• 한국식품위생안전성학회지
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• 제23권1호
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• pp.62-67
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• 2008

국내산 과일 주스 4종을 준비하고 식중독 유발균인 Listeria monocytogenes와 Salmonella Typhimurium를 접종하여 14일 동안 $4^{\circ}C$에서 보관하며 항균 효과를 살펴본 결과 다음과 같은 결론을 얻었다. L. monocytogenes 에서는 매실 주스가 가장 뛰어난 효과를 나타내어 보존 14일만에 생존하고 있는 균을 전혀 측정할 수 없었으며, 그 다음으로 키위가 3.0 log만큼 억제를 보이는 항균 효과를 나타내었다. 감귤의 경우 1.0 log만큼 억제하는데 그쳤다. 매실주스 10%를 다른 주스에 혼합하였을 때 L. monocytogenes는 키위 즙에서 더욱 향상된 항균 효과를 나타내어 14일 후 생존 균을 측정할 수 없었고, 감귤주스에서도 1.5 log 만큼의 향상된 항균 효과를 나타내었다. S. Typhimurium은 전반적으로 L. monocytogenes 보다 더 쉽게 억제되는 경향을 보였다. 키위 즙과 매실 주스에서 접종한 모든 균이 보존 14일 후 생존하지 않는 것으로 관찰되었으며, 감귤의 경우에는 1.4 log 만큼밖에 억제하지 못했다. 10%의 매실과 혼합된 주스에 S. Typhimurium을 접종한 결과 키위 즙에서는 접종한 전 균이, 감귤주스에서는 3.2 log 만큼의 증가된 억제효과를 나타내었다. 이상과 같은 결과로 볼 때, 매실은 식중독 유발균에 대한 강한 항균 활성을 가지고 있으며 다른 과실주스에서의 식중독을 예방하기 위한 식품 보존 첨가제로서 사용이 가능할 것으로 기대된다.

• Applied Biological Chemistry
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• 제33권2호
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• pp.109-115
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• 1990

대두 STI의 열처리에 의한 불활성화에 L-cysteine 및 sodium sulfite의 첨가 효과를 조사하고, 대두 품종별 STI활성을 측정하였으며 활성도와 단백질 및 cysteine함량 그러고 소화율과의 관계를 비교하였다. 열처리에 의한 STI의 불활성화에 L-cysteine과 sodium sulfite의 첨가는 불활성화 효과를 크게 증가시켰으며 대두 품종간 불활성화 정도는 차이를 보이지 않았으며 L-cysteine과 sodium sulfite의 첨가는 불활성화된 STI의 재활생화를 크게 억제하였다. 품종별 STI의 활성도는 대두분말 g당 $64.7{\sim}86.47\;TIU$의 범위에 있었으며, 장백>힐>장엽, 광교>단엽>백운>단경>팔달, 새알, 덕유>황금 이었으며, 단백질 함량과 STI의 활성도와는 상관관계(r=-0.192)가 없는 것으로 나타났다. 품종별 cysteine 함량은 힐, 장백, 단경, 단엽, 황금, 백운, 장엽, 새알, 덕유, 광교, 활달의 순서이었으며, cysteine의 함량은 $73.5{\sim}40.0\;{\mu}mole/g$ 대두분 이었다. 또한 cysteine함량과 STI 활성도 사이에 정의 상관(r=0.6568)을 나타냈다. 품종별 소화율은 광교, 백운, 팔달, 단경, 새알, 힐, 장엽, 덕유, 황금, 장백, 단엽의 순서이었으며 $81.9{\sim}76.7%$ 정도이었다. 또한 소화율과 STI활성도 사이에는 부의 상관(r=-0.7695)을 나타내었다.

• 한국연초학회지
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• 제13권2호
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• pp.27-33
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• 1991

Current studies indicated that emphysema in smokers might be due, in part, to the local suppression of G, -protease inhibitor(u, -Pl) in lung by reactive oxygen species in cigarette smoke or smoke-activated lung neutrophiles. In the present works, we examined the possibility that a measure which inactivated $\alpha$l-Pl by cigarette smoke could be an alternative method to evaluate the cigarette quality, In order to determine the inactivation of $\alpha$1, -Pl, trypsin inhibitory capacity(TIC) was assayed. A rapid loss of $\alpha$1, -Pl activity occurred when $\alpha$1-Pl solutions was exposed the gas phase or total particulate matter(TPM) obtained from various brands. The inactivation of $\alpha$1-Pl by gas phase was dependent upon the number of puffs and the age of the smoke. However, that by TPM was rather decreased since 2 puffs and also showed no more change over 24hrs after exposing. Inactivation of $\alpha$1-Pl determined by our suggested method(5 puffs, 24hours of aging after exposing) using various commercial cigarettes exhibited that high tar brands has inactivated it more strongly than low tar cigarettes. But the ability of some brands to inactivate $\alpha$1-Pl does not correlate with the content of tar or nicotine. These results so여esc that the degree of $\alpha$1-Pl inactivation by cigarette smoke may be a useful index for the evaluation of cigarette quality and that it should be also contribute to the manufacture of less hazardous cigarettes.

• 한국농공학회:학술대회논문집
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• 한국농공학회 2002년도 학술발표회 발표논문집
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• pp.421-424
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• 2002

Deterministic and probabilistic approaches to the design of ultraviolet (UV) disinfection system for water reclamation are reviewed and discussed. The high inactivation of TC, FC and E. coli by UV disinfection was demonstrated and the inactivations of TC, FC and E. coli were 97%, 98% and 99%, respectively. Within the range of 0.3-4.5mWs/cm, the effect of UV does on the inactivation ratio was not observed. However, in the highest wattage of UV lamp, 39W, the inactivation ratio of TC, FC and E. coli was 100%, regardless of the UV does so the UV density was more effective on inactivation ratio of TC, FC and E. coli rather than UV does. Under the 0.4 mWs/cm and 16W of UV lamp, the effect of dissolved organic matter and turbidity on the inactivations of TC, FC and E. coli could not be observed in this study within the range of 0-60mg/L and 0-40 NTU respectively.

• 한국미생물·생명공학회지
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• 제26권5호
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• pp.387-392
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• 1998

B. lactofermentum의 lysine 생합성에 있어서 DDH경로 및 ddh gene이 지닌 중요성을 조사하기 위하여, site-specific mutagenesis technique를 통하여 B. lactofermentum의 ddh gene을 disruption함으로서 DDH 경로를 차단시켰다. B. lactofermentum ddh mutant는 wild type 및 AEC내성 균주보다 성장이 매우 저조하였으며 lysine 생산량에서도 급격한 저하를 가져왔다. 이와 같이 B. lactofermentum이 DAP 경로만을 가졌을 때 세포의 성장 및 lysine 생산량에 있어서 극적인 저하를 가져왔기 때문에 B. lactofermentum에서의 DDH 경로는 meso-DAP 및 lysine 생합성에 있어 필수적인 경로로 작용한다는 것을 확인하였다. 그러므로 C. glutamicum과 B. lactofermentum과 같은 corynebacteria가 lysine을 많이 생산하는 것은 DDH 경로가 부가적으로 존재하기 때문이며, 이러한 DDH 경로는 metabolic flux가 증가되면 중간 대사물을 lysine으로 변화시키는 중요한 경로로 작용할 것이라 사료된다.

• Environmental Engineering Research
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• 제21권4호
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• pp.350-354
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• 2016

There is an urgent need to identify more reliable indicator systems for human pathogenic viruses in water reuse practice. In this study, we determined the response of different bacteriophages representing various bacteriophage groups to different ultraviolet (UV) technologies in real wastewater in order to identify more reliable bacteriophage indicator systems for UV disinfection in wastewater. Bacteriophage ${\varphi}X174$ PRD1, and MS2 in two different real wastewaters were irradiated with several doses of both low pressure (LP) and medium pressure (MP) UV irradiation through bench-scale UV collimated apparatus. The inactivation rate of ${\varphi}X174$ by both LP and MP UV was rapid and reached ${\sim}4{\log}_{10}$ within a UV dose of $20mJ/cm^2$. However, the inactivation rates of bacteriophage PRD1 and MS2 were much slower than the one for ${\varphi}X174$ and only ${\sim}1{\log}_{10}$ inactivation was achieved by the same UV dose of $20mJ/cm^2$. Overall, the results of this study suggest that bacteriophage MS2 could be a reliable indicator for human pathogenic viruses for both LP and MP UV disinfection in wastewater treatment processes and water reuse practice.

• Applied Biological Chemistry
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• 제22권1호
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• pp.24-27
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• 1979

Horseradish peroxidase의 열불활성화에 미치는 몇가지 인자의 영향에 대해 실험하였다. lecithin이 존재하는 용액 속에서 peroxidase를 pH 7.0 $70^{\circ}C$에서 처리한 결과 급격한 열불활성화를 보였고 pH 4.0의 용액에서는 $0^{\circ}C$에 짧은 시간 방했을 때도 급격한 불활성화를 나타내었다. linoleic acid는 산소 존재하에서 처리 했을 때가 질소 존재 하의 처리에서 보다 큰 영향을 보였고 oleic acid의 경우는 산소및 질소 존재 하에서 비슷한 영향을 보였다. lecithin과 linoleic acid의 영향에 대한 결과 각각 heme crevis의 개열 내지는 생성된 lipoperoxide에 의한 불활성화라고 추정하였다.

• 한국입자에어로졸학회지
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• 제12권3호
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• pp.73-80
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• 2016

Bioaerosol inactivation becomes important as people recognize the significance on the health effects of bioaerosols. There are several ways to inactivate such bioaerosols such as antimicrobial filters, UV, etc. For the on-filter-inactivation, proper antimicrobial materials coating should be applied. Recently, air ions are adopted to effectively reduce germ and virus activity. Limitations arise when each method is applied separately. Coating materials can experience chemical instability over time and temperature. Ionizers can generate ozone to prepare high ion concentrations. Combinations of developed techniques to enhance the inactivation efficiency were suggested. Researches on the air sterilization are reviewed and outlook is highlighted. Proper techniques such as combinations of filter material coating and air ion generation can be used to make air quality better for human living.

• Archives of Pharmacal Research
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• 제17권4호
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• pp.218-222
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• 1994

The natural product, spirostanol glycoside dioscin, was shown to directly inactivate human pleural fluid phospholipase $A_2{\;}(PLA_2)$ Inactivation was dose, and time dependent. The $IC_{50}$ was estimated at 18 .mu.M and virtually complete inactivation of the enzyme occurred at 50 .mu.M. Using Michaelis-Menten kinetics, dioscin inactivated the enzyme by a competitive inhibitory manner, the apparent Ki value was $6.9{\times}10_{-4}$. Reversibility was studied directly by dialysis method, the inhibition was reversible. Additioin of excess $Ca^{2+}$ concentration up to 8 mM did not antagonize the inhibitory activity of dioscin. Inactivation of several kinds of $PLA_2$ by dioscin is due to interaction with the active site of $PLA_2$ and may be a useful adjunt in the theraphy of inflammatory diseases.

• BMB Reports
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• 제29권4호
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• pp.321-326
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• 1996

Spinach glycolate oxidase was subjected to a series of chemical modifications aimed at identifying amino acid residues essential for catalytic activity. The oxidase was reversibly inactivated by treatment with pyridoxal 5'-phosphate (PLP). The inactivation by PLP was accompanied by the appearance of an absorption peak of around 430 nm, which was shifted to 325 nm upon reduction with $NaBH_4$. After reduction, the PLP-treated oxidase showed a fluorescence spectrum with a maximum of around 395 nm by exciting at 325 nm. The substrate-competitive inhibitors oxalate and oxaloacetate provided protection against inactivation of the oxidase by PLP. These results suggest that PLP inactivates the enzyme by fonning a Schiff base with lysyl residue(s) at an active site of the oxidase. The enzyme was also inactivated by tryptophan-specific reagent N-bromosuccinimide (NBS). However, competitive inhibitors oxalate and oxaloacetate could not protect the oxidase significantly against inactivation of the enzyme by NBS. The results implicate that the inactivation of the oxidase by NBS is not directly related to modification of the tryptophanyl residue at an active site of the enzyme. Treatments of the oxidase with cysteine-specific reagents iodoacetate, silver nitrate, and 5,5'-dithiobis-2-nitrobenzoic acid did not affect significantly the activity of the enzyme.