• 제목/요약/키워드: in situ method

검색결과 1,601건 처리시간 0.029초

장관내(腸管內) 칼슘흡수 실험방법에 관한 연구 (An Experimental Method of Calcium Absorption in Rat Small Intestine - in situ Closed Gut Segment Technique -)

  • 이연숙
    • 한국식품영양과학회지
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    • 제12권1호
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    • pp.41-45
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    • 1983
  • 본 연구는 식후(食後) 단시간내(短時間內)에 장내용물(腸內容物)이 존재(存在)하고 있는 장(腸)loop내(內)에 $^{45}Ca$을 직접 주입(注入)한 후, 장(腸)loop로부터의 $^{40}Ca$$^{45}Ca$의 소실량(消失量), 또는 문맥혈중(門脈血中) $^{45}Ca$의 출현량(出現量)에 의해 Ca흡수량을 측정 비교할 수 있는 in situ closed gut segment법(法)의 하나를 확립(確立)하고져 행하여 졌다. 성장기 흰쥐에게 casein식(食)을 급여하고 검토한 결과, in situ closed gut segment법(法)의 실험 최적조건(最適條件)은 ligation부위는 회장중앙부 15-20cm, 주입액(注入液)의 조성(組成)은 $0.2{\sim}0.5{\mu}Ci/5{\mu}gCa/0.5ml$, pH 7.0, $37^{\circ}C$, 장(腸)loop로부터 $^{40}Ca$$^{45}Ca$의 소실량(消失量) 측정(測定)은 $^{45}Ca$주입후(注入後) 30분간, 문맥혈중(門脈血中)의 $^{45}Ca$농도 측정은 15분간이었다. 이상의 실험조건에서 흰쥐에게 casein식(食)과 egg albumin식(食)을 급여하고 Ca흡수량을 비교한 결과, casein식(食)에 의해 2~5 배의 높은 Ca흡수효과를 보였으며, 문맥혈중 $^{45}Ca$농도로부터의 평가는 장(腸)loop로 부터의 평가보다 과소평가 되지만 비교연구에는 충분히 유효함을 보였다.

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Templated Formation of Silver Nanoparticles Using Amphiphilic Poly(epichlorohydrine-g-styrene) Film

  • Park, Jung-Tae;Koh, Joo-Hwan;Seo, Jin-Ah;Roh, Dong-Kyu;Kim, Jong-Hak
    • Macromolecular Research
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    • 제17권5호
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    • pp.301-306
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    • 2009
  • This work has demonstrated that a novel amphiphilic poly(epichlorohydrine)-graft-polystyrene (PECH-g-PS) copolymer at 34:66 wt% was synthesized via atom transfer radical polymerization (ATRP) of styrene using PECH as a macroinitiator. The structure of the graft copolymer was characterized by nuclear magnetic resonance ($^1H$ NMR) and FTIR spectroscopy, demonstrating that the "grafting from" method using ATRP was successful. The self-assembled graft copolymer was used as a template film for the in-situ growth of silver nanoparticles from $AgCF_3SO_3$ precursor under UV irradiation. The in situ formation of silver nanoparticles with 6-8 nm in average size in the solid state template film was confirmed by transmission electron microscopy (TEM), UV-visible spectroscopy and wide angle X-ray scattering (WAXS). Differential scanning calorimetry (DSC) also displayed the selective incorporation and the in situ formation of silver nanoparticles within the hydrophilic PECH domains, probably due to stronger interaction of the silvers with the ether oxygens of PECH backbone than that with hydrophobic PS side chains.

Modified Principal Component Analysis for In-situ Endpoint Detection of Dielectric Layers Etching Using Plasma Impedance Monitoring and Self Plasma Optical Emission Spectroscopy

  • Jang, Hae-Gyu;Choi, Sang-Hyuk;Chae, Hee-Yeop
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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    • pp.182-182
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    • 2012
  • Plasma etching is used in various semiconductor processing steps. In plasma etcher, optical- emission spectroscopy (OES) is widely used for in-situ endpoint detection. However, the sensitivity of OES is decreased if polymer is deposited on viewport or the proportion of exposed area on the wafer is too small. Because of these problems, the object is to investigate the suitability of using plasma impedance monitoring (PIM) and self plasma optical emission spectrocopy (SPOES) with statistical approach for in-situ endpoint detection. The endpoint was determined by impedance signal variation from I-V monitor (VI probe) and optical emission signal from SPOES. However, the signal variation at the endpoint is too weak to determine endpoint when $SiO_2$ and SiNx layers are etched by fluorocarbon on inductive coupled plasma (ICP) etcher, if the proportion of $SiO_2$ and SiNx area on Si wafer are small. Therefore, modified principal component analysis (mPCA) is applied to them for increasing sensitivity. For verifying this method, detected endpoint from impedance monitoring is compared with optical emission spectroscopy.

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벤치발파에서 파쇄도 예측을 위한 암반조건 분석 (Analysis of In-situ Rock Conditions for Fragmentation Prediction in Bench Blasting)

  • 최용근;이정인;이정상;김장순
    • 터널과지하공간
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    • 제14권5호
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    • pp.353-362
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    • 2004
  • 벤치발파에서 암석 파쇄도 예측은 생산계획을 수립하는 데 있어서 가장 중요한 요소 중의 하나이다. Kua-Ram 모델은 지금까지 제안된 암석 파쇄도 예측 모델 중 가장 우수한 것으로 평가받고 있으나, 이 모델의 평가항목을 구성하고 있는 절리조건, 암석강도, 밀도, 사용폭약의 성능과 저항선, 공간격 등의 요소들은 그 값을 선정하는 과정에서 주관적인 요소가 개입되거나 정의가 모호한 요소를 포함하고 있다. 이 연구에서는 Kuz-Ram 모델을 구성하는 여러 평가항목의 값을 선정하는 과정에서 주관적이거나 모호한 요소를 제거하는 방법에 대해 검토하였으며, 예측값을 현장조사 결과와 비교하여 적용성을 검토하였다. Kuz-Ram 모델은 비교적 정확한 예측결과를 보였으나, 현지암반 조건을 충분히 반영하지 못하여 개선이 필요한 것으로 판단되었다.

CFD 해석을 이용한 현장 열응답 시험의 출구온도 예측 (The Outlet Temperature Prediction of In-Situ Thermal Response Test using CFD Analysis)

  • 심용섭;이희상
    • 한국산학기술학회논문지
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    • 제17권1호
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    • pp.28-35
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    • 2016
  • 지열원 히트펌프 시스템에서 지중 열교환기 설계를 위해 수행하는 현장 열응답 시험은 많은 시간과 비용이 수반되기 때문에 조건 변화에 따른 출구온도 예측이 어려운 실정이다. 본 연구에서는 국내에서 주로 사용하는 수직형 지중 열교환기의 열전달 성능을 예측하기 위한 방안으로 3차원 CFD 해석 기법을 제안하고, 2개소의 현장 열응답 시험의 출구온도와 기울기 값을 비교하여 CFD 해석 방법의 신뢰성을 확인하였다. CFD 해석 결과, 2개소의 현장 열응답 시험의 출구온도는 $0.5^{\circ}C$ 이내에서 예측하였고, 기울기 값은 1.6% 이내에서 적절히 예측하였다. 이를 통해 CFD 해석 방법의 신뢰성을 확인하고, 2개소의 현장 열응답 시험의 유량 및 지중 유효 열전도도 조건을 각각 ${\pm}20%$ 변화시키면서 현장 열응답 시험의 출구온도를 예측하였다. 첫 번째 현장(Case 1)의 경우 유량 변화에 따라 $28.0^{\circ}C$(-20%)와 $29.6^{\circ}C$(+20%), 지중 유효 열전도도 변화에 따라 $29.6^{\circ}C$(-20%)와 $28.0^{\circ}C$(+20%)로 현장 열응답 시험의 출구온도를 예측하였으며, 두 번째 현장(Case 2)의 경우 유량 변화에 따라 $28.4^{\circ}C$(-20%)와 $29.8^{\circ}C$(+20%), 지중 유효 열전도도 변화에 따라 $29.7^{\circ}C$(-20%)와 $28.4^{\circ}C$(+20%)로 현장 열응답 시험의 출구온도를 각각 예측하였다.

Stabilization of Meles Delta soils using cement and lime mixtures

  • Onal, Okan;Sariavci, Cagrihan
    • Geomechanics and Engineering
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    • 제19권6호
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    • pp.543-554
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    • 2019
  • İzmir Bay reserves high amount of residual alluvial deposits generated by Meles River at its stream mouth. These carried sediments with high water content and low bearing capacity are unsuitable in terms of engineering purposes. In-situ soil stabilization with deep soil mixing method is considered to improve properties of soil in this location. This method is widely used especially over Scandinavia, Japan and North America. Basically, the method covers mixing appropriate binder into the soil to improve soil profile according to the engineering needs. For this purpose, soil samples were initially provided from the site, classification tests were performed and optimum ratios of lime and cement binders were determined. Following, specimens representing the in-situ soil conditions were prepared and cured to be able to determine their engineering properties. Unconfined compression tests and vane shear tests were applied to evaluate the stabilization performance of binders on samples with different curing periods. Scanning electron microscope was used to observe time-dependent bonding progress of binders in order to validate the results. Utilization of 4% lime and 4% cement mixture for the long-term performance and 8% lime and 8% cement mixture for short term performance were suggested for the stabilization of Meles Delta soils. Development of CSH and CAH in a gel form as well as CSH crystals were clearly observed on SEM images of treated specimens.

Aujeszky's disease virus 국내분리주 접종자돈의 병리발생에 관한 연구 II. 면역조직화학 및 in situ hybridization 기법을 이용한 항원과 핵산 검출 (Studies on the pathogenesis of Korean isolate of Aujeszky's disease virus in experimentally infected piglets II. Immunohistochemistry and detection of viral nucleic acids by in situ hybridization)

  • 조우영;조성환;박최규;김재훈;현방훈;윤용덕;권창희
    • 대한수의학회지
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    • 제36권4호
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    • pp.859-871
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    • 1996
  • This study was conducted to elucidate the distribution of Aujeszky's disease viral nucleic acids and antigens in the central nervous system (CNS) of piglets. The first Korean isolate of Aujeszky's disease virus(ADV) that isolated from naturally infected piglets in Yang San, was inoculated into 32 day old piglets with $10^{5.9}TCID_{50}/ml$ through intranasal or intramuscular route. These piglets were sacrificed at every 24hrs for 8 days. The immunohistochemistry (IHC) was conducted to detect the viral antigens in paraffin-embedded tissue sections using avidin-biotin-peroxidase complex (ABC) method. The viral nucleic acids were detected by in situ hybridization (ISH) using ADV specific DNA probe labeled with digoxigenin. The ADV antigens were detected in reticuloendothelial cells of spleen, lymph nodes and tonsil, alveolar walls, leptomeningeal vascular walls, inflammatory foci of each organ, and nerve cells. The viral nucleic acids were detected in the spinal trigeminal nucleus and its tracts of the pons and medulla oblongata by the ISH technique. The pathways of AD viruses in CNS were determined by IHC and ISH. In the intranasally inoculated group, the viruses in nasal mucosa moved to medulla oblongata and pons through the trigeminal nerve. In case of intramuscullarly inoculated group, viruses moved to brain via lymphoid organs or spinal nerves from sciatic nerves.

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In-Situ Observation of New Extra-Vascular Threadlike Structure of Mouse Using a Fluorescence Stereoscopic Microscope

  • Sung, Baeck-Kyoung;Lee, Ja-Woong;Lee, Byung-Cheon;Johng, Hyun-Min;Baik, Ku-Youn;Nam, Tae-Jeong;Park, Dae-Hun;Soh, Kyeong-Sun;Soh, Kwang-Sup
    • 대한약침학회지
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    • 제7권3호
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    • pp.73-76
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    • 2004
  • We report the new threadlike structures outside the blood vessels of mice. For this, we developed an in-situ searching method of the structure by vital staining with the dye of acridine orange and using a fluorescent stereomicroscope designed specifically for this purpose. We consider that the newly found threadlike structure might be rediscovery of the extra-vascular Bonghan duct which was reported in 1963 by Bonghan Kim.

Preparation and Properties of Crosslinkable Waterborne Polyurethanes Containing Aminoplast(I)

  • Kwon Ji-Yun;Kim Han-Do
    • Macromolecular Research
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    • 제14권3호
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    • pp.373-382
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    • 2006
  • A series of crosslinkable, waterborne polyurethanes (I-WBPUs) were prepared by in-situ polymerization using isophorone diisocyanate (IPDI)/poly(tetramethylene oxide) glycol (PTMG, $M_n$=2,000)/dimethylol propionic acid (DMPA)/ethylene diamine (EDA)/triethylamine (TEA)/aminoplast[hexakis(methoxymethyl)melamine (HMMM)] as a crosslinking agent. Typical crosslinkable, waterborne polyurethanes (B-WBPUs) blended from WBPU dispersion and aqueous HMMM solution was also prepared to compare with the I-WBPUs. The crosslinking reaction between WBPU and HMMM was verified using FTIR and XPS analysis. The effect of the HMMM contents on the dynamic mechanical thermal, thermal, mechanical, and adhesion properties of the I-WBPU and B-WBPU films were investigated. The storage modulus(E'), glass transition temperatures of the soft segment ($T_{gs}$) and the amorphous regions of higher order ($T_{gh}$), melting temperature ($T_m$), integral procedural decomposition temperature (IPDT), residual weight, $T_{10%}$ and $T_{50%}$ (the temperature where 10 and 50% weight loss occurred), tensile strength, initial modulus, hardness, and adhesive strength of both I-WBPU and B-WBPU systems increased with increasing HMMM content. However, these properties of the I-WBPU system were higher than those of the B-WBPU system at the same HMMM content. These results confirmed the in-situ polymerization used in this study to be a more effective method to improve the properties of the WBPU materials compared to the simple blending process.

도로기초에서 교통 및 환경하중에 의한 비선형 현장응력 평가 (Evaluation of Traffic Load and Moisture-Induced Nonlinear In-situ Stress on Pavement Foundation Layers)

  • 박성완;황규영;정문경;서영국
    • 한국지반공학회논문집
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    • 제25권7호
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    • pp.47-54
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    • 2009
  • 도로하부에 대한 현장에서의 역학적인 거동에 대한 이해는 교통기반시스템의 장기공용성을 예측하는데 매우 중요하다. 이러한 현장거동에서 지반재료에 대한 회복변형 거동 정량화는 교통하중과 환경조건을 고려한 역학적인 해석이 필수적이다. 따라서 본 논문에서는 한국도로공사 시험도로에서 현장자료의 계측과 분석을 통하여 선택된 도로하부 입상재료에 대한 구성방정식을 활용한 비선형 재료거동과 응력을 예측 분석하고 이를 현장자료와 비교하고 검증하였다.