• 제목/요약/키워드: immunology

검색결과 1,688건 처리시간 0.049초

${\alpha}$-Mangostin Reduced ER Stress-mediated Tumor Growth through Autophagy Activation

  • Kim, Sung-Jin;Hong, Eun-Hye;Lee, Bo-Ra;Park, Moon-Ho;Kim, Ji-Won;Pyun, A-Rim;Kim, Yeon-Jeong;Chang, Sun-Young;Chin, Young-Won;Ko, Hyun-Jeong
    • IMMUNE NETWORK
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    • 제12권6호
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    • pp.253-260
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    • 2012

  • ${\alpha}$-Mangostin is a xanthon derivative contained in the fruit hull of mangosteen (Garcinia mangostana L.), and the administration of ${\alpha}$-Mangostin inhibited the growth of transplanted colon cancer, Her/CT26 cells which expressed Her-2/neu as tumor antigen. Although ${\alpha}$-Mangostin was reported to have inhibitory activity against sarco/endoplasmic reticulum $Ca^{2+}$ ATPase like thapsigargin, it showed different activity for autophagy regulation. In the current study, we found that ${\alpha}$-Mangostin induced autophagy activation in mouse intestinal epithelial cells, as GFP-LC3 transgenic mice were orally administered with 20 mg/kg of ${\alpha}$-Mangostin daily for three days. However, the activation of autophagy by ${\alpha}$-Mangostin did not significantly increase OVA-specific T cell proliferation. As we assessed ER stress by using XBP-1 reporter system and phosphorylation of $eIF2{\alpha}$, thapsigargin-induced ER stress was significantly reduced by ${\alpha}$-Mangostin. However, coadministration of thapsigargin with ${\alpha}$-Mangostin completely blocked the antitumor activity of ${\alpha}$-Mangostin, suggesting ER stress with autophagy blockade accelerated tumor growth in mouse colon cancer model. Thus the antitumor activity of ${\alpha}$-Mangostin can be ascribable to the autophagy activation rather than ER stress induction.

  • https://doi.org/10.4110/in.2012.12.6.253 인용 PDF KSCI

An Anti-Cancer Drug Candidate CYC116 Suppresses Type I Hypersensitive Immune Responses through the Inhibition of Fyn Kinase in Mast Cells

  • Park, Young Hwan;Kim, Hyun Woo;Kim, Hyuk Soon;Nam, Seung Taek;Lee, Dajeong;Lee, Min Bum;Min, Keun Young;Koo, Jimo;Kim, Su Jeong;Kim, Young Mi;Kim, Hyung Sik;Choi, Wahn Soo
    • Biomolecules & Therapeutics
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    • 제27권3호
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    • pp.311-317
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    • 2019

  • Mast cells are the most prominent effector cells of Type 1 hypersensitivity immune responses. CYC116 [4-(2-amino-4-methyl-1,3-thiazol-5-yl)-N-[4-(morpholin-4-yl)phenyl] pyrimidin-2-amine] is under development to be used as an anti-cancer drug, but the inhibitory effects of CYC116 on the activation of mast cells and related allergy diseases have not reported as of yet. In this study, we demonstrated, for the first time, that CYC116 inhibited the degranulation of mast cells by antigen stimulation ($IC_{50}$, ${\sim}1.42{\mu}M$). CYC116 also inhibited the secretion of pro-inflammatory cytokines including TNF-${\alpha}$ ($IC_{50}$, ${\sim}1.10{\mu}M$), and IL-6 ($IC_{50}$, ${\sim}1.24{\mu}M$). CYC116 inhibited the mast cell-mediated allergic responses, passive cutaneous anaphylaxis (ED50, ~22.5 mg/kg), and passive systemic anaphylaxis in a dose-dependent manner in laboratory experiments performed on mice. Specifically, CYC116 inhibited the activity of Fyn in mast cells and inhibited the activation of Syk and Syk-dependent signaling proteins including LAT, $PLC{\gamma}$, Akt, and MAP kinases. Our results suggest that CYC116 could be used as an alternative therapeutic medication for mast cell-mediated allergic disorders, such as atopic dermatitis and allergic rhinitis.

  • https://doi.org/10.4062/biomolther.2018.148 인용 PDF KSCI

The protective role of the polysaccharide Ginsan against Staphylococcus aureus : induction of NO and reduction of poinflammatory cytokines

  • Ahn, Ji-Yeon;Choi, In-Soo;Song, Jie-Young;Shim, Ji-Young;Jung, In-Seong;Yun, Yeon-Sook
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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    • pp.208.2-209
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    • 2003

  • Ginsan. a new polysaccharide isolated from Panax ginseng, has been previously reported as a good immunomodulator. In this study, we investigated the protective effect of Ginsan against a lethal sepsis induced by Staphylococcus aureus infection. The survival rate of mice treated with Ginsan 24 h prior to S. aureus infection was 80％ whereas PBS-treated mice showed 20％ of survival in the same infection. (omitted)

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