• 한국초지조사료학회지
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• 제9권3호
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• pp.124-128
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• 1989

本實驗은 Vernal알팔파의 callus 誘導에 미치는 몇가지 要因들에 對하여 糾明하고자 逐行되었으며 얻어진 結果는 다음과 같다, Callus 誘導에서 auxin 源으로서 2,4-D를 2-5mg/1 單用處理한 것이 가장 좋았으며, 여러가지 基本 培地 中에서는 B5와 SH가 callus 誘導에 가장 效果的이었는데, PC와 MS 培地에서 生成된 callus는 friable 하였다. 培地#의 pH는 5.8이 가장 좋았으며 7.0 以上에서는 callus는 거의 생장하지 않았다. 組織片은 9日 齡된 植物體의 것이 가장 좋았으며, 이를 前後하여 callus의 生成量은 減少하였다. Callus 誘導時, 光條件의 有無는 큰 影響을 나타내지 않았으나, 빛 條件에서는 callus 組織은 푸르게 되었다.

• 아시안잔디학회지
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• 제11권4호
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• pp.311-320
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• 1997

This study was carried out to induce and maintain callus from 59 zoysiagrass lines, to know the effective disinfestation method for zoysiagrass stolon as explant and the difference in the response of callus induction among 59 lines, and to investigate the effect of medium, growth regulators, light, temperature, stolon part and internode position on callus induction and emhryogenic callus(E.C.) formation. The treatment of 0.lmg/L $HgCl_2$for 15 min resulted in no contamination and the highest callus induction(46.6%). Callus was induced from the 59 zoysiagrass lines. The callus growth of Z. japonica and Z. sinica was generally better than Z. matrella Ten cell lines whose callus and stolon grow fast in culture and in field, respectively were selected to he used for breeding. Callus induction was the most effective at 2.0mg /L of both 2, 4-D and picloram in MS medium. MS medium was the best for callus induction and growth while LS medium was the best for embryogenic callus and shoot formation. Callus induction and growth was better at 28, 31$^{\circ}C$. than 25$^{\circ}C$. and dark condition was better than light condition in MS me-dium containing 2mg/L 2,4-D. While callus induction was better with node part as explant than with internode part, callus growth and embryogenic callus formation was better with internode part. In 'Japonica 1', the first internode was the most effective in callus induction, but third internode was the best in '$M_2$ X $S_2$'.

• 한국작물학회지
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• 제47권5호
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• pp.352-355
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• 2002

Mature embryos of five oat genotypes were cultured to develop an efficient method of callus induction and plant regeneration. Murashige and Skoog(MS) and N6 media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin were used for callus induction. Percentage of callus induction showed significant among the combinations of plant growth regulators. Callus induction showed high efficiency in medium containing 3 mg/$\ell$ of 2,4-D. The high frequency of callus induction was obtained in Gwiri37. For plant regeneration, calli induced from mature embryos were transferred onto MS and N6 media supplemented with combinations of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA) for 5 weeks. Percentage of plant regeneration showed high in MS medium containing 0.2 mg/$\ell$ of NAA and 1 mg/$\ell$ of BA. The callus initiation medium affected the subsequent plant regeneration. Treatment with 3 mg/$\ell$ of 2,4-D, and 3 mg/$\ell$ of 2,4-D and 3 mg/$\ell$ of kinetin in callus induction media showed high frequency for plant regeneration. Plant regeneration frequency among the genotypes showed significant. Especially, Gwiri37 showed high regeneration frequency. Regenerated shoots were treated with 200, 350 and 500 mg/$\ell$ of indole-3-butyric acid (IBA) transferred onto half-strength MS medium without plant growth regulators. Treatment of shoots with IBA induced root formation rapidly.

• The Korean Journal of Ecology
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• 제16권3호
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• pp.275-285
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• 1993

To study allelopathic porential of naturally occurring substances emitted from Pinus koraiensis, the effects of water exrracts and volatile substances of the tree on callus induction and bacterial culture were examined. The induction and growth of callus were inversely proportional to the concentration of the extracts and of the essential oils supplemnted to Murashige and Skoog's media. However, low concentration(5μl/l) of essential oil promoted callus growth. In germiculture of several bavteria, the extracts of Pinus koraiensis markedly showed the inhibitory effects on bacterial growth. It was, therefore, confirmed that the KDICical substances of Pinus koraiensis tree clearly showed that biological toxic activity at high concentrations.

• 한국작물학회지
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• 제30권3호
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• pp.271-276
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• 1985

벼 약배양에 있어서 기본배지 및 배지내 첨가물질이 callus유기와 식물체분화에 미치는 영향을 구명하기 위하여 시험한 결과는 다음과 같다. 1. callus 유기 및 식물체분화는 N$_{6}$기본배지에서 가장 높았으며 Indica형 품종보다는 Japonica형 품종에서 그 효율이 더욱 높게 나타났다. 2. 약치상후 callus유기는 기본배지의 종류에 관계없이 20~40일 사이에 90% 이상이 유기되었다. 3. 식물체분화는 callus유기배지의 영향을 크게 받으며 Miller 배지에서 유기된 callus의 식물체분화는 N$_{6}$나 MS배지에 비하여 저조하였다. 4. N$_{6}$배지내에 포함된 질소원중 (NH$_4$)$_2$SO$_4$의 함량을 31.5mM로 조절하였을 때 callus 및 식물체분화율이 향상되었다. 5. 생장조정제 첨가에 있어 2,4-D단독 처리보다는 NAA 2mg/$\ell$에 Kinetin 1mg/$\ell$를 혼용 처리하였을 때 callus유기 뿐 아니라 식물체분화율도 향상되었다. 6. Indica형 품종의 callus유기에 대한 DL-Ala-nine 첨가효과는 약을 저온처리하였을 때 높게 나타났다.

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.257-262
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• 2006

The paper evaluated the behavior of in vitro culture responses from a diverse set of Indica rice (Oryza sativa L.) genotypes. Significant differences were found in embryogenic callus induction frequency, callus growth and plant regeneration frequency when mature embryos of 11 cultivars, breeding lines and land races were compared. Genotype as well as plant growth regulator influenced the plant regeneration frequency. Callus induction frequency was not correlated with callus growth as well as plant regeneration frequency. The regenerated plants could grow to normal, fertile plants after they were successfully established in soil.

• Journal of Plant Biotechnology
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• 제36권3호
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• pp.236-243
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• 2009

The halophyte Leymus chinensis (Trin.) is a perennial rhizome grass (tribe Gramineae) that is widely distributed throughout China, Mongolia and Siberia. This study was conducted to investigate an optimal condition for plant regeneration from mature seeds, leaf base segments, and root segments in L. chinensis. Plant growth regulators affecting embryogenic callus induction and plant regeneration were investigated by four-factor-three-level [L9 (34)] orthogonal test in this study. The effects of explants types (mature seeds, leaf base segments and root segments), callus types, medium types were examined in this study. Wild type (WT) and Jisheng No. 1 plants (JS) were used for primary callus induction. A clear explants difference was seen during callus induction; mature seeds were considered as the preferred explants; and the highest frequency of callus induction was obtained in Medium 6 using mature seeds as explants in WT. Plant regeneration ability was evaluated by frequencies of green callus forming, shooting, rooting, and shooting with roots. Effect of α-naphthalene acetic acid (NAA) on shoot regeneration was remarkable with the highest frequency of 70.8% in WT after 2-month culture. The medium with 0.2- 0.5 mg/L NAA was found to have the highest shoot induction. All regenerated shoots were successfully rooted when transferred on half-strength Murashige and Skoog (MS) basal medium. The acclimatized plantlets were grown to mature with flowering and seeds setting in green house conditions.

• 한국작물학회지
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• 제29권3호
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• pp.232-241
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• 1984

수도 약배양의 효율을 높이기 위하여 callus 유기와 녹색체 출현율에 영향하는 화분의 발육단계, 화분의 저온처리와 기관 및 배지의 영향에 대한 일련의 실험을 수행하였으며 이에서 얻어진 결과를 요약하면 다음과 같다. 1. 단핵기의 화분에서 callus 유기율이 높았으며 이때는 대체로 엽신간장이 5~8cm에 해당되나 품종에 따라 차이가 있다. 2. 이삭을 상, 중, 하로 나누어 조사한 결과 엽이간장이 5~8cm의 것은 상부와 중간부분에 단핵기에 해당되는 것으로 나타났고 9~10cm 정도의 것은 중부와 하부 부분이 단핵기에 해당하는 것으로 나타났다. 3. 일반적으로 저온처리는 8$^{\circ}C$에서 12$^{\circ}C$가 callus 유기에 알맞는 것으로 나타났다. 그러나 온도에 대한 반응은 품종에 따라 차이가 있어 4$^{\circ}C$에서 callus 유기가 잘된 것도 있었다. 4. 저온 처리기간은 일반적으로 8~12일 정도에서 callus 유기가 잘 되었으나 품종간 차이가 있다. 5. 본 실험에서는 N6D 배지보다 N6 배지에서 callus 유기율이 높았다. 6. 대체로 녹색체 출현율은 4$^{\circ}C$와 8$^{\circ}C$로 처리한 것이 12$^{\circ}C$ 처리보다 높았다. 그러나 품종간 차이가 있었다. 7. 녹색체 출현에 좋은 결과를 주었던 온도 처리기간은 대체로 8~12일로 나타났다. 8. 본 실험에서 녹색체 출현율은 N6 배지보다 개량 MS 배지에서 2배 정도 높았다.

• 원예과학기술지
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• 제29권5호
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• pp.488-493
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• 2011

Callus culture initiation of strawberry (Fragaria${\times}$ananassa Duch.) was investigated at different Murashige and Skoog (MS) medium strengths, types and concentrations of plant growth regulators, and incorporating a cold pretreatment period to determine the optimal nutritional and environmental conditions. No high quality callus was induced on MS media without auxin regardless of medium strength. When 6-benzylaminopurine (BA) was combined with indole acetic acid (IAA), naphthalene acetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D), high quality callus were highly induced compared to medium supplemented with auxin alone. When $0.5mg{\cdot}L^{-1}$ BA was combined with IAA, NAA, and 2,4-D, high quality callus induction was more effective than the medium supplemented with the other BA concentrations. The best combination of auxin and cytokinin for high quality callus induction was $1.0mg{\cdot}L^{-1}$ NAA and $0.5mg{\cdot}L^{-1}$ BA. Although the differences in callus induction were not significant, high quality callus induction at half strength MS medium was more effective than at full strength medium. When $30g{\cdot}L^{-1}$ sucrose was added to the half strength MS medium, the rate of high quality callus induction increased. The optimum cold pretreatment temperature and period for high quality callus induction were $4^{\circ}C$ and 72 h, respectively. Regeneration rate of high quality callus increased in MS medium supplemented with thidiazuron.

• 생명과학회지
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• 제11권4호
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• pp.340-345
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• 2001

Effects of growth regulators(2,4-D, IAA, NAA, BA and kinetin) and chilling treatment on callus induction. embryogenesis and regeneration through anther cultures of B. falcatum L. were examined. Frequency of callus induction and embryogenesis was effectively increased by the treatment of chilling at 5$^{\circ}C$ for 10 days before anther inoculation. Optimal level of growth regulator for callus induction and embryogenesis from anther was 2,4-D 1.0 mg/L in Murashige and Skoog(MS) basal medium supplemented with 30 g/L sucrose, 8 g/L agar. Frequency of embryogenesis from anther derived callus was increased up to 48% or 45% by addition of IAA 0.1+ kinetin 1.0 mg/L of IAA 0.1+ BA 1.0 mg/L in MS medium, respectively, Optimal medium for obtaining green callus was MS basal supplemented with BA 1.0mg/L. Addition of auxins(IAA or NAA) inhibited the formation of green callus from anther derived callus.