• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.591-599
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• 2008

A murine model immunized by systemic and mucosal delivery of plasmid DNA vaccine expressing glycoprotein B (pCIgB) of pseudorabies virus (PrV) was used to evaluate both the nature of the induced immunity and protection against a virulent virus. With regard to systemic delivery, the intramuscular (i.m.) immunization with pCIgB induced strong PrV-specific IgG responses in serum but was inefficient in generating a mucosal IgA response. Mucosal delivery through intranasal (i.n.) immunization of pCIgB induced both systemic and mucosal immunity at the distal mucosal site. However, the levels of systemic immunity induced by i.n. immunization were less than those induced by i.m. immunization. Moreover, i.n. genetic transfer of pCIgB appeared to induce Th2-biased immunity compared with systemic delivery, as judged by the ratio of PrV-specific IgG isotypes and Th1- and Th2-type cytokines produced by stimulated T cells. Moreover, the immunity induced by i.n. immunization did not provide effective protection against i.n. challenge of a virulent PrV strain, whereas i.m. immunization produced resistance to viral infection. Therefore, although i.n. immunization was a useful route for inducing mucosal immunity at the virus entry site, i.n. immunization did not provide effective protection against the lethal infection of PrV.

• IMMUNE NETWORK
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• 제9권1호
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• pp.8-11
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• 2009

There is mounting evidence that autophagy is involved in diverse physiological and pathological processes that have immense relevance in human development, diseases and aging. Immunity and inflammation are not exceptions. Here, the role of autophagy in the control of immune processes particularly that related to cell death and inflammation is discussed.

• 해양환경안전학회지
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• 제26권2호
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• pp.156-162
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• 2020

20세기 초부터 비상업용 정부선박의 법적 지위에 대한 검토와 법제화가 이루어졌다. 특히 전통적 국제법상의 절대적 주권면제이론에 대한 비판이 발생하고, 이에 대한 대체 이론으로 제한적 주권면제이론이 등장하면서 주권면제이론은 더욱 구체화 되었다. 국제관습법 및 국제해양법에서는 군함과 함께 비상업용 정부선박에 대한 주권면제를 규정하고 있다. 이러한 주권면제와 관련하여 고려되어야 하는 것이 우리나라 국립대학의 실습선이다. 우리나라 국립대학은 고급해기사 양성 및 어업 실습 등의 교육목적으로 실습선 및 조사선을 건조하여 운용하고 있다. 이러한 실습선은 국내 관할수역 뿐만 아니라 타국의 관할수역 및 공해까지를 활동범위로 하고 있을 뿐만 아니라 타국의 항구에도 입항하고 있다. 따라서 국제법상 국립대학 실습선의 법적지위를 명확하게 하는 것은 국제 항행에 있어 중요한 요소 중에 하나라고 할 수 있을 것이다. 그러나 실습선이 어떠한 법적 지위를 누리느냐에 대해서는 단순히 답할 수 없는 요소들이 다수 존재한다. 이에 본 논문은 국제법상 비상업용 정부선박의 개념 및 의의에 대해 살펴보고, 국립대학 실습선이 이와 일치하는지에 대해 검토하였다. 또한 전통 국제법상의 주권면제 범위와 현행 국제협약상의 주권면제에 대한 규정을 살펴봄으로써 우리나라 국립대학 실습선이 해양에서 누릴 수 있는 주권면제의 범위에 대해 살펴보았다.

• IMMUNE NETWORK
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• 제6권4호
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• pp.185-191
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• 2006

Background: Based on outstanding progresses in animal experiments, vaccines for some human tumors have been developed. However, clinical effects of these vaccines have been far below than expected. This discrepancy might come from differences between animal models and human patients with respect to immunocompetency. The immune status of mice after tumor inoculation has not been well studied, which make us cautious in interpreting and applying the results from mice to human. We evaluated cell-mediated immune responses in mice after tumor cell inoculation. Methods: Mice were inoculated with TA3Ha, CT26, or 4T1. Delayed-type hypersensitivity (DTH) responses were induced 2-4 weeks after inoculation using 2,4-dinitro-1-fluorobenzene as an antigen. The relationships between the severity of DTH responses and the duration of tumor inoculation or the size of tumor mass were analyzed. Results: In T A3Ha groups, DTH response was elevated 2 weeks after inoculation, but depressed after 4 weeks, compared to the control group. When analyzed based on the sizes of tumor masses elicited, DTH responses were inversely related to the mass size, especially in those greater than 10 mm in diameter. In CT26 groups, while the duration after inoculation did not affect the severity of DTH responses, those with large mass showed depressed responses regardless the duration of inoculation. 4T1 cells grew so slowly that the size of tumor mass was small even 4 weeks after inoculation, and this group showed much higher DTH responses compared to that of tumor-free group. Conclusion: At least in an experimental setting where tumor model was induced by inoculating tumor cell lines into immunologically competent mice, the host immune response was elevated in early stage, and then depressed in late stage when the mass grew over a critical size.

• IMMUNE NETWORK
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• 제6권2호
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• pp.59-66
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• 2006

Background: CM1 (Centrocyte/-blast Marker I) defined by a mAb developed against concanavalin-A activated PBMC, is expressed specifically on a subpopulation of centroblasts and centrocytes of human germinal center (GC) B cells. Burkitt lymphoma (BL) is a tumor consisting of tumor cells with the characteristics of GC B cell. Previously we reported that CM1 ligation with anti-CM1 mAb induced apoptosis in Ramos $(IgM^{high})$ and Raji $(IgM^{low})$ cells. Methods & Results: In the present study, we observed that CM1 ligation with anti-CM1 mAb induced Fas ligand and Fas expression in Ramos cells, but not in Raji cells. Furthermore, anti-Fas blocking antibody, ZB4, blocked CM1-mediated apoptosis effectively in Ramos cells, but not in Raji cells. Increased mitochondrial membrane permeabilization, which was measured by $DiOC_6$, was observed only in Raji cells. In contrast to no significant change of Bax known as pro-apoptotic protein, anti-apoptotic protein Bcl-2 was significantly decreased in Raji cells. In addition, we observed that CM1 ligation increased release of mitochondrial cytochrome c and upregulated caspase-9 activity in Raji cells. Conclusion: These results suggest that apoptosis induced by CM1-ligation is mediated by Fas-Fas ligand interaction in Ramos cells, whereas apoptosis is mediated by down-regulation of Bcl-2 and subsequent decrease of mitochondrial membrane potential in Raji cells.

• 한국전자파학회논문지
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• 제23권7호
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• pp.805-814
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• 2012

무선기기의 간헐적인 신호 또는 높고 낮은 진폭의 주기적인 신호 전송은 오디오 주파수에 간섭 신호를 발생시켜 근접 거리에 설치된 음향 기기에 잡음을 발생시킨다. 최근 이와 관련하여 Global system for Mobile Communication(GSM) 폰과 유사하게 간헐적인 신호 전송을 하는 Long Term Evolution(LTE) 폰에 의한 오디오 잡음현상이 보고되었다. 현재 국내 KN20 규격에서는 잡음에 대한 내성 능력을 확보하기 위하여 음향 및 TV 방송수신기의 내성 시험 기준 신호로 변조 주파수 1 kHz Amplitude Modulation(AM) 80 %와 Code Division Multiple Access(CDMA) 신호를 이용하고 있다. 본 논문에서는 LTE 폰의 출시에 따라 기존의 내성 기준 신호 AM과 CDMA를 GSM과 LTE 폰이 발생시키는 오디오 잡음의 수준과 비교한 후, 국내 음향 기기에 대한 내성 기준 신호의 개선을 제안한다. CDMA 신호는 음향 기기에 간섭을 거의 일으키지 않는 것으로 여겨지므로 시험 항목에서 삭제해도 무방할 것으로 판단된다. 반면, LTE는 많은 사용자가 이용하고 있으며, 음향 기기 등에 무시할 수 없는 간섭을 발생시킬 것으로 분석되므로 CDMA 신호를 대신하여 음향 기기 및 TV 방송 수신기의 내성 시험을 위한 시험 기준 신호로 규정하는 것이 바람직할 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제31권11호
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• pp.1583-1590
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• 2021

Studies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of Mycobacterium tuberculosis. However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this M. tuberculosis gene is not easily expressed in E. coli may be mainly due to the high content of G+C and the use of unique codons. Fusion tags are indispensable tools used to improve the soluble expression of recombinant proteins and accelerate the characterization of protein structure and function. In the present study, His6, Trx, and His6-MBP were used as fusion tags, but only MBP-PE_PGRS45 was expressed solubly. The purification using His6-MBP tag-specific binding to the Ni column was easy to separate after the tag cleavage. We used the purified PE_PGRS45 to immunize New Zealand rabbits and obtained anti-PE_PGRS45 serum. We found that the titer of polyclonal antibodies against PE_PGR45 was higher than 1:256000. The result shows that purified PE_PGRS45 can induce New Zealand rabbits to produce high-titer antibodies. In conclusion, the recombinant protein PE_PGRS45 was successfully expressed in E. coli and specific antiserum was prepared, which will be followed by further evaluation of these specific antigens to develop highly sensitive and specific diagnostic tests for tuberculosis.

• 한국가금학회지
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• 제38권4호
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• pp.275-284
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• 2011

As the world population grows and developing countries become more affluent, the global consumption of meat will increase by more than 50% within the next 10 years. Confronting the increased demand for poultry food products are emerging field diseases, increasing regulatory bans of antimicrobial growth promoters, high-density growth conditions, and waste management. Although biotechnology offers solutions to some of these challenges, basic studies are needed to better understand the complex interaction between the intestinal microbiome, host immunity and the environment. This presentation will focus on emerging strategies to enhance gut immunity and to decrease economic losses due to poultry diseases. This presentation will highlight recent developments in coccidiosis research and provide information on host immunity, immunomodulation, and the latest advances in dietary and nutritional approaches against coccidiosis. Such information will magnify our understanding of host-parasite biology, mucosal immunology, and design of future nutritional interventions and vaccination strategies for coccidiosis.

• 전자공학회논문지D
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• 제35D권9호
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• pp.54-61
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• 1998

Deep submicron급 CMOS디바이스에서 래치업 면역특성을 향상시키기 위한 새로운 Triple well구조를 제안하였다. Triple well에서 이온주입 에너지와 도즈량 변화에 따른 최적인 래치업 면역을 위한 공정조건을 확립하고 이것을 기존의 Twin well구조와 비교분석하였다. 공정은 공정시뮬레이터인 ATHENA로 소자를 제작하여 도핑프로파일과 구조를 해석하고 래치업 특성은 소자시뮬레이터인 ATLAS를 사용하였다. Triple well과 Twin well의 구조에서 공정상의 차이가 도핑프로파일에 미치는 영향과 프로파일 형태가 래치업 특성에 미치는 영향을 규명하였다. Triple well구조에서 p-well이온주입에너지 2.5MeV, 도즈량 1×10/sup 14/[cm/sup -2/]일 때 트리거 전류가 2.5[mA/${\mu}{m}$]로 매우 큰 래치업 면역특성을 얻었다.

• IMMUNE NETWORK
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• 제4권4호
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• pp.205-215
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• 2004

In the early host defense system, effector function of natural killer (NK) cells results in natural killing against target cells such as microbe-infected, malignant, and certain allogenic cells without prior stimulation. NK cell cytotoxicity is selectively regulated by homeostatic prevalence between a repertoire of both activating and inhibitory receptors, and the discrimination of untransformed cells is achieved by recognition of major histocompatibility complex (MHC) class I alleles through inhibitory signals. Although it is well known that the bipotential T/NK progenitors are derived from the common precusor, functional mechanisms in terms of the development of NK cells remain to be further investigated. NK cells are mainly involved in innate immunity, but recent studies have been reported that they also play a critical role in adaptive immune responses through interaction with dendritic cells (DC). This interaction will provide effector functions and development of NK cells, and elucidation of its precise mechanism may lead to therapeutic strategies for effective treatment of several immune diseases.