• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.633-634
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• 2001

Porcine pancrease lipase를 aldehyde-silica에 in situ 상태에서 immobilization 할때의 최적 조건에 대하여 연구하였다. Feed 용액의 조성비율, 양, pH, 온도, 농도, 유속에 대하여 최적화하였으며 lipase의 activity를 측정 비교하였다.

• 한국환경성돌연변이발암원학회지
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• 제17권1호
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• pp.12-16
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• 1997

Chromosome rearrangement induced by bleomycin were identified by fluorescence in situ hybridization with probe for chromosome 4. The frequency of color junctions, translocations, dicentric and acenttic fragments increased with bleomycin dose. Different types of balanced translocation and dicentric were scored and compared. The frequency of cells exhibiting multiple aberration was higher compared to that of cells exposed to Gamma radiation suggesting that effect of bleomycin might be similar to that of high LET radiation.

• 셀메드
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• 제11권1호
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• pp.6.1-6.7
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• 2021

In situ gelling devices, as they enter the body, are dosage forms in the shape of the sol but turn into gel types under physiological circumstances. Transition from sol to gel is contingent on one or a mixture of diverse stimuli, such as transition of pH control of temperature, irradiation by UV, by the occurrence of certain ions or molecules. Such characteristic features may be commonly employed in drug delivery systems for the production of bioactive molecules for continuous delivery vehicles. The technique of in situ gelling has been shown to be impactful in enhancing the potency of local or systemic drugs supplied by non-parenteral pathways, increasing their period of residence at the absorption site. Formulation efficacy is further improved with the use of mucoadhesive agents or the use of polymers with both in situ gelling properties and the ability to bind with the mucosa/mucus. The most popular and common approach in recent years has provided by the use of polymers with different in situ gelation mechanisms for synergistic action between polymers in the same formulation. In situ gelling medicine systems in recent decades have received considerable interest. Until administration, it is in a sol-zone and is able to form gels in response to various endogenous factors, for e.g elevated temperature, pH changes and ions. Such systems can be used in various ways for local or systemic supply of drugs and successfully also as vehicles for drug-induced nano- and micro-particles. In this review we will discuss about various aspects about use of these in situ gels as novel drug delivery systems.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권6호
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• pp.465-470
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• 2004

Optimal conditions for the in situ immobilization of lipase in aldehyde-silica packed columns, via reductive amination, were investigated. A reactant mixture, containing lipase and sodium borohydride (NaCBH), was recirculated through an aldehyde-silica packed column, such that the covalent bonding of the lipase, via amination between the amine group of the enzyme and the aldehyde terminal of the silica, and the reduction of the resulting imine group by NaCBH, could occur inside the bed, in situ. Mobile phase conditions in the ranges of pH $7.0{\~}7.8$, temperatures between $22{\~}28^{circ}C$ and flow rates from $0.8{\~}1.5\;BV/min$ were found to be optimal for the in situ immobilization, which routinely resulted in an immobilization of more than 70 mg­lipase/g-silica. Also, the optimal ratio and concentration for feed reactants in the in situ immobilization: mass ratio [NaCBH]/[lipase] of 0.3, at NaCBH and lipase concentrations of 0.75 and 2.5 g/L, respectively, were found to display the best immobilization characteristics for concentrations of up to 80 mg-lipase/g-silica, which was more than a 2-fold increase in immobilization compared to that obtained by batch immobilization. For tributyrin hydrolysis, the in situ immobilized lipase displayed lower activity per unit mass of enzyme than the batch-immobilized or free lipase, while allowing more than a $45\%$ increase in lipase activity per unit mass of silica compared to batch immobilization, because the quantity of the immobilization on silica was aug­mented by the in situ immobilization methodology used in this study.

• 한국지반공학회논문집
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• 제18권2호
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• pp.65-74
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• 2002

지중응력의 측정은 터널 및 지하구조물의 변위와 응력분포를 예측하는데 중요하다. 본 연구에서는 남부 한반도 지역의 지중응력 분포를 음력해방법과 수압파쇄법으로 측정된 새로운 380개의 자료를 통하여 재평가와 이를 고려한 삼차원 터널해석을 실시하였다. 지중응력분포의 결과에 의하면 변성암과 화강암지역의 수평응력분포는 퇴적암과 화산암의 분포에 비하여 불규칙한 경향을 보이고 있다. 화산암 지역에서는 수직응력에 대한 수평음력의 비(측압계수, K)는 150m 깊이 이상 심도에서 1보다 작다. 또한, 국내에서는 처음으로 측정된 지중응력에 대한 삼차원 방향과 크기를 동시에 그림으로 나타내었다. 삼차원 터널 해석에 의하면, 터널 주변의 변위에 대한 방향과 크기는 최대수평음력과 최소수평응력의 차이에 의하여 주로 영향을 받고 있다.

• 한국초지조사료학회지
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• 제34권1호
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• pp.60-67
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• 2014

본 연구는 조사료의 반추위 발효가 진행됨에 따른 볏짚표면에 부착된 섬유소 분해 박테리아의 군집변화와 섬유소 소화율을 비교 관측하기 위하여 볏짚의 in situ 반추 발효를 실시하였다. 그리고 부착 박테리아의 군집 변화를 측정하기 위하여 RT-PCR 기법을 이용하여 F. succinogenes. R. albus와 R. flavefaciens의 군집을 모니터링 하였다. 본 연구를 수행하기 위하여 in situ 볏짚 발효를 0. 2, 4, 8, 12, 24시간 실시하였을 때 반추위내 볏짚의 in situ 분해는 발효 시간이 진행됨에 따라 가속화되어 발효 8~12시간 사이에 최고 분해 속도를 나타내었으나, F. succinogenes, R. flavefaciens과 R. albus는 모두 발효 0~1시간 사이에 볏짚 표면에 부착이 80% 이상 완료되어 이후 발효가 계속 진행되는 동안 일정 수준의 군락을 유지하는 것이 발견되었다. 그리고 반추위내 유입된 조사료의 표면에 초기 부착과정을 관찰하기 위하여 0, 5, 10, 30 및 60분 간격으로 볏짚의 in situ 샘플을 채취하여 조사하였을 때 F. succinogenes, R. flavefaciens 및 R. albus의 군락 모두 볏짚이 반추위 유입 후 5분 내에 상당량의 수가 부착함을 발견하였다. 또한 조사료의 반추위 발효 용이성에 따른 섬유소 분해 박테리아의 부착 정도를 관찰하기 위하여 0, 2, 4 및 8% NaOH를 처리한 볏짚을 12 및 24시간 in situ 배양 볏짚의 소화율과 부착 박테리아의 군집 변화를 관측하였을 때, 볏짚의 NaOH 처리 농도가 높아짐에 따라 in situ 소화율이 증가하였으며, 동시에 부착된 박테리아 군집의 증가 경향이 F. succinogenes, R. flavefaciens 및 R. albus의 3균주 모두 배양 12시간에 나타났으나 배양 24시간에서는 각기 다른 양상을 나타냈다. 따라서 본 연구결과는 반추위내 섬유소 발효과정에서 섬유소 분해 박테리아의 부착은 조사료의 반추위 유입 초기에 반드시 이루어지고, 발효 시간이 진행됨에 따라 조사료 표면에 안정된 군락을 형성하며, 섬유소 분해가 가속화된다는 사실을 보여 주었다.

• KSBB Journal
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• 제31권4호
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• pp.284-290
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• 2016

In this research, recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) was produced by transgenic rice cells. RAmy3D promoter was used for overcome the limitation of low expression level in transgenic plant cells, and the secretion of target protein was accomplished by signal peptide. However, the RAmy3D promoter system which can be induced only by sugar starvation causes the decrease of cell viability. As a result, cell death promotes the release of protease which degrades the target proteins. The protein stability and productivity can be significantly influenced by proteolysis activity. Therefore, development of new strategies are necessary for the in situ recovery of target proteins from cell culture media. In this study, in situ recovery was performed by various strategies. Direct addition of Protein A resin with nylon bag leads to cell death by increased shear stress and decrease in production of hCTLA4Ig by protease. Medium exchange through modified flask could recover hCTLA4Ig with high cell viability and low protease activity, on the other hand, the productivity was lower than that of control. When in situ recovery was conducted at day 7 after induction in air-lift bioreactor, 1.94-fold of hCTLA4Ig could be recovered compared to control culture without in situ recovery. Consequently, in situ recovery of hCTLA4Ig from transgenic rice cell culture could enhance productivity significantly and prevent degradation of target proteins effectively.

• Ocean and Polar Research
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• 제35권4호
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• pp.395-405
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• 2013

The aim of this study is to suggest an optimal survey method for coastal habitat monitoring around Weno Island in Chuuk Atoll, Federated States of Micronesia (FSM). This study was carried out to compare and analyze differences between in situ survey (PHOTS) and high spatial satellite imagery (Worldview-2) with regard to the coastal habitat distribution patterns of Weno Island. The in situ field data showed the following coverage of habitat types: sand 42.4%, seagrass 26.1%, algae 14.9%, rubble 8.9%, hard coral 3.5%, soft coral 2.6%, dead coral 1.5%, others 0.1%. The satellite imagery showed the following coverage of habitat types: sand 26.5%, seagrass 23.3%, sand + seagrass 12.3%, coral 18.1%, rubble 19.0%, rock 0.8% (Accuracy 65.2%). According to the visual interpretation of the habitat map by in situ survey, seagrass, sand, coral and rubble distribution were misaligned compared with the satellite imagery. While, the satellite imagery appear to be a plausible results to identify habitat types, it could not classify habitat types under one pixel in images, which in turn overestimated coral and rubble coverage, underestimated algae and sand. The differences appear to arise primarily because of habitat classification scheme, sampling scale and remote sensing reflectance. The implication of these results is that satellite imagery analysis needs to incorporate in situ survey data to accurately identify habitat. We suggest that satellite imagery must correspond with in situ survey in habitat classification and sampling scale. Subsequently habitat sub-segmentation based on the in situ survey data should be applied to satellite imagery.

• 미생물학회지
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• 제23권1호
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• pp.49-55
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• 1985

The survival of Escherichia coli in river water was comparatively studied by laboratory and in sity study methods. The survival by two methods was evaluated as a function of E. coli strain, indigeneous predator, level of water pollution, and water temperature in different season. The survival rate of E. coli examined by laboratory method was lower than that by in situ method. That was found to be due to the fact that higher number of predator was maintained in labortory study than in in situ study. The survival rates of E. coli in gradually polluted river waters could be differentiated by in situ study, but not by laboratory study. Therefore, an in situ method rather than labortory method was thought to be a choice of study method for the survival of E. coli in river waters.

• 자원환경지질
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• 제56권2호
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• pp.115-123
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• 2023

본 연구는 Drost et al. (2018)이 제안한 LA-ICP-MS를 이용한 새로운 'image-mapping' in-situ U-Pb 연대측정법을 소개하고, 국내 전기고생대 조선누층군 시료를 대상으로 처음 적용하여 결정한 절대연대 결과 사례를 통해 이 방법의 특성과 활용성을 알리고자 한다. 탄산염광물을 대상으로 하는 'image-mapping' in-situ U-Pb 연대측정법은 미세조직 관찰을 기초로 미소영역의 'image-mapping' 법을 적용하여, 기존의 점 분석(spot analysis) in-situ 분석법과는 달리, 신뢰성 높은 절대연대를 결정할 수 있을 뿐 아니라, 생성 이후 일어난 복수의 지질학적 '사건' 절대연대도 결정할 수 있다. 막골층과 대기층 탄산염광물로부터 퇴적연대와 함께 복수의 퇴적 이후의 연대를 결정한 적용 사례에서 이를 확인하였다. 따라서 이 같은 'image-mapping' in-situ U-Pb 연대측정법이 전 지질시대에 걸쳐 다양한 지질학적 환경에서 존재하는 다양한 형태의 탄산염광물 절대연대 결정에 적용된다면, 새로운 지질학적 연대정보 확보가 가능할 것이다.