• 제목/요약/키워드: in Situ

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Epstein-Barr 바이러스 인사이투 보결합 시행시 양성대조표지로서의 버키트 림프종 세포주 (CCL85 EB-3)의 응용 (Application of Epstein-Barr Virus Cell Lines (CCL85 EB-3) in Performing the EBER mRNA In Situ Hybridization as a Positive Control)

  • 김성숙;한운섭;서주영;허주령
    • 대한세포병리학회지
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    • 제7권1호
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    • pp.38-43
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    • 1996

  • Epstein-Barr virus(EBV) is associated with a wide spectrum of benign and malignant disorders including leukoplakia, Hodgkln's lymphoma, central nervous system lymphoma, peripheral T cell lymphoma and nasopharyngeal undifferentiated carcinoma. There are several distinctive aspects of biology of the virus that are important in investigation of virus in clinical specimens. The abundant expression of the EBER mRNA transcripts makes possible the sensitive detection of latent expression in EBV-associated tumors. Although there has been a dramatic increased interest in the direct characterization of EBV in clinical specimens, there have been few studios about the effective and reliable positive controls in performing in situ hybridization technique for EBV, especially on paraffin-em bedded tissue. We applied Burkitts lymphoma ceil line as positive control in EBV in situ hydridization using Oncor Kit. The cell block of Burkitt lymphoma cell line(CCL85 EB-3) showed strong and specific positivity for EBER in situ in nuclei of EBV infected cells.

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원전 고온 1차수 환경에서 응력부식균열의 실시간 마이크로 스케일 관찰 방법 개발 (Development of Method for In-situ Micro-Scale Observation of Stress Corrosion Cracking in High-Temperature Primary Water Environment)

  • 신정호;이종연;김성우
    • Corrosion Science and Technology
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    • 제22권4호
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    • pp.265-272
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    • 2023

  • The aim of this study was to develop a new in-situ observation method and instrument in micro-scale to investigate the mechanism of stress corrosion cracking (SCC) initiation of Ni-base alloys in a high temperature water environment of pressurized water reactors (PWRs). A laser confocal microscope (LCM), an autoclave with diamond window view port, and a slow strain-rate tester with primary water circulation loop system were components of the instrument. Diamond window, one of the core components of the instrument, was selected based on its optical, chemical, and mechanical properties. LCM was used to observe the specimen in micro-scale, considering the experimental condition of a high-temperature primary water environment. Using in-situ method and instrument, it is possible to observe oxidation and deformation of specimen surface in micro-scale through the diamond window in a high-temperature primary water in real-time. The in-situ method and instrument developed in this work can be utilized to investigate effects of various factors on SCC initiation in a high-temperature water environment.

  • https://doi.org/10.14773/cst.2023.22.4.265 인용 PDF