• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.447-459
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• 2010

Bacterial diversity of two distinct wastewater treatment systems, conventional activated sludge (CAS) and membrane bioreactor (MBR), of petroleum refineries were investigated through 16S rRNA gene libraries. Sequencing and phylogenetic analysis showed that the bacterial community composition of sludge samples was distinct between the two wastewater treatment systems. MBR clones belonged predominantly to Class Betaproteobacteria, represented mainly by genera Thiobacillus and Thauera, whereas CAS clones were mostly related to Class Alphaproteobacteria, represented by uncultured bacteria related to Order Parvularculales. Richness estimators ACE and Chao revealed that the diversity observed in both libraries at the species level is an underestimate of the total bacterial diversity present in the environment and further sampling would yield an increased observed diversity. Shannon and Simpson diversity indices were different between the libraries and revealed greater bacterial diversity for the MBR library, considering an evolutionary distance of 0.03. LIBSHUFF analyses revealed that MBR and CAS communities were significantly different at the 95% confidence level ($P{\leq}0.05$) for distances $0{\leq}D{\leq}0.20$. This work described, qualitatively and quantitatively, the structure of bacterial communities in industrial-scale MBR and CAS processes of the wastewater treatment system from petroleum refineries and demonstrated clearly differentiated communities responsible for the stable performance of wastewater treatment plants.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.398-401
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• 2003

Azo dyes are aromatic compounds characterized by one or more azo bonds $(R_l-N=N-R_2)$. More than 800,000 tons of dyes are produced annually worldwide, of which 60-70% are azo dyes. During manufacturing, an estimated 10-15% is released into the environment. Aside from their negative aesthetic effects, certain azo dyes have been shown to be toxic and, in some cases, these compounds are carcinogenic and mutagenic. To establish biological wastewater treatment of azo dye, it is essential to discover azo dye-degrading microorganisms. In this report, sludge-contaminated with dyes were gathered through wastewater outlets from the industrial regions. The following to separation of bacteria within them, bacteria which decolorize methyl red, a azo dye, were selected and destined.

• Economic and Environmental Geology
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• v.33 no.6
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• pp.519-524
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• 2000

This study has been performed to evaluate the possibility of utilizing stone powder sludges from stone quarry and manufacturing plant as adsorbents for heavy metals in industrial wastewater. The stone powder sludges from stone quarry (IS-01) have the most effective adsorption capacity (above 95％ of initial concentrations) under the given experimental conditions of reaction times (Pb : 15 min, Cu : 2 hr, Zn : 48 hr), initial acidity of solution (pH>3) and dosage (sludge/liquid ratio : 0.02). The stone powder sludges from manufacturing plant (CW-01) show relatively high adsorption capacity (about 95％ of initial concentrations) only for Pb with a reaction times of 12 hours, initial acidity of solution (pH>3) and dosage (sludge/liquid ratio : 0.02). The stone powder sludges (IS-01) from stone quarry having relatively excellent adsorption capacity under the given experimental conditions show their potential utilization as heavy metal adsorbents.

• Environmental Engineering Research
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• v.11 no.4
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• pp.173-180
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• 2006

Autotrophic nitrogen removal and its microbial community from a laboratory scale upflow anaerobic sludge bed reactor were characterized with dynamic behavior of nitrogen removal and sequencing result of molecular technique (DNA extraction, PCR and amplification of 16S rDNA), respectively. In the experiment treating inorganic wastewater, the anaerobic granular sludge from a full-scale UASB reactor treating industrial wastewater was inoculated as seed biomass. The operating results revealed that an addition of hydroxylamine would result in lithoautotrophic ammonium oxidation to nitrite/nitrate, and also hydrazine would play an important role for the success of sustainable nitrogen removal process. Total N and ammonium removal of 48% and 92% was observed, corresponding to nitrogen conversion of 0.023 g N/L-d. The reddish brown-colored granular sludge with a diameter of $1{\sim}2\;mm$ was observed at the lower part of sludge bed. The microbial characterization suggests that an anoxic ammonium oxidizer and an anoxic denitrifying autotrophic nitrifier contribute mainly to the nitrogen removal in the reactor. The results revealed the feasibility on development of high performance lithoautotrophic nitrogen removal process with its microbial granulation.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 1998.11a
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• pp.240-244
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• 1998

This study was carried out to investigate the efficiency of steel mill slag and sludge in removing heavy metals and cyanide in metal plating wastewater. Laboratory experiments were peformed with jar tester, The tests were peformed at ambient temperature. The results of tests showed that overall rates metals removed were greater than 90 %. Metals were removed from solution as the combined effects of adsorption and precipitation in alkaline condition. The removal efficiency of cyanide by steel mill wastes was above 90 % at optimum conditions. In view of the test results and other engineering characteristics of steel mill slag and sludge, these industrial by-products from steel industry have a high potential to be used in metal plating wastewater treament and were particularly beneficial.

• Applied Chemistry for Engineering
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• v.24 no.4
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• pp.423-427
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• 2013

This research was carried out to identify the characteristics of the wastewater from coke oven gas (COG) purification process of the coke plant, and derive optimal operating conditions for the treatment of wastewater. The coke plant wastewater contains highly concentrated $S^{-2}$ and $SCN^-$ that are harmful to microorganisms, and their concentrations were 6.8~11.2 mg/L and 190~320 mg/L, respectively. When the $S^{-2}$ ion concentration was lower than 10 mg/L, $SV_{30}$ of active sludge was 280~ 340 mL and the sludge sedimentation velocity was very fast. But, when the $S^{-2}$ ion concentration was higher than 15 mg/L, $SV_{30}$ of the active sludge was 560~680 mL and the sludge sedimentation velocity was very slow. Also when the $SCN^-$ ion concentration was lower than 300 mg/L, $SV_{30}$ of the active sludge was 245~320 mL and the sludge sedimentation velocity was very fast. But, when the $SCN^-$ ion concentration was higher than 400 mg/L, $SV_{30}$ of the active sludge was 470~ 567 mL and the sludge sedimentation velocity was slow. To treat the wastewater generated by COG purification process of the coke plant effectively and to maintain microorganism activities in good conditions, the ion concentration of $S^{-2}$ and $SCN^-$ should be lower than 15 mg/L and 400 mg/L, respectively.

• KSBB Journal
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• v.25 no.1
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• pp.103-107
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• 2010

Oil degradation agent was developed with organic sludge and modified peat moss (MPM) to recover oil contaminated soil. Waste sludge discharged from wastewater treatment plant of chemical plant in Ulsan National Industrial Park was used as organic sludge, and MPM was purchased. Organic sludge was adequate to use as growth medium for microorganism, the surface of MPM had porous structure which could enhance the cultivation condition of oil degradation microorganisms. Water contents and TPH variation with time were observed to investigate the degradation capacity of developed degradation agent. Water contents were rapidly decreased with higher contents of MPM, however, in case of TPH, high MPM content decreased the degradation capacity. Therefore, it was recommended that the content of MPM was controlled to below 10% in degradation agent as mixing organic sludge with MPM.

• Clean Technology
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• v.13 no.2
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• pp.87-98
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• 2007

As the interest in environmental problems increased, the guideline of effluent qualities becomes strict and the amount of sludge produced from the wastewater treatment facilities steeply increased. The revised Korean acts prohibit the direct reclamation of the sludge exhausted in sewage treatment works from 2003, and the ocean dump of sludge will be also prohibited from 2012. Therefore, the sludge must be recycled, incinerated or composted. To properly perform the utilization and disposal operations, the establishment of well-defined procedures for the characterization of sludge by physical, chemical and biological method and setting up of guidelines for different treatment and disposal routes has become necessary. However, there have not been such standardized methods for sludge characterization in Korea. This review analyzes the present status of establishment and problems of sludge characterization method of Korea. Finally, it was proposed total 15 items of standardized characterization methods suitable to our country for proper sludge recycling and disposal, which was based on the more systematic standard methods of Germany (DIN 38414) and those of advanced nations.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.10
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• pp.1090-1097
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• 2006

The anoxic activated sludge process was applied to the treatment of industrial box-mill wastewater, which exhibited the high removal efficiencies of $90{\sim}94%$$ TCOD_{Mn}$ and $58{\sim}81%$ Color. For the design of industrial anoxic activated sludge process, Monod bio-kinetic coefficients of box-mill wastewater were estimated as follows: $K_{max}$(maximum specific substrate removal rate)=0.52 $day^{-1}$, $K_s$(half saturation constant)=314 mg/L, $K_d$(decay coefficient)=0.274 $day^{-1}$, y(microbial yield coefficient)=0.908 mg/mg, and ${\mu}_{max}$(maximum specific growth rate)=0.472 $day^{-1}$. Space loading factors for the design analysis were practically determined as the values of F/M ratio=$0.043{\sim}0.07$ kg-$TCOD_{Mn}$/kg-SS-day, BOD space loading=$0.18{\sim}0.3$ kg-$TCOD_{Mn}/m^3-day$, and ${\theta}_x=6.8{\sim}26.4$ day when considering the relationship of these loading factors with growth dynamics of microorganisms, the F/M ratio that is inversely proportional to ${\theta}_x$ should be equivalent to ${\mu}_{max}$ in units, but exhibited the significant difference between theses two values. Therefore, it is considered that high safety factors are requested in the design of anoxic activated sludge process that is based on Monod bio-kinetics of microorganism.

• Environmental Engineering Research
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• v.23 no.1
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• pp.28-35
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• 2018

$17{\alpha}-ethinylestradiol$ (EE2), a synthetic estrogen which interfere the endocrine and reproductive function in living organisms, has been found extensively to be deposited into municipal wastewater treatment plants and the environment via human excretion. EE2 has long been known to be efficiently cometabolized by ammonia-oxidizing bacteria (AOB) during ammonia ($NH_3$) oxidation. Current study aims to investigate the effect of culture history on the biotransformation of EE2 by nitrifying sludge which was enriched under different ammonia loading rates in continuous flow reactors. Result showed that past growth condition largely affected not only the metabolic rate of $NH_3$ oxidation but also EE2 cometabolism. Sludge previously acclimated with higher $NH_3$ loads as well as sludge dominated with AOB belong to high growth cluster (Nitrosomonas europaea-Nitrosococcus mobilis) showed higher rate of EE2 biotransformation than those one being acclimated with lower $NH_3$ loads because of its ability to provide more reducing power from $NH_3$ oxidation. Moreover, the correlation between the degradation rates of $NH_3$ and EE2 was higher in sludge being acclimated with higher load of $NH_3$ in comparison with other sludge. Implication of the findings emphasized the role of volumetric $NH_3$ loading rate in determining EE2 removal in wastewater treatment system.