• Korean Journal of Agricultural Science
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• v.40 no.3
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• pp.197-202
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• 2013

This study was aimed to find the storage potential of low temperature adapted shiitake mushroom under freezing temperature ($-3^{\circ}C$). Fresh shiitake mushroom was harvested at late Feb. and cooled to target temperature, $-3^{\circ}C$ in the cooling room for 24 hr. Cooled mushroom were then placed in plastic boxes, wrapped with plastic film ($30{\mu}m$), and stored at $-3^{\circ}C$ for 2 months. Weight loss of mushroom was ranged from 2.1 to 3.2%. Dry weight per unit fresh weight, however, was slightly increased because of moisture loss. Firmness of fruit body increased from $0.95kg/cm^{-2}$ (before storage) to $1.13kg/cm^{-2}$ (after 2 month storage). About half amount of starch was lost during 2 month storage. The amount of total and reducing sugars remained relatively constant. After storage, freshness of mushroom was recovered by thawing treatment. When recovered mushroom were packaged with styrofoam tray and PVC wrapping, and exposed to ambient and $10^{\circ}C$, respectively, brown spot on the gill of fruit body was found and slight decay symptom was also found at ambient temperature only but not at $10^{\circ}C$. Results indicated that low temperature adapted shiitake mushroom has a storage potential under freezing temperature ($-3^{\circ}C$). Freezing storage technology of fresh shiitake mushroom will contribute the increase of storability up to 2 months.

• Journal of Microbiology and Biotechnology
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• v.28 no.8
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• pp.1391-1400
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• 2018

Angelica tenuissima root has historically been used as a traditional medicine in Korea. Previous studies have identified the anti-melanogenic effects of the extract of A. tenuissima root fermented by Aspergillus oryzae (FAT). This study investigated the protective effects of FAT against ultraviolet light B exposure (UVB; $30mJ/cm^2$) in HaCaT (human keratinocyte) or Hs68 (human foreskin fibroblast) skin cells. FAT treatment was able to stimulate wound healing rate at the basal condition. FAT also favored the maintenance and/or improvement of extracellular matrix impairment caused by UVB irradiation through: 1) upregulation of procollagen Type-1 synthesis and secretion; 2) suppression of MMP-1 and elastase expression. FAT was able to play a role in the attenuation of inflammatory responses caused by UVB irradiation via upregulation of photo-protective hemeoxygease-1 and suppression of proinflammatory cyclooxygenase-2 expression. After further verification of the anti-photoaging potential of FAT, it could be utilized as an effective ingredient in anti-aging and anti-wrinkle cosmetics.

• Journal of Digital Convergence
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• v.18 no.3
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• pp.171-177
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• 2020

Under the influence of the Fourth Industrial Revolution, there are many tries to promote productivity enhancement and competitiveness by adapting smart farm technology that converges ICT technologies in agriculture. This smart farming technology is emerging as a new paradigm for future growth in agriculture. The development of real-time cultivation environment monitoring and automatic control system is needed to implement smart farm. Furthermore, the development of intelligent system that manages cultivation environment using monitoring data of the growth of crops is required. In this paper, a fast and efficient development method for implementing a cloud-based smart farm management system using a highly compatible and scalable web platform is proposed. It was verified that the proposed method using the web platform is effective and stable system implementation through the operation of the actual implementation system.

• Korean Journal of Plant Resources
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• v.30 no.3
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• pp.272-279
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• 2017

EP was obtained through 20% ethanol extraction of Pueraria lobata root, and the fermented form of EP, FEP, was prepared from the EP after incubating with Lactobacillus rhamnosus vitaP1. There was no significant toxicity by EP and FEP up to $1000{\mu}g/ml$ in NIH-3T3, HaCaT, and B16F10 cells. In addition to antioxidant potentials of EP and FEP determined by DPPH and ABST assays, we confirmed increase of procollagen type I and elastin synthesis by supplementation of the EP and FEP at the concentration of $50{\mu}g/ml$ using ELISA kits. The protein expression levels of matrix metalloprotease (MMP)-1, -3, and -9, those are involved in the degradation of collagen or other skin matrix proteins, were remarkably suppressed while their inhibitory protein metallopeptidase inhibitor 1 (TIMP-1) was greatly up-regulated by supplementation of the EP and FEP at a concentration of $50{\mu}g/ml$. Taken together, both EP and FEP supplementation could be involved in the suppression of the skin wrinkle formation through inhibiting degradation of collagen and stimulating the synthesis of collagen and elastin. The results showed that the anti-wrinkle potential of the EP and FEP will be a promising candidate for developing cosmeceutical compounds or products.

• International Journal of Industrial Entomology and Biomaterials
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• v.28 no.2
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• pp.51-57
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• 2014

Bumblebees are important pollinators of crops and wildflowers. The Korean native bumblebee, Bombus ignitus, undergoes one generation per year, and induction of artificial hibernation is essential for year-round rearing of the bumblebee. Keeping queens under cold treatment conditions for several mo is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how the timing and duration of chilling affect the artificial hibernation of B. ignitus queens. In the timing assessment, cold treatment was instituted at 12 d, 40 d, or 100 d after eclosion under a constant temperature of $5^{\circ}C$ and 80% humidity. The queens that entered cold treatment at 12 d after emergence evidenced the highest survival rates: 86.7% at two mo, 73.3% at three mo, and 46.4% at 4 mo. Survival rates were reduced under storage conditions at 12 d, 40 d, and 100 d after emergence. When queens were subjected to chilling at 8 d, 12 d, or 16 d after eclosion with constant 80% humidity, the queens stored at 12 d after eclosion exhibited the highest survival rates, which were 84.6 at one mo, 25.0% at two mo, and 7.9% at three mo. In regards to the duration of the cold period, the queens that hibernated for at least two mo evidenced optimal colony development rates. The rates of oviposition, colony foundation, and progeny-queen production of queens hibernated for two mo were 60.0%, 30.0%, and 13.3%, respectively. These values were 6.0 to 13.3 times higher than those in the queens that hibernated for 15 d. Therefore, a cold period of at least 2 mo applied 12 d after emergence were found to be the most favorable conditions for diapause break in B. ignitus queens.

• International Journal of Industrial Entomology and Biomaterials
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• v.27 no.2
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• pp.219-227
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• 2013

The purpose of this study was to quickly and effectively identify the Nosema disease of bumblebees in Gangwon Province in Korea. Bumblebees are crucial pollinators of various crops, and microsporidia are the critical pathogens of these hosts. When bumblebees are infected with Nosema bombi, their abdomens can become distended. Paralyzed and infected workers often become sluggish and die early. We have identified the morphology of the microsporidium by light and electron microscopy, and found it to have fairly small oval spores, as has been described previously in many other articles. For the specific and sensitive diagnosis of the microsporidian parasite N. bombi in bumblebees, we have developed an improved method of the polymerase chain reaction (PCR) for expeditious diagnosis. Two pairs of primers were tested on N. bombi and the related microsporidia Nosema apis and Nosema sp., both of which infect Bombus ignitus and Bombus hypocrita sapporoensis. Furthermore, we have verified and analyzed the 16SrRNA sequence data of N. bombi in bumblebees by using the Basic Local Alignment Search Tool (BLAST) server at the National Center for Biotechnology Information.

• The Plant Pathology Journal
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• v.33 no.4
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• pp.362-369
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• 2017

White root rot disease, caused by the pathogen Rosellinia necatrix, is one of the world's most devastating plant fungal diseases and affects several commercially important species of fruit trees and crops. Recent global outbreaks of R. necatrix and advances in molecular techniques have both increased interest in this pathogen. However, the lack of information regarding the genomic structure and transcriptome of R. necatrix has been a barrier to the progress of functional genomic research and the control of this harmful pathogen. Here, we identified 10,616 novel full-length transcripts from the filamentous hyphal tissue of R. necatrix (KACC 40445 strain) using PacBio single-molecule sequencing technology. After annotation of the unigene sets, we selected 14 cell cycle-related genes, which are likely either positively or negatively involved in hyphal growth by cell cycle control. The expression of the selected genes was further compared between two strains that displayed different growth rates on nutritional media. Furthermore, we predicted pathogen-related effector genes and cell wall-degrading enzymes from the annotated gene sets. These results provide the most comprehensive transcriptomal resources for R. necatrix, and could facilitate functional genomics and further analyses of this important phytopathogen.

• International Journal of Industrial Entomology and Biomaterials
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• v.20 no.2
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• pp.93-98
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• 2010

Bumblebees are widely used to pollinate various crops, especially tomato, in greenhouses and fields. An artificial hibernation is essential for year-round rearing of the bumblebee, which passes through one generation per year. Here, we investigated whether a chilling temperature and humidity affect artificial hibernation of the bumblebee queen Bombus terrestris. In chilling temperature regimes of $0^{\circ}C$, $2.5^{\circ}C$, $5^{\circ}C$, $7.5^{\circ}C$ or $12.5^{\circ}C$ under constant humidity >70%, the queens stored at $2.5^{\circ}C$ exhibited the highest rate of survival, which was 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months, and 24.0% at 6 months. Rates of survival decreased at the following temperatures: $0^{\circ}C$, $5^{\circ}C$, $7.5^{\circ}C$ and $12.5^{\circ}C$. Colony developmental characteristics after diapause were 1.2- to 1.5-fold higher than those of queens stored at $5^{\circ}C$. In terms of chilling humidity, the queens hibernated at 70% under $2.5^{\circ}C$ exhibited the highest rate of survival, which was $93.3{\pm}3.4%$ at one month, $83.3{\pm}0.0%$ at two months, $76.7{\pm}0.0%$ at 3 months and $36.7{\pm}12.1%$ at 5 months. The rates of oviposition, colony foundation and progeny-queen production of queens hibernated at 70% were 80.8%, 30.8% and 30.8%, respectively. These values correspond to 1.7- to 3.3-fold increases in comparison to queens stored at 50% humidity. Therefore, $2.5^{\circ}C$ and 70% R.H. were the favorable chilling temperature and humidity conditions for diapause break of B. terrestris queens.

• International Journal of Industrial Entomology and Biomaterials
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• v.29 no.1
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• pp.135-144
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• 2014

Rearing of tropical tasar silkworm, Antheraea mylitta Drury is mainly conducted in outdoor on Terminalia tomentosa W. & A. a nature grown primary host plant available in forest and also on raised primary host plant Terminalia arjuna Bedd. Temperature, relative humidity and rainfall are the main environmental factors for occurrence of pests (parasites and predators) of tasar silkworm during I, II and III crop rearing in the tropical tasar producing zones. The present study was aimed to study the influence of abiotic factors on prevalence of tasar silkworm pests. The study was conducted at different agro-climatic regions viz., Central Tasar Research &Training Institute, Ranchi, Jharkhand, Regional Extension Centre, Katghora, Chattisgarh and Regional Extension Centre, Hatgamaria during 2010-13 covering 3 seed crop and 6 commercial crops. Data on incidence of tropical tasar silkworm endo-parasitoids like Uzi Fly, Blepharipa zebina Walker and Ichneumon fly (Yellow Fly), Xathopimpla pedator, Fabricius and Predators such as Stink bug (Eocanthecona furcellata Wolf), Reduviid bug (Sycanus collaris Fabricius) and Wasp (Vespa orientalis Linnaeus) was recorded Weekly. The meteorological data was collected daily. Data was collected from 4 different agro-climatic zones of tasar growing areas. Analysis of the data revealed a significant negative correlation between abiotic factors and incidence of ichneumon fly and uzi fly. Based on the 3 years data on prevalence of pests region-wise pest calendars and prediction models were developed.

• The Korean Journal of Mycology
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• v.26 no.3 s.86
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• pp.321-326
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• 1998

Two strains, each of Tremella fuciformis and Hypoxylon sp. were isolated and their mycelial properties were investigated. T. fuciformis produced yeast-like conidia and dikaryotic mycelia from white to yellow color. The diameter of the hyphae was $1.5\;to\;3.0\;{\mu}m$, and septa with clamp connections were present. Secondary mycelia with clamp connections could produce fruitbodies on sawdust medium when the environmental conditions were suitable for fructification. The symbiotic fungus, Hypoxylon sp. produced white feather-like mycelia. But the color of old mycelia was changed to light yellow or light brown and pigmented the culture medium from light brown to brown or very dark green. Generally it did not produce conidia, but in a special case it produced conidia of which color was yellow green to grass green and of which shape was sub-elliptical with the size of approximately $3\;to\;5\;{\mu}m$.