• Nutritional Sciences
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• 제9권1호
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• pp.20-28
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• 2006

Aryl sulfotransferase (AST) IV is a liver enzyme involved in detoxication and has been shown to be susceptible to down regulation by a number of hepatotoxic xenobiotics. Studies presented here to investigate the ability of biological and non-biological divalent metal cations on AST IV activity showed that AST IV was strongly inhibited following in vitro or in vivo exposure to. Zn ( II ), Co ( II ) or Cd ( II ). It was found that $0.025\sim$2.5 uM of these metal ions were sufficient to cause $50\%$ of inhibition in vitro in purified AST IV and $0.25\sim$25 uM of these metal ions in liver cytosolic fractions. For the in vivo study, 1,000 mg Cu ( II )/kg, 2,000 mg Zn ( II )/kg or 250 mg Cd( II )/kg was added to individual diets and administered to three (3) group; of mts over a 7 week period The Co ( II )-supplemented diet produced no apparent change in rat growth rate and resulted in 30-fold increase in liver cytotolic Cu ( II ) levels, suggesting that elevated levels of Cu ( II ) ion in the liver were responsible for the loss of AST IV activity. In contrast, the Zn ( II )-supplemented diet caused a decrease in rat growth rates and resulted in zero increase in liver Zn ( II ) levels, which suggested an indirect inhibition mechanism was caused by Zn ( II ) in the liver. Rats were fed the Cd-supplemented diet also displayed a decrease in growth rate with little or no change in liver Cu ( II ) or Zn ( II ) levels. When the liver cytosols of mts from the metal ion diets were immunochemically analyzed for the AST IV and albumin contents, no significant changes were observed in albumin levels. However, AST IV contents in the cytosols of mts fed the Zn ( II )-supplemented diets showed a slight decrease in amount These results showed that AST IV activity in vitro and in vivo can be inhibited by Co ( II ), Zn ( II ), and Cd ( II ) by apparently different mechanisms. The immediate response to a Zn injection showed a decrease in AST IV activity but not in the AST IV content in liver cytosol. These mechanisms appeared to involve direct actions of the metal ion on AST IV activity and indirect actions affecting AST IV amount.

• 유통과학연구
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• 제9권3호
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• pp.55-64
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• 2011

본 연구는 소아비만 치료제 시장에 있어 중요한 마케팅 대상그룹인 구매 타켓, 주부들을 대상으로 한 설문조사를 통해 소아비만 시장에서의 구매의사 결정요인과 유통경로 결정요인 등을 분석해 효과적인 브랜딩 전략을 수립하기 위한 것이다. 최근 비만이 사회적으로 큰 문제로 떠오르고 있고 몇 몇 연구들을 통해 우리나라의 외식문화로부터 사회적 비만문제의 원인을 찾는 경우가 있어 외식프랜차이즈 산업에 있어서도 비만 문제는 관심 있게 바라보고 대처해야 할 관심사라 할 수 있다. 특히 소아비만의 경우 소아비만 환자의 80~85%가 성인비만으로 이어져 평생 비만과 씨름하면서 살아야 하는 심각한 문제를 야기 한다. 따라서 이러한 소아비만 문제는 부모들의 소비행동에 적잖은 영향을 미치고 있다. 이에 본 연구에서는 소아비만 시장의 구매의사 결정요인과 유통경로 결정요인 등을 살펴보고 이를 바탕으로 한 효과적인 브랜딩 전략과 브랜드 마케팅 커뮤니케이션 전략수립 방안을 제시하여 소아비만 치료제 시장 활성화에 기여하고 나아가 소아비만 문제해결에 일조하기 위함이다.

• 한방안이비인후피부과학회지
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• 제23권1호
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• pp.199-214
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• 2010

Objective : Atopic dermatitis (AD) may profoundly affect patient's quality of life (QOL), and also cause economic impact. The aim of our study was to evaluate the quality of life and the economic impact of adult atopic dermatitis patient in Seoul. We also evaluate the relationship of QOL and economic impact with severity of AD. Methods : 30 adult atopic dermatitis patient were included and evaluated by using the SCORAD Index and EASI. Patients were asked to fill in the questionnaires about their quality of life and financial costs during the past year by AD. Data about sleep disturbance and pruritus were also obtained. Pearson's correlation were used for statistical analysis. Results : 1. Among 30 patients, women were 19(63.3%), men were 11(36.7%). The mean age of the patients were 27.3 years old, patients between the ages of 17 and 30 years were 23, over 30 were seven. 2. The mean score of Objective SCORAD was $32.89{\pm}7.30$, Subjective SCORAD was $8.13{\pm}3.53$ and EASI was $9.15{\pm}6.90$이었다. 3. The mean score of Skindex-29 was $28.26{\pm}7.58$, DLQI was $10.17{\pm}5.55$. 4. By analyzing the questionnaire, a monthly average cost of 583,200 won for each patient was determined. Direct cost was 236,800 won and indirect cost was 346,300 won. 5. By analyzing the correlation between the severity of AD and QOL, Objective SCORAD and EASI were positively correlated with QOL(Skindex-29, DLQI) but not significant, meanwhile subjective SCORAD were significantly and positively correlated with QOL(Skindex-29, DLQI). 6. By analyzing the correlation between the severity of AD and economic impact, Objective SCORAD were significantly and positively correlated with direct cost and indirect cost. Also, EASI were significantly and positively correlated with direct cost and oriental medical hospital visits. Conclusions : The above results show that the QOL of the patients with atopic dermatitis is significantly related to their disease severity. Atopic dermatitis patients pay 583,200 won a month, and the economic impact of the patients is significantly related to their disease severity.

• Journal of Veterinary Science
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• 제23권4호
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• pp.55.1-55.12
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• 2022

Background: ASF was first reported in Kenya in 1910 in 1921. In China, ASF spread to 31 provinces including Henan and Jiangsu within six months after it was first reported on August 3, 2018. The epidemic almost affected the whole China, causing direct economic losses of tens of billions of yuan. Cause great loss to our pig industry. As ELISA is cheap and easy to operate, OIE regards it as the preferred serological method for ASF detection. P54 protein has good antigenicity and is an ideal antigen for detection. Objective: To identify a conservative site in the African swine fever virus (ASFV) p54 protein and perform a Cloth-enzyme-linked immunosorbent assay (ELISA) for detecting the ASFV antibody in order to reduce risks posed by using the live virus in diagnostic assays. Method: We used bioinformatics methods to predict the antigen epitope of the ASFV p54 protein in combination with the antigenic index and artificially synthesized the predicted antigen epitope peptides. Using ASFV-positive serum and specific monoclonal antibodies (mAbs), we performed indirect ELISA and blocking ELISA to verify the immunological properties of the predicted epitope polypeptide. Results: The results of our prediction revealed that the possible antigen epitope regions were A23-29, A36-45, A72-94, A114-120, A124-130, and A137-150. The indirect ELISA showed that the peptides A23-29, A36-45, A72-94, A114-120, and A137-150 have good antigenicity. Moreover, the A36-45 polypeptide can react specifically with the mAb secreted by hybridoma cells, and its binding site contains a minimum number of essential amino acids in the sequence 37DIQFINPY44. Conclusions: Our study confirmed a conservative antigenic site in the ASFV p54 protein and its amino acid sequence. A competitive ELISA method for detecting ASFV antibodies was established based on recombinant p54 and matching mAb. Moreover, testing the protein sequence alignment verified that the method can theoretically detect antibodies produced by pigs affected by nearly all ASFVs worldwide.

• 의료법학
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• 제22권2호
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• pp.49-79
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• 2021

피고의 잘못된 임플란트 시술로 인하여 원고는 임플란트 보철물 도재 파절 및 역미소선, 치주염 등의 심각한 손상을 입은 사건에서 피고는 원고에게 불법행위 또는 채무불이행으로 인한 손해배상으로 향후 치료비와 위자료를 지급하라는 판결이 나왔다. 이번 대상판결에서는 치과치료에 관한 사안으로서 일반적인 의료과오소송과 마찬가지로 고도의 전문적 지식을 필요로 하는 분야로서 비전문가인 일반인으로서는 치과의사가 의료행위 과정에서 주의의무 위반이 있었는지 여부나 환자에게 발생한 손해 사이의 인과관계가 있었는지 여부를 밝혀내기 극히 어려우므로 증명책임을 경감하는 것으로 구성한 것이다. 대상판결의 사안에서처럼 수술 도중이나 수술 후에 환자에게 중한 결과의 원인이 된 증상이 발생한 경우에 그 증상의 발생에 관하여 의료상의 과실 이외의 다른 원인이 있다고 보기 어려운 간접사실들이 증명된 경우에는 그와 같은 증상이 의료상의 과실에 기한 것이라고 추정할 수 있다고 판시하였다. 특히 대상판결에서는 일반적인 수술적 치료의 사안이 아닌 임플란트의 시술의 사례로서 수단채무로서 치과진료의 의료과오소송에서 치과의사의 과실에 관한 환자의 입증책임을 소위 '사실상 추정론'에 근거하여 대폭 경감함으로써 의료기술의 발달과 증가하는 현대 의료과오소송에서 세계적 입법추세인 입증책임의 전환에 더욱 가까이 접근하였다는 점에서 큰 의미를 부여할 수 있다. 이러한 점은 대상판결에서 치과의사의 '과실'의 판단에 있어 "그 증상이나 발생에 관하여 의료상의 과실 이외의 다른 원인이 있다고 보기 어려운 간접사실이 증명되면 그와 같은 증상이 의료상의 과실에 기한 것이라고 추정할 수 있다"고 판시한 점에서 명확히 확인된다.

• 한국구조물진단유지관리공학회 논문집
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• 제27권3호
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• pp.30-37
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• 2023

PSCB 거더교는 상하부 플랜지와 복부가 일체화된 폐합된 단면으로 일반적으로 거더와 바닥판이 분리된 교량과 구조적 특성이 상이하여 PSCB 거더교의 특성을 반영한 유지관리 방안이 필요하다. 고속도로 PSCB 거더교의 정밀안전진단 보고서를 수집하여 손상 유형을 분석한 결과, 공용 중 발생되는 열화·손상은 대부분 상부플랜지에 집중되어 있다. 특히 상부플랜지 하면의 교축방향 균열은 분석대상 PSCB 거더교의 약 70 %에서 발생되었고, 이는 외부하중에 의한 구조적 균열 보다 수화열, 건조수축 등 간접하중에 의한 균열로 판단된다. 공용중인 PSCB 거더교의 내구성 향상 및 유지관리 비용 절감 등을 위해서는 설계단계부터 구속 건조수축 균열의 제어가 필요하다. 따라서 본 논문에서는 PSCB 거더교 상부플랜지 하면 균열의 주요 원인인 구속 상태에서의 건조수축으로 인하여 발생되는 균열에 대하여 Gilbert Model을 이용하여 직접 계산하고, 철근량, 철근직경 및 간격 등 영향인자를 분석하였다. 분석결과, 간접하중으로 인하여 발생되는 균열폭은 H16 철근 기준 철근비 0.01 이하, 철근비 0.01 기준 H19보다 직경이 큰 철근의 경우 허용 균열폭 0.2 mm를 초과하는 것으로 나타났으며, 최종적으로 균열폭 검토 결과를 바탕으로 PSCB 거더교 상부플랜지의 균열폭 제어 방안을 제안하였다.

• Journal of Photoscience
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• 제3권1호
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• pp.1-7
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• 1996

When cucumber (Cucumis sativus L.) cotyledon discs were floated on $\delta$-aminolevulinic acid, oxyfluorfen, or rose bengal solution under light condition following 20 h dark incubation, rapid electrolyte leakage from the tissues occurred. The electrolyte leakage from the tissues was dependent on the compounds treated, their concentrations, and the duration of light exposure to the tissues. Dark incubation before exposure to continuous white light enhanced electrolyte leakage from the tissues treated with the compounds and reduced lag period for the activity of the compounds. Electrolyte leakage from the treated tissues was greatly influenced by the light intensity to which they were exposed. Higher light intensities stimulated electrolyte leakage and reduced lag period. Porphyrin biosynthesis inhibitors, gabaculine and 4,6-dioxoheptanoic acid, completely inhibited electrolyte leakage from the oxyfluorfen-treated tissues. Protection against the activity of $\delta$-aminolevulinic acid from electrolyte leakage was complete with 4,6-dioxoheptanoic acid, but not with gabaculine. However, gabaculine and 4,6-dioxoheptanoic acid gave no such protection against rose bengal activity. In summary, our results indicate that $\delta$--aminolevulinic acid, oxyfluorfen, and rose bengal exert their effects by causing electrolyte leakage from the treated tissues in a similar manner, except that oxyfluorfen has an apparent lag period for its action on electrolyte leakage increase. All above compounds require preincubation of treated tissues in darkness and subsequent light exposure with a high intensity for their maximal activities. Our results also support that in the presence of light, $\delta$-aminolevulinic acid and oxyfluorfen cause cellular damage through the indirect generation of singlet oxygen from accumulated tetrapyrroles of porphyrin pathway, whereas rose bengal causes cellular damage through the direct generation of singlet oxygen.

• 한국도로학회논문집
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• 제10권1호
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• pp.75-85
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• 2008

아스팔트 혼합물의 수분손상은 물의 침투로 인해 아스팔트 피막과 골재 사이의 부착력이 손실되면서 발생하는 현상으로서, 이는 아스팔트 포장의 주요 파손형태 중 하나인 포트흘의 주요원인으로 알려져 있다. 이에 본 연구는 반복적인 수침에 의한 수분손상이 일반 아스팔트 혼합물의 재료물성에 미치는 영향을 평가하고자 수행하였다. 이를 위해 기존의 수분손상 시험법인 수정 라트만 시험의 수분 동결-응해 방법을 반복적으로 적용한 일반 아스팔트 혼합물을 대상으로 간접인장시험에 의한 상온물성의 변화특성을 평가하였다. 이를 통해 일반 아스팔트 혼합물의 상온물성은 수침횟수의 증가에 따라 초기에는 급격하게 감소하다가 이후 완만한 감소추세를 나타내었으며, 초기 수분손상에서 물성값의 약 50% 이상이 손실되는 것으로 관측되었다. 또한 파괴시점을 고려한 아스팔트 혼합물의 조기 수분손상 특성을 파악하는데, $25^{\circ}C$의 파괴 에너지와 크리프 변형 에너지를 이용한 재료물성의 손상비가 가장 높은 상관성을 나타내는 것으로 평가되었다.

• 동의생리병리학회지
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• 제16권1호
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• pp.154-159
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• 2002

There are several types of salts that can be classified into raw salt(Chunil salt), purified salts(NaCl reagent grade) and processed salts(Bamboo salt, Purple bamboo salt using in this study) in Korea. Salts has been utilized for the food as well as for the drug in the Oriental Medicine. Purple bamboo salt(PB-salt) was made by heating up to 1300℃ nine times after putting the raw salts in the bamboo. Since salt is generally known to cause the blood pressure to elevate, this study was conducted to investigate the effect of PB-salt on an adverse reaction of the blood pressure elevation. The experiment was performed with the 2 protocols ; 1. Effect of NaCl and PB-salt administration i.v. with the different the concentration(250, 500, 750, 1000mg/kg) on a mean artery blood pressure(MAP) change. 2. Effect of 500mg/kg NaCl and PB-salt administration p.o. for 6 days on the systolic blood pressure change. The results were as follows; The results were as follows; In a short minute change of blood pressure by direct method, PB-salt adminstration did not induced the hypertension unlike NaCl adminstration. In a date change of blood pressure by indirect method, systolic blood pressure was increased about 12-18% in the NaCl administration group, whereas within 6% in the PB-salt administration group from 2 days to 5 days. These results suggest that PB-salt might have some unidentified ingredient to be changed in the course of processing, making it superior to other salts.

• 한국산업보건학회지
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• 제23권3호
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• pp.169-176
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• 2013

Objectives: The aim of this study is to critically review the health effects of not only direct exposure to biocide, but also indirect exposure to by-product hazardous agents generated through the use of biocide in metalworking operations. Methods: An extensive literature review was conducted of studies reporting on respiratory disease cases, particularly hypersensitivity pneumonitis (HP), in environments using water-soluble metalworking fluids (MWFs). Keyword search terms included 'metalworking fluids', 'machining fluids', 'metalworking operation' 'machining operation' and 'biocide', which were also used in combination. Additional articles were identified in references cited in the articles reviewed. Results: Several of the field, epidemiological and experimental studies reviewed assumed that the symptoms and signs typical of HP developed in machinists who handled water-soluble MWF could be caused by inhalation exposure to nontuberculous mycobacteria (NTM). Most NTM are known to be not only resistant to both biocide and disinfectant, but also to have acid-fast cell walls that are highly antigenic. The presence or persistence of the Mycobacterium species, referred to as NTM, in metalworking fluid-using operations may be caused by NTM contamination in either the natural water or tap water that is used to dilute the base oil and additives for water-soluble MWFs. This hypothesis that NTM contamination in water-soluble MWFs is a causative agent of HP has high biologic plausibility, such as antigenic property, hydrophobicity and small diameter (< 5 um). Conclusions: Aerosolized mycobacteria colonized from MWF are likely to be causing the HP. Inhalation exposure to mycobacteria should be considered as a possible cause for the development of HP.