• Textile Coloration and Finishing
• /
• v.27 no.2
• /
• pp.155-163
• /
• 2015

In dyeing cotton fabric with the Indigo Pulverata Levis, this research examined the effect of the sodium dithionite($Na_2S_2O_4$). For the separation of the Indigo-calcium hydroxide complex in the alkaline solution of the Indigo Pulverata Levis, the reduction with the $Na_2S_2O_4$ at $25^{\circ}C$ was more effective than the solution boiling. The concentration of Indigo in the cotton fabric increased with the increase of Indigo Pulverata Levis, but the concentration of indirubin did not increase particularly. The optimum temperature for the reduction was $60^{\circ}C$, and K/S value of dyed fabric decreased at over $60^{\circ}C$. The concentration of indigo and indirubin in the cotton fabric decreased as $Na_2S_2O_4$ concentration increased. In treating cotton fabric dyed with the Indigo Pulverata Levis by the $Na_2S_2O_4$, the concentration of indirubin decreased and the surface color of dyed cotton changed from purple blue to blue while the treatment temperature was getting higher.

• The Journal of Internal Korean Medicine
• /
• v.19 no.2
• /
• pp.261-277
• /
• 1998

The purpose of this research was to investigate cytotoxicity of DangkwiYonghoe-Hwan(DYH) and the constitutive crude drugs on several cancer cell-lines, thymocytes, splenocytes and 3T3 cells. The DYH consists of Coptidis Rhizoma, Scutellariae Radix, Phellodendri Cortex, Gardeniae Fructus, Gentianae scabrae Radix, Indigo pulverata Levis, Aloe, Rhei Rhizoma, Moschus, Saussureae Radix and Angelicae Gigantis Radix. The cytotoxicity was determined by MTT method. The DYH inhibited the proliferation of MOLT-4, K562, HL-60, Jurkat, L1210, P815, S180 and Yac-1, thymocyte, splenocyte and 3T3 cells. The cytotoxicity of Coptidis Rhizoma on the cancer cell-lines was the most potent in the constitutive crude drugs. The proliferation of cancer cell-lines was partly inhibition and partly increase by the treatment of Scutellariae Radix, Gardeniae Fructus, Gentianae scabrae Radix, Indigo pulverata Levis, Aloe, Rhei Rhizoma, Moschus and Angelicae Gigantis Radix. Phellodendri Cortex and Saussureae Radix had a poor cytotoxicity on cancer cell-lines. Coptidis Rhizoma and Phellodendri Cortex inhibited the proliferation of thymocyte, splenocyte and 3T3 cells.

• The Korean Journal of Food And Nutrition
• /
• v.35 no.2
• /
• pp.116-126
• /
• 2022

The purpose of this study was to analyze the anti-inflammatory effect of Chung-Dae Indigo Pulverata Levis, indigo naturalis) produced during indigo dyeing. As a result of in vitro cytotoxicity experiments using RAW 264.7 cell, Chung-Dae extract did not inhibit cell proliferation in Raw 264.7 cells in the range of 1~32 ㎍/mL. NO production was significantly reduced when Chung-Dae extracts were treated at concentrations of 2, 8, and 32 ㎍/mL (p<0.05). The pro-inflammatory cytokines TNF-α, IL-6, IL-1β and IFN-γ significantly decreased when the Chung-Dae extract was treated at concentrations of 2, 8, and 32 ㎍/mL compared to the LPS group, and similarly, the TNFα and IL-6 mRNA levels also decreased. Additionally, the mRNA level of COX-2 was also suppressed. At the protein expression level, the expression of TNF-α, IL-6, iNOS and COX-2 were observed with LPS and Chung-Dae extract significantly decreased compared to the group treated with only LPS (p<0.05). From the above results, it shows that Chung-Dae extract, a plant-derived compound, inhibits the inflammatory response induced by LPS in RAW 264.7 cells. and in particular, regulates the inflammatory response by inhibiting the expression of pro-inflammatory cytokines and inflammation-related enzymes.