Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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The Suppressive Effect of Pueraria lobata Root Extract and Its Biotransformed Preparation against Skin Wrinkle Formation

  • Koo, Hyun Jung (Department of Medicinal and Industrial Crops, Korea National College of Agriculture and Fisheries) ;
  • Lee, SungRyul (Department of Integrated Biomedical Science, Cardiovascular and Metabolic Disease Center, Inje University) ;
  • Kang, Se Chan (Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Kwon, Jung Eun (Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Lee, Da Eun (Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Choung, Eui-Su (DanjoungBio Co., Ltd.) ;
  • Lee, Jong-Sub (DanjoungBio Co., Ltd.) ;
  • Lee, Jin Woo (Department of Herbal Medicine Resources, Kangwon National University) ;
  • Park, Yuna (Department of Herbal Medicine Resources, Kangwon National University) ;
  • Sim, Dong Soo (Department of Herbal Medicine Resources, Kangwon National University) ;
  • Sohn, Eun-Hwa (Department of Herbal Medicine Resources, Kangwon National University)
  • Received : 2017.04.07
  • Accepted : 2017.06.21
  • Published : 2017.06.30
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Abstract

EP was obtained through 20% ethanol extraction of Pueraria lobata root, and the fermented form of EP, FEP, was prepared from the EP after incubating with Lactobacillus rhamnosus vitaP1. There was no significant toxicity by EP and FEP up to $1000{\mu}g/ml$ in NIH-3T3, HaCaT, and B16F10 cells. In addition to antioxidant potentials of EP and FEP determined by DPPH and ABST assays, we confirmed increase of procollagen type I and elastin synthesis by supplementation of the EP and FEP at the concentration of $50{\mu}g/ml$ using ELISA kits. The protein expression levels of matrix metalloprotease (MMP)-1, -3, and -9, those are involved in the degradation of collagen or other skin matrix proteins, were remarkably suppressed while their inhibitory protein metallopeptidase inhibitor 1 (TIMP-1) was greatly up-regulated by supplementation of the EP and FEP at a concentration of $50{\mu}g/ml$. Taken together, both EP and FEP supplementation could be involved in the suppression of the skin wrinkle formation through inhibiting degradation of collagen and stimulating the synthesis of collagen and elastin. The results showed that the anti-wrinkle potential of the EP and FEP will be a promising candidate for developing cosmeceutical compounds or products.

Keywords

References

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