Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Enhancement of seed germination and microbial disinfection on ginseng by cold plasma treatment

  • Lee, Younmi (Department of Plant Medicals, Andong National University) ;
  • Lee, Young Yoon (Department of Plant Medicals, Andong National University) ;
  • Kim, Young Soo (Department of Plant Medicals, Andong National University) ;
  • Balaraju, Kotnala (Agricultural Science & Technology Research Institute, Andong National University) ;
  • Mok, Young Sun (Department of Chemical and Biological Engineering, Jeju National University) ;
  • Yoo, Suk Jae (Plasma Technology Research Center, National Fusion Research Institute) ;
  • Jeon, Yongho (Department of Plant Medicals, Andong National University)
  • Received : 2020.08.03
  • Accepted : 2020.12.07
  • Published : 2021.07.01
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Abstract

Background: This study aimed to investigate the effect of cold plasma treatment on the improvement of seed germination and surface sterilization of ginseng seeds. Methods: Dehisced ginseng (Panax ginseng) seeds were exposed to dielectric barrier discharge (DBD) plasma operated in argon (Ar) or an argon/oxygen mixture (Ar/O2), and the resulting germination and surface sterilization were compared with those of an untreated control group. Bacterial and fungal detection assays were performed for plasma-treated ginseng seeds after serial dilution of surface-washed suspensions. The microbial colonies (fungi and bacteria) were classified according to their phenotypical morphologies and identified by molecular analysis. Furthermore, the effect of cold plasma treatment on the in vitro antifungal activity and suppression of Cylindrocarpon destructans in 4-year-old ginseng root discs was investigated. Results: Seeds treated with plasma in Ar or Ar/O2 exhibited a higher germination rate (%) compared with the untreated controls. Furthermore, the plasma treatment exhibited bactericidal and fungicidal effects on the seed surface, and the latter effect was stronger than the former. In addition, plasma treatment exhibited in vitro antifungal activity against C. destructans and reduced the disease severity (%) of root rot in 4-year-old ginseng root discs. The results demonstrate the stimulatory effect of plasma treatment on seed germination, surface sterilization, and root rot disease suppression in ginseng. Conclusion: The results of this study indicate that the cold plasma treatment can suppress the microbial community on the seed surface root rot in ginseng.

Keywords

Acknowledgement

This work was supported by the Basic Science Research Program through the National Research Foundation funded by the Ministry of Science, ICT and Future Planning, Korea (Grant No. 2016R1A2A2A05920703).

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