• 제목/요약/키워드: in vitro germination

검색결과 274건 처리시간 0.028초

자당과 붕산이 구기자 화분 발아에 미치는 영향 (Effect of Sucrose and Boric Acid on In Vitro Pollen Germination of Boxthorn (Lycium chinense M.))

  • 김수동;민병훈;윤화모;이옥란
    • 한국약용작물학회지
    • /
    • 제21권5호
    • /
    • pp.348-352
    • /
    • 2013
  • This study was carried out to investigate the effect of sucrose, boric acid, and calcium nitrate in optimum pH and relative humidity on pollen germination and pollen tube growth of boxthorn (Lycium chinense M.). Medium containing 100mg/L of $H_3BO_3$ showed maximum pollen germination and pollen tube elongation, 64.8% and $920{\mu}m$. Additionally supplied calcium nitrate to the medium lowered pollen germination rate and pollen tube elongation. Here we report the optimum conditions for the pollen germination and pollen tube development was observed at 10% sucrose, 1.0% agar, pH 6.0, and 100% relative humidity.

In Vitro Inhibitory Activity of Cow Urine and Dung to Fusarium solani f. sp. cucurbitae

  • Basak, A.B.;Lee, Min-Woong;Lee, Tae-Soo
    • Mycobiology
    • /
    • 제30권1호
    • /
    • pp.51-54
    • /
    • 2002
  • This paper deals with the study on comparative efficacy and in vitro activity of cow urine and cow dung for controlling root rot disease of cucumber caused by Fusarium solani f. sp. cucurbitae Snyder & Hansen following slide germination and mycelial growth inhibition tests. Results showed that both germination of conidia and the percentage inhibition of mycelial growth decreased or suppressed and varied greatly with respect to different hour and days of incubation and kind of bio-matters. In between two bio-matters cow urine was found more effective than that of cow dung in conidial germination. No germination of conidia was recorded after one hour of incubation in any medium whereas in cow urine germination of conidia was not also observed even after 2 hours of incubation. After 7 hours of incubation out of 200 conidia of F. solani f. sp. cucurbitae, 28 in cow urine and 64 in cow dung were germinated while in control a total germinated conidia was 185. In case of percentage inhibition of conidial germination the highest percentage(100%) was recorded in cow urine after 2 hours of incubation followed by 3 hours(96.0%), 4 hours(91.0%) and 6 hours(89.4%). During the test on inhibition of mycelial growth, the highest percentage(62.8%) was recorded in cow urine potato dextrose agar(CUPDA) medium tested after 4 days of incubation, followed by 3 days(60.5%), 5 days(56.5%) and 2 days(55.0%). In this test cow dung potato dextrose agar(CDPDA) had less efficacy in suppression of the percentage inhibition of mycelial growth.

Effect of Gibberellin and Light on Germination of Seeds in Codonopsis lanceolata Benth

  • Ghimire, Bimal Kumar;Shin, Chul-Min;Li, Cheng-Hao;Chung, Ill-Min;Lee, Dong-Wook;Kim, Hee-Young;Kim, Na-Young;Lim, Jung-Dae;Kim, Jae-Kwang;Kim, Myong-Jo;Cho, Dong-Ha;Lee, Sun-Joo;Yu, Chang-Yeon
    • 한국약용작물학회지
    • /
    • 제14권5호
    • /
    • pp.303-306
    • /
    • 2006
  • Seed of Codonopsis lanceolata exhibits low germination due to impermeable seed coat. Prolonged seed dormancy can be overcome by the application of gibberellins, as it promotes growth of the embryo and weakens the structures surrounding of embryo. The effects of photoperiod, sugar and gibberellin concentration were investigated at constant temperature for 12 days and 22 days in vitro and invivo conditions respectively. The rate of germination of seeds of Codonopsis lanceolata in wet filter paper in both complete dark and light treatments was significantly lower than that of seed treated with $GA_3$. It clearly indicates that there is significant coat imposed dormancy in the seed of Codonopsis lanceolata. The rate of germination in vivo condition was lower than that of the in vitro condition supplemented with $GA_3$ Thus, the physical dormancy due to impermeable seed coat and low level of endogenous gibberellins in the seed was the cause of poor germination rate in Codonopsis lanceolata.

Enhancement of seed germination and microbial disinfection on ginseng by cold plasma treatment

  • Lee, Younmi;Lee, Young Yoon;Kim, Young Soo;Balaraju, Kotnala;Mok, Young Sun;Yoo, Suk Jae;Jeon, Yongho
    • Journal of Ginseng Research
    • /
    • 제45권4호
    • /
    • pp.519-526
    • /
    • 2021
  • Background: This study aimed to investigate the effect of cold plasma treatment on the improvement of seed germination and surface sterilization of ginseng seeds. Methods: Dehisced ginseng (Panax ginseng) seeds were exposed to dielectric barrier discharge (DBD) plasma operated in argon (Ar) or an argon/oxygen mixture (Ar/O2), and the resulting germination and surface sterilization were compared with those of an untreated control group. Bacterial and fungal detection assays were performed for plasma-treated ginseng seeds after serial dilution of surface-washed suspensions. The microbial colonies (fungi and bacteria) were classified according to their phenotypical morphologies and identified by molecular analysis. Furthermore, the effect of cold plasma treatment on the in vitro antifungal activity and suppression of Cylindrocarpon destructans in 4-year-old ginseng root discs was investigated. Results: Seeds treated with plasma in Ar or Ar/O2 exhibited a higher germination rate (%) compared with the untreated controls. Furthermore, the plasma treatment exhibited bactericidal and fungicidal effects on the seed surface, and the latter effect was stronger than the former. In addition, plasma treatment exhibited in vitro antifungal activity against C. destructans and reduced the disease severity (%) of root rot in 4-year-old ginseng root discs. The results demonstrate the stimulatory effect of plasma treatment on seed germination, surface sterilization, and root rot disease suppression in ginseng. Conclusion: The results of this study indicate that the cold plasma treatment can suppress the microbial community on the seed surface root rot in ginseng.

Interspecific Hybridization between Populus caspica L. × P. deltoids L 62/154 Using in vitro Embryo Development and Germination

  • Ali, Jafari mofidabadi;Mansooreh, Kamandloo;Hamid, Selamti
    • Journal of Forest and Environmental Science
    • /
    • 제33권3호
    • /
    • pp.197-201
    • /
    • 2017
  • Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at ${\alpha}=0.01%$. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at ${\alpha}=0.05%$. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.

이탈리안 라이그라스 품종별 NaCl 처리가 발아 및 초기생육에 미치는 영향 (Effects of Sodium Chloride Treatment on Seed Germination and Seedling Growth of Italian Ryegrass Cultivars)

  • 이상훈;최기준;이동기;문진용;김기용;지희정;박형수;이기원
    • 한국초지조사료학회지
    • /
    • 제34권2호
    • /
    • pp.108-113
    • /
    • 2014
  • 이탈리안 라이그라스의 발아 및 초기 생육단계에서 내염성 수준 정도를 조사하기 위하여 in vitro 조건에서 다양한 염농도 조건에서 그린팜, 플로리다80, 코원어리 및 화산101호 품종을 이용하여 염해에 대한 반응을 발아율과 산화 스트레스 관련 효소들의 활성을 통해 조사하였다. 저농도의 NaCl 처리구에서는 모든 품종에서 발아율의 차이가 1~3% 정도 감소하는 경향을 보였으며 250 mM 이상의 NaCl 처리구에는 화산101호 품종을 제외한 모든 품종에서 50% 이상의 발아율 감소를 보였다. 또한, NaCl 농도가 높아질수록 발아에 걸리는 시간이 지연되었으며, 350 mM NaCl이 첨가된 처리구에는 화산101호 품종만이 치상 10일후에 발아가 시작하여 12% 정도 발아하였다. NaCl 농도에 따른 shoot과 root의 길이와 생초와 건조 후 무게의 변화도 만생종으로 갈수록 더 생육이 우수한 것으로 타나났다. 품종별간의 잎 조직에서 항산화효소 활성을 분석 결과 고염 조건 (150 mM NaCl)에서 POD 활성은 대조군과 유사한 수준으로 확인 되었다. 반면 APX 활성은 플로리다80과 코윈어리 품종에서는 증가하는 경향을, 화산101 품종에서는 감소하는 경향이 나타났으나 유의적인 수준에서 차이는 확인되지 않았다. 반면 그린팜 품종에서는 유의적인 수준에서 고염 처리에 의한 APX 활성이 감소되는 경향이 나타났다. 지속적으로 in vitro screening을 통해 선발된 계통들은 향후 특성평가와 인공교배를 통해 내염성 이탈리안 라이그라스 개발에 필요한 유용한 육종모재로 활용될 수 있을 것으로 판단된다.

Rapid in vitro Germination of Zygotic Embryos via Endosperm Removal in Eleutherococcus senticosus

  • You Xiang-Ling;Choi Yong-Eui;Yi Jae-Seon
    • Journal of Plant Biotechnology
    • /
    • 제7권1호
    • /
    • pp.75-80
    • /
    • 2005
  • Eleutherococcus senticosus (also called Acanthopanax senticosus), belonging to Araliaceae family, has been used as an important medicinal woody plant. Mature seeds of Eleutherococcus senticosus have rudimentary (extremely immature) zygotic embryos and require a long-term stratification for about 18 months to induce germination. Here, through the methods of endosperm removal and other exogenous treatments, we investigated the factors for inducing rudimentary embryos by in vitro culture, Rudimentary zygotic embryos in seeds were at globular to heart-shaped stage at about $250{\mu}m$ in length just after harvest of fruits. When the seeds without testa were cultured on 1/2 MS (Murashige and Skoog 1962) medium, they did not germinate regardless of medium and sucrose concentrations but the removal of endosperm tissue markedly stimulated the growth of rudimentary zygotic embryos. The embryo reached ear-lier maturation, once when the endosperm surrounding the rudimentary embryos was removed. Rudimentary zygotic embryos developed cotyledons within 3 weeks of culture after endosperm emoval. However, post-mature zygotic embryos failed to germinate though they were morphologically normal, indicating another dormancy of embryos. $GA_3\;(2.0\;\cal{mg/L})$ and/or charcoal ($0.2\%$) treatment rapidly enhanced the germination of zygotic embryos. These results suggest that E. senticosus seeds have double dormancy; i. e. morphological rudimentary dormancy influenced by surrounding endosperm and physiological dormancy after post-maturation of zygotic embryos. Based on the above findings, we established the rapid germination of rudimentary zygotic embryos by in vitro culture of excised seeds with endosperm removal and $GA_3$ treatment.

In vitro Pollen Performance of Pinus densiflora and P. rigida: Temperature and Medium Nutrient Effects

  • Lee, Young-Keun;Kim, Yong-Ok;Booth, Thomas;Lee, Eun Ju
    • The Korean Journal of Ecology
    • /
    • 제25권6호
    • /
    • pp.389-393
    • /
    • 2002
  • Effects of environmental factors on in vitro pine pollen performance were investigated. Pinus densiflora and P. rigida pollen grains collected at Mt. Kwanak, Korea were used. Three environmental factors, such as pollen storage temperature, pollen culture temperature and nutrient condition in medium, were tested. To determine the storage temperature effects on pollen viability, pine pollen was stored at $-70^{\circ}C$, $-12^{\circ}C$, $4^{\circ}C$ and $22^{\circ}C$. Pollen viability was substantially extended at the storage temperatures of $-12^{\circ}C$ and $4^{\circ}C$ for more than 300 days. To elucidate the culture temperature effects on pine pollen germination and tube growth, pollen grains were cultured at the temperatures from $5^{\circ}C$ to $40^{\circ}C$ at $5^{\circ}C$intervals. The germination rate and tube growth were highest at $25^{\circ}C$ and decreased above $30^{\circ}C$. To investigate boron and sucrose effects on pollen tube growth, the pollen was cultured at different sucrose and boric acid concentrations. Germination rate was optimal in germination medium containing 3 or 5$\%$ sucrose with 0.01 $\%$ boric acid. These results indicate that the pine pollen can be stored for considerable length of time without noticeable loss of viability at storage temperature below or near $0^{\circ}C$. Optimal germination medium conditions were established for pine pollen. Therefore, pine pollen can be used for many biological and environmental monitoring researches.

Bacillus stearothermophilus YC4194에 의한 Pythium 모잘록병의 생물학적 방제 (Biological control of Pythium damping-off of cucumber by Bacillus stearothermophilus YC4194)

  • 양현숙;손황배;정영륜
    • 식물병연구
    • /
    • 제8권4호
    • /
    • pp.234-238
    • /
    • 2002
  • Bacillus stearothermophilus YC4l94는 in vitro 와 in vivo 에서 병원균 Pythium aphanidermatum에 대한 억제 효과를 보였다 in vitro 실험에서 P. aphanidermatum의 유주포자낭 형성과 피낭포자 발아를 억제하였을 뿐 아니라 식물체에 적접 길항균 제제 처리 시 50% 이상의 방제가를 보였다. 그리고 다른 화학 살균제와 비슷한 방제효과를 보여 미생물 제제로서의 개발 가능성을 보여주었다.

광, 온도 및 GA 침지처리가 한지형 잔디의 종자발아에 미치는 영향 (The Effect of Light, Temperature and GA Soaking Treatment on Seed Germination of Cool Season Turfgrasses)

  • 현상민;강훈;소인섭;김동일
    • 아시안잔디학회지
    • /
    • 제9권3호
    • /
    • pp.253-260
    • /
    • 1995
  • This study is aimed to understand the effects of temperature, light and GA soaking treatment on the seed germination of perennial ryegrass 'Dandy', creeping hentgrass 'Penlinks', tall fescue 'Arid' and Kentucky hluegrass 'Nasaw'. These experiments are carried in vitro, and their results obtained are summarized as the followings ; The optimum temperature for seed germination of perennial ryegrass 'Dandy' was found to he ranged at 20~30$^{\circ}C$, and the optimum temperature for germination of creeping bentgrass 'Penlinks', tall fescue 'Arid' and kentucky bluegrass 'Nasaw' seeds was found to be ranged at 20~25$^{\circ}C$, whereas the germination percentage under the below or above temperature of this range decreased. The germination of perennial ryegrass 'Dandy' and tall fescue 'Arid' seeds showed no effect on light, and while the germination percentage of creeping hentgrass 'Penlinks' increased more highly under the dark condition than under the light condition. The germination percentage decreased according to the higher temperature. The germination of Kentucky bluegrass 'Nasaw' seeds increased more highly under the dark condition than under the light condition at 20~25$^{\circ}C$, whereas the germination percentage under the dark condition at 15$^{\circ}C$ and 30$^{\circ}C$ decreased. In case of GA soaking treatment of seeds, all the cool season turfgrasses tested showed their own promoting effects on seed germination. The early germination of tall fescue 'Arid' was good without the soaking times. Creeping bentgrass 'Penlinks' and perennial ryegrass 'Dandy' had re-spectively the highest germination percentage at 10min and 1hr soaking treatment, and the longer the soaking time, the lesser the germination percentage. Kentucky bluegrass 'Nasaw' obtained the best results at 24has soaking treatment, and the longer the soaking time, the better the ger-mination percentage.

  • PDF