• 한국작물학회:학술대회논문집
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• 한국작물학회 2001년도 춘계 학술대회지
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• pp.240-241
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• 2001

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2000년도 제29회 국제학술심포지움 및 추계학술대회
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• pp.24-24
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• 2000

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 추계학술대회
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• pp.153-153
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• 2019

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 춘계학술대회
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• pp.144-144
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• 2019

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.226-226
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• 2022

• Interdisciplinary Bio Central
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• 제2권4호
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• pp.14.1-14.9
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• 2010

Misidentification of cultured cell lines results in the generation of erroneous scientific data. Hence, it is very important to identify and eliminate cell lines with a different origin from that being claimed. Various methods, such as karyotyping and isozyme analysis, can be used to detect inter-species misidentification. However, these methods have proved of little value for identifying intra-species misidentification, and it will only be through the development and application of molecular biological approaches that this will become practical. Recently, the profiling of microsatellite variants has been validated as a means of detecting gene polymorphisms and has proved to be a simple and reliable method for identifying individual cell lines. Currently, the human cell lines provided by cell banks around the world are routinely authenticated by microsatellite polymorphism profiling. Unfortunately, this practice has not been widely adopted for mouse cells lines. Here we show that the profiling of microsatellite variants can be also applied to distinguish the commonly used mouse inbred strains and to determine the strain of origin of cultured cell lines. We found that approximately 4.2% of mouse cell lines have been misidentified; this is a similar rate of misidentification as detected in human cell lines. Although this approach cannot detect intra-strain misidentification, the profiling of microsatellite variants should be routinely carried out for all mouse cell lines to eliminate inter-strain misidentification.

• 농업과학연구
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• 제34권2호
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• pp.131-134
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• 2007

1. 본 시험에 공시된 7개의 찰옥수수 계통중에서 간장은 IK/H26-2, Heunchal, 239-3, IK/H26-1 계통이 대조품종인 Chalok 1호보다 큰 반면에 Local-1, Local-2, IK/LE 계통은 작게 나타났다. 2. 착수고의 경우 Local-2, IK/LE는 각각 23.3cm, 35cm로 대조품종보다 낮았으며, 주당 분얼수는 대조품종인 찰옥1호와 약간의 차이를 보였다. 3. 이삭의 경직경은 IK/H26-1, IK/H26-2, Local-2 239-3 계통 등에서 대조품종보다 낮았고 Local-1, IK/LE, Heunchal 계통 등에서는 다소 굵었다. 4. 출사일수는 Local-1, Local-2, IK/LE 등에서 대조품종보다 2일정도 빨랐고 239-3, Heunchal등에서는 각각 61일, 60일로 다소 늦은 반면에 IK/H26-1, IK/H26-2 등에서는 대조품종과 동일하였다. 특히, 공시 수집종 계통 중에서 Local-2가 55일로 가장 짧은 기간을 나타내어 조숙 찰옥수수 교잡종의 육성 자료로 이용에 유리할 것으로 판단되었다.

• 원예과학기술지
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• 제29권4호
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• pp.336-344
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• 2011

Using the abundant available information about the tomato genome, we developed DNA markers that are linked to disease resistant loci and performed marker-assisted selection (MAS) to construct multi-disease resistant lines and varieties. Resistance markers of Ty-1, T2, and I2, which are linked to disease resistance to Tomato yellow leaf curl virus (TYLCV), Tomato mosaic virus (ToMV), and Fusarium wilt, respectively, were developed in a co-dominant fashion. DNA sequences near the resistance loci of TYLCV, ToMV, and Fusarium wilt were used for primer design. Reported candidate markers for powdery mildew-resistance were screened and the 32.5Cla marker was selected. All four markers (Ty-1, T2, I2, and 32.5Cla) were converted to cleavage amplification polymorphisms (CAPS) markers. Then, the CAPS markers were applied to 96 tomato lines to determine the phenetic relationships among the lines. This information yielded clusters of breeding lines illustrating the distribution of resistant and susceptible characters among lines. These data were utilized further in a MAS program for several generations, and a total of ten varieties and ten inbred lines were constructed. Among four traits, three were introduced to develop varieties and breeding lines through the MAS program; several cultivars possessed up to seven disease resistant traits. These resistant trait-related markers that were developed for the tomato MAS program could be used to select early stage seedlings, saving time and cost, and to construct multi-disease resistant lines and varieties.

• 한국작물학회지
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• 제39권1호
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• pp.103-114
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• 1994

Pearl millet is a $C_4$ plant and summer crop originated from west Africa, and the sixth most important cereal in the world and the most widely cultivated millet in the semi-arid tropics as a major staple food crop. Its grain of higher quality protein is used to make unleavened bread chapatis and prepared as gruel, dumplings, couscous and beer. It is also used as animal feed and forage in both temperate and tropical regions because it has a capability to grow well not only in the fertile soil, but also in the poor and dry soil. Most of the current breeding procedures used in pearl millet are aimed at maximum exploitation of hybrid vigor for both grain and forage yields in Korea. Pearl millet is ideally suited for exploitation of heterosis using cytoplasmic male sterile lines as seed parent, and fertile inbred lines and open-pollinated cultivars as pollen parent. Pearl millet hybrids developed in Korea produced 3 to 7 tons of grain and 100 to 150 tons of green fodder per hectare.

• 응용통계연구
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• 제15권2호
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• pp.367-379
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• 2002

본 연구에서는 부분이면교배에서 p개의 근교계통의 일반조합능력을 비교하기 위한 불완비 블록계획을 구성하는 방법을 제시한다. 부분이면 블록계획은 블록의 크기가 2 이면서 m개의 동반분류를 갖는 부분 균형 불완비 블록계획과균형 불완비 블록계획을 이용하여 구성한다. 또한, p $\leq$ 24일 때 이러한 방법으로 구성되는 블록계획의 효율성을 표로 제시한다.