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http://dx.doi.org/10.11626/KJEB.2020.38.2.323

Artificial induction of spawning by human chorionic gonadotropin and carp pituitary extract in marine medaka, Oryzias dancena  

Park, In-Seok (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University)
Publication Information
Korean Journal of Environmental Biology / v.38, no.2, 2020 , pp. 323-331 More about this Journal
Abstract
The method of natural spawning is very passive and inconvenient for the study of developmental engineering in marine medaka, Oryzias dancena. The optimum concentration of human chorionic gonadotropin (HCG) and carp pituitary extract (CPE) for ovulation and spawning, and the injection time for the artificial spawning of marine medaka were analyzed in this study. The success rate, survival rate, and hatching rate were highest with 100IU HCG kg-1 BW and 5mg CPE L-1 in both male and female marine medaka (p<0.05). After obtaining unfertilized eggs and sperm by the injection of HCG and CPE into the broodstock of marine medaka, artificial fertilization could be successfully achieved any time fertilized eggs are needed in this species. This result should be useful for developing a study program for marine medaka as an experimental animal.
Keywords
artificial fertilization; carp pituitary extract; human chorionic gonadotropin; marine medaka;
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Times Cited By KSCI : 14  (Citation Analysis)
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