• Title/Summary/Keyword: in situ process

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Quality and Physiological Characteristics of Pinus densiflora Seeds Under Different Storage Periods and Temperatures (장기 저장과 저장 온도에 따른 소나무 종자 품질과 생리적 특성)

  • Gu, Da-Eun;Ku, Ja-Jung;Han, Sim-Hee
    • Journal of Korean Society of Forest Science
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    • v.111 no.3
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    • pp.418-427
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    • 2022
  • Our aim was to evaluate the quality of pine seeds after long-term storage and to analyze the correlation between germination and physiological characteristics. Therefore, we investigated the germination and physiological characteristics of seeds stored for different periods and storage temperatures. Pine seeds lost little viability and vigor after 12 years of storage at 4℃; it is even possible to prolong the storage period at -18℃. The deterioration process operating through long-term storage was characterized by a loss of seed vigor before a loss in seed viability. Electrical conductivity of the seed leachate was significantly higher in seeds that completely lost their viability, and was also found to be significantly correlated with the germination percentage, T50, mean germination time, and germination speed. Among the minerals leaked to the leachate, K had the highest concentration, followed by Na, Ca, Cu, Mg, and Fe, while Mn and Zn were not detected. Concentrations of K, Ca, Cu, Mg, and Fe in the leachate differed significantly by treatment, and in the cases of K, Ca, Cu, Na, and Mg, we observed large differences in the leachate between the seeds that were collected in 2003 and those collected in other years.. Germination percentage was significantly negatively correlated to the concentrations of Ca, Cu, K, Mg, and Na. The coefficient of uniformity of germination was significantly negatively correlated to Cu concentration. The other germination characteristics were not significantly correlated with the mineral concentration. Therefore, the mineral concentrations of pine seed leachate were not reliable indicators of seed deterioration when the differences in quality between seed lots were small. However, electrical conductivity could be an indicator of seed viability and vigor of pine seeds under long-term storage.

CHARACTERISTICS OF HETEROEPITAXIALLY GROWN $Y_2$O$_3$ FILMS BY r-ICB FOR VLSI

  • Choi, S.C.;Cho, M.H.;Whangbo, S.W.;Kim, M.S.;Whang, C.N.;Kang, S.B.;Lee, S.I.;Lee, M.Y.
    • Journal of the Korean institute of surface engineering
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    • v.29 no.6
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    • pp.809-815
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    • 1996
  • $Y_2O_3$-based metal-insulator-semiconductor (MIS) structure on p-Si(100) has been studied. Films were prepared by UHV reactive ionized cluster beam deposition (r-ICBD) system. The base pressure of the system was about $1 \times 10^{-9}$ -9/ Torr and the process pressure $2 \times 10^{-5}$ Torr in oxygen ambience. Glancing X-ray diffraction(GXRD) and in-situ reflection high energy electron diffracton(RHEED) analyses were performed to investigate the crystallinity of the films. The results show phase change from amorphous state to crystalline one with increasingqr acceleration voltage and substrate temperature. It is also found that the phase transformation from $Y_2O_3$(111)//Si(100) to $Y_2O_3$(110)//Si(100) in growing directions takes place between $500^{\circ}C$ and $700^{\circ}C$. Especially as acceleration voltage is increased, preferentially oriented crystallinity was increased. Finally under the condition of above substrate temperature $700^{\circ}C$ and acceleration voltage 5kV, the $Y_2O_3$films are found to be grown epitaxially in direction of $Y_2O_3$(1l0)//Si(100) by observation of transmission electron microscope(TEM). Capacitance-voltage and current-voltage measurements were conducted to characterize Al/$Y_2O_3$/Si MIS structure with varying acceleration voltage and substrate temperature. Deposited $Y_2O_3$ films of thickness of nearly 300$\AA$ show that the breakdown field increases to 7~8MV /cm at the same conditon of epitaxial growing. These results also coincide with XPS spectra which indicate better stoichiometric characteristic in the condition of better crystalline one. After oxidation the breakdown field increases to 13MV /cm because the MIS structure contains interface silicon oxide of about 30$\AA$. In this case the dielectric constant of only $Y_2O_3$ layer is found to be $\in$15.6. These results have demonstrated the potential of using yttrium oxide for future VLSI/ULSI gate insulator applications.

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Applications of Focused Ion Beam for Biomedical Research (의생물 연구 분야에서 집속이온빔장치의 응용)

  • Kim, Ki-Woo;Baek, Saeng-Geul;Park, Byung-Joon;Kim, Hyun-Wook;Rhyu, Im-Joo
    • Applied Microscopy
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    • v.40 no.4
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    • pp.177-183
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    • 2010
  • A focused ion beam (FIB) system produces a beam of positive ions (usually gallium) which are heavier than electrons and can be focused by electrostatic lenses into a spot on the specimen. With its ability milling of the specimen material by 10 to 100 nm with each pass of the beam, FIB is widely adopted in materials science, semiconductor industry, and ceramics research. Recently, FIB has been increasingly employed in the field of biomedical sciences. Here we provide a brief introduction to FIB and its applications for a wide variety of biomedical research. The surface of specimen can be in situ processed and quasi-real time visualized by two beam combination of FIB and field emission scanning electron microscope (FESEM). Due to its milling process, internal structures can be exposed and analyzed: yeast cells, fungus-inoculated wheat leaf, mannitol particles in inhalation aerosols, and oyster shell. Serial blockface tomography with the system kindles 3-dimensional reconstruction researches in the realm of nervous system and life sciences. Two-beam system of FIB/FESEM is a versatile tool to be utilized in the biomedical sciences, especially in 3-dimensional reconstruction studies.

Dynamics of Attached Microbial Community on the River with Gravel Riverbed (자갈하상 하천에서 부착미생물군집의 거동)

  • Park, Jae-Young;Choi, I-Song;Oh, Jong-Min
    • Journal of Korea Water Resources Association
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    • v.38 no.3 s.152
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    • pp.235-244
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    • 2005
  • This study was carried out in Osan river to quantitatively investigate behavior of attacked microbial community (AMC) for enhancing self-purification process of river. We gained the results such as follows throughout long-term monitoring at in-situ river. The biomass of AMC had higher in the riffle than the almost stagnant pool and they were more developed in the riffle with high current velocity (HCV). Although the fast flowing current affects negatively to growth of the AMC during the early phase when the community gets attached to the benthic substrate, it was observed that it affected positively to their growth during the intermediate and later phase after the community is adapted to the substrate. When turbulence due to external pressure (storm or discharge of dam and reservoir) occurs, the degree of separation depends upon the flowing strength and the type of the external pressure. Since the community is not all separated, recovery is rather fast. Therefore, this study found that the degree of reduction of the pollutant by self-purification of the stream is depended upon the riverbed shape and the AMC contributes to self-purification positively or negatively in river. Therefore, the riverbed shape must be constructed in accordance with the characteristics of water quality in stream. Furthermore, the technique of installing the water channel structure appropriate for each section must be developed to maximize self-purification ability.

The Effects of Negative Pressure and Drain Spacing in the Horizontal Method for an Early Settlement of Dredged and Filled Grounds (해안준설매립토의 조기안정을 위한 수평배수공법에서 부압과 배수재 배치간격의 영향)

  • 김수삼;한상재;김병일;김정기
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.15 no.1
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    • pp.1-10
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    • 2003
  • In this paper, the laboratory test results with middle-sired ,soil box test in modeling the in-situ installing of horizontal drains are discussed the estimation of the optimum negative pressure. The test was carried out in the different vacuum pressure conditions together with the measurement for the settlement and volume change of drained water by the installed drains during the consolidation process. After the test, the water content was measured to both directions of lateral distance from the drain and depth of the soil, to find out the distribution of ground improvement and strength enhancement. From the analysis on the distribution of water content, the gradual application of vacuum pressure to higher level by pre-determined stages starting from low vacuum pressure is found to be effective and desirable. In the comparison of the degrees of consolidation with elapsed time, the calculated value by the prediction method based on the Barren's conventional theory showed a good agreement with the measured value. With this, It is positively considered that the applicability of the prediction method based on Barren's theory to the practical design of horizontal drains can be justified such as in the calculation of drain spacing and consolidation period.

Application of Slip-line Method to the Evaluation of Plastic Zone around a Circular Tunnel (원형터널 주변의 소성영역 평가를 위한 slip-line 해석법 활용)

  • Lee, Youn-Kyou
    • Tunnel and Underground Space
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    • v.32 no.5
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    • pp.312-326
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    • 2022
  • The generalized Hoek-Brown (GHB) criterion, which is recognized as one of the standard failure conditions for rock mass, is specialized for rock engineering applications and covers a wide range of rock mass conditions. Accordingly, many research efforts have been devoted to the incorporation of this criterion into the stability analysis of rock structures. In this study, the slip-line analysis method, which is a kind of elastoplastic analysis method, is combined with the GHB failure criterion to derive analytical equations that can easily calculate the plastic radius and stress distribution in the vicinity of the circular tunnel. In the process of derivation of related formulas, it is assumed that the behavior of rock mass after failure is perfectly plastic and the in-situ stress condition is hydrostatic. In the formulation, it is revealed that the plastic radius can be calculated analytically using the two respective tangential friction angles corresponding to the stress conditions at tunnel wall and elastic-plastic boundary. It is also shown that the plastic radius and stress distribution calculated using the derived analytical equations coincide with the results of Lee & Pietruszczak's numerical method published in 2008. In the latter part of this paper, the influence of the quality of the rock mass on the size of the plastic zone, the stress distribution, and the change of the tangential friction angle was investigated using the derived analytical equations.

Analysis on Surface Collapse of the Road NATM Tunnel through the Weathered Rock (풍화대를 통과하는 도로 NATM 터널의 천단부 함몰에 대한 연구)

  • Shin, Eun-Chul;Yoo, Jai-Sung
    • Journal of the Korean Geosynthetics Society
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    • v.15 no.2
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    • pp.55-64
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    • 2016
  • The construction of the road NATM tunnel, which undergoes the weathered zone of the mountain, was in process with the reinforcement methods such as the rock bolt, shotcrete depositing, and the multi step grout with large diameter steel pipe. The collapse from the ceiling, and on the ground surface area(sink hole), of which were measured to be 25m from the ground surface($V=12m(W){\times}14m(L){\times}5m(H)=840m^3$), as well as excessive displacements in the tunnel, had occurred. In order to execute the necessary reconstruction work, the causes of the surface collapses were inspected through the field investigation, in-situ tests, and numerical analysis. As a result, several proper solutions were suggested for both internal and external reinforcements for the tunnel. As a result of numerical analysis, the collapsed zone of the tunnel was reinforced up to 0.5D~1.0D laterally by the cement grouting on the ground surface, 0.5D longitudinally by the multi step grout with large diameter steel pipe in tunnel. With further reinforcement implemented by rebars in lining, the forward horizontal boring was executed to the rest of the tunnel to evaluate the overall status of the tunnel face. Appropriate reinforcement methods were provided if needed.

Application of Molecular Diagnostics Technology in the Development of a Companion Diagnostics for Malignant Solid Tumors (악성 고형암의 항암제 동반진단 기술에서 분자진단기술의 적용)

  • Kim, Jin-Hee
    • The Journal of the Korea Contents Association
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    • v.19 no.3
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    • pp.365-374
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    • 2019
  • Unlike benign tumors, malignant tumors are capable of metastasis, easy to relapse, poor survival, and low quality of life. In Korea, here is a tendency to treat the tumors collectively according to the General Principles of Cancer Chemotherapy(GPCC) of the Health Insurance Review & Assessment Service (HIRA). But recently, companion diagnostics(CDx) is recommended rather than unilateral medication because biomarker-based molecular diagnostics is possible to predict the drug response of patients before drug treatment. Not only domestic but also overseas Food and Drug Administratio (FDA) recommends the development of the CDx system at the stage of drug development to ensure the responsiveness and safety of medicines. In this study, I focused on the necessity of CDx development direction as well as CDx development status through literature review. Furthermore I also discussed CDx types according to the molecular diagnostic technology such as immunohistochemistry (IHC), polymerase chain reaction (PCR), in situ hybridization (ISH), and next-generation sequencing (NGS) not only in the approved CDx but also in the developing one by US FDA. And I suggested the technology issue of CDx development process such as a selection of molecular diagnostics at the time of release, a clear understanding of the CDx mechanism, and a convergence of drug with CDx development. The necessity of social insurance system also was proposed for CDx development.

Preliminary Evaluation of Domestic Applicability of Deep Borehole Disposal System (심부시추공 처분시스템의 국내적용 가능성 예비 평가)

  • Lee, Jongyoul;Lee, Minsoo;Choi, Heuijoo;Kim, Kyungsu;Cho, Dongkeun
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.16 no.4
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    • pp.491-505
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    • 2018
  • As an alternative to deep geological disposal technology, which is considered as a reference concept, the domestic applicability of deep borehole disposal technology for high level radioactive waste, including spent fuel, has been preliminarily evaluated. Usually, the environment of deep borehole disposal, at a depth of 3 to 5 km, has more stable geological and geo-hydrological conditions. For this purpose, the characteristics of rock distribution in the domestic area were analyzed and drilling and investigation technologies for deep boreholes with large diameter were evaluated. Based on the results of these analyses, design criteria and requirements for the deep borehole disposal system were reviewed, and preliminary reference concept for a deep borehole disposal system, including disposal container and sealing system meeting the criteria and requirements, was developed. Subsequently, various performance assessments, including thermal stability analysis of the system and simulation of the disposal process, were performed in a 3D graphic disposal environment. With these analysis results, the preliminary evaluation of the domestic applicability of the deep borehole disposal system was performed from various points of view. In summary, due to disposal depth and simplicity, the deep borehole disposal system should bring many safety and economic benefits. However, to reduce uncertainty and to obtain the assent of the regulatory authority, an in-situ demonstration of this technology should be carried out. The current results can be used as input to establish a national high-level radioactive waste management policy. In addition, they may be provided as basic information necessary for stakeholders interested in deep borehole disposal technology.

Estimation of Pile Ultimate Lateral Load Capacity in Sand Considering Lateral Stress Effect (응력상태를 고려한 사질토지반에 관입된 말뚝의 극한수평지지력 분석 및 평가)

  • Lee, Jun-Hwan;Paik, Kyu-Ho;Kim, Dae-Hong;Hwang, Sung-Wuk;Kim, Min-Kee
    • Journal of the Korean Geotechnical Society
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    • v.23 no.4
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    • pp.161-167
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    • 2007
  • In this study, ultimate lateral load capacity of piles is analyzed with consideration of lateral stress effect. Based on results obtained in this study, a method for the estimation of ultimate lateral load capacity is proposed. This makes it possible to more realistically estimate the ultimate lateral load capacity under various stress states caused by in-situ soil condition and pile installation process. Calibration chamber test results with various soil conditions were used in the analysis. From the test results, it was found that effect of the lateral stress was greater than that of the vertical stress on the ultimate lateral load capacity of piles. It was also found that, as the relative density increases, displacements required to reach the ultimate state increases, showing relative displacements of around 14% and 18-25% for $D_R$ : 55% and 86%, respectively. Based on results obtained in this study, a methodology for the estimation of ultimate lateral load capacity of piles using correction factors was proposed. Results from proposed method matched well measured results.