• Title/Summary/Keyword: indicator organisms

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Distribution of Indicator Organisms and Incidence of Pathogenic Bacteria in Raw Beef Used for Korean Beef Jerky

  • Kim, Hyoun-Wook;Kim, Hye-Jung;Kim, Cheon-Jei;Paik, Hyun-Dong
    • Food Science and Biotechnology
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    • v.17 no.6
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    • pp.1337-1340
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    • 2008
  • The objective of this study was to evaluate the microbial safety of raw beef used to produce Korean beef jerky, The raw beef samples harbored large populations of microorganisms. In particular, psychrophilic bacteria were found to be most numerous ($9.2{\times}10^3-1.0{\times}10^5\;CFU/g$) in the samples. Mesophilic bacteria and anaerobic bacteria were present in average numbers ($10^3-10^5\;CFU/g$). Spore-forming bacteria and coliforms were not detected below detection limit. Yeast and molds were detected at $2.2{\times}10^1-7.8{\times}10^2\;CFU/g$ in the raw beef. Ten samples of raw beef were analyzed for the presence of pathogenic bacteria. Bacillus cereus was isolated from sample B, G, and H. The B. cereus isolates from raw beef samples were identified with 99.8% agreement according to the API CHB 50 kit.

Relationship between Physical Environmental Factors and Biological Indices of A Mountain Valley Stream (Mt. Cheoggye) (산간계류(청계산)의 물리적 환경요인과 생물지수의 관계)

  • Minjeong Yeo;Dongsoo Kong
    • Journal of Korean Society on Water Environment
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    • v.39 no.4
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    • pp.288-301
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    • 2023
  • This study aims to identify benthic macroinvertebrate fauna inhabiting at the mountain valley stream (Mt. Cheonggye) in Korea and the relationship between physical environmental factors and biological indices. Benthic macroinvertebrates were collected at five locations on August 24 and October 14, 2020, and were identified as 4 phyla, 7 classes, 16 orders, 42 families, and 72 species. Dominance ranged from 0.38 to 0.59, diversity 2.81 to 3.75, richness 3.25 to 4.63, evenness 0.65 to 0.84, and %EPT (Ephemeroptera-PlecopteraTrichoptera) richness value 42% to 73%, respectively. All sites were evaluated as a very good status by mostly biological indices based on tolerance of indicator organisms in Korea. As a result of principal component analysis, biological indices are classified into species-level indices and higher cartegory-level indices according to the taxonomic level of the indicator organism considered in each index. As a result of canonical correspondence analysis, it was confirmed that current velocity was a major factor that increased species richness and classified biological indices according to taxonomic category level. Water depth was a major factor related to the community indices, and the deeper the water depth, the lower the diversity and the evenness.

Effects of Cover Plants on Soil Biota: A Study in an Apple Orchard (사과원에서 피복식물이 토양생물상에 미치는 영향)

  • Eo, Jin-U;Kang, Seok-Beom;Park, Kee-Choon;Han, Kyoung-Suk;Yi, Young-Keun
    • Korean Journal of Environmental Agriculture
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    • v.29 no.3
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    • pp.287-292
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    • 2010
  • We aimed to investigate the responses of soil organisms to cover plants and to provide information for the selection of proper plant species. We studied the effects of 7 cover plants, including rye, oat, rattail fescue, Chinese milk vetch, red clover, crimson clover, and hairy vetch, on soil organisms in an apple orchard. An increase in the microbial phospholipid fatty acids (PLFA) and in the number of nematodes and microarthropods in the soil under the cover plants reflects elevated activities of soil organisms. A decrease in the level of some marker PLFA, which is an indicator of environmental stress, suggests that cover plants provide favorable environments for soil organisms. The population of fungi and animals that feed on fungi increased in the soil surface under red clover. The population density of nematodes and mites increased in the soil surface under rattail fescue, and that of mites and omnivorous nematodes increased in the soil surface under Chinese milk vetch. The level of microbial PLFA in the soil surfaces under the tested cover plants was higher than that under clean culture system. These results suggest that proper selection of the cover plants can facilitate the creation of favorable environments for soil organisms.

Monitoring of Microorganism Contamination in Children-Preferred Confectioneries in Korea (서울·경인지역 유통판매중인 과자류의 위생지표 세균 및 식중독균 오염도 조사)

  • Koo, Eun Joo;Chung, So Young;Park, Ji Eun;Kwon, Yu Jihn;Seo, Dong Hyuk;Jung, Yu Young;Cho, Kyong Chul;Lee, Yo A;Min, Hee Eun;Kim, Eu Gene;Kim, Hyun Jung;Kim, Seul Ki;Choi, Sun Ok;Lim, Chul Ju
    • Journal of Food Hygiene and Safety
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    • v.29 no.4
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    • pp.322-326
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    • 2014
  • According to Centers for Disease Control and Prevention (CDC, USA) recently it was reported that the children (< 5 year-old children) were more susceptible to Foodborne-illness. Confectionery products should be strictly controlled because they are children-preferred foods. Ministry of Food and Drug Safety (MFDS, South of Korea) tried to monitor contamination of organisms in confectionery products (such as biscuits, candies, chewing gums and ice candies) distributed in South Korea. MFDS evaluated the levels of indicator organisms: total aerobic bacteria, coliforms, Escherichia coli as well as the levels of food-borne illness organisms: Staphylococcus aureus, Bacillus cereus, Clostridium perfringens. Experimental plans for microbiological test were in accordance with the International Commission on Microbiological Specifications for Food (ICMSF). For this study, 1,005 samples were collected and from Seoul and Gyeongin region, South Korea. The average level of total aerobic bacteria in 1,005 samples was 1.7 log Colony Forming Unit(CFU)/g and the detection rate was 26.8%. The average level of Bacillus cereus was detected in 1.7 log CFU/g and the rate was 0.9%. There was no detection of coliforms, Escherichia coli, Staphylococcus aureus and Clostridium perfringens. The results of this study will be provided as the basic data to set the reasonable microbiological criteria of Korea Food Code.

Ecosystem Risk Assessment Using the Indicator Species (지표종을 이용한 생태계 위해성평가)

  • Chang, Jin-Soo;Kim, Kyoung-Woong
    • Journal of Soil and Groundwater Environment
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    • v.12 no.1
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    • pp.103-115
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    • 2007
  • Risk assessment by living indicator species provides the information about the ecosystem disturbance, disapperance of symbiosis and change of living group. In the initial stage of this kind research, the degree of contamination was reported using the level of simple number, but simple number may not represent the risk itself which can be casued in the living organisms. Risk assessment using various indicator species overcomes these limitations and can be expanded to the DNA level. In many developed counties, the government has supervised the researches about the indicator species for the monitoring and its application to ecosystem restoration. Several living indicator species found in the vicinity of the abandoned Au mines such as fern, earthworm, bacteria, rhizosphere-rhizoplane, salamamdor and DNA change of these species are described in this paper.

The Development of STEAM Education Textbook on Water Pollution (물의 오염도 측정에 대한 STEAM 교육 교재개발)

  • Hong, Min-Ah;Hwang, Book-Kee;Choi, Jung-Hoon
    • Journal of Environmental Science International
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    • v.21 no.8
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    • pp.909-929
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    • 2012
  • It is an after-school material for elementary school student based on STEAM education which has a theme of water pollution as a global issue. First of all, we take a look at various ways of measuring water pollution and among them, we include experiment for student to know about the water pollution by using indicator organisms. Next, we examine how some elements in our daily life affect on water and provide class with systematically technological and engineering solution. With this text book, we let the student recognize environmental problem and seek for the solution about it. Therefore, it helps student to connect with stream of thought.

Detection and Identification of Bacteriocins Produced by Propionibacteria Isolated from Commercial Swiss Cheese Products

  • Hur, Ji-Woon;Lee, Na-Kyoung;Lee, Haa-Yung;Paik, Hyun-Dong
    • Preventive Nutrition and Food Science
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    • v.2 no.4
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    • pp.310-315
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    • 1997
  • Wild propionibacteria isolated from different commercial swiss cheese samples were tested for antimicrobial activities. In initial screening, six of these Propionibacterium isolates showed antagonistic activity against 10 selected indicator organisms by the deferred method. In next, only two Propionibacterium strains JW6 and JW14 showed antibacterial activity in the cell-free supernatants by the modified well diffusion method. Propionibacterium strains JW6 and JW14 were finally identified as bacteriocin producers which exhibited a bactericidal effect against closely related species. The antimicrobial substances were proteins, since their activities were completely destroyed following several degradative enzyme treatments. The bacteriocins showed a narrow inhibitory spectrum of activity against two propionibacteria and two bacilli of strains tested in this study.

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International Harmonisation of Pesticide Environmental Safety Assessments (농약(農藥) 환경(環境) 안전성(安全性) 평가(評價)의 국제적(國際的)인 조화(調和))

  • Riley, D.;Dyson, J.
    • Korean Journal of Weed Science
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    • v.17 no.1
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    • pp.73-79
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    • 1997
  • Governments and industry have a growing interest in the harmonization of environmental test methods and risk assessment procedures. OECD are currently producing a set of harmonised test guidelines for studying the environmental fate and ecological effects of pesticides. FAO has published an environmental risk assessment procedure. This procedure, which is similar to those used in US and Europe, is based on calculating the ratio of the toxicity of a pesticide to indicator organisms to their level of exposure to the pesticide. The exposure depends on both the concentration of the pesticide and its bioavailability. Ratios which indicate a pesticide will not produce a harmful effect have been established using ecological field studies. Examples are presented for assessing the risk to aquatic ecosystems, earthworms and honeybees. Long-term field studies(up to 20 years) have also shown that pesticides can be used indefinitely without harming soil fertility. Herbicides can be used to avoid the ecologically damaging effects of using soil cultivations excessively for weed control.

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Regulation of Phycocyanin Development by Phenolic Compounds in the Cyanobacterium Anabaena sp. PCC 7120

  • Kim, Jin-Yong;Jo, Yeara;Kim, Young-Saeng;Lee, Eun-Jin;Yoon, Ho-Sung
    • Korean Journal of Ecology and Environment
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    • v.39 no.4 s.118
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    • pp.445-449
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    • 2006
  • Phenolic compounds are manufacturing by-products commonly found in industrial wastewater. The toxicity of high level phenolic compounds in wastewater threatens not only the aquatic organisms, but also many components of the adjacent ecosystem. One of the major light harvesting pigments in cyanobacteria is phycocyanin which can be rapidly and specifically degraded by external stimuli such as nutritional depletion or environmental stress. We employed the cyanobacterium Anabaena sp. PCC 7120 as an indicator organism in estimating the pollution level by phenolic compounds. The phycocyanin content of the cyanobacterium decreased without significantly altering the total chlorophyll as the phenol concentration in a medium increased. We examined the phenol contamination level using the correlation of the phycocyanin content and the phenol concentration. Our results indicated that no significant pollution by phenolic compounds was found in several waterbodies in the vicinity of Daegu, South Korea.

Types and Abundance of Filamentous Bacteria, Protozoa and Metazoa in Activated Sludge (활성오니에서 발견된 사상세균, 원생동물 및 후생동물의 유형과 우점도 분석)

  • 정재춘;김남천
    • Microbiology and Biotechnology Letters
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    • v.23 no.3
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    • pp.365-371
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    • 1995
  • Filamentous bacteria and other large microorganisms, which are useful indicators of bulking and poeration, were identified by microscopic observation. Activated sludge samples were taken from the aeration basin of 5 municipal wasterwater plants and 6 industrial ones. Among the filamentous bacteria founds, Microthrix parvicella was most frequently present, followed by type 0041, type 1701 and Nocardia. This frequency of occurrence was similar to those reorted in USA and the Neterland. The morphology of filamentous bacteria observed were generally identical with those previously reported. except type 1701 which had slender filament diameter. Among protozoa, Vorticella was most frequently present, followed by Aspidisca, Opercularia and Difflugia. Philodina was the only metazoa observed. Both filamentous bacteria and protozoa would be useful indicator organisms. The potential for these organisms as indicators were discussed.

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