• Title/Summary/Keyword: image analyzer

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Gametogenic Cycle and the Number of Spawning Seasons by Quantitative Statistical Analysis, and the Size at 50% of Group Sexual Maturity in Atrina (Servatrina) pectinata (Bivalvia: Pinnidae) in Western Korea

  • Chung, Jae Seung;Chung, Ee-Yung;Lee, Chang-Hoon
    • The Korean Journal of Malacology
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    • v.28 no.4
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    • pp.363-375
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    • 2012
  • The gametogenic cycle, the number of spawning seasons per year and first sexual maturiity of the pen shell, Atrina (Servatrina) pectinata, were investigated by quantitative statistical analysis using an Image Analyzer System. Compared two previous results (the spawning periods in the reproductive cycles in 1998 and 2006) by qualitative histological analysis with the present results by quantitative statistical analysis, there are some differences in the spawning periods: the spawning period (June to September) by quantitative statistical analysis was one month longer than those of two previous reports (June to July or June to August) by qualitative histological analysis. However, the number of spawning seasons studied by the qualitative and quatitative analyses occurred once per year. In quantitative statistical analysis using an image analyzer system, the patterns of monthly changes in the percent (%) of the areas occupied by follicles to the ovary area in females (or that of the areas occupied by spermatogenic stages to the testis area in males) showed a maximum in May, and then sharply droped from June to September, 2006. From these data, it is apparent that the spawning season of A. (S.) pectinata occurred once a year from June to September, indicating a unimodal gametogenic cycle during the year. Shell heights of sexually mature pen shells (size at 50% of group sexual maturity, $GM_{50}$) that were fitted to an exponential equation were 15.81 cm in females and 15.72 cm in males (considered to be one year old).

Stationary and Moving Computed Radiography Grids : Comparative Observer's Perception (Computed Radiography에서 고정형 그리드와 이동형 그리드 영상의 인식률 비교)

  • Lee, Kiho;Lee, Changhoon;Jin, Gyehwan
    • Journal of the Korean Society of Radiology
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    • v.9 no.7
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    • pp.515-521
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    • 2015
  • This study assessed the degradation of image quality caused by grid artifacts and $moir{\acute{e}}$ pattern artifacts in a stationary grid, and the degradation of image quality caused by cut off artifacts in a moving grid. X-ray images were acquired in a stationary grid and a moving grid with X-ray exposure conditions of 100 cm, 80 kVp, and 30 mA using a CDRAD phantom and a 24 cm thickness acrylic phantom. Observer's perception of X-ray imaging using CDRAD Analyzer was mean 49.36, standard deviation 3.76, maximum 55.56, and minimum 38.67 in the stationary grid, and 47.04, 12.69, 55.56, and 20.89, respectively, in the moving grid. The stationary grid was superior to the moving grid in terms of the mean and standard deviation of observer's perception.

Composite Pressure Vessel for Natural Gas Vehicle by Filament Winding (필라멘트 와인딩 공정에 의한 천연가스 차량용 복합재료 압력용기)

  • 김병선;김병하;김진봉
    • Composites Research
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    • v.17 no.5
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    • pp.1-6
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    • 2004
  • Composite pressure vessels with HDPE (high density polyethlyne) liner with metal boss at each end were developed by Filament Winding Process. The vessel is composed of a dome-shaped part at each end and a cylinder-shaped part at the middle of the vessel. The environmental tests carried out for possible vessel materials such as High Density Polyethlyn (HDPE), resins and reinforcing fibers up to a year showed no significant damages. The boss was designed to minimize the gas leak which was verified by FEM analysis. Most ideal fiber tension was obtained by experimental method and the fiber volume fraction, $\textrm{V}_{f}$, obtained by image analyzer were 55.4 % in cylinder and 55.6 % in dome parts, respectively. Winding pattern is programmed to control the composite thickness in the dome areas such that the failure of the vessel may occur in the cylinder. During the cure, the vessel was rotated and a constant internal pressure of 0.62 bar was applied. From this, the vessel's burst pressure is improved by 28 %. The burst and fatigue tests for under-wound and fully wound vessel showed satisfactory results.

Genotoxicity of Taxol and 10-Deacetyl Baccatin III Using Single Cell Gel Electrophoresis (Comet Assay) in Chinese Hamster Lung Fibroblast

  • Kim, Hyun-Joo;Kim, Kyung-Ran;Youn, Ji-Youn;Kim, Min-Hee;Ryu, Jae-Chun
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 1996.12a
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    • pp.61-61
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    • 1996
  • Taxol is used as cancer therapeutic agent. It has been known as weak posotive of chromosome aberration assay in vitro in our previous results (Ryu et al., 1996) and potent clastogens in the mouse bone marrow micronucleus (Tinwell and Ashby, 1994). We performed microgel electrophoresis to determine the effect of taxol and it's precursor 10-deacetyl baccatin III(DAB) on DNA. Microgel electrophoresis is useful, rapid, simple, visual, and sensitive technique for measuring DNA breakage and repair mechanisms in mammalian 근ells. The range of concentration used for taxol were 854, 427, 213.5, 106.8, 53.4 Ug/ml, for DAB 910 ,455, 227.5 U9/ml, Cell viability always exceed 85%. We analyzed the results by using the special software of image analyzer for this comet assay (Komet 3.0). By using this image analyzer software , we can get the result as the tail moment ((mean of tail length - mean of head lengh) x tail%DNA/100). A slight increase in DNA migration was observed for taxol at the concentration of 854 Ug/m4 in the absence of S9 mixture. No increased DNA migration was observed after treatment with DAB.

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Formation of the multiamellar vesicles of AHAsomes and effect of removal on the horny layer (AHAsomes의 multilamellar vesicles형성과 각질제거 효과)

  • 김인영;서봉석
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.21 no.2
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    • pp.1-21
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    • 1995
  • In this context, it should be mentioned that multilamellar vesicles can be prepared with the main compounds of the intercellular lipids, ceramides, cholesterol, cholesteryl ester, squalane, lecithin, wax ester by effect of the wetting. We investigated properties formation of MLV with use of the AHAsomes and Microfluidizer. The multilamellar vesicles are formed merely adding polyol and water phase, followed with the microfluidizer. Formation of a practically pure AHAsomes system, containing the active ingredients directly incorporated without need for preservatives. There were very good encapsulated properties of the active ingredients whether hydrophilic(malic acid, tartaric acid, lactic acid, allantoin, urea) and hydrophobic(vitamin-I acetate, vitamin-A palmitate). Optimum condition (ormatiom of MLV was passed three times in the microfluidizer, particle size of the vesicles should be within range 50-523nm (mean=163.5nm). As application, It was compared that horny layer of the sole of foots removal with the general OM emulsion and the AHAsomes cream. There was used for three months, those got recovery wrinkles about 151.8% and elasticity three times more the AHAsomes than O/W emulsions, It was confirmed with the Image Analyzer and the Cutometer.

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A Study on the Morphological Analysis of Sperm (정자의 형태학적 특성 분석에 관한 연구)

  • Paick, Jae-Seung;Jeon, Seong-Soo;Kim, Soo-Woong;Yi, Won-Jin;Park, Kwang-Suk
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.2
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    • pp.153-165
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    • 1997
  • In male reproducible health, fertility and IVF (in-vitro fertilization), semen analysis has been most important. Semen analysis can be divided into concentration, motional and morphological analysis of sperm. The existing method which was developed earlier to analyze semen concentrated on the sperm motility analysis. To provide more useful and precise solutions for clinical problems such as infertility, semen analysis must include sperm morphological analysis. But the traditional tools for semen analysis are subjective, imprecise, inaccurate, difficult to standardize, and difficult to reproduce. Therefore, with the help of development of microcomputers and image processing techniques, we developed a new sperm morphology analyzer to overcome these problems. In this study the agreement on percent normal morphology was studied between different observers and a computerized sperm morphology analyzer on a slide-by-slide basis using strict criteria. Slides from 30 different patients from the SNUH andrology laboratory were selected randomly. Microscopic fields and sperm cells were chosen randomly and percent normal morphology was recorded. The ability of sperm morphology analyzer to repeat the same reading for normal and abnormal cells was studied. The results showed that there was no significant bias between two experienced observers. The limits of agreement were 4.1%${\sim}$-3.8%. The Pearson correlation coefficient between readers was 0.79. Between the manual and sperm morphology analyzer, the same findings were reported. In this experiments the slides were stained by two different methods, PAP and Diff-Quik staining methods. The limits of agreement were 7.2%${\sim}$-5.7% and 6.0%${\sim}$-6.3%, respectively. The Pearson correlation coefficients ware 0.76 and 0.91, respectively. The limits of agreement was tighter below 20% normal forms. In the experiments of repeatability, 52 cells stained by PAP and Diff-Quik staining methods were analyzed three times in succession. Estimating pairwise agreement, the kappa statistic for the pairs were 0.76, 0.81, 0.86, and 0.75, 0.88, 0.88 respectively. In this study it was shown that there was good agreement between manual and computerized assessment of normal and abnormal cells. The repeatability and agreement per slide of computerized sperm morphology analyzer was excellent. The computer's ability to classify normal morphology per slide is promising. Based on results obtained, this system can be of clinical value both in andrology laboratories and IVF units.

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Study for Organic(Bio)-Inorganic Nano-Hybrid OMC

  • Lee, Jung-Eun;Ji, Hong-Geun;Park, Yoon-Chang;Lee, Kyoung-Chul;Yoo, Eun-Ah
    • Proceedings of the SCSK Conference
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    • 2003.09a
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    • pp.178-191
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    • 2003
  • OMC is essentialiy necessary compound in sun goods as organic UV protecting products. But the skin-trouble problem is raising because of skin penetration of OMC. In this study, non-capsulated pure OMC was compared with Organic-Inorganic-Nano-hybrid OMC for skin penetration force and SPF degree. Organic- Inorganic Nano-Hybrid OMC is OMC trapped in the pore of the mesoporous silica synthesized by the sol-gel method after OMC is nanoemulsified in the system of the hydrogenated Lecithin/ Ethanol/caprylic/capric triglyceride/OMC/water. OMC- nano- emulsion was obtained by a microfluidizing process at 1000bar and then micelle size in the nanoemulsion solution is 100-200nm range. Mesoporous silica nano-hybrid OMC was prepared by the process; surfactant was added in dissolved OMC-Nanoemulsion, then the rod Micelle was formed. OMC-nanoemulsion was capsulated in this rod Micelle and then silica precursor was added in the OMC-nanoemulsion solution. Through the hydrolysis reaction of the silica precursor, mesoporous silica concluding OMC-Nanocapsulation was obtained. The nano-hybrid surface of this OMC-Nanoemulsion-Inorganic system was treated with polyalkyl-silane compound. OMC-Mesoporous silica Nano-hybrids coated with polyalkyl-silane compound show the higher sun protecting factor (SPF Analyzer: INDEX 10-15) than pure OMC and could reduce a skin penetration of OMC. The physico-chemical properties of these nano-hybrids measured on the SPF index, partical size, strcture, specific surface area, pore size, morphology, UV absorption, rate of the OMC dissolution using SPF Analyzer, Laser light scattering system, XRD, BET, SEM, chroma Meter, HPLC, Image analyzer, microfluidizer, UV/VIS. spectrometer.

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Development of Conformance Test Tools for Terrestrial DMB Broadcast Web Site Services (지상파 DMB 방송웹사이트 서비스 송수신 정합 시험 도구 개발)

  • Kim, Yong-Han;Moon, Su-Han;Chae, Young-Seok
    • Journal of Broadcast Engineering
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    • v.12 no.3
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    • pp.214-221
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    • 2007
  • In this paper, we presents conformance test tools for the broadcast web site (BWS) service which is one of the data services for terrestrial digital multimedia broadcasting (T-DMB). First, the methodology for the conformance test is established for both transmitters and receivers of T-DMB BWS services. The test tools consist of three parts: BWS bitstream analyzer, BWS reference decoder (software) module, and BWS test bitstreams. For testing transmitted BWS bitstreams, we developed the bitstream analyzer. For testing BWS receivers, we developed the reference Ifecoder module and created the test bitstreams. Actually the bitstream analyzer developed in this paper was used for the verification of the test bitstreams. The same test bitstreams are fed to a BWS decoder module under test and the reference decoder module and the results from the two are compared for verification. The test tools can be used for the test of all the protocol and textual/image specifications related to BWS including MOT protocol, HTML, PNG, MNG, JPEG, and Ecma Script.

Performance Analyzer for Embedded AI Processor (내장형 인공지능 프로세서를 위한 성능 분석기)

  • Hwang, Dong Hyun;Yoon, Young Hyun;Han, Chang Yeop;Lee, Seung Eun
    • Journal of Internet Computing and Services
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    • v.21 no.5
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    • pp.149-157
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    • 2020
  • Recently, as interest in artificial intelligence has increased, many studies have been conducted to implement AI processors. However, the AI processor requires functional verification as well as performance verification on whether the AI processor is suitable for the application. In this paper, We propose an AI processor performance analyzer that can verify the application performance and explore the limitations of the processor. By Using the performance analyzer, we explore the limitations of the AI processor and optimize the AI model to fit an AI processor in image recognition and speech recognition applications.

SPRAY STRUCTURE OF HIGH PRESSURE GASOLINE INJECTOR IN A GASOLINE DIRECT INJECTION ENGINE

  • Lee, Chang Sik;Chon, Mun Soo;Park, Young Cheol
    • International Journal of Automotive Technology
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    • v.2 no.4
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    • pp.165-170
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    • 2001
  • This study is focussed on the investigation of spray characteristics from the high pressure gasoline injector for the application of gasoline direct injection engine. For the analysis of spray structure of high pressure gasoline injector; the laser scattering method with a Nd-Yag laser and the Phase Doppler particle analyzer system were applied to observe the spray development and the measurement of the droplet size and velocity of the spray, respectively. Also spatial velocity distribution of the spray droplet was measured by use of the particle image velocity system. Experimental results show that high pressure gasoline injector shapes the hollow-cone spray, and produce the upward ring shaped vortex on the spray surface region. This upward ring shaped vortex promotes the secondary atomization of fuel droplets and contributes to a uniform distribution of fuel droplets. Most of fuel droplets are distributed under 31$\mu m$ of the mean droplet size (SMD) and the frequency distribution of the droplet size under 25$\mu m$ is over 95% at 7 MPa of injection pressure. According to the experimental results of PIV system, the flow patterns of the droplets velocity distribution in spray region are in good agreement with the spray macroscopic behaviors obtained from the visualization investigation.

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