• Title/Summary/Keyword: in situ strength

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Ex-situ conservation and cytotoxic activity assessment of native medicinal orchid: Coelogyne stricta

  • Thapa, Bir Bahadur;Thakuri, Laxmi Sen;Joshi, Pusp Raj;Chand, Krishna;Rajbahak, Sabari;Sah, Anil Kumar;Shrestha, Resha;Paudel, Mukti Ram;Park, So Young;Pant, Bijaya
    • Journal of Plant Biotechnology
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    • v.47 no.4
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    • pp.330-336
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    • 2020
  • Ex-situ conservation of the ornamental and medicinal orchid, Coelogyne stricta, was performed by mass propagation using seed culture. Propagation stages were optimized using full- and half-strength solidified MS medium with different phytohormones. Maximum seed germination (88 ± 0.5% over 6 weeks of culture) was achieved on half-strength MS medium supplemented with 15% coconut water. Maximum shoot numbers were found on full-strength MS medium supplemented with 1 mg/L BAP, 2 mg/L Kinetin, and 10% coconut water, while the longest root was developed on full-strength MS medium with 1.5 mg/L IBA. A 2:1:1 combination of coco-peat, pine bark, and sphagnum moss was found to be a suitable potting mixture resulting in 80% seedling survivability. The cytotoxic activity of extracts of both wild plants and in vitro-developed protocorms was determined using an MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay on a cervical cancer cell line. The wild plant extract inhibited the growth of 41.99% of cells, showing that this extract has moderate cytotoxic activity toward cervical cancer cells.

Estimation of Undrained Shear Strength of Very Soft Clay with the Slump Test (슬럼프 실험에 의한 초연약점토의 비배수전단강도 산정)

  • Noh, Tae-Kil;Lee, Song
    • Journal of the Korean Geotechnical Society
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    • v.25 no.2
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    • pp.17-24
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    • 2009
  • Undrained shear strength is estimated from laboratory tests generally, but the very soft or fluid material is generally incompatible with the test setup. In-situ methods require test to be accomplished at discrete time intervals, which does not provide a method to predict strength increment as a function of time for an ongoing project. Therefore, correlation between slump test value and undrained shear strength was derived through the regression analysis of slump test and laboratory vane shear test results. For the reliability of derived correlation equation statistical analysis using the t-distribution was performed and the comparison between the results of in-situ test and laboratory experiments demonstrated the applicability of the derived correlation.

Development of Triaxial Cells Operable with In Situ X-ray CT for Hydro-Mechanical Laboratory Testing of Rocks (원위치 X-ray CT 촬영이 가능한 암석의 수리-역학 실험용 삼축셀 개발)

  • Zhuang, Li;Yeom, Sun;Shin, Hyu-Soung
    • Journal of the Korean Geotechnical Society
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    • v.36 no.9
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    • pp.45-55
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    • 2020
  • X-ray computed tomography (CT) is very useful for the quantitative evaluation of internal structures, particularly defects in rock samples, such as pores and fractures. In situ CT allows 3D imaging of a sample subjected to various external treatments such as loading and therefore enables observation of changes that occur during the loading process. We reviewed state-of-the-art of in situ CT applications for geomaterials. Two triaxial cells made using relatively low density but high strength materials were developed aimed at in situ CT scanning during hydro-mechanical laboratory testing of rocks. Preliminary results for in situ CT imaging of granite and sandstone samples with diameters ranging from 25 mm to 50 mm show a resolution range of 34~105 ㎛ per pixel pitch, indicating the feasibility of in situ CT observations for internal structural changes in rocks at the micrometer scale. Potassium iodide solution was found to improve the image contrast, and can be used as an injection fluid for hydro-mechanical testing combined with in situ CT scanning.

Synthesis and Properties of Nylon 6/PEG Random Block Copolymer/Clay Nanocomposite via in situ Polymerization (in situ중합을 통한 나일론 6-PEG 랜덤공중합체/점토 나노복합체의 합성 및 물성)

  • Angelica S. Lopez;Pio Sifuentes;Kim, Kap-Jin
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.04a
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    • pp.72-74
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    • 2003
  • There has been extensive interest in the development of new nanocomposites. One kind of these systems is the hybrid based on organic polymers and inorganic minerals consisting of layered silicates. Some properties like stiffness, strength, barrier properties, thermal, and oxidative stability can be improved by the presence of the filler in the polymeric matrix[1]. It is reported that, in the nylon 6/clay nanocomposites, the modulus is increased, but impact strength and elongation at break are drastically decreased. (omitted)

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Side resistance of rock socketed drilled shafts considering in situ rock mass condition (현장조건을 고려한 현장타설말뚝의 단위주면마찰력)

  • Sagong, Myung;Paik, Kyo-Ho
    • Proceedings of the KSR Conference
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    • 2004.06a
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    • pp.967-973
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    • 2004
  • Rock socketed drilled shafts transfer significant portion of structural loads at the socketed part. Therefore, a proper design of side and base resistances of a shaft at the socket is a major concern for the geotechnical engineers. In this study, we modified the Hoek-Brown criterion to estimate side resistance of rock socketed drilled shafts. Earlier method to compute side resistance of a shaft is linear or power functions of intact rock masses. However, side resistance is mobilized like shearing which influenced by the mechanical properties of concrete and rock masses, adhesion of rock/concrete interface, roughness of rock socket. Therefore, a single coefficient or power of uniaxial compressive strength of intact rock cannot provide accurate values of side resistance in a wide range of the uniaxial compressive strength. A new approach proposed in this study can consider in situ rock mass condition (frequency or discontinuities, weathering condition), and rock types thus, it has a wider applicability than the earlier models.

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Uncertainy Analysis of Shear Strength Characteristics of Marine Soils (해성점토의 강도특성에 대한 불확실성 분석)

  • 이강운;채영수;윤길림;백세환
    • Proceedings of the Korean Geotechical Society Conference
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    • 2001.03a
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    • pp.215-222
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    • 2001
  • Uncertainty study of shear strength characteristics of the marine clays was carried out based ell In-situ tests and laboratory tests on tile south-east coastal region of the Korean peninsula. Theoretical analyses were studied using both tile spherical cavity expansion theory in finite soil mass and the strain path method to determine tile cone factor using the undrained shear strengths obtained by in-situ tests, and the empirical methods in accordance with the ultimate resistance theory were also discussed. Analysis show that the empirical methods suggest more reasonable value than that of theoretical methods in terms of comparing the cone factor estimated using linear regression and frequency distribution analyses. The cone factors obtained by the empirical methods are 18, 15, and 6 respectively, from the results of total cone resistance, effective cone resistance, and excess porewater cone resistance method, and the estimated were similar to those of previous researcher's.

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Development of Geotube for Practical Use (지오튜브공법의 실용화방안에 관한 연구)

  • 신은철;오영인;이희재
    • Proceedings of the Korean Geotechical Society Conference
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    • 2000.03b
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    • pp.504-511
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    • 2000
  • Geotube is a tube made of permeable but soil-tight geotextile, hydraulically filled with soil include dredged sand and mud, which has been successfully applied in hydraulic and coastal engineering projects. This method is getting popular and used a lot in the advanced countries of the world because of economical, useful, and enable to store and isolate contaminated materials as obtained by harbor dredging. Laboratory and pilot scale in-situ tests were performed to determine the design methodology and construction procedures. From the results of laboratory and in-situ model tests, the retention ratio of solid particle is minimum 86% and minimum permeability and tensile strength of geotextile is ${\alpha}$${\times}$10$\^$-2/ and 20 t/m, respectively. Also, based on the environmental model test results, it can be concluded that this method does meet the Korean EPA standards.

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Development of High Performance Shotcrete for Permanent Shotcrete Tunnel Linings II(II: Application of high-early strength cement in sump water condition) (Permanent Shotcrete Tunnel Linings 구축을 위한 고성능 숏크리트 개발 II (II: 용수부에서의 조강시멘트 적용))

  • 박해균;이명섭;김재권;안병제
    • Proceedings of the Korea Concrete Institute Conference
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    • 2002.10a
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    • pp.695-702
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    • 2002
  • Shotcrete (or Sprayed concrete) has been used as an important support material in New Austrian Tunnelling Method (NATM). Since the mid of 1990, permanent shotcrete tunnel linings such as Single-shell, NMT (Norwegian Method of Tunnelling) has been constructed in many countries for reducing the construction time and lowing construction costs instead of conventional in-situ concrete linings. Among essential technologies for successful application of permanent shotcrcte linings, high performance shotcrete providing high strength, high durability, better pumpability has to be developed in advance as an integral component. This paper presents the Ideas and first experimental attempts to increase early strength and bond strength of wet-mixed Steel Fiber Reinforced Shotcrete(SFRS) in sump water condition. In order to increase early strength, a new approach using high-early strength cement with liquid alkali-free accelerator has been investigated From the results, wet-mix SFRS with high-early strength cement and alkali-free accelerator exhibited excellent early strength improvement compared to the ordinary portland content and good bond strength even under sump water condition.

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Effect of Curing Temperature and Aging on the Mechanical Properties of Concrete (II) -Evaluation of Prediction Models- (콘크리트의 재료역학적 성질에 대한 양생온도와 재령의 효과(II) -예측 모델식을 중심으로-)

  • 한상훈;김진근;양은익
    • Journal of the Korea Concrete Institute
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    • v.12 no.6
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    • pp.35-42
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    • 2000
  • In paper I, the relationships between compressive strength and splitting tensile strength or modulus of elasticity were proposed. In this paper, new prediction model is investigated from estimating splitting tensile strength and modulus of elasticity with curing temperature and aging without compressive strength. New prediction model is based on the model which was proposed to predict compressive strength, and splitting tensile strength and modulus of elasticity calculated by this model are compared with experimental values of paper I. To evaluate in-situ applicability of the model, strength and modulus of elasticity tested with variable temperatures are estimated by the prediction model. The prediction model reasonably estimates the strength and the modulus of elasticity of type I and V cement concretes tested in paper I and experimental results with variable temperature tested in this paper.

Evaluation of ground characteristics near underground rainfall storage facilities using shear wave velocity (전단파 속도를 이용한 지하 저류조 주변 지반특성 평가)

  • Jo, Seon-Ah;Oh, Tae-Min;Cho, Gye-Chun
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.16 no.2
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    • pp.225-236
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    • 2014
  • Shear wave velocity was used to estimate the geotechnical characteristics (void ratio and shear strength) of ground near an underground rainfall storage facility. An oedometer cell was utilized to measure the shear wave velocity and the displacement of specimens. Shear strengths were obtained by direct shear tests. The relationships along the shear wave velocity, void ratio, and shear strength were verified and used to infer the shear strength profile with the depth. In addition, changes in shear strength due to the construction of the underground rainfall storage system were estimated using the suggested method. The results show that the in-situ shear strength deduced from the shear wave velocity-void ratio-shear strength relationship is in good agreement with that obtained from an in-situ investigation (SPT).