• 한국군사과학기술학회지
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• 제8권3호
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• pp.83-91
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• 2005

Pyrolysis-mass spectrometry has been used to characterize the 17 biological materials including bacteria and proteins. In this study, an in situ thermal-hydrolysis methylation(THM) procedure using tetramethylammonium hydroxide(TMAH) was employed. The biological materials are ionized using chemical ionization(CI) method with ethanol by ion trap mass spectrometer(ITMS), which attached with our own made pyrolyzer module, and then their pyrolysis mass spectra were obtained. The major distinct characteristic peaks were selected from all the range of mass spectra, and analyzed using principal component analysis(PCA) method to assess the classification/identification possibility of biological materials.

• 터널과지하공간
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• 제5권3호
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• pp.251-265
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• 1995

One of the most important matters in stress measurement by hydraulic fracturing technique is the determination of the breakdown pressure, reopening pressure, and shut-in pressure, since these values are the basic input data for the calculation of the in-situ stress. The control of the fracture propagation is also important when the hydraulic fracturing technique is applied to the development of groundwater system, geothermal energy, oil, and natural gas. In this study, a laboratory scale hydraulic fracturing device was built and a series of model tests were conducted with cube blocks of Machon gabbro. A new method called 'flatjack method' was adopted to determine shut-in pressure. The initial stress calculated from the shut-in pressure measured by flatjack method showed much higher accuracy than the stress determined by the conventional method. The dependency of the direction of fracture propagation on the state of the initial stresses was measured by introducin g artificial slots in the borehole made by water jet system. Numerical modeling by BEM was also performed to simulate the fracture propagation process. Both results form numerical and laboratory tests showed good agreement. From this study which provides the extensive results on the determination of shut-in pressure and the control of fracture propagation which are the critical issue in the recent hydraulic fracturing, it is conclued that in-situ stress measurement and the control of fracture propagation could be achived more accurately.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2009년도 춘계 학술발표회
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• pp.23-29
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• 2009

A sand-cone method is commonly used to determine the density of the compacted soils. This method uses a calibration container to determine the bulk-density of the sand for use in the test. The density of the test or compacted soil is computed on the assumption that the calibration container has approximately the same size or volume and allows the sand to fall approximately the same height as a test hole in the field. However, in most cases the size or shape of test hole is not exactly the same as the calibration container. There is certain discrepancy between sand particle settlement or arrangement in the laboratory calibration and in the field testing, which may cause an erroneous determination of in-situ density. The sand filling process is simulated in the laboratory and its effect on the determination of density is investigated. Artificially-made holes with different heights and bottom shapes are prepared to simulate various shapes of the test hole in the field. The sands with different gradations are used in the testing to examine how sand grain size influences the determination of density in the field.

• Molecules and Cells
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• 제24권1호
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• pp.76-82
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• 2007

By combining in situ hybridization (ISH) and immunocytochemistry (IC), microscopic topological localization of mRNAs and proteins can be determined. Although this technique can be applied to a variety of tissues, it is particularly important for use on neuronal cells which are morphologically complex and in which specific mRNAs and proteins are located in distinct subcellular domains such as dendrites and dendritic spines. One common technical problem for combined ISH and IC is that the signal for immunocytochemical localization of proteins often becomes much weaker after conducting ISH. In this manuscript, we report a simplified but robust protocol that allows immunocytochemical localization of proteins after ISH. In this protocol, we fix cultured cortical or hippocampal neurons with 4% paraformaldehyde (PFA), rinse briefly in PBS, and then further fix the cells with $-20^{\circ}C$ methanol. Our method has several major advantages over previously described ones in that (1) it is simple, as it is just consecutive routine fixation procedures, (2) it does not require any special alteration to the fixation procedures such as changes in salt concentration, and (3) it can be used with antibodies that are compatible with either methanol (MeOH-) or PFA-fixed target proteins. To our best knowledge, we are the first to employ this fixation method for fluorescence ISH + IC.

• 한국터널지하공간학회 논문집
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• 제9권1호
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• pp.49-62
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• 2007

본 연구에서는 사질토 지반에 설치된 수직갱에 작용하는 토압에 대한 기존 제안식을 수정하였다. 이를 검증하기 위하여 깊이에 관계없이 동일한 반경방향 변위를 일으킬 수 있는 실내 모형시험장치를 개발하였으며, 벽면마찰각과 상대 밀도를 변수로 시험하였다. 실내 모형시험 결과는 수정식에서 접선방향 토압계수(수직응력에 대한 접선방향 응력 비)인 $\lambda$값을 $\lambda=1-sin\phi$와$\lambda=1$을 사용하였을 경우의 토압 사이에 분포하였다. 다층지반에 설치된 원형수직갱 배면지반의 파괴면을 가정하고 수정식을 적용하여 다층지반에 설치된 원형수직갱에 작용하는 토압 산정방법을 제안하였다. 시공현장의 3개의 수직갱으로부터 계측 데이터를 획득하였으며 이를 토압으로 환산하여 제안된 방법을 검증하였다. 계측 데이터로부터 환산된 대부분의 환산 토압은 제안된 방법의 토압과 잘 일치하였다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2005년도 Proceedings of ISRS 2005
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• pp.671-674
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• 2005

Atmospheric correction over the ocean part is more important than that over the land because the signal from the ocean is very small about one tenth of that reflected from land. In this study, the Spectral Shape Matching Method (SSMM) developed by Ahn and Shanmugam (2004) is evaluated using Landsat imagery acquired over the highly turbid Saemangeum Coastal Area. The result of SSMM is compared with COST model developed by Chavez (1991 and 1997). In principle, SSMM is simple and easy to implement on any satellite imagery, relying on both field and image properties. To assess the potential use of these methods, several field campaigns were conducted in the Saemangeum coastal area corresponding with Landsat-7 satellite's overpass on 29 May 2005. In-situ data collected from the coastal waters of Saemangeum using optical instruments (ASD field spectroradiometer) consists of ChI, Ap, SS, aooM, F(d). In order to perform SSMM, we use the in-situ water-leaving radiance spectra from clear oceanic waters to estimate the the path radiance from total signal recorded at the top of the atmosphere (TOA), due to the reason that the shape of clear water-leaving radiance spectra is nearly stable than turbid water-leaving radiance spectra. The retrieved water-leaving radiance after subtraction of path signal from TOA signal in this way is compared with that estimated by COST model. The result shows that SSMM enabled retrieval of water-leaving radiance spectra that are consistent with in-situ data obtained from Saemangeum coastal waters. The COST model yielded significantly high errors in these areas.

• 설비공학논문집
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• 제18권10호
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• pp.783-790
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• 2006

A one-dimensional numerical model coupled with parameter estimation is used to predict the effective thermal conductivities of soil formations and borehole resistances from in situ field test data. In this application a new method of using initial ignoring time (IIT) obtained from error estimation is tried and turned out to be successful in determining soil thermal conductivities. This method is used for single-U and double-U borehole system. The results of this method are compared and agreed well with those of existing software (GPM) in the analysis of single-U borehole data. In the analysis of double-U borehole data this method seems to be better in predicting soil and borehole properties.

• 대한수의학회지
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• 제39권1호
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• pp.148-158
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• 1999

The purpose of this study was to establish a rapid, reliable diagnostic method detecting Encephalomyocarditis virus(EMCV) RNA in formalin-fixed, paraffin-embedded tissues of EMCV naturally infected pigs by cDNA probe of EMC $K_3$, the EMCV strain isolated from Korea. Using a biotin-labelled nick translated probe for the cDNA marker. We made up for some defects of radiolabeled method. In sits hybridization(ISH) technique, differently from the other nucleic acid hybridization methods, is able to detect the virus genome specifically in the state of the intact shapes of cells and/or tissues. We succeeded in performing the experiment to detect the EMCV within 1~2 hours using the $MicroProbe^{TM}$ capaillary action system. In this study, we observed highly specific positive signals of red color by staining the paraffin-embedded tissue sections of naturally EMCV-infected pig organs or tissues, including brain, heart, kidney and lacrimal gland with the Fast Red TR salt/Naphtol phosphate chromogen. The results suggested that this ISH method is considered as a highly sensitive and reliable tool for molecular biologic diagnosis of the EMC viral disease.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2009년도 춘계 학술발표회
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• pp.888-895
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• 2009

Compaction is a process of increasing soil density using physical energy. It is intended to improve the strength and stiffness of soil. In embankment, degree of compaction affects the construction time, money, also method of soil improvement. In large scale embankment project, difficulties of embankment should change due to uncertainty of settlement. So it is very important to predict the final settlement and factor of safety induced by embankment. In many construction site, there are primarily design of high embankment using in-situ soil. Therefore numerical analyses are necessary for valid evaluation of the settlement prediction. But due to the construction cost and schedule, there were lacking in properties of soil and also limited number of in-situ test were performed. So we proposed the method that can easily estimate the proper soil parameters and suggest the proper method of numerical analysis. From this, two-dimensional finite-difference numerical analysis was conducted to investigate the settlement and factor of safety induced by embankment with various case of compaction rate and embankment height.

• 한국환경복원기술학회지
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• 제9권1호
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• pp.100-111
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• 2006

This study was carried out to increase the efficiency of site investigation through the evaluation of the applicable methods of investigation for cutting slope design. In an effort to find out the applicability of the method employed during the slope investigation, some tests were carried out on 6 subjects i.e location of weak zones, characteristics of discontinuities, distribution of strata, in situ tests for geo-technical properties, laboratory test and estimation of weathering. The method was highly applicable and produced expected results during the test of weak zones, discontinuities and distribution of strata. In order to apply the method to in- situ test for geo-technical properties and laboratory test on soil and rock slope, a statistic analysis of the existing data were required in advance. However its design applicability on rock slope was good although actual cases of application were not many due to limitation of the cases for investigation. The method was frequently referenced but not actually applied for anti seismic design test and estimation of weathering.