as the ratio of the elastic moment to the yield moment, correlated to three damage states, were used. The curves are shown to greatly depend on the site profile. The curves are further compared to those derived in previous studies. The widely used empirically derived curves are shown not to account for the site effects, and therefore underestimate the response for soft sites.