• BMB Reports
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• v.33 no.2
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• pp.184-187
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• 2000

Prostaglandin $E_2$ ($PGE_2$) plays an important role in the regulation of various gastric functions, and the growth-inhibitory activities on tumor cells are studied in vitro and in vivo. Although the mechanisms have attracted many researchers in the past decade, the molecular mechanisms of cell cycle arrest, or induction of apoptosis by $PGE_2$, is unclear. We investigated the effects of $PGE_2$ on the growth of the human gastric carcinoma cell line SNU1 and genes that are regulated by $PGE_2$ and isolated them using differential display RT-PCR (DD RT-PCR). FACS analysis suggested that SNU1 cells were arrested at the G1 phase by $PGE_2$ treatment. This growth inhibitory effect was in a time- and dose-dependent manner. Treatment of SNU1 cells with $10\;{\mu}g/ml$ $PGE_2$, followed by DD RT-PCR analysis, revealed differently expressed bands patterns from the control. Among the differently expressed clones, we found an unidentified cDNA clone (HGP-27) overexpressed in $PGE_2$-treated cells. The full-length cDNA of HGP-27 was isolated using RACE, which consisted of a 30-nt 5'-noncoding region, a 891-nt ORF encoding the 296 amino acid protein, and a 738-nt 3'-noncoding region including a poly(a) signal. This gene was localized on the short arm of chromosome number 11. Using the Motif Finder program, a myb-DNA binding repeat signature was detected on the ORF region. The COOH-terminal half was shown to have similarity with the $NH_3$-terminal domain of thioredoxin (Trx). This relation between HGP-27 and Trx implied a potential role for HGP-27 in modulating the DNA binding function of a transcription factor, myb.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.23 no.1
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• pp.141-149
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• 2009

In this paper, quasi-static FDTD method is implemented by FORTRAN programming, and it is used for analysis of body induced current in middle frequencies. The quasi-static FDTD program is validated by comparing the calculation result with analytic solution of the test model, to which it is difficult to apply conventional FDTD. It is confirmed that the time-step is reduced by $5.68{\times}10^6$ times. Using validated numerical technique, body induced current distribution in high resolution 3-D human model is calculated for 20[kHz] magnetic field exposure and 1[MHz] electric field exposure. Also, the effect of grounding condition of both feet on the distribution and amplitude of the induced current is analyzed. It is expected that this research can be applied to various fields including safety assessment of body induced current and development of diagnosis devices using bio-electricity.

• Clinical and Experimental Reproductive Medicine
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• v.21 no.1
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• pp.13-20
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• 1994

Status of endometrium is a very important factor which influences the implantation of fertilized embryos. In this study, we evaluated the possibility that the endometrial depth and pattern assessed by vaginal sonography on the day of human chorionic gonadotropin (HCG) injection in in vitro fertilization (IVF) cycles could be used to predict the IVF outcome. A total of 112 cycles using gonadotropin releasing hormone agonist (GnRHa) for ovulation induction were evaluated. We classified all patients into group A(<9mm) or group B(${\geq}$ 9mm) according to endometrial depth, and into group l(hyperechogenic), group 2(isoechogenic) or group 3(hypoechogenic and triple line) according to endometrial pattern. The other classification was made considering both endometrial depth and pattern. There was no significant correlation between serum estradiol level and endometrial sonographic findings(depth and pattern)(p>0.05). The pregnancy rate of group A(31.3%) did not differ significantly from that of group B(43.7%), but no pregnancies were found in any patients with endometrial depth less than 6mm. The pregnancy rate was 40%, 35.7%, and 44.6 % for group 1, gorup 2, and group 3, respectively, but there was no statistically significant difference between these groups(p>0.05). In combined classification, there was a trend of higher pregnancy rate in case of endometrial depth greater than 9mm and hypoechogenic triple line pattern, but there was no statistically significant differences between these groups(p>0.05). The conclusion from the present data is that endometrial ultrasonography on the day of hCG administration had no predictive value for conception in IVF cycles.

• The Journal of the Acoustical Society of Korea
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• v.21 no.2
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• pp.134-141
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• 2002

In this paper, a free wake analysis based on the curved vortex element and CVC wake model is used to predict the aerodynamic performance and noise for HAWT. Also for prediction of RPM, a maximum value through a quadratic regression was suggested. And for a noise prediction, the broadband noise prediction method based on experimental equation was used. The curved vortex element uses a BCVE and an SIVE instead of a straight vertex element. In the CVC wake model, the vortex strengths are assumed to be constant along a span and a vortex filament. The free wake structure made by the curved vortex element and CVC was substituted for a vortex lattice, so it has an advantage for the less calculation time and a depiction of accurate wake structure. For the verification of this program, calculated results are compared with Mr. Kim's experiment model and Zond Z-40FS for performance and with WTS-4 and USWP models for noise. Good agreements are obtained between the predicted and the measured data for the performance and far-field noise spectra.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.37 no.3
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• pp.36-42
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• 2014

Domestic 105mm HE (High Explosive) shell is composed of three parts that are Fuze, Projectile and Propellants. Among three parts, propelling charge of propellants part consists of single base propellants. It has been known that the lifespan of single base propellants is affected by a storage period. These are because Nitrocellulose (NC) which is the main component of propelling gunpowder can be naturally decomposed to unstable substances similar with other nitric acid ester. Even though it cannot be prevented fundamentally from being disassembled, a decomposition product ($NO_2$, $NO_3$, and $HNO_3$) and tranquillizer DPA (Diphenylamine) having high reactivity are added into a propellant to restrain induction of automatic catalysis by a decomposition product. The decay rate of the tranquillizer is also affected by a production rate of the decomposition product of NC. Therefore, an accurate prediction of the Self-Life is required to ensure against risks such as explosion. Hereupon, this paper presents a new methodology to estimate the shelf-life of single base propellants using data of ASRP (Ammunition Stockpile Reliability Program) to domestic 105mm HE (propelling charge of propellants part). We selected four attributes that are inferred to have influence on distribution of the DPA amount in a propellant from the ASRP dataset through data mining processes. Then the selected attributes were used as independent variables in a regression analysis in order to estimate the shelf-life of single base propellants.

• The Korea Journal of Herbology
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• v.31 no.1
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• pp.25-31
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• 2016

Objectives : The present study was conducted to evaluate protective effects of Combined Extract of young persimmon fruit and citrus peel (PCM) in Reflux Esophagitis(RE) rats.Methods : Twenty-four Sprague-Dawley (SD) rats were divided four groups and each group had six rats ; Normal group, RE control group, RE group treated PCM 50 ,100 mg/kg body weight group. Reflux esophagitis was induced that tied the pylorus and fundus in SD rats stomach. PCM was administered at 50, 100 mg/kg body weight 2 hrs prior to induction of RE. After 6 hrs, the effects of PCM treated rats were compared with those of normal and control rats. We have performed an analysis such as pH of stomach secretion, oxidative stress biomarkers in serum, and western blot.Results : The increased esophageal mucosa damage by RE was markedly improved by PCM treatment in a dose-dependent manner. Also, the administration of PCM decreased the elevated serum reactive oxygen species (ROS) and peroxynitrite (ONOO^-) in serum. The protein expressions of anti oxidant such as SOD, catalase, GPx exhibited down-regulation by PCM treatment in tissues. And, PCM effectively reduce inflammatory cytokines such as inflammation-related proteins cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) in RE rats. In addition, NFκB and p-IκBɑ were decreased in PCM-adiministrated RE rats. But there was no difference on stomach secretion pH between reflux esophagitis rats and PCM administration rat group.Conclusions : In conclusion, administration of PCM (50, 100 mg/kg body weight) made esophagus have less inflammation and injury by decreased NFκB path way. These findings suggest that PCM could have Improving effects on reflux esophagitis.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.15
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• pp.6513-6519
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• 2015

Background: Recent attention on chemotherapeutic intervention against cancer has been focused on discovering and developing phytochemicals as anticancer agents with improved efficacy, low drug resistance and toxicity, low cost and limited adverse side effects. In this study, we investigated the effects of Curcuma C20-dialdehyde on growth, apoptosis and cell cycle arrest in colon and cervical cancer cell lines. Materials and Methods: Antiproliferative, apoptosis induction, and cell cycle arrest activities of Curcuma C20-dialdehyde were determined by WST cell proliferation assay, flow cytometric Alexa fluor 488-annexin V/propidium iodide (PI) staining and PI staining, respectively. Results: Curcuma C20 dialdehyde suppressed the proliferation of HCT116, HT29 and HeLa cells, with IC50 values of $65.4{\pm}1.74{\mu}g/ml$, $58.4{\pm}5.20{\mu}g/ml$ and $72.0{\pm}0.03{\mu}g/ml$, respectively, with 72 h exposure. Flow cytometric analysis revealed that percentages of early apoptotic cells increased in a dose-dependent manner upon exposure to Curcuma C20-dialdehyde. Furthermore, exposure to lower concentrations of this compound significantly induced cell cycle arrest at G1 phase for both HCT116 and HT29 cells, while higher concentrations increased sub-G1 populations. However, the concentrations used in this study could not induce cell cycle arrest but rather induced apoptotic cell death in HeLa cells. Conclusions: Our findings suggest that the phytochemical Curcuma C20-dialdehyde may be a potential antineoplastic agent for colon and cervical cancer chemotherapy and/or chemoprevention. Further studies are needed to characterize the drug target or mode of action of the Curcuma C20-dialdehyde as an anticancer agent.

• Korean Journal of Environmental Biology
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• v.38 no.2
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• pp.323-331
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• 2020

The method of natural spawning is very passive and inconvenient for the study of developmental engineering in marine medaka, Oryzias dancena. The optimum concentration of human chorionic gonadotropin (HCG) and carp pituitary extract (CPE) for ovulation and spawning, and the injection time for the artificial spawning of marine medaka were analyzed in this study. The success rate, survival rate, and hatching rate were highest with 100IU HCG kg^-1 BW and 5mg CPE L^-1 in both male and female marine medaka (p<0.05). After obtaining unfertilized eggs and sperm by the injection of HCG and CPE into the broodstock of marine medaka, artificial fertilization could be successfully achieved any time fertilized eggs are needed in this species. This result should be useful for developing a study program for marine medaka as an experimental animal.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.14 no.5
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• pp.95-102
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• 2014

In this paper, we propose the high speed PLC-based automatic control system for a smart LED streetlight. The proposed the automatic control system were constructed of a power line modem part and monitoring part, streetlight controller part for the high speed communication frequency band and streetlight ballasts characterization and real-time remote control using a high-speed PLC network, and it was designed to meet to lighting grades conditions of KS road lighting standards. The proposed automatic control system were easy monitoring of the power consumption using PC through to the comparison result of the existing streetlight system. As a result, it was confirmed to the possibility of efficient operation for the real-time monitoring and maintenance by induction of reasonable power consumption through to the LED streetlight state checking and remote-control. In addition, we proved to improvement of expected effects for the power cost savings, the energy efficiency, and streetlight differentiation and advanced.

• Korean Institute of Interior Design Journal
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• v.24 no.2
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• pp.125-133
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• 2015

Gaze induction characteristics in space vary depending on characteristics of spatial components and display. This study analyzed dominant eye-fixation characteristics of three zones of department store space. Eye-fixation characteristics depending on spatial components and positional relationship can be defined as follows. First, [**.jpg] was used as an extension in the process of storing the image photographed during image data processing for analysis in pixels and due to compressed storage of image data, the image produced with a clear boundary was stored in neutral colors. To remove this problem, the image used in operation was re-processed in black and white and stored in the [**.bmp] format with large capability, at the same time. As the result, the effort caused by unnecessary colors in the program operation process was corrected. Second, the gaze ratio to space area can be indicated as a strength of each gaze zone and when analyzing the gaze strength of the three zones, the left store was a zone with a "little strong" gaze strength of "102.8", the middle space was a zone with an "extremely weak" gaze strength of "89.6" and the right store was a zone with an "extremely strong" gaze strength of "117.2". Third, the IV section had a strong strength of gaze on the middle space and the right store and the V section showed a markedly strong strength of gaze on the left and right stores. This tendency was the same as the VI section with the strongest gaze strength and the right store had a little strong gaze strength than the left store.