• 한국축산식품학회지
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• 제33권4호
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• pp.522-530
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• 2013

Probiotic 기능성이 우수한 유산균을 선발하기 위하여 9개월 미만의 모유수유아로부터 100종의 Lactobacillus균을 분리하였다. C. elegans를 이용한 in vivo 실험으로 장내 우점능을 검사하여 L. rhamnosus GG와 비교했을 때 다른 균주들보다 상대적으로 장환경에 대하여 우수한 우점특성을 갖는 probiotic 유산균주 9종을 선발하였다. Mucin을 이용한 in vitro 부착능력 검토결과, L. rhamnosus GG대비 90% 이상의 부착능을 보였으며, pH 2.5에서의 내산성 및 0.5% oxgall을 함유한 내담즙성에서 각각 97% 이상과 99% 이상의 높은 생존율을 나타냈다. 또, 81% 이상의 높은 콜레스테롤 저해능과 다양한 prebiotics 이용가능성을 확인하였고 최종적으로 L. rhamnosus 4종, L. plantarum 5종인 것으로 동정되었다. 이상의 결과로부터 in vivo 실험을 통해 우선 선발한 균주들이 프로바이오틱스 균주로서 요구되는 조건을 우수하게 충족시킨다는 것을 알 수 있었으며, 따라서 in vivo 모델로서 C. elegans을 이용한 장내우점능력 검토는 내산성, 내담즙성, 콜레스테롤 저하, 그리고 prebiotic 기질 이용능 등의 고기능성의 probiotic 균주 선발에 직접적으로 이용할 수 있을 것으로 기대된다.

• 식물병연구
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• 제15권2호
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• pp.101-105
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• 2009

Serratia plymuthica A21-4는 양파(Allitum fistulosum L.) 근권에서 분리된 고추 역병 생물적 방제 미생물이다. 토양 환경이 S. plymuthica A2l-4 근권정착에 미치는 영향을 알아본 결과 사질이 많이 포함되어 공극이 많은토양에 3% 중량(w/w)의 부숙된 옥수수 줄기를 첨가하고 토양 수분함양이 40% 정도, 토양온도가 $20^{\circ}C$, 토양 pH가 중성이거나 약산성 토양이 S. plymuthica A2l-4의 근권정착에 유리하였다. 그리고 토착미생물이 있는 것이 살균한 토양에서 보다 S. plymuthica A21-4의 근권정착에 유리하였다.

• 대한한의학회지
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• 제22권2호
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• pp.22-30
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• 2001

This study was attempted to investigate the anti-tumor and anti-metastatic effects of Sobokchukeotang(SBCT) and Kamisobokchukeotang(KSBCT). Cytotoxicity against various cancer cell lines, anti-adhesion, pulmonary colonization, anti-angiogenesis, and T/C% were evaluated. SBCT and KSBCT exhibited no cytotoxicity against HT-1080, A549, SK-OV-3, B16-F10 and SK-Mel-2 cell lines. In inhibitory effect on DNA topoisomerase I, the $IC_{50S}$ were shown $250-500{\;}\mu\textrm{g}/ml$ of SBCT and $62.5-125{\;}\mu\textrm{g}/ml$ of KSBCT respectively. In the in vivo experiments, SBCT(135.98%) and KSBCT(151.92%) apparently increased the life span of mice bearing sarcoma-180. KSBCT significantly inhibited the adhesion of HT-1080 to complex extracellular matrix in a dose-dependent manner in contrast to SBCT. In pulmonary colonization assay by B16-F10, a number of colonies in the lungs were decreased more significantly in KSBCT group than those in SBCT group. In vitro neovascularization and CAM assay, angiogenesis was more significantly inhibited in KSBCT-treated group than in SBCT- treated group. Above results suggests that KSBCT is more effectively applied to prevention and treatment of cancer than SBCT.

• Preventive Nutrition and Food Science
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• 제21권3호
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• pp.197-201
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• 2016

Helicobacter pylori infection is associated with an increased risk of developing upper gastrointestinal tract diseases. However, treatment failure is a major cause of concern mainly due to possible recurrence of infection, the side effects, and resistance to antibiotics. The aim of this study was to investigate the activities of Centella asiatica leaf extract (CAE) against H. pylori both in vitro and in vivo. The minimum inhibitory concentrations (MICs) against 55 clinically isolated strains of H. pylori were tested using an agar dilution method. The MICs of CAE ranged from 0.125 mg/mL to 8 mg/mL, effectiveness in inhibiting H. pylori growth was 2 mg/mL. The anti-H. pylori effects of CAE in vivo were also examined in H. pylori-infected C57BL/6 mice. CAE was orally administrated once daily for 3 weeks at doses of 50 mg/kg and 250 mg/kg. CAE at the 50 mg/kg dose significantly reduced H. pylori colonization in mice gastric mucosa. Our study provides novel insights into the therapeutic effects of CAE against H. pylori infection, and it suggests that CAE may be useful as an alternative therapy.

• Journal of Periodontal and Implant Science
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• 제49권1호
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• pp.58-58
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• 2019

• The Journal of Advanced Prosthodontics
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• 제4권3호
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• pp.134-138
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• 2012

PURPOSE. The study was conducted to evaluate the efficacy of implant supported tooth replacement in diabetic patients. MATERIALS AND METHODS. The study involved placement of implants (UNITI implants, Equinox Medical Technologies, Zeist, Holland, diameter of 3.7 mm and length 13 mm) in five diabetic patients (three females and two males) of age ranging from 35-65 years with acceptable metabolic control of plasma glucose. All patients included in the study were indicated for single tooth maxillary central incisor replacement, with the adjacent teeth intact. The survival of the restored implants was assessed for a period of three months by measurement of crestal bone heights, bleeding on probing and micro flora predominance. Paired t-test was done to find out the difference in the microbial colonization, bleeding on probing and crestal bone loss. P values of less than 0.05 were taken to indicate statistical significance. RESULTS. Results indicated that there was a significant reduction in bleeding on probing and colonization at the end of three months and the bone loss was not statistically significant. CONCLUSION. The study explores the hypothesis that patients with diabetes are appropriate candidates for implants and justifies the continued evaluation of the impact of diabetes on implant success and complications.

• 대한한의학회지
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• 제28권4호
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• pp.27-41
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• 2007

Aim : This study aimed at elucidating the effects of Inonotus obliquus on anti-tumor effects in vivo and immune-based characterization of the mushroom as a potential candidate for cancer remedy. Methods : To investigate the immunomodulatory effects of Inonotus obliquus, we investigated macrophage functions and NK cell activities through the measurement of NO production of macrophage, NK cell cytotoxicity and expressions of cytokines and genes regulating immune responses, in addition to pulmonary metastasis model in vivo. Results : Inonotus obliquus showed general cytotoxicity at high concentrations over the 100 ${\mu}g/m{\ell}$ on the both of normal and cancer cell lines. Inonotus obliquus showed both inhibitory and promotive effects on pulmonary colonization of CT-26 cell depending on period or route of administration in vivo. Conclusion : From these results, it cannot be concluded that Inonotus obliquus has cancer-specific activity. Furthermore, Inonotus obliquus has the provability to show adverse effects differently according to the concentration and the method of administration.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.59-62
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• 2002

The role of a TolC homologue in the virulence of Vibrio vulnificus, a marine bacterium causing serious wound infection and fulminant septicemia in persons with underlying conditions, has been studied. TolC, an outer membrane protein, has been implicated in a variety of bacterial functions including export of diverse molecules ranging from large proteins to antibiotics. A homologue of the tolC gene of V. cholerae, which has been shown to be required for bile resistance, cytotoxicity and colonization of this organism, was identified in the partially determined genome sequence of V. vulnificus. To determine the role of TolC in the virulence of V. vulnificus, a TolC-deficient (TD) mutant was isolated by in vivo allelic exchange. Compared with the parent strain, the TD mutant was more sensitive to bile, and much less virulent in mice challenged subcutaneously. This mutant was noncytotoxic to the HEp-2 cells, but its metalloprotease and cytolysin activities in the culture supernatant were comparable to the parent strain. In addition, the resistance of the TD mutant to human serum bactericidal activity as well as its growth in either human or murine blood was not affected. Collectively, our data suggest that TolC may be involved in colonization and/or spread of V. vulnificus to the blood stream, probably by secreting a cytotoxin other than the cytolysin.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1383-1392
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• 2021

Carbapenem-resistant Enterobacteriaceae (CRE) that produce Klebsiella pneumoniae carbapenemase are increasingly reported worldwide and have become more and more resistant to nearly all antibiotics during the past decade. The emergence of K. pneumoniae strains with decreased susceptibility to carbapenems, which are used as a last resort treatment option, is a significant threat to hospitalized patients worldwide as K. pneumoniae infection is responsible for a high mortality rate in the elderly and immunodeficient individuals. This study used Lactobacillus fermentum as a candidate probiotic for treating CRE-related infections and investigated its effectiveness. We treated mice with L. fermentum originating from the vaginal fluid of a healthy Korean woman and evaluated the Lactobacilli's efficacy in preventive, treatment, nonestablishment, and colonization mouse model experiments. Compared to the control, pre-treatment with L. fermentum significantly reduced body weight loss in the mouse models, and all mice survived until the end of the study. The oral administration of L. fermentum after carbapenem-resistant Klebsiella (CRK) infection decreased mortality and illness severity during a 2-week observation period and showed that it affects other strains of CRK bacteria. Also, the number of Klebsiella bacteria was decreased to below 5.5 log₁₀ CFU/ml following oral administration of L. fermentum in the colonization model. These findings demonstrate L. fermentum's antibacterial activity and its potential to treat CRE infection in the future.

• Biomolecules & Therapeutics
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• 제23권1호
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• pp.53-59
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• 2015

In this study, we investigated the inhibitory activities on gastritis and gastric ulcer using liriodendrin which is a constituent isolated from Kalopanax pictus. To elucidate its abilities to prevent gastric injury, we measured the quantity of prostaglandin $E_2$ ($PGE_2$) as the protective factor, and we assessed inhibition of activities related to excessive gastric acid be notorious for aggressive factor and inhibition of Helicobacter pylori (H. pylori) colonization known as a cause of chronic gastritis, gastric ulcer, and gastric cancer. Liriodendrin exhibited higher $PGE_2$ level than rebamipide used as a positive control group at the dose of $500{\mu}M$. It was also exhibited acid-neutralizing capacity (10.3%) and $H^+/K^+$-ATPase inhibition of 42.6% ($500{\mu}M$). In pylorus-ligated rats, liriodendrin showed lower volume of gastric juice ($4.38{\pm}2.14ml$), slightly higher pH ($1.53{\pm}0.41$), and smaller total acid output ($0.47{\pm}0.3mEq/4hrs$) than the control group. Furthermore liriodendrin inhibited colonization of H. pylori effectively. In vivo test, liriodendrin significantly inhibited both of HCl/EtOH-induced gastritis (46.9 %) and indomethacin-induced gastric ulcer (46.1%). From these results, we suggest that liriodendrin could be utilized for the treatment and/or protection of gastritis and gastric ulcer.