• International Journal of Industrial Entomology and Biomaterials
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• v.45 no.2
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• pp.49-55
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• 2022

A total of 20 mulberry varieties preserved in Korea were typed for eight polymorphic microsatellite loci. We obtained 6 to 15 alleles per locus with an average value of 10.6, per-locus observed heterozygosity ranging from 0.35 to 1.00, and per-locus polymorphic information content (PIC) ranging from 0.61 to 0.87, indicating that most loci are highly variable. Phylogenetic analysis using the eight microsatellite loci was sufficiently suitable for classifying 20 mulberry varieties preserved in Korea. A total of 160 variety-specific apomorphic alleles were obtained from eight loci discriminated 20 mulberry varieties. These variety-specific alleles from this analysis are expected to be useful for the discrimination of other mulberry varieties. Furthermore, a substantial number of homozygote loci, represented by 60 among 180 alleles in eight loci were found. These results collectively suggest that these microsatellite locus primers are potentially crucial molecular markers for the eventual classification of mulberry varieties that are preserved as hundreds in Korea.

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.2
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• pp.126-133
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• 2023

Genetic resources of mulberry trees are commonly preserved as trophosomes, which are vulnerable to environmental factors, such as natural disasters, diseases, and pests. This study establishes a basic protocol for ultra-low temperature cryopreservation of mulberry trees using a two-step freezing process. The procedure was established using the "Daeshim" variety and then tested on genetic resources from 24 other mulberry varieties. Samples were first dried to a moisture content of 33-43% in a low-temperature forced-air chamber at -5 ℃, then slowly frozen from -5 ℃ to -20 ℃, and preserved in liquid nitrogen (-196 ℃). To determine the regeneration rate, isolated dormant buds were inoculated into MS basal medium, and grown shoots were grafted onto 1-year-old rootstock via chip budding and then cultured. After freezing in liquid nitrogen, the "Daeshim" variety exhibited a survival and regeneration rate of more than 70% and 50%, respectively. Applying the two-step freezing process to genetic resources from 24 mulberry species yielded average survival and regeneration rates of 85.3% and 75.5%, respectively. Morus alba showed survival and regeneration rates of 100%, confirming the efficacy of the two-step freezing method. These results indicate the high feasibility of ultra-low-temperature cryopreservation through two-step freezing of dormant buds from mulberry genetic resources. Additional research is required into the variations in regeneration rates with freezing period in liquid nitrogen.

• Journal of Environmental Science International
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• v.31 no.11
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• pp.981-987
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• 2022

This study aimed to investigate the psychological effect of a healing agricultural program using insect resources and to confirm the persistence of the healing effect. The applied insect was silkworm Bombyx mori (Linnaeus) and 28 adolescents from local children's centers were used as the subjects. The healing agricultural program was applied at the centers for 4 weeks, and stress was measured using salivary α-amylase levels and a survey on life satisfaction, happiness, and insect preference. The results indicated that stress levels decreased, and life satisfaction and insect preference increased after participating in the the healing agricultural program. Furthermore, it was confirmed that this healing effect continued until 4 weeks after the end of the program.

• International Journal of Industrial Entomology and Biomaterials
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• v.31 no.2
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• pp.70-74
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• 2015

The current market size of insect industry in Korea is estimated at 300 million dollars and more than 500 local farms are related to many insect industry. One of the strong candidates for insect industry is Korean horn beetle, Allomyrina dichotoma. Early this year, we reported a viral disease extremely fatal to A. dichotoma larvae. While we were proceeding a nationwide investigation of this disease, it was informed that similar disease symptom has been occurred occasionally during past over 10 years. The symptom can be easily confused with early stage of bacterial infection or physiological damage such as low temperature and high humidity. A peroral infection with the purified virus to healthy larvae produced a result that only 21% of larvae survived and became pupae. Although some of the survived adult beetle was deformational, many of them had no abnormal appearance and even succeeded in mating. Later, these beetles were examined if they were carrying the virus, and all except one were confirmed as live virus carrier. This implies that these beetles may fly out and spread the disease to the nature. We found the evidence for this possibility by collecting a few wild A. dichotoma larvae which were virus infected, near two local farms rearing A. dichotoma larvae. So far, transovarial transmission of this virus to the eggs, or horizontal transmission to other commercially reared insects is not known yet.

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.2
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• pp.59-62
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• 2016

Many insects have gained increasing attention as an alternative protein for humans. Among those, the two-spotted cricket, Gryllus bimaculatus (Orthoptera: Gryllidae), was recently approved as a general food ingredient by the Korean Ministry of Food and Drug Safety. For industrial utilization of G. bimaculatus, mass rearing techniques and production system should be standardized first. In this study, we investigated the effects of five different feeds on the growth and development of G. bimaculatus. Feed is the one of the key factors that has considerable effects on rearing insects. With five different kinds of feed on $1^{st}$, $3^{rd}$, and $5^{th}$ instar nymphs, the change of survival rate, body weight were monitored up to eight wk after hatching. We concluded that 50% of soybean flour, 20% of corn powder, 10% of rice bran, 9% of milk serum, 10% of rice flour, 0.5% of microorganisms, and 0.5% of multivitamins and minerals (diet C) was the best mix for promoting growth and development of $3^{rd}$ instar nymphs compared to the control diet.

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.1
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• pp.44-50
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• 2023

The Daehwangjam has excellent marketability and quality. However, precise silkworm breeding data on the recent high-temperature environment is not enough collected, and it is necessary to check whether stable Daewhangjam silkworm egg production was possible in poor environments. In this study, the timing of metamorphosis of the Daewhangjam parents Jam323 and Jam324 silkworms, in an optimal condition and a general condition similar to breeding grounds, was investigated to confirm that appropriate mating was achieved. Surprisingly, a number of female moths were discarded due to the metamorphosis of Jam324 female moths much earlier than Jam323 male moths in either an optimal or a general condition. To improve this, during the Jam324 female pupation period, the mild low-temperature of 15℃ was treated for the pupation period to delay the metamorphosis into female moths. Depending on the treatment period, the metamorphosis of the female pupa was delayed by more than 4 days, allowing normal mating with the Jam323 male moth. The eye pigments in a pupa also slowly accumulated in these treatments. In order to discover the side effects of low-temperature treatments, the voltinism of the Daewhangjam eggs was investigated, but most of them remained univoltine. Taken together, the low-temperature treatment during the female pupation period made it possible to use Jam324 female moths that were should be discarded, which means that stable and efficient management of Daewhangjam egg production is possible.

• KSBB Journal
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• v.30 no.6
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• pp.257-267
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• 2015

Insects are the most successful organisms on earth in terms of their diversity and adaptability. Insect biotechnology using this insect resource is an emerging area for future biotechnology with various applications. Insect resources have long been used to make food and/or functional food, feed, cosmetics as well as medicine and industrial ingredients. Recently, one of the most well-known industrial material from insect is spider silk that could be commercialize in near future. The insect cell lines have been used to express recombinant proteins that were difficult to be functional expression. For public purpose, while, the insect could be good amenity source and plant farming, so leisure resource. Only the interdisciplinary research will guarantee the successful story for insect biotechnology. And biochemical engineers should used insect as a bioresource for new products with applications in medicine, agriculture, and industrial biotechnology in near future. This review will cover state-of-the art of this field and the research and application areas of insect biotechnology and the possible role of biochemical engineer for the development of the future biotechnology using this bioresource.

• International Journal of Industrial Entomology and Biomaterials
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• v.35 no.2
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• pp.100-105
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• 2017

Under natural conditions, black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), colonizes in warm temperate regions, and is active in Korea from May through October. Information on black soldier fly rearing, which is affected by seasonal factors in Korea, is limited. Oviposition by black soldier flies is dependent on light intensity and wavelength. Therefore, continuous mass rearing of this fly requires determination of optimal artificial conditions of illumination. In this study, we compared the number of eggs laid under an artificial light source (750 watt HPL lamp) versus nature sunlight. Our results showed that compared to oviposition under natural sunlight, the use of one or two lamps for 7 hours, resulted in only 43 and 76%, of the total number of eggs laid under natural sunlight, respectively. We also investigated the hatchability of oviposited eggs under artificial illumination and under natural sunlight. The hatching rate under the former was much significantly lower than under the latter. Further detailed research is required to develop methods for successful mass rearing of black soldier fly throughout the year by means of an indoor system.

• International Journal of Industrial Entomology and Biomaterials
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• v.45 no.1
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• pp.22-28
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• 2022

Silkworms are very sensitive to changes in temperature and humidity, and unless it is a suitable temperature and humidity to grow, the productivity and quality of silkworms are greatly reduced. Therefore, it is very important to manage temperature and humidity for silkworm feeding facilities. In particular, it is essential to install heating facilities in Asian countries with distinct seasonal changes. During the feeding period, many farms manage the temperature and humidity of feeding facilities by installing and using electric heaters inside the facilities. However, it is very difficult to manage the room temperature stably by the heaters. In addition, unlike the temperature inside the facility, silkworms could undergo severe temperature changes as the inside of the rearing tray could not be warm enough. In this study, in order to improve the previous heating method, the new rearing method that directly heats the bottom of the rearing tray was developed. Compared to the previous room-heating system, the novel heater-installed tray (HIT) system significantly reduced the change in temperature during the experimental period. In addition, the number of days of silkworm growth up to harvest was shortened, which was effective in growth performance, and it was also found that silkworms grew more uniformly in HIT system than in previous system. Moreover, as the heater tubes were installed directly under the rearing tray, it quickly dried mulberry leaves and silkworm feces after feeding, and as a result, the environment in the tray was greatly improved with decrease the labor of breeder. In conclusion, these results suggest that the heater-installed rearing tray method greatly improves silkworm quality, increases weight of silkworms, and final profits compared to the previous room heating system with electric heaters.

• Korean journal of applied entomology
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• v.59 no.3
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• pp.169-175
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• 2020

We developed an evaluation index for selecting pet insect species. As an expert opinion survey, the Delphi method was used to establish evaluation items. Using AHP (analytic hierarchy process), evaluation criteria for selecting pet insects were proposed. And then based on application testing, we established a scoring system.