• Title/Summary/Keyword: in vitro plantlet

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Improved Detection and Purification of Grapevine Leafroll-associated 3 Closterovirus Using Tissue Culture (포도 조직배양에 의한 Grapevine Leafroll-associated 3 Closterovirus의 증식과 검출효율 증대)

  • 김현란;정재동;정봉남;이봉춘;박진우;최용문
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.6
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    • pp.335-339
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    • 2001
  • Grapevine leafroll-associated 3 closterovirus (GLRaV-3) is phloem limited virus, and one of the most severe viral diseases found in Korea. However, nonhomogenous distribution and low concentration and seasonal variations of GLRaV-3 in grapevines remain as main problems which prevent the introduction and molecular biology or serology experiments. Virus-infected plantlet in vitro was obtained from node tissue cuttings, which was GLRaV-3 infected 'kyoho' vines. The amount of purified virus was highest in vitro plantlet. Moreover, viruses seem to be relatively homogeneously distributed in all organs including leaf, stem and callus derived from in vitro plantlets. RT-PCR detection using in vitro plantlet tissue as template was most effective. When comparing ELISA to RT-PCR, RT-PCR detection was 1,000 times as effective as ELISA. These results can be explained by improved quality such as tenderness or less tannins in plantlet in vitro. In conclusion, until infected herbaceous host will be available, tissue culture can be usefully adopted as a technique for a good source of GLRaV-3 closterovirus for further studies.

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The Effect of Pyroligneous Liquor and Coconut Water on Plantlet Multiplication and in Vitro Flowering of Dendrobium moniliforme (목초액 및 코코넛액이 석곡(Dendrobium moniliforme)의 유묘 증식과 기내 개화에 미치는 영향)

  • Jee, Sun-Ok;Cho, Dong-Hoon
    • Journal of Life Science
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    • v.15 no.5 s.72
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    • pp.739-742
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    • 2005
  • This experiment was carried out to clarify the effect of pyroligneous liquor and coconut water on plantlet multiplication and in vitro flowering of Dendrobium moniliforme. Plantlet growth and multiplication was good in 1.0 ml/L pyroligneous liquor treatment which was added to the basal media of 3 g/L hyponex and 4 g/L peptone ($H_{3} P_{4}$) containing 0.1 mg/L NAA and 1.0 mg/L kinetin. In the treatment of 30 ml/L coconut water showed good results on plantlet growth and multiplication. In vitro flowering showed highest rate in the treatment of 1.0 ml/L pyroligneous liquor and 30 ml/L coconut water which were added to the basal media of $H_{3} P_{4}$ containing 0.1 mg/L NAA and 1.0 mg/L kinetin.

Factors influencing somatic embryo maturation, high frequency germination and plantlet formation in Terminalia chebula Retz.

  • Anjaneyulu, C.;Giri, C.C.
    • Plant Biotechnology Reports
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    • v.2 no.2
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    • pp.153-161
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    • 2008
  • The factors influencing somatic embryo maturation, high frequency somatic embryo germination, and plantlet formation were studied in Terminalia chebula Retz. Maturation of somatic embryo were influenced by a number of factors such as in vitro culture passage, concentrations of sucrose, levels of abscisic acid (ABA), basal media and media additive combinations. Maximum frequency of somatic embryo maturation ($57.22{\pm}2.02$), was obtained on MS medium supplemented with 50 g/l sucrose. Different factors such as strengths of MS nutrients, plant growth regulators, media additives and their combinations controlling somatic embryo germination and plantlet formation were studied. High frequency of germination and plantlet formation ($58.80{\pm}1.47$) were achieved by subsequent subculture of mature somatic embryos on MS medium containing 30 g/l sucrose and 0.5 mg/l benzyl-adenine (BA). However, although duration of in vitro passage of the callus tissue was critical, contribution of the combinations of plant growth regulators and media additives showed nugatory effect on somatic embryo maturation and germination as evident from variable responses.

Study on the Clonal Multiplication of Zingiber mioga ROSC. through in vitro Culture of Shoot Apex. I. Effects of Basal Media and Growth Regulators on Plant Regeneration and Growth of Plantlet (양하(襄荷)의 경정배양(莖頂培養)에 관(關)한 연구(硏究) I. 기본부지(基本部地) 및 생장조절물질(生長調節物質)이 식물체(植物體) 재분화(再分化)와 유묘(幼苗)의 생장(生長)에 미치는 영향(影響))

  • Choi, Seong-Kyu;Seo, Young-Nam
    • Korean Journal of Medicinal Crop Science
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    • v.1 no.1
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    • pp.38-42
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    • 1993
  • The present study was carried out to assess the possibility of rapid multiplication of Zingiber mioga ROSC. through in vitro culture of shoot-apex. The factor investigated was effect of various growth regulators on shoot-apex culture. The shoot-apex cultured of M. S. (Murashige and Skoog) medium developed into plantlet in 12 Weeks. M. S. medium containing NAA at 05ppm and BA 5.0ppm was found to be optimal for growth of in vitro plantlet.

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In vitro Propagation using Shoot Tip Culture of Curcuma longa L. (울금의 경정배양에 의한 기내번식)

  • 최성규
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.6
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    • pp.438-441
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    • 2003
  • The present study was carried out to assess the possibility of rapid multiplication of Curcuma longa Linne through in vitro culture of shoot-apex. The factor investigated was effect of various growth regulators on shoot-apex culture. The shoot-apex cultured of MS(Murashige and Skoog) medium developed into plantlet in 16 Weeks. M.S. medium containing NAA at 0.5 ppm and BA 5.0 ppm was found to be optimal for growth of in vitro plantlet

Effect of biocide addition on plantlet growth and contamination occurrence during the in vitro culture of blueberry

  • Huh, Yoon Sun;Lee, Joung Kwan;Kim, Ik Jei;Kang, Bo Goo;Lee, Ki Yeol
    • Journal of Plant Biotechnology
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    • v.42 no.2
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    • pp.111-116
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    • 2015
  • Interest and great demand for blueberry (Vaccinium corymbosum) have increased, as V. corymbosum is now one of the most economically important crops in Korea. It is expected that blueberry production and the area planted for cultivation will increase consistently in the years ahead because of high profitability and the consumer's demand for healthy ingredients. Effective mass production of blueberry is urgently needed for commercial cultivation establishment, but a main limitation is lack of a propagation system that produces a disease-free plant material for commercial plantation. A large amount of research has focused entirely on developing tissue culture techniques for blueberry propagation. However, controlling fungal and bacterial contamination of woody plant material is extremely difficult. Our study was conducted to investigate the effect of biocide addition during the in vitro culture of blueberry on plantlet growth and contamination occurrence. Four biocides, including Plant Preservative Mixture ($PPM^{TM}$), vancomycin, nystatin and penicillin G, were used in varying concentrations during the in vitro propagation of blueberry. When nystatin was added into the medium at low concentrations, the overall growth of blueberry plantlets was retarded. Addition of vancomycin and penicillin G in high concentrations decreased contamination but induced plantlet mortality. On the other hand, when 1ml/L $PPM^{TM}$ was added, the growth characteristics of blueberry plantlets did not significantly differ from non-treatment (control), and the contamination occurrence rate was very low. From these results, we found that the addition of the appropriate biocide could provide an effective method to reduce contamination in the culture process, thereby raising in vitro production efficiency.

Variation of the Regenerated Plantlets from in Vitro Culture of Neoregeria carorinae 'Tricolor' and in Vivo Growth of Regenerated Plantlets (네오레게리아 기내배양시 변이발생과 기외 생육)

  • 정향영;한봉희;신학기;김의영
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.5
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    • pp.273-276
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    • 1995
  • In vitro propagation of Neoregeria carorinae 'Tricolor' was achieved by using immature flowers and lateral buds, and the plantlets from tissue culture were transplanted and cultivated in greenhouse. The picking times of explants to decrease disappearance of stripes, and in vivo the growth and flowering of regenerated plantlets as influenced by in vivo healed nun were investigated. The normal plantlet were obtained at a frequency of 67%, in the culture of immature flowers picked at 4 weeks after flower bud differentiation, while all leaf stripes disappeared in the culture of immature flowers picked 1 and 5 weeks after flower bud differentiation. In vivo growth of plantlet from immature flower buds was better than those from lateral buds, and the flowering of 27.8% showed in the greenhouse culture of plantlet from immature culture, but the plantlets from lateral buds did not flower at all. The plantlets rooted on the medium with 0.5 mg/L IBA were the most favorable in green house culture, and the kinds and concentrations of auxin in vitro did not have any influence on variation of plane cultured in greenhouse.

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The apical bud as a novel explant for high-frequency in vitro plantlet regeneration of Perilla frutescens L. Britton

  • Hossain, H.M.M. Tariq;Kim, Yong-Ho;Lee, Young-Sang
    • Plant Biotechnology Reports
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    • v.4 no.3
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    • pp.229-235
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    • 2010
  • In this study, we established an in vitro regeneration system to maximize the recovery of leafy perilla (Perilla frutescens L. Britton) plantlets as part of developing a molecular biotechnology-based metabolic engineering program for this crop plant. Hypocotyl segments including the apical buds were used as explants for the direct production of shoots without an interim callus phase. The number of shoots produced from the apical buds peaked within 3-4 weeks, and the shoots were subsequently cultured on Murashige and Skoog (MS) media supplemented with 2 mg $1^{-1}$ benzylaminopurine (BA). Spontaneous rhizogenesis was observed after 7-10 days of culture on MS media without hormonal additives. The rooted shoots developed into normal plants in soil after hardening on distilled water for 3-4 days. The average plantlet regeneration frequency was higher for the apical buds (64.33%) than for the top (15.66%), middle (4%), and basal (1.33%) segments of the hypocotyls. This regeneration system demonstrates a capacity for high-frequency plantlet recovery and thus should be considered for use in the genetic manipulation of leafy perilla.

Rapid Micropropagation of Hovenia dulcis Thunb. Through in vitro Stem Nodal Cultures

  • Park, Dong-Jin;Kang, Young-Min;Jung, Ha-Na;Min, Ji-Yun;Kim, Yong-Duck;Karigar, Chandrakant S.;Choi, Myung-Suk
    • Journal of Korean Society of Forest Science
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    • v.95 no.2
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    • pp.155-159
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    • 2006
  • An efficient method for in vitro propagation of the medicinal plant Hovenia duleis, was established. Plantlets for micropropagation of H. dulcis were obtained from in vitro germinated seeds. The effectiveness of various levels of cytokinins (BAP, Kinetin and TDZ) on multiple shoot formation from stem nodes was tested. BAP (1.0 mg/L) treatment induced highest number of multiple shoots. The growth pattern of plantlet on various culture media was undertaken. The shoot elongation was optimal on 2MS basal medium without growth regulators. The in vitro rooting ability of H. dulcis shoots was examined with two-auxins IAA and IBA. The IAA (1.0 mg/L) treatments induced earliest rooting with maximum number of roots and root growth. Rooted shoots were transferred directly to small pots with artificial soil and such established plant exhibited a normal growth pattern similar to wild plantlet.

Bioceramic Effects to Enhance Secondary Metabolites Production in Tissue Culture of Some Medicinal Plants

  • Kim, Yu-Jeong;Hwang, Baik;Ahn, Jun-Cheul
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.2
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    • pp.118-122
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    • 2004
  • We have investigated that a couple of soft ferrite ceramic powders having a spinal structure have shown the effect on growth and secondary metabolites production of some medicinal plants cultured in vitro. The addition of the ceramic powders as bare state to culture medium has stimulated the growth of Achyranthes japonica callus and plantlet, adventitious root of Hyoscyamus niger and Platycodon grandiflorum hairy root about 65, 75, 150 and 50%, respectively. Whereas Centella asiatica callus and plantlet, Scopolia parviflora hairy root, and Hyoscyamus albus adventitious root were not affected markedly. Moreover, the ceramic powder has enhanced the growth of H. niger adventitious roots even under conditions of irradiating alone without any direct contact between ceramic powder and media. Based on growth stimulation effect, the ceramic powders have enhanced the gross production of tropane alkaloid in H. niger adventitious root, and polyacetylene in P. grandiflorum hairy root about 35 and 30%, respectively.