• 대한수의학회지
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• 제43권3호
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• pp.477-483
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• 2003

Molecular diagnostic techniques have been used to identify porcine epidemic diarrhea virus (PEDV), a causative agent of acute enteritis in swine, but they were difficult to be petformed and time-consuming. To detect PEDV in a rapid and easy way, we developed biotinylated cDNA probe for N gene encoding the nucleoproteins of PEDV. Formalin-fixed and paraffin-embedded tissues from 24 naturally infected pigs were used for the experiment. The ISH produced a positive reaction in all cases. When intestinal tissues were hybridized with PEDV probe, strong signals were seen in the villus enterocytes of the jejunum and ileum. Hybridization signals were also found in the duodenum from one pig and in colon from dnother. In conclusion, ISH with a biotinylated cDNA probe was provided to be a useful diagnostic method for detecting PEDV effectively in routinely processed tissue sections.

• Korean Chemical Engineering Research
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• 제47권3호
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• pp.287-291
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• 2009

전도된 정체점 흐름을 갖는 반응기 안의 가열된 석영(quartz) 표면에서 암모니아($NH_3$)가 분해되는 반응을 실험과 수치 모사을 이용하여 조사하였다. 질소($N_2$)와 혼합된 8 mole%의 암모니아를 사용하였고 반응 표면으로 사용된 석영 표면을 가열하기 위한 전열기의 온도는 $300{\sim}900^{\circ}C$ 범위로 설정하였다. 라만 분광법(in situ Raman spectroscopy)을 이용하여 획득한 반응기 내부의 온도와 암모니아 농도 정보를 반응기 모델을 이용하여 분석한 결과, 전열기의 온도 설정에 의존하는 석영 표면의 온도는 $235{\sim}619^{\circ}C$ 범위였으며 암모니아 분해 반응의 활성화 에너지는 10.9~15.8 kcal/mol 범위를 가졌다.

• 환경생물
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• 제18권1호
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• pp.53-61
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• 2000

서울시 소재 지하수 중 중금속으로 오염되어 음용수 이외의 생활 용수로 사용하고 있는 1개 정점과 음용수로 사용하고 있는 1개 정점, 대조군으로 강화도 천연 동굴의 1개 정점을 대상으로 실험을 실시하였다. 지하수 세균군집의 유전적 다양성의 변화를 보기 위해 지하수 세균 군집에서 16SrDNA를 증폭하는 primer로 PCR(polymerase chain reaction)을 실시한 후 ARDRA(amplified ribosomal DNA restriction analysis) 지문 분석으로 비교하였다. 16S rDNA를 증폭하여 제한효소 지문분석을 한 결과 in situ와 음용수에서 유전적 다양성이 상대적으로 크게 나타났다. 지하수 세균 군집의 ARDRA지문 분석은 상이한 환경과 서식지를 반영한 유전적 차이를 빠르게 비교 분석할 수 있었으며 지하수의 오염도에 따른 미생물의 다양성(천연 동굴>음용수>오염수)을 확인할 수 있었다.

• Fisheries and Aquatic Sciences
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• 제9권4호
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• pp.146-152
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• 2006

Systemic infections of maricultured fishes by Megalocytivirus species have occurred over a broad area in South Korea, causing extensive economic loss. We developed digoxigenin-labeled nucleic acid probes against the 230-bp ATPase and 311-bp major capsid protein (MCP) of rock bream Oplegnathus fasciatus iridovirus (RBIV) using polymerase chain reaction, and an in situ hybridization (ISH) method to detect Megalocytivirus in formalin-fixed tissues of mariculture species (rock bream, sea bass, and olive flounder). ISH-positive cells were abundant in the hematopoietic and connective tissues of various organs, while brain tissue showed little or no signal. The ISH procedure can become an important diagnostic tool in complement with histopathological methods, and advances epidemiological studies on the origin and distribution of Megalocytivirus in mariculture.

• 한국초전도ㆍ저온공학회논문지
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• 제16권1호
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• pp.19-22
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• 2014

The effects of Mg content on the pore formation, density and critical properties were investigated in in-situ reacted $MgB_2$ superconductors. The $Mg_{1+x}B_2$, (x=-0.2, 0.0, 0.05, 0.3, 1.0) bulk samples with different Mg contents were heat-treated at $900^{\circ}C$ for 1 h in an Ar atmosphere. The dimensional changes of a pellet's mass and volume after heat-treatment were measured. After heat-treatment process, the sample mass was decreased by Mg evaporation, but the sample volume was expanded by pore formation at the Mg site; therefore, the apparent density was decreased. Spherical pores the same as Mg particles were developed after heat-treatment in all samples, and the pore density was increased with increasing Mg content. As the x of Mg content was increased to 1.0, the apparent density of $Mg_{1+x}B_2$ samples was decreased due to a relatively larger reduction in a mass change. The critical current density of Mg excessive sample of x=0.05 showed the highest values over the applied magnetic fields because the excessive Mg may compensate Mg loss and enhance grain connectivity.

• Macromolecular Research
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• 제12권1호
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• pp.107-111
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• 2004

We have studied the polymerization kinetics of acrylamide in aqueous solution with potassium persulfate as an initiator by using quantitative real-time in situ IR spectroscopy and monitoring the profiles of peaks in the range 1900-850 cm$\^$-1/. The conversion of acrylamide was calculated from the disappearance of the peak at 988 cm$\^$-1/, which is the out-of-plane bending mode of the=C-H unit, normalized to the C=O stretching peak at 1675 cm$\^$-1/, as an internal standard. For reaction temperatures in the range 40-65$^{\circ}C$ and initiator and monomer concentrations of 0.9-2.6 mmol/L and 0.5-1.1 mol/L, respectively, we deduced that the rate of monomer consumption follows the relation R$\_$p/=k［K$_2$S$_2$O$\_$8/］$\^$0.5/ [Μ］$\^$1.35${\pm}$0.10/. In addition, we obtained activation parameters from an evaluation of the kinetic data.

• 한국미생물·생명공학회지
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• 제18권5호
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• pp.525-529
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• 1990

식물세포인 Lithospermum erythrorhizon을 교반 반응기와 calcium alghinate에 고정화된 상태로 충전층 반응기에서 n-hexadecane으로 동시 추출하면서 shikonin을 생산하여 각각의 생산성을 비교하였다. 교반 반응기에서 shikonin의 비생산과 부피생산성은 각각 1.5mg shikonin/g cell과 400$\mu g$ shikonin/(L.day)였고 충전층 반응기에서는 가각 2.0mg shikonin/g cell과 2857 $\mu g$ shikonin/(L.day)였으며 이는 각각 교반 반응기에 비하여 1.3, 7.1배 높은 것이다. 충전층 반응기에서 shikonin의 생산성이 높은 것은 calcium alginate 입자에 세포가 고농도고 축적되어 단위 반응기 부피당 세로의 부하 능력이 높고 또한 세포가 서로 접촉하기가 쉽고 고정화 입자내의 환경이 세포가 분화하기에 좋은 조건을 형성하기 때문인 것으로 사료된다.

• Composites Research
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• 제33권5호
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• pp.256-261
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• 2020

본 연구에서는 Al-Ti-C 반응계의 점화온도에 대해 고찰하고, 자전연소합성법 및 교반주조 공정을 통해 TiC/Mg 금속복합재료를 제조하여 미세조직 및 기계적 특성을 분석하였다. 0, 10, 20, 30 vol.% TiC 입자가 균일하게 분산된 Mg 복합재료를 제조하였고, 강화재의 양이 증가할수록 기지 대비 우수한 압축강도 및 내마모특성을 보였다. 이는 in-situ 자전연소합성법에 의해 결함이나 불순물 등의 오염이 적은 TiC/Mg 금속복합재료 제조로 기지에서 강화재로의 효과적인 하중 전달에 의한 것으로 판단된다.

• 한국재료학회지
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• 제17권6호
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• pp.313-317
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• 2007

In-situ micro-channeled multi tubular solid oxide fuel cell(SOFC) was fabricated using multi-pass extrusion process with out side diameter of 2.7 mm and active length of 5 mm that contained 61 individual cells. Cell materials used in this work were NiO-YSZ (50 : 50 vol.%), 8 mol% yttria-stabilized zirconia(8YSZ), $La_{0.8}Sr_{0.2}MnO_3(LSM)$ as anode, electrolyte, and cathode, respectively. The arrangement of each electrode and electrolyte layer in green bodies showed uniformity and integrity after extrusion and sintering. The XRD analysis confirmed that no reaction phases appeared and the microstructure of the electrolyte was fairly dense (relative density > 96%) after sintering.

• Bulletin of the Korean Chemical Society
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• 제34권8호
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• pp.2343-2347
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• 2013

In this work, manganese dioxide ($Mn_3O_4$)/carbon black (CB) composites (Mn-CBs) were prepared by an in situ coating method as electrical fillers and the effect of the Mn-CBs on the electrical performance of activated carbon (AC)-based electrodes was investigated. Structural features of Mn-CBs produced via in situ coating using a $KMnO_4$ solution were confirmed by XRD and TEM images. The electrical performances, including cv curves, charge-discharge behaviors, and specific capacitance of the ACs/Mn-CBs, were determined by cyclic voltammograms. It was found that the composites of $Mn_3O_4$ and CBs were successfully formed by in situ coating method. ACs/Mn-CBs showed higher electrical performance than that of AC electrodes fabricated with conventional CBs due to the pesudocapacitance reaction of manganese oxides in the aqueous electrolyte. Consequently, it is anticipated that the incorporation of $Mn_3O_4$ into CBs could facilitate the utilization of CBs as electrical filler, leading to enhanced electrochemical performance of AC electrodes for supercapacitors.