• Title/Summary/Keyword: induction period

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Controlled Lysis of Lipase-Producing Recombinant E. coli by Phage Induction (Lipase를 생산하는 재조합 대장균의 phage에 의한 조절적 용균)

  • 문윤희;구윤모
    • KSBB Journal
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    • v.10 no.5
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    • pp.575-581
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    • 1995
  • A plasmid pTTY2, containing the lipase-producing gene, was used to transform an E. coli phage lysogen, P90c/$\phi$434, into the lipase-producing lysogen, P90c/$\phi$434/pTTY2. After the overproduction of lipase by the isopropylthio-${\beta}$-D-galactoside induction, the prophage $\phi$434 in the chromosome of the host cell was induced by the milomycin C addition or ultraviolet irradiation to lyse the host cell. The optimum operating conditions, such as the isopropylthio-${\beta}$-D-galactoside induction period and the phage induction timing, were sought for the efficient cell lysis in the same fermenter. Effective cell lysis occurred at the earlier exponential growth phase with the isopropylthio-${\beta}$-D-galactoside induction period of 1 hour. The amount of the lipase production was qualitatively measured by the halo size in Luria-Bertani agar medium containing tributyrin and Rhodamine B plate.

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A Study on the Digital Control of Single Phase Induction Motor Driven by the Full Bridge Resonant Inverter(I) - The Current Characteristics of Full - bridge Resonant Inverter at Low Frequency - (전브리지 공진형 인버터에 의한 단상 유도전동기의 디지탈제어에 관한 연구(I) -저주파수에서 전브리지 공진형 이버터의 전류특성에 대하여-)

  • 노영오;박진길
    • Journal of Advanced Marine Engineering and Technology
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    • v.17 no.3
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    • pp.70-79
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    • 1993
  • The application of A.C. motor to servo system is rapidly increased according to the recent advance of power electronics and digital control techniques. The induction motor which has a simple structure and needs less maintenance is used in the industrial field for the variable speed and position control recently. In this paper, the current characteristic of the full-bridge resonant inverter is studied by comparing with the computer simulation and the laboratory experiment when the ratio of forced frequency to the natural frequency and the ratio of conduction time to the period at the given frequency is changed. And then, the full-bridge resonant inverter is applied to the speed control of single phase induction motor.

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The Effects of EGR and EGR Induction Point on Combustion Noise of a Passenger Diesel Vehicle (승용 디젤엔진의 EGR과 Induction위치에 따른 소음 영향)

  • Kang, Sang-Kyu;Kim, Jae-Heon;Baek, Sung-Nam;Kang, Koo-Tae
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2007.11a
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    • pp.393-396
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    • 2007
  • EGR is well established and efficient means to reduce NOx emissions. The increase of EGR rate affects the ignition delay of the combustion due to the lower oxygen availability. The increasing of the ignition delay period causes large combustion noise. In this study, the effects of EGR and Induction Point on combustion noise are investigated by measuring cylinder pressure and noise. As a result, The Combustion noise is markedly increased under the application of EGR. The increased premixed distance by displacing EGR Induction point in flow direction causes the uniform EGR distribution and the modulation level of the combustion noise is reduced slightly.

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Induction Heating of a Billet for Semi-Solid Forging (반용융 단조를 위한 소재의 유도 가열)

  • 최재찬;박형진;김병민
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1997.04a
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    • pp.670-674
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    • 1997
  • Semi-solid forging is a compound forging technology to deventional forging process. Among several steps of semi-solid forging process, the heating step of a billet prior to semi-solid forging step is necessarily required to obtain globular microstructure. For the forming operation to work properly, it is also important to heat the billet uniformly for the uniformity of solid-liquid distribution. To satisfy these requirements, induction heating has been generally used for a long time. This paper presents the method to find heating condition and the temperature distribution inside of a billet with a induction heating apparatus by comparing the computer simulation with experiment for aluminum alloys Al2024 and A356.

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Induction Heating of a Billet for Semi-Solid Forging (반용융 단조를 위한 소재의 유도 가열)

  • Park, J.C.;Park, H.J.;Kim, B.M.
    • Journal of the Korean Society for Precision Engineering
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    • v.14 no.8
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    • pp.15-20
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    • 1997
  • Semi-solid forging is a compound forging technology to develop conventional forging process. Among several steps of semi-solid forging process, the heating step of a billet prior to semi-solid forging step is necessarily required to obtain globular microstructure. For the forming operation to work properly, it is also important to heat the billet uniformly for the uniformity of solid-liquid distribution. To satisfy these requirements, induction heating has been generally used for a long time. This paper presents the method to find heating condition and the temperature distribution inside a billet with a induction heating apparatus by comparing the computer simulation with experiment for aluminium alloys A12024 and A356.

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Flower-Inducing Activity in the Phloem Exudata and Gene Expression Specific to Photoperiodic Floral Induction in Pharbitis Cotyledons

  • Kim, Kang-Chang;Lee, Jin-Hwan;Her, Yoon-Kang;Maeng, Jue-Son
    • Journal of Plant Biology
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    • v.39 no.4
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    • pp.257-263
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    • 1996
  • Flower-inducing activity in the phloem exudata of Pharbitis cotyledons was investigated using the bioassay of Pharbitis and Lemna. By SDS-PAGE and 2-D gel electrophoresis of the phloem exudate, two polypeptides of 11 kDa and of approximately 32 kDa (pI 6.9) showing qualitative changes during the flower induction were detected. A polypeptide of approximately 20 kDa (pI 4.8) specifically labeled in vivo with [35S]methionine was found during the inductive dark period in Pharbitis cotyledon tissues. The polypeptide of the equivalent molecular mass and with the identicl pI value was also detected by in vitro translation assay. Thus, it is assumed that the 20 kDa polypeptide plays a role in the process of flower induction in Pharbitis cotyledons.

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Purification and Utilization of Industrial Waste Water Using Microorganism -(Part 1) Isolation of the yeast strain from organic waste water and its use on waste water treatment- (산업폐수의 처리 및 이용에 관한 연구 -(제 1 보) 효모균주의 분리와 이에 의한 유기성폐수의 처리에 관하여-)

  • Lee, Kang-Heup;Yim, Sung-Sam;Park, Tai-Won
    • Applied Biological Chemistry
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    • v.20 no.2
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    • pp.228-235
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    • 1977
  • The yeast strain was isolated from food industry waste water and its identification and biological characteristics were investigated. The optimum condition for cultivations and its activities for the reduction of B.O.D. on the food industry waste water were also confirmed. The results are as follows; 1) The isolated was identified as Candida curvata. 2) Candida curvata grew well in all of the experimented media, so and it can be regarded as a useful strain in the treatment of food industry waste water. 3) There was only a slight difference in the induction period between sterilized cultivation and unsterilized cultivation. But in the ice cream waste water, the period was considerably longer in unsterilized cultivation. 4) Specific rate of growth of Candida curvata in sugar waste water was 0.50/hr, ice cream waste water 0.50/hr, and beer waste water 1.0/hr. 5) Increasing of innoculum reduced the induction period in unsterilized cultivation. 6) The amount of dried yeast from sugar waste water were $175mg/{\ell}$, ice cream waste water $628mg/{\ell}$, and beer waste water $857mg/{\ell}$. Crude protein content in the dried yeast from sugar waste water were 52%, ice cream waste water 54%, and beer waste water 54%. 7) The rate of BOD reduction in sugar waste water were 49%, ice cream waste water 80%, and beer waste water 64%.

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Improvement of Reproductive Efficiency of Artificial Insemination Following Estrus Induction in Dog I. Estrus Induction and Changes of Progesterone and Estrogen in Dog

  • Lee, Y.R.;S.Y. Choe
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.91-91
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    • 2002
  • Considerable attention has been focused on the cryopreservation of semen and estrus induction in dog, as consequence of poor productivity caused by long anestrus period, in order to enhance the productivity of youngs and to preserve the breeds. The objectives of this study were to develop a treatment protocol for estrus induction. Fifty infertilie dogs (age 2∼3 years) were selected for the study and divided into three different estrus induction treatment groups. Group 1: dogs (n=15) were given clomifane (0.1 mg/kg) orally for five days at 12 hr intervals. Group 2: dogs (n=15) were given bromocriptine (50 $\mu\textrm{g}$/kg) orally for five days at 12 hr intervals, followed by single injection intravenously of 500 IU GnRH (Group 3, n=20) when pro-estrus occurred. After being treated, the dogs were evaluated for the rates of estrus induction and time interval lapses from treatment to beginning of the pro-estrus. The estrus induction rates were significantly (P<0.05) higher in both group 2 (9/15, 73.3%) and group 3 (16/20, 80.0%) than that of group 1 (9/15, 60.0%), but did not differ in the groups 2 and 3. No differences were observed in the time interval lapses from treatment to beginning of the pro-estrus in group 2 (7.7 ${\pm}$ 1.2 days) and group 3 (6.9 ${\pm}$ 2.0 days), but significantly (P<0.05) shorter than that of group 1 (9.5 ${\pm}$ 2.1 days). In conclusion, the estrus induction rate of dogs treated with a combination of GnRH and bromocriptine was more effective than use of clomifene or bromocriptine only.

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Simple analysis on induction motor dynamic performances by time constant parameter (유도전동기의 동특성해석에 있어서의 Time constant parameter에 의한 간이해석법)

  • 황영문
    • 전기의세계
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    • v.31 no.2
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    • pp.126-131
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    • 1982
  • Induction motors are known to cause voltage dip, oscillating torque and inrush current on the dynamic period. To compensate for these undesirable effects, the prediction of dynamic performances is required. The dynamic performances are determinated by circuit time constants. From this point of view, in this paper, the dynamic equivalent circuit included only three time-constant parameters are presented. To predict more simply dynamic performances, the new characteristics time constant parameters are analyzed, and now these parameters are described as the function of circuit time constants. This paper reviews and analyzes the use of series capacitance compensations, and the use of this analysis can make simply a prediction about oscillating conditions.

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Studies on Inhibition Factors and the Role of Phytochrome in the Floral Induction in Short-day Plants (단일식물의 개화유도를 조절하는 개화억제요소의 규명과 Phytochrome의 역할에 관한 연구)

  • 맹주선
    • Journal of Plant Biology
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    • v.19 no.1
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    • pp.14-18
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    • 1976
  • Inhibition of flowering in Lemna perpusilla 6746 by 30 mM sucrose was reversed by the addition of acetylcholine (>$10^{-4}M) supplemented with 10^{-4}M$ ascorbic acid to 1/10-strength Hunter's growth medium. The reversible effect of acetylcholine was found to be greater at early stages of flowering than in the later period. Promotive effects of both acetylcholine ($10^{-3}M) and eserine(10^{-5}M$) on flowering in the short-day plant under various photoperiodic conditions were studied. It was indicated that the application decreased length of the critical dark period for the floral induction, and it was also shown that the endogenous status of acetylcholine was involved in the floral response which had a correlation with phytochrome. Interruption of inductive dark periods by red irradiation (1min) immediately followed by far-red light (1 min) completely inhibited flowering, while the addition of acetylcholine and eserine to the medium under the same condition slightly promoted flowering, indicating possible involvement of phytochrome system in acetylcholine activity for photoperiodic sensitivity of floral response in Lemna perpusilla 6746.

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