• Title/Summary/Keyword: in vitro protein digestibility

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Studies on the Quality and Productivity Improvement by Mixed Sowing of Oat-Hairy Vetch (연맥-헤어리베치 혼파에 의한 사료가치 및 생산성 향상 연구)

  • Kim, J.G.;Chung, E.S.;Yoon, S.H.;Seo, S.;Seo, J.H.;Park, G.J.;Kim, C.K.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.1
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    • pp.31-36
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    • 2002
  • The yield and quality of winter forage crops such as oats could increase by mixed sowing with a winter legume such as hairy vetch(Vicia villosa Roth). The purpose of this study was to improve the forage quality and productivity using Oat-Hairy vetch mixture in winter season for three years. The results of this experiments were summarized as follows. In mix seeding of oat and hairy vetch, the dry matter(DM) content of herbage was 15∼16% in fall-harvest and 19∼20% in spring-harvest. Crude protein(CP) content of hairy vetch was the highest as 30.1% and the mixture was higher than that of oat. Acid detergent fiber(ADF) and neutral detergent fiber(NDF) content of hairy vetch was lower, but in vitro dry matter digestibility(IVDMD) and total dry matter digestibility(TDN) content was higher than that of oat. Although oat showed the low yield by 3.7∼4.4 DM t/ha, the Fall-seeded-Spring-harvest hairy vetch plot showed highly production by 9.5 DM t/ha. Crude protein and TDN yield of Fall-seeded-Spring-harvest plots were higher than those of others. According to this study, the highest dry matter yield could be obtained by Fall-seeded-Spring-harvest. It would be recommend in cropping field for oat.

Use of N-alkanes to Estimate Intake and Digestibility by Beef Steers

  • Premaratne, S.;Fontenot, J.P.;Shanklin, R.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.11
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    • pp.1564-1568
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    • 2005
  • The objective of the study was to evaluate the use of n-alkanes to estimate DM intake and digestibility by beef cattle. Six steers were blocked (3 blocks, 2 animals/block) according to the body weight (279${\pm}$19 kg) and randomly allotted within blocks to two diets (3 steers/diet). A second trial was conducted with the same animals (321${\pm}$18 kg) after 36 days (d), using a switch back design. The diets consisted of two types of chopped sun-cured hay, alfalfa (Medicago sativa L) hay, or fescue (Festuca arundinacea Schreb) and alfalfa mixture, which were fed in equal amounts to steers. Animals were dosed with $C_{32}$ and $C_{36}$ alkanes, employing an intra-ruminal controlled-release device at the beginning of each trial. Hay intake per animal was measured from d 6 to 12 and sub samples were taken for chemical analysis. Rectal samples of feces were taken from each animal once/daily from d 8 to 14, freeze dried, and ground prior to alkane analysis. Alkanes were extracted from ground hay and feces. Feed intake was calculated from the dose rate of $C_{32}$ alkane and, the herbage and fecal concentrations of adjacent odd ($C_{33}$ or $C_{31}$) and even ($C_{32}$) chain length alkanes. Crude Protein, NDF, ADF, ash concentrations and In vitro dry matter digestibility (IVDMD) were 17.7, 42.2, 28.4, 7.9 and 71.7 for alfalfa, and 12.4, 56.5, 30.4, 6.9 and 69.1% for fescue/alfalfa mixture, respectively. For both diets, intake estimated from $C_{33}$:$C_{32}$ ratio was not different from the measured intake, but intake estimated from $C_{31}$:$C_{32}$ ratio was lower (p<0.05), than the measured intake for both diets. The average estimated forage intake from $C_{33}$:$C_{32}$ ratio was 4.86 and 0.69% below than the measured intake for alfalfa and, fescue/alfalfa mixed diets, respectively. The respective estimates with $C_{31}$:$C_{32}$ ratio were 9.59 and 11.33% below than the measured intake. According to these results, alkane $C_{33}$:$C_{32}$ ratio is better than alkane $C_{31}$:$C_{32}$ ratio for the estimation of intake by beef steers.

Analysis of Ruminal Dry Matter and Crude Protein Digestibility on Major Roughage, Wormwood and Green Tea (주요 조사료원과 쑥, 녹차의 반추위 건물 및 조단백질 소화율에 대한 분석)

  • Lee, Shin Ja;Lee, Su Kyoung;No, Jin Gu;Kim, Do Hyung;Lim, Jung Hwa;Moon, Yea Hwang;Lee, Sung Sill
    • Journal of agriculture & life science
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    • v.50 no.5
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    • pp.139-152
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    • 2016
  • The comparative in vitro and in situ analysis were conducted to evaluate the rumen degradability and physical structure of domestic roughage as rice straw, timothy, alfalfa, wormwood and green tea. The feedstuffs incubated with rumen fluid and was used to determine gas production, microbial growth rate and pH changes in an in vitro experiment. The gas production was increased during incubation times and was significantly(p<0.05) lower in green tea than other feedstuffs. The microbial growth rate in the feedstuffs was increased during incubation times. However, microbial growth rate was significantly(p<0.05) lower in wormwood and green tea than other feedstuffs. Ruminal pH was decreased during incubation times, and timothy was the lowest, and rice straw was the highest among feedstuffs. The disappearance rate of dry matter(DM) and crude protein(CP) in all feedstuffs were increased during incubation times and green tea was the highest(p<0.05) compared with other feedstuffs. In effective degradability, when rumen out-flow rate was assigned to 4%, wormwood showed the highest in DM, and alfalfa was the highest in CP. Whereas, green tea was the highest in both in situ DM and CP degradability. Many cilia on the surface and stoma of wormwood and stoma in green tea were observed by scanning electron microscopy. Microbes breaked down the cilia at the beginning and then degraded the surface in wormwood. In case of green tea, microbes attached to stoma. Therefore, wormwood and green tea have a potential value as ruminal feed stuffs.

Effects of Mixture of Different Maturity Groups on Dry Matter Yield and Nutritive Value of Oat and Rape in the Fall (가을철에 숙기가 다른 연맥과 유채 품종의 혼파가 사초의 건물수량 및 사료가치에 미치는 영향)

  • Lee, Sung Cheol
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.18 no.4
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    • pp.355-360
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    • 1998
  • This study was conducted to investigate the effects of mixture of different maturity groups on dry matter yield and nutritive value of oat(var. Foothill and Swan) and rape(var. Akela, Ramon and Ember) in the Fall at the Experimental field of Woosuk University, Wanju, in 1998. The results obtained are summarized as follows : 1. Plant height of Foothill was very slightly higher than that of Swan in oat, but there was no significant difference. Also, although there was no significant difference, there was high in plant height of Ember and Akela with Foothill, and Ramon with Swan. 2. Dry matter(DM) yield of Swan was very slightly higher than that of Foothill in oat, but there was no significant difference. Although there was no significant difference, dry matter yield of Akela was the highest in both oats. 3. Acid detergent fiber and neutral detergent fiber content of Swan were very slightly higher than that of Foothill in oat. And it was slightly higher in Akela or Ramon with Foothill, and it was greatly higher in Ember with Swan. 4. In vitro dry matter digestibility(IVDMD) content of Foothill was very slightly higher than that of Swan in oat. And it was slightly higher in Ember with Foothill, and it was slightly higher in Akela with Swan. Also, in vitro digestible dry matter(IVDDM) of Foothill was very slightly higher than that of Swan in oat. And it was slightly higher in Akela with Foothill and Swan. 5. Crude protein(CP) content and crude protein(CP) yield of Foothill were very slightly higher than that of Swan in oat. And it was slightly higher in Akela with Foothill and Swan. The results demonstrated that although there was not significant difference, the mixture of var, Foothill of oat and var, Akela of rape in the Fall was very good for nutritive value and DM yield.

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Seeding Rate and Planting Date Effects on Forage Performance and Quality of Spring Oats (연맥의 사초특성 및 품질에 미치는 파종량 및 파종기의 영향)

  • Kim, S.G.;Kim, J.D.;Park, H.S.;Kim, D.A.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.3
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    • pp.233-240
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    • 1999
  • Understanding of the relationships between seeding rate and planting date is important for production of spring oats during the fall season. Therefore, this experiment was conducted to investigate seeding rate and planting date effects on forage performance and quality of spring oats(Avena sativa L.) at Suweon in 1997. The experiment was arranged in a split plot design with three replications. The main plots consisted of different seeding rates such as 150, 200, and 250kg/ha. The subplots consisted of different planting dates of spring oats such as 15, 22, and 29 August and 5 September. Dry matter content of oats was significantly decreased(P<0.01) as the planting date was delayed except the planting date of 5 September(24.8%). Dry matter content of forage oats planted on 15, 22, and 29 August was 23.0, 21.5, and 20.5%, respectively. A seeding $rate\;{\times}\;planting$ date interaction for dry matter content was highly significant(P<0.01). Crude protein content of oats was significantly increased(P<0.01) from 15.6 to 27.9% as the planting was delayed, while a significant seeding $rate\;{\times}\;planting$ date interaction for crude protein content of oats was observed. Acid detergent fiber(ADF) and neutral detergent fiber(NDF) contents of oats increased with early planting(P<0.01), but there was no significant difference among the seeding rates. ln vitro dry matter digestibility(IVDMD) of oats was significantly increased(P<0.01) as the seeding rate was increased together with late planting. Average dry matter yield of oats decreased(P<0.01) from 2,647 to 615kg/ha as the planting was delayed, but there was no significant difference among the seeding rates. A seeding $rate\;{\times}\;planting$ date interaction was observed for average dry matter yield of oats that the seeding rate increase in this experiment showed better at early planting dates. Crude protein yield of oats increased from 171 to 410kg/ha as the planting was delayed, and a significant increases(P<0.01) for crude protein yield was found among the seeding rates. ln vitro digestible dry matter(IVDDM) yield of oats decreased(P<0.01) as the planting was delayed, but there was no significant difference among the seeding rates. A significant seeding $rate\;{\times}\;planting$ date interaction for lVDDM of oats was found. The results of this study indicate that both the seeding rate of 200kg/ha and early planting before 29 August would be recommendable for forage yield and quality of oats in Korea.

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Studies on Productivity and Nutrient Quality of Forage Rape (Brassica napus Subsp. oleifera) VII. Productivity of forage rape cv. Velox and its variation of nutrient quality during the growth period (사초용유채 ( Brassica napus Subsp. oleifera )의 생산성과 사료가치에 관한 연구 VII. 사초용 유채품종 Velox의 생산성 및 생육기간중의 영양가치의 변화)

  • ;Ichiro Goto
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.9 no.3
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    • pp.179-186
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    • 1989
  • According to the results from the experiments of selecting a suitable variety of forage rape and comparing productivity and nutrient quality between forage rape and oil seed rape, Velox appeared to be the most suitable variety in terms of productivity and nutrient quality at the southern area of Korea among the varieties used in the experiments. Consequently, Velox was grown under two different cultural methods, individual culture and population culture, and productivity and variation of nutrient quality during the growth period were observed and compared between the cultural methods. The results from the experiment are summarized as follows: 1. Although variations were great in plant fresh weight and plant dry matter weight among plants, individual culture showed significantly higher value in these characters at the 1 % level than population culture. Plant fresh weight and plant dry matter weight were largely dependent upon the amount of branches in case of individual culture. However, in population culture, they were equally dependent upon the amount of branches and main stem. There was no significant difference in dry matter percentage between cultural methods, and main stem showed highest dry matter percentage. 2. Content of crude protein was decreasing gradually as plants continued to grow. Individual culture showed higher content of crude protein than population culture from 90 to 120 days after sowing but vice versa from 180 days after sowing to flowering stage. Contents of fiber such as NDF, ADF, cellulose and lignin was low at the early stage of growth. It was increasing gradually as plants grew older and at the latter stage of growth plants under individual culture showed higher values in contents of fiber. 3. In vitro dry matter digestibility (IVDMD) of both stems and leaves was decreasing gradually as plants grew older. Plants under individual culture showed higher IVDMD of stems than plants under population culture, but no significant difference in IVDMD of leaves was observed between cultural methods.

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Evaluation of nutritive value of chestnut hull for ruminant animals using in vitro rumen fermentation (밤 가공 부산물의 반추가축용 사료 가치 평가: in vitro 반추위 배양)

  • Jeong, Sin-Yong;Jo, Hyeon-Seon;Park, Gi-Su;Kang, Gil-Nam;Jo, Nam-Chul;Seo, Seongwon
    • Korean Journal of Agricultural Science
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    • v.39 no.3
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    • pp.335-340
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    • 2012
  • During the manufacturing process of chestnut, 50% of biomass is produced as chestnut shell (CS) or chestnut hull (CH), a forestry by-product. Due to its high fiber content and economic benefit, there is a possibility of using chestnut hull as a supplement for a ruminant diet. Few studies, however, have been conducted on evaluating nutritive value of chestnut hull for ruminant animals. The objective of this study were thus to analyze chemical composition of CS, a by-product after the first processing of chestnut, and CH, a by-product after the second processing, and access in vitro rumen fermentation characteristics of them. For the in vitro fermentation using strained rumen fluid obtained from a fistulated Hanwoo steer, commercial total mixed ration (TMR) for dairy goat was used as a basal diet and was replaced with different proportions of chestnut shell and hull. A total number of 13 treatments were carried out in this study: 100% TMR, 100% CS, 100% CH, a mix with 50% CS and 50% of CH (MIX), TMR replaced with 5%, 10%, or 15% of CS, CH, or MIX, respectively. For each treatment, in vitro dry matter digestibility (IVDMD) and pH after 48 hours of rumen fermentation were measured. Gas production at 6, 12, 24, 48 hours of incubation was also analyzed. Compared to CH, CS contains higher level of fiber (NDF, ADF, lignin) and consequently has a lower amount of non-fiber carbohydrate, but no difference was observed in the other nutrients (i.e. crude protein, crude fat, and ash). IVDMD was significantly (p<0.05) the highest in 100% CH (71.97%) and the lowest in 100% CS (42.80%). Addition of CH by replacing TMR did not affect IVDMD, while an increase in the proportion of CS tended to decrease IVDMD. The total gas production after 48 hours of incubation and the rate of gas production were also the highest in 100% CH and the lowest in 100% CS (P<0.05). Likewise, the pH after 48 hours of fermentation was significantly (p<0.05) the lowest in 100% CH (6.33) and the highest in 100% CS (6.50), and no significant difference in gas production was observed when TMR was replaced with CS or CH up to 15% (P>0.05). In conclusion, CH may successfully be used for a supplement in a ruminant diet. The nutritive value of CS is relative low, but can replace, if not 100%, low quality forage. This study provides valuable information about the nutritive value of CS and CH. An in vivo trials, however, is needed for conclusively accessing the nutritive value of CS and CH.

Variety and phosphate fertilizer dose effect on nutrient composition, in vitro digestibility and feeding value of cowpea haulm

  • Ansah, Terry;Algma, Henry Ayindoh;Dei, Herbert Kwabla
    • Journal of Animal Science and Technology
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    • v.58 no.6
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    • pp.19.1-19.7
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    • 2016
  • Background: Cowpea (Vigna unguiculata [L.]) is a legume cultivated throughout most tropical countries and is valued as food and feed for human and livestock respectively. The search for an improved cowpea variety has been on-going with the aim of improving traits such as grain yield, drought and pest resistance. But no information exist on the feeding value of these improved varieties. Phosphate (P) fertilizer application is recommended to augment grain yield in grain legumes but data on the effect of P fertilizer on haulm quality is limited. Results: Two separate experiments were conducted to determine the effect of P fertilizer dose on the nutritive value of haulms from different cowpea varieties (V). In experiment 1, effect of three P doses (30, 60 and 90 kg $P_2O_5/ha$) on in vitro gas production (IVGP) characteristics, concentrations of digestible organic matter (DOM), crude protein (CP), acid detergent fiber (ADF) and neutral detergent fiber (NDF) of haulms from five cowpea varieties (Zaayura-SARC 4-75, Songotra-IT97K-499-35, Hewale-IT93K-192-4, IT99K 573-1-1 and Asomdwe-IT94K-410-2) were investigated using the $3(P){\times}5(V)$ factorial treatment arrangements in a completely randomized design. In experiment 2, the effects of two P doses (30 and 90 kg $P_2O_5/ha$) and two varieties (Zaayura-SARC 4-75 and Hewale-IT93K-192-4) on the voluntary feed intake, live weight, haematology and carcass characteristics of Djallonke lambs were also assessed using a $2(P){\times}2(V)$ factorial treatment arrangement. The $V{\times}P$ interaction significantly affected CP, NDF and ADF with CP concentration increasing with increase in P doses in Zaayura-SARC 4-75 and Asomdwe-IT94K-410-2. Whilst an increase (P < 0.05) in NDF was observed in Songotra-IT97K-499-35and Asomdwe-IT94K-410-2 as P doses increased, the other V only increased from P dose 30 to 60 kg/ha and declined at P dose 90 kg/ha. The ADF decreased (P < 0.05) with increase in P dose for all V with the exception of Songotra-IT97K-499-35. There was a significant V effect on DOM with the highest reported in Zaayura-SARC 4-75 (43.44 %). Daily DM intake, carcass length and blood urea nitrogen of the lambs were significantly affected by the V x P interaction. There was a significant V effect on globulin and P effect on live weight at slaughter, dressed weight, chuck, leg, loin, rib and flank and liver and lungs. Conclusion: It can be concluded that nutrient concentrations of cowpea haulms were positively influenced by different P dose and varieties with favorable effects on growth, haematology and carcass composition of lambs. Varieties Zaayura-SARC 4-75 and Hewale-IT93K-192-4 at P dose at 90 kg/ha are recommended to enhance growth performance and carcass yield of Djallonke lambs.

Nutritive Values and Quality of Sorghum-Sorghum Hybrid Bale and Trench Silage According to Those Parts (수수-수수 교잡종 원형곤포 및 트렌치 사일리지 부위별 사료가치 및 품질)

  • Choi, Ki-Choon;Ryu, Jai-Hyunk;Jung, Min-Woong;Park, Hyung-Su;Kim, Da-Hye;Kim, Cheon-Man;Kim, Mang-Jung;Kim, Jong-Geun;Kim, Won-Ho;Lim, Young-Chul;Choi, Gi-Jun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.3
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    • pp.221-228
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    • 2012
  • This study was performed to investigate the effects of various parts of sorghum-sorghum hybrid (SSH) silage on nutritive values and qualities of SSH round bale silage and trench silage. SSH "SS405" was harvested at heading stages and ensiled. Samples of SSH silage used in this study were collected in three different parts (outer, middle and inner). The contents of crude protein (CP) and neutral detergent fiber (NDF), and in vitro dry matter digestibility (IVDMD) in SSH round bale silage showed trends similar to SSH trench silage. However, the contents of acid detergent fiber (ADF) and total digestible nutrient (TDN) in SSH trench silage appeared slightly an increased trend, as compared to SSH round bale silage. The contents of CP in inner parts of both SSH round bale silage and trench silage were lower than those of Outer and middle parts (p<0.05). However, The contents of NDF, ADF and TDN, and IVDMD were not different between the parts of SSH silage. The content of lactic acid in SSH round bale silage was significantly decreased (p<0.05), as compared to SSH trench silage. The content of acetic acid appeared an decreased trends, but there is not significantly different between SSH round bale silage and trench silage. In addition, the contents of lactic acid, acetic acid and butyric acid were not different between the parts of SSH silage.

Determination of Nutritive Value of Wild Mustard, Sinapsis arvensis Harvested at Different Maturity Stages Using In situ and In vitro Measurements

  • Kamalak, Adem;Canbolat, Onder;Gurbuz, Yavuz;Ozkan, Cagri Ozgur;Kizilsimsek, Mustafa
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.9
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    • pp.1249-1254
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    • 2005
  • The aim of this study was to determine the effect of maturity stage on the nutritive value of wild mustard straw in terms of chemical composition, in situ, in vitro dry matter degradability and calculated ME. The nutritive values of wild mustard, Sinapsis arvensis hays harvested at three stages were evaluated by chemical composition, in vitro gas production and in situ dry matter degradation methods. Gas production or dry matter (DM) degradation were determined at 0, 3, 6, 12, 24, 48, 72 and 96 h and their kinetics were described using the equation p = a+b(1-e$^{-ct}$). Maturity had a significant effect on both the chemical composition and degradability of wild mustard. Neutral detergent fibre (NDF) and acid detergent fibre (ADF) (p<0.001) increased with increasing maturity whereas the crude protein (CP) (p<0.001) decreased. The gas produced after 96 h incubation ranged between 64.7 and 81.5 ml per 0.200 g of dry matter. The gas production (ml) at all incubation times and estimated parameters decreased with increasing maturity of wild mustard. The gas production at all incubation times and estimated parameters (a, b (a+b), metabolizable energy (ME) and organic matter digestibility (OMD)) were negatively correlated with NDF and ADF. The DM disappearance after 96 h incubation ranged between 50.8 and 76.1%. The in situ DM disappearance at all incubation times and estimated parameters decreased with increasing maturity of wild mustard. The in situ dry matter disappearance at all incubation times and some estimated parameters (c, a, b and effective dry matter degradability (EDMD)) were negatively correlated with NDF and ADF but positively correlated with CP. The nutritive value of wild mustard continually changed as it matured. Wild mustard, harvested at the proper stage of maturity offers considerable potential as a high quality forage for ruminants during the winter feeding period. The present study showed that if higher quality forage is an objective, wild mustard should be harvested at the early flowering stage.